当前位置： SCI文献检索 > EXPERIMENTAL AND MOLECULAR PATHOLOGY期刊下所有文献 > Evaluation of formalin-free tissue fixation for RNA and microRNA studies.

Evaluation of formalin-free tissue fixation for RNA and microRNA studies.

Abstract：

:FineFix, RCL-2 and HOPE, three formalin-free fixatives, were compared to the common used formalin fixed tissue samples of lung cancer and were evaluated for their effects on quality, quantity and integrity of RNA and microRNA. Two commercially available RNA extraction Kits (RNeasy FFPE by Qiagen and RecoverAll™ Nucleic Acid Isolation by Ambion) were tested and optimized in order to determine an extraction protocol for RNA as well as miRNA independent of the fixative. Two selected miRNAs were quantified via TaqMan MicroRNA assays. The optimized RNA extraction protocol for Qiagen's Kit leads to similar results for RNA quality and integrity for all fixatives. Highest RNA yield was obtained for formalin and the highest average miRNA ratio was found for FineFix. RNA fragments smaller than 500 bases were detected in FineFix, formalin and RCL2 fixed tissues; HOPE was the only fixative showing long fragments in one third of the samples. Our findings demonstrate that formalin-free fixatives are in general not superior for RNA studies. With our optimized RNA extraction protocol, there is no difficulty in extracting great amounts of RNA with high quality. According to the quality obtained, quantitative real-time PCR analysis can be performed without any negative impact. Similar results can be achieved for the tested fixatives and therefore no fixative seems to represent a new "gold-standard" for tissue fixation.

journal_name

Exp Mol Pathol

journal_title

Experimental and molecular pathology

authors

Arzt L,Kothmaier H,Quehenberger F,Halbwedl I,Wagner K,Maierhofer T,Popper HH

doi

10.1016/j.yexmp.2011.05.007

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

490-5

issue

2

eissn

0014-4800

issn

1096-0945

pii

S0014-4800(11)00065-7

journal_volume

91

pub_type

杂志文章

相关文献

EXPERIMENTAL AND MOLECULAR PATHOLOGY文献大全
  • Antiandrogen abiraterone and docetaxel treatments affect Notch1, Jagged1 and Hes1 expressions in metastatic prostate cancer cells.

    abstract：BACKGROUND:Prostate cancer is the most common cancer in men. A Notch signaling pathway is an important pathway in cell proliferation, differentiation, and fate. However, currently, the effects of abiraterone based-anti-androgene therapy and docetaxel, the most commonly used standard chemotherapy in prostate cancer trea...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2021.104607

    authors： Soylu H,Kırca M,Avcı S,Ozpolat B,Ustunel I

    更新日期：2021-01-19 00:00:00

  • Protective effect of ischemic preconditioning on the jejunal graft mucosa injury during cold preservation.

    abstract：:Protection of intestinal graft mucosa during cold preservation is still an unmet need in clinical practice, thus affecting the success of transplantation. The present study investigates the ability of two ischemic preconditioning (IPC) procedures to limit cold preservation injury. Three groups of Sprague-Dawley rats w...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2015.06.020

    authors： Jonecova Z,Toth S,Maretta M,Ciccocioppo R,Varga J,Rodrigo L,Kruzliak P

    更新日期：2015-10-01 00:00:00

  • Discovery of two novel EWSR1/ATF1 transcripts in four chimerical transcripts-expressing clear cell sarcoma and their quantitative evaluation.

    abstract：:The most common recurrent translocation in clear cell sarcoma t(12;22)(q13;q12) results in an EWSR1/ATF1 chimeric gene. We present a molecular analysis of tumor overgrowing right proximal tibia with bone destruction metastatic to two groin lymph nodes. Fluorescent in situ hybridization analysis performed on paraffin-e...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2010.12.005

    authors： Jakubauskas A,Valceckiene V,Andrekute K,Seinin D,Kanopka A,Griskevicius L

    更新日期：2011-04-01 00:00:00

  • Role of homologous recombination in carcinogenesis.

    abstract：:Cancer develops when cells no longer follow their normal pattern of controlled growth. In the absence or disregard of such regulation, resulting from changes in their genetic makeup, these errant cells acquire a growth advantage, expanding into precancerous clones. Over the past decade many studies have revealed the r...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章,评审

    doi：10.1016/s0014-4800(03)00010-8

    authors： Bishop AJ,Schiestl RH

    更新日期：2003-04-01 00:00:00

  • Analysis of point mutations and copy number variation in Grade II and III meningioma.

