Abstract：
:A technique is presented for isolating a subclass of lysosomes, designated crinosomes, by one-step floating-up centrifugation in a cesium-containing sucrose gradient. Rats were treated with vinblastine in order to induce the formation of crinosomes. Vinblastine blocked exocytosis of secretory granules at the cell border. Later on, lipoprotein-containing granules were seen throughout the cytoplasm. Immunolabeling with polyclonal antibodies against albumin demonstrated a severalfold greater presence of gold particles over crinosomes and Golgi cisternae than over the surrounding organelles. The induction of crinosomes by vinblastine made it possible to isolate thenm on a sucrose gradient. These organelles contained marker enzymes for the Golgi complex (galactosyl transferase) and lysosomes (cathepsins). The purity of the fraction was high. The crinosomes were proteolytically and lipolytically very active. The crinosomes were positive to cytochemical acid phosphatase staining. It is concluded that crinosomes develop from fusion between lysosomes and secretory granules and that they are active in degrading retained secretory material.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Glaumann H,Ahlberg J,Hultenby K,Jansson H,Mengarelli-Widholm S,Motakefi AMdoi
10.1016/0014-4800(89)90028-2subject
Has Abstractpub_date
1989-04-01 00:00:00pages
167-82issue
2eissn
0014-4800issn
1096-0945pii
0014-4800(89)90028-2journal_volume
50pub_type
杂志文章abstract：:Histoquantitative methods were applied to study the changes of splenic structure and proportions of splenic tissue compartments in cisplatin-treated (6 mg/kg body wt) male Wistar rats. Six days after treatment the significant reduction of volume density of white pulp was observed, which was due to the very prominent d...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1994.1027
更新日期：1994-10-01 00:00:00
abstract：:The maintenance of homeostasis of the immune system depends on the balance between excitatory and inhibitory signals. Programmed death ligand (PD-L1) is a molecule which downregulates the immune system targeting the programmed death receptor 1 (PD-1). Under physiological conditions, the receptor is constitutively expr...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章,评审
doi：10.1016/j.yexmp.2020.104494
更新日期：2020-10-01 00:00:00
abstract：:In this comparative study, rat hearts were perfused at 37 degrees C with three clearly defined protocols: the Ca2+ paradox, the O(2) paradox, and with 20 mM caffeine. Each protocol involved an initial priming (Ca(2+)(0) depletion or anoxia; stage 1) and subsequent full activation (Ca(2+)(0) repletion, caffeine or reox...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1999.2275
更新日期：1999-10-01 00:00:00
abstract：:Platelet homeostasis reflects a balance between the production of platelets via cytoplasmic fragmentation of megakaryocytes in the pulmonary microvasculature and their catabolism. Increased numbers of megakaryocytes are entrapped in the injured lung, potentially affecting circulating platelet counts. We enumerated pul...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2007.08.005
更新日期：2007-12-01 00:00:00
abstract：:There is a possibility that the aggresomes that form in the brain in neurodegenerative diseases like Alzheimer's disease (AD) and in the liver where aggresomes like Mallory-Denk Bodies (MDB) form, share mechanisms. MDBs can be prevented by feeding mice sadenosylmethionine (SAMe) or betaine. Possibly these proteins cou...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章,评审
doi：10.1016/j.yexmp.2016.03.010
更新日期：2016-06-01 00:00:00
abstract：:Heritable differences in tumor susceptibility are observed in mice after repetitive exposures to the organotropic colon carcinogen azoxymethane (AOM). The following study was undertaken to determine whether early morphological alterations within the colonic epithelium correlate with subsequent cancer risk. A/J and SWR...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/s0014-4800(99)80003-3
更新日期：1999-02-01 00:00:00
abstract：:Obliterative bronchiolitis is the major complication affecting long-term lung transplant survivors. Tumor necrosis factor-alpha (TNF-alpha) promotes inflammation and fibrosis in chronic lung injury models. These experiments defined the role of TNF-alpha in an established model of obliterative airway disease (OAD). Rat...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2004.10.008
更新日期：2005-06-01 00:00:00
abstract：:The influence of Kupffer cells (KC) from normal, D-galactosamine- and thioacetamide-injured liver on the growth of rat liver fat-storing cells (FSC) in culture was studied. The supplementation of FSC incubation medium with normal and galactosamine-KC-conditioned media for 4 days caused 1.5- and 1.9-fold increase (P le...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(88)90023-8