    abstract：:Meningiomas are among the most common tumors of the adult central nervous system (CNS). They are classified by the World Health Organization into three pathologic grades with increasing severity: grade I are benign with favorable treatment outcomes and low recurrence rates while grade III display malignant behavior an...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2018.10.007

    authors： McNulty SN,Schwetye K,Goldstein M,Carter J,Schmidt RE,Ansstas G,Tsien CI,Kim AH,Dahiya S

    更新日期：2018-12-01 00:00:00

  • Eotaxin/CCL11 expression by infiltrating macrophages in rat heart transplants during ongoing acute rejection.

    abstract：:Eotaxin/CCL11 chemokine is expressed in different organs, including the heart, but its precise cellular origin in the heart is unknown. Eotaxin is associated with Th2-like responses and exerts its chemotactic effect through the chemokine receptor-3 (CCR3), which is also expressed on mast cells (MC). The aim of our stu...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2009.07.006

    authors： Zweifel M,Mueller C,Schaffner T,Dahinden C,Matozan K,Driscoll R,Mohacsi P

    更新日期：2009-10-01 00:00:00

  • Immunohistochemical versus molecular detection of RAK antigens in breast cancer.

    abstract：:RAK antigens p120, p42, and p25 exhibit molecular and immunological similarity to the proteins encoded by HIV-1 and are expressed by 95% of breast and gynecological cancer cases in women and prostate cancer cases in men. Binding of the monoclonal antibody (MAb) RAK-BrI to cancer RAK antigens has been found to be inhib...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1006/exmp.2000.2310

    authors： Kyriacou KC,Iacovou F,Adamou A,Hadjisavvas A,Rakowicz-Szulczynska EM

    更新日期：2000-08-01 00:00:00

  • Concordance of DNA methylation pattern in plasma and tumor DNA of Egyptian hepatocellular carcinoma patients.

    abstract：:Hepatocellular carcinoma (HCC) is the fifth most common cancer and the fourth leading cause of cancer mortality globally. HCC incidence has doubled in Egypt in the past 10 years, which could be attributed to the high prevalence of hepatitis C virus (HCV) and hepatitis B virus (HBV), although HBV rates have declined af...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2009.09.012

    authors： Iyer P,Zekri AR,Hung CW,Schiefelbein E,Ismail K,Hablas A,Seifeldin IA,Soliman AS

    更新日期：2010-02-01 00:00:00

  • EGFRvIII epigenetically regulates ARHI to promote glioma cell proliferation and migration.

    abstract：:Epidermal growth factor receptor variant III (EGFRvIII) is a tumor-specific mutation widely expressed in glioma. However, its role and molecular mechanism in glioma have not been completely elucidated. Immunohistochemistry analyses of EGFRvIII, enhancer of zeste homolog 2 (EZH2) and aplysia ras homolog I (ARHI) were p...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2019.104344

    authors： Zheng Y,Ma Y,Yue H,Liu G,Han S

    更新日期：2020-02-01 00:00:00

  • Neurotoxicity of dichlorvos: effect on antioxidant defense system in the rat central nervous system.

    abstract：:The effect of dichlorvos exposure (5 mg kg-1 body wt, ip) on lipid peroxidation and antioxidant defense system in different regions of the rat central nervous system was studied. In the present paper an inhibition of acetylcholinesterase activity was used as an index of dichlorvos neurotoxicity. We observed significan...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(92)90031-6

    authors： Julka D,Pal R,Gill KD

    更新日期：1992-04-01 00:00:00

  • Poly (ADP) ribose synthetase inhibition in alveolar macrophages undergoing hypoxia and reoxygenation.

    abstract：BACKGROUND:Inhibition of the nuclear enzyme poly ribose synthetase (PARS) protects against in vivo lung ischemia reperfusion injury (LIRI). The effectiveness of intratracheal treatment suggests that PARS inhibition may primarily modulate alveolar macrophage (AM) activation. These studies attempted to characterize the e...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2007.11.005

    authors： McCourtie AS,Farivar AS,Woolley SM,Merry HE,Wolf PS,Szabo C,Mulligan MS

    更新日期：2008-04-01 00:00:00

  • Cyclic nucleotides and atherosclerosis: studies in primary culture of human aortic cells.

    abstract：:A primary culture of cells derived from uninvolved and atherosclerotic intima of human aorta was used to elucidate the role of cyclic nucleotides in atherogenesis. The cells cultured from fatty streaks and atherosclerotic plaques had a 2- to 8-fold lower cyclic AMP level and a 1.5- to 2-fold higher level of cyclic GMP...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(87)90020-7

    authors： Tertov VV,Orekhov AN,Kudryashov SA,Klibanov AL,Ivanov NN,Torchilin VP,Smirnov VN

    更新日期：1987-12-01 00:00:00

  • Chemopreventive efficacy of hesperidin against chemically induced nephrotoxicity and renal carcinogenesis via amelioration of oxidative stress and modulation of multiple molecular pathways.

    abstract：:In the present study, chemopreventive efficacy of hesperidin was evaluated against ferric nitrilotriacetate (Fe-NTA) induced renal oxidative stress and carcinogenesis in wistar rats. Nephrotoxicity was induced by single intraperitoneal injection of Fe-NTA (9 mg Fe/kg b.wt). Renal cancer was initiated by the administra...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2015.11.012

    authors： Siddiqi A,Hasan SK,Nafees S,Rashid S,Saidullah B,Sultana S

    更新日期：2015-12-01 00:00:00

  • Reduced FHIT expression is associated with tumor progression in sporadic colon adenocarcinoma.

    abstract：PURPOSE:Tumor supressor gene FHIT was identified at chromosome 3p14.2 spanning the FRA3B fragile site and is very often inactivated in different types of cancer. The aim of this study was to examine the frequency of FHIT gene LOH as well as FHIT mRNA and protein expression in sporadic colon adenocarcinoma. METHODS:The...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2013.12.005

    authors： Kapitanović S,Čačev T,Lončar B,Catela Ivković T,Križanac Š,Pavelić K

    更新日期：2014-02-01 00:00:00

  • Growth and differentiation factor-5 (GDF-5) in the human intervertebral annulus cells and its modulation by IL-1ß and TNF-α in vitro.

    abstract：:Growth and differentiation factor-5 (GDF-5) is a member of the TGF-ß superfamily which regulates cell division and differentiation. GDF-5 attracted high interest because of its role in skeletal development, especially in cartilaginous sites. Little is known, however, about the role of GFD-5 in disc cell biology. The p...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2014.02.005

    authors： Gruber HE,Hoelscher GL,Ingram JA,Bethea S,Hanley EN Jr

    更新日期：2014-04-01 00:00:00

  • In human epidermis, beta-defensin 2 is packaged in lamellar bodies.

    abstract：:The skin presents a mechanical, as well as an immunological barrier to infection, and displays considerable innate immune capacity. Recently, cultured human keratinocytes were described to produce and export a microbicidal peptide human beta-defensin 2 (HBD-2). Immunogold was used to label ultrathin cryosections of st...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/s0014-4800(02)00023-0

    authors： Oren A,Ganz T,Liu L,Meerloo T

    更新日期：2003-04-01 00:00:00

  • c-Yes enhances tumor migration and invasion via PI3K/AKT pathway in epithelial ovarian cancer.

    abstract：:Overexpression of c-Yes has been noted to correlation with several human cancers. However, the effects of c-Yes on epithelial ovarian cancer (EOC) development remain unclear. The aim of this study is going to prove the effects of c-Yes and related mechanisms in proliferation, metastasis and invasion of EOC. Immunohist...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2016.06.002

    authors： Jin Y,Huang M,Wang Y,Yi C,Deng Y,Chen Y,Jiang L,Wang J,Shen Q,Liu R,QinghuaXi

    更新日期：2016-08-01 00:00:00

  • Expression dynamics of drug resistance genes, multidrug resistance 1 (MDR1) and lung resistance protein (LRP) during the evolution of overt leukemia in myelodysplastic syndromes.

    abstract：:It is well-known that leukemic cells of overt leukemia (OL) that have transformed from myelodysplastic syndromes (MDS) are more resistant to chemotherapy as compared with de novo AML cells. Thus, to examine the expression levels of drug-resistant genes and their alterations with the development of OL in MDS, the expre...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2006.01.004

    authors： Kurata M,Hasegawa M,Nakagawa Y,Abe S,Yamamoto K,Suzuki K,Kitagawa M

    更新日期：2006-12-01 00:00:00

  • Dengue fever and bone marrow myelofibrosis.