更新日期：1988-08-01 00:00:00
abstract：:Lumican is a member of a small leucine-rich proteoglycan (SLRP) family and it regulates the assembly and diameter of collagen fibers in the extracellular matrix of various tissues. Lumican expression was reported in various kinds of tumor cells. Lumican inhibits the growth of melanoma cells, but the lumican in pancrea...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2010.01.010
更新日期：2010-06-01 00:00:00
abstract：:The purpose of the study was to determine the temporal relationship between lymphocyte activating factor (LAF) activity and the acute-phase response, as measured by plasma fibronectin (Fn), C-reactive protein (CRP), and albumin levels in adjuvant arthritic rats. LAF activity as measured in the thymocyte costimulator a...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(88)90007-x
更新日期：1988-12-01 00:00:00
abstract：:In the present study, chemopreventive efficacy of hesperidin was evaluated against ferric nitrilotriacetate (Fe-NTA) induced renal oxidative stress and carcinogenesis in wistar rats. Nephrotoxicity was induced by single intraperitoneal injection of Fe-NTA (9 mg Fe/kg b.wt). Renal cancer was initiated by the administra...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2015.11.012
更新日期：2015-12-01 00:00:00
abstract：:Primary hepatic peripheral T-cell lymphoma (PTCL) is exceedingly rare. We encountered such a case in a 58-year-old Hispanic female with a history of chronic sinusitis and hypothyroidism who presented with 4weeks of fever and weight loss. Laboratory studies showed altered liver function and mild pancytopenia. Hepatitis...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2016.01.002
更新日期：2016-02-01 00:00:00
abstract：:Studies of spontaneous mutations in mice have provided valuable disease models and important insights into the mechanisms of human disease. Ruffled (rul) is a new autosomal recessive mutation causing abnormal hair coat in mice. The rul allele arose spontaneously in the RB156Bnr/EiJ inbred mouse strain. In addition to ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2015.01.015
更新日期：2015-04-01 00:00:00
abstract：:We tested the hypothesis that structural changes in the genome parallel age- and organ-specific phenotypes in conjunction with the differential transposition activities of retroelements. The genomes of the liver from C57BL/6J mice were larger than other organs, coinciding with an increase in genomic copies of certain ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2012.04.013
更新日期：2012-08-01 00:00:00
abstract：:Unilateral torsion of the spermatic cord was surgically induced in 24 Hartley strain guinea pigs. Groups of six animals were sacrificed 4, 8, 12, and 16 months after the surgery and testes were excised. Nine animals has severely damaged testes. Extensive study on the Sertoli cells of each of these nine animals was car...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(86)90072-9
更新日期：1986-04-01 00:00:00
abstract：:CD44 is a polymorphic family of immunologically related integral membrane glycoproteins associated with cell matrix adhesion, lymphocyte activation and targeting, and tumor growth and metastasis. We studied CD44 expression in 114 formalin-fixed paraffin-embedded thyroid tumors using the A3D8 anti-human CD44 monoclonal...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1994.1037
更新日期：1994-12-01 00:00:00
abstract：:The hamster cheek pouch is an excellent target tissue for the experimental study of oral carcinogenesis. In the course of searching for molecular alterations during the malignant transformation process, the necessity for a molecular marker for cellular proliferation became apparent. In this report, we show that the ce...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(88)90034-2
更新日期：1988-10-01 00:00:00
abstract：:FineFix, RCL-2 and HOPE, three formalin-free fixatives, were compared to the common used formalin fixed tissue samples of lung cancer and were evaluated for their effects on quality, quantity and integrity of RNA and microRNA. Two commercially available RNA extraction Kits (RNeasy FFPE by Qiagen and RecoverAll™ Nuclei...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2011.05.007
更新日期：2011-10-01 00:00:00
abstract：:Granulomas, the pathologic hallmarks of tuberculosis, are composed of tightly numerous immune cells that respond to a variety of persistent stimuli during pathogen-host interaction. The granuloma is essential for host containment of mycobacterial infection, however, the mechanism of host and pathogen determinants to r...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2012.05.004
更新日期：2012-10-01 00:00:00
abstract：:The morphology of Gleason 4 prostate cancer (PCa) can be subdivided into cribriform and non-cribriform patterns. A large body of evidence has shown that pattern 4 cribriform PCa (especially non-glomeruloid type) is associated with adverse pathologic features and clinical outcomes compared with non-cribriform pattern 4...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2020.104400
更新日期：2020-06-01 00:00:00
abstract：:An immunohistochemical technique was used to localize the major constitutive cytochrome P450 isozyme, P450 LM2, and the major beta-naphthoflavone-inducible isozyme, P450 LM4b, in the livers of untreated and aflatoxin B1 (AFB1)-initiated, tumor-bearing rainbow trout. In hepatic tissue sections from untreated trout, no ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(89)90044-0
更新日期：1989-06-01 00:00:00
abstract：:We have produced experimental diffuse interstitial pulmonary fibrosis in rats with a combination of low and repeated doses of paraquat plus continuous exposure to normobaric 74% O2 in the breathing air for several weeks. Pulmonary fibrosis was evaluated histologically and biochemically, through the determination of to...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(85)90074-7
更新日期：1985-12-01 00:00:00
abstract：:We have modified methods of growing human gallbladder epithelial cells in monolayer and organotypic culture. These cells were grown in the presence of fetal bovine serum and with coculture of feeder layers of human gallbladder fibroblasts. Human gallbladders were obtained from cholecystectomy specimens, and the cells ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1995.1026
更新日期：1995-08-01 00:00:00
abstract：:RNA binding motif protein 3 (RBM3) has been shown to be upregulated in several types of human tumors. Using tissue microarrays and immunohistochemistry, we showed here that both nuclear and cytoplasmic RBM3 expression levels were higher in hepatocellular carcinoma (HCC) tissues than in adjacent non-tumorous tissues. H...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2020.104546
更新日期：2020-12-01 00:00:00
abstract：:A primary culture of cells derived from uninvolved and atherosclerotic intima of human aorta was used to elucidate the role of cyclic nucleotides in atherogenesis. The cells cultured from fatty streaks and atherosclerotic plaques had a 2- to 8-fold lower cyclic AMP level and a 1.5- to 2-fold higher level of cyclic GMP...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(87)90020-7
更新日期：1987-12-01 00:00:00
abstract：:One of the models that best explains the cellular heterogeneity observed in central nervous system (CNS) tumors is the presence of cancer stem cells (CSCs). CSCs can originate from differentiated adult cells that return to an undifferentiated stage through the mechanism known as epithelial-mesenchymal transition (EMT)...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2020.104520
更新日期：2020-10-01 00:00:00
abstract：OBJECTIVES:Ethanol has been considered as a lifestyle risk factor for cancer in humans. While some studies have indicated that alcohol intake has a preventive effect for renal cell cancer, others have not. The metabolism of alcohol in cancer cells may be in many ways different than in healthy tissue and its disturbance...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2015.03.012
更新日期：2015-06-01 00:00:00
abstract：:The pathophysiological biology of human hypertrophic scar was examined in a long-term organ culture system. Fresh full-thickness, thin slices of scar were placed in petri dishes. Tissue was successfully maintained for 2 weeks in an environment made up of CMRL-1066 medium, fetal bovine serum, insulin, and hydrocortison...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(84)90079-0
更新日期：1984-04-01 00:00:00
abstract：:In a previous study the authors demonstrated monotypic foci, all of timidus type, in the atherosclerotic lesions and normal aortic tissue of glucose-6-phosphate dehydrogenase (G-6-PD) mosaic hares fed 25-hydroxycholesterol or triol in addition to a high-cholesterol diet. Other hares fed the high-cholesterol diet witho...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(85)90019-x
更新日期：1985-02-01 00:00:00
abstract：BACKGROUND:The increased expression of the fibroblast growth factor receptor 4 (FGFR4) has been identified in many human cancers. Recently, a single nucleotide polymorphism changing the sense codon 388 from glycine to arginine was identified in the FGFR4 gene. The FGFR4 Arg(388) allele was found to be associated with a...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2006.05.003
更新日期：2007-02-01 00:00:00