    abstract：:Myelofibrosis is characterized by reticulin and/or collagen fibrosis in the bone marrow stroma resulting in secondary cytopenia. In addition to clonal hematologic neoplasms, myelofibrosis may also develop in association with other clinical conditions, including hematological disorders, solid malignancies, Down syndrom...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2014.07.004

    authors： Qing X,Sun N,Yeh J,Yue C,Cai J

    更新日期：2014-10-01 00:00:00

  • Clinical significance of 21-gene recurrence score assay for hormone receptor-positive, lymph node-negative breast cancer in early stage.

    abstract：OBJECTIVE:To identify the relationship between clinical pathological characteristics and the recurrence score (RS) on a 21-gene expression assay in patients with hormone receptor-positive, node-negative breast cancer, as well as the effect of RS on adjuvant decision-making. METHODS:The retrospective study was conducte...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2019.04.013

    authors： Yu-Qing Y,Lei W,Mei-Ling H,Jing-Jing X,Mei-Chen W,Jiang W,Jun-Sheng H,Rui L,Nan-Lin L

    更新日期：2019-06-01 00:00:00

  • Obliterative airway disease in rat tracheal allografts requires tumor necrosis factor alpha.

    abstract：:Obliterative bronchiolitis is the major complication affecting long-term lung transplant survivors. Tumor necrosis factor-alpha (TNF-alpha) promotes inflammation and fibrosis in chronic lung injury models. These experiments defined the role of TNF-alpha in an established model of obliterative airway disease (OAD). Rat...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2004.10.008

    authors： Farivar AS,Mackinnon-Patterson B,McCourtie AS,Namkung J,Ward PA,Mulligan MS

    更新日期：2005-06-01 00:00:00

  • Chemerin is highly expressed in hepatocytes and is induced in non-alcoholic steatohepatitis liver.

    abstract：:Chemerin is a recently described adipokine whose adipose tissue and serum levels are increased in obesity. Chemerin is expressed in the liver, and here, expression of chemerin has been studied in liver cells and in non-alcoholic fatty liver disease (NAFLD) which is more often found in obesity. Chemerin is shown to be ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2013.07.009

    authors： Krautbauer S,Wanninger J,Eisinger K,Hader Y,Beck M,Kopp A,Schmid A,Weiss TS,Dorn C,Buechler C

    更新日期：2013-10-01 00:00:00

  • Copper toxicosis and tolerance in the rat. III. Intracellular localization of copper in the liver and kidney.

    abstract：:Male rats fed a 3 g/kg copper diet were killed sequentially up to 14 weeks. Copper in liver and kidney cortex was identified histochemically and assayed in homogenates and gradient fractions following analytical subcellular fractionation on reorientating sucrose density gradients. Copper accumulated fastest in the liv...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(85)90041-3

    authors： Haywood S,Loughran M,Batt RM

    更新日期：1985-10-01 00:00:00

  • The morphological development of glycol ether-induced testicular atrophy in the rat.

    abstract：:The testicular effects of daily oral dosing with two glycol ethers--either ethylene glycol monomethyl ether (EGM) or monoethyl ether (EGE) were studied in the prepubertal rat by histological examination of the testes. Over the 11-day dosing period studied, EGM was found to produce testicular damage at dose levels of a...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(84)90074-1

    authors： Creasy DM,Foster PM

    更新日期：1984-04-01 00:00:00

  • Cytokine levels in HIV infected and uninfected Indian women: correlation with other STAs.

    abstract：:Host immune status is an important determinant of disease progression. Infections in the genital tract may alter the immunity in the particular site and hence affect the production of local cytokines. We performed this study to determine whether HIV in association with cervical HPV and CT/GC infections influences the ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2008.10.001

    authors： Guha D,Chatterjee R

    更新日期：2009-02-01 00:00:00

  • The pancreas in the degu.

    abstract：:Unique alpha-cell crystals, a herpes-like virus, islet amyloidosis, and immunohistochemical reactions of islets are compared in the rodent, Octodon degus, in animals with ordinary and high circulating glucose levels. Results suggest that crystals, virus, and amyloid are independent of blood sugar and bear no obvious r...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(84)90047-9

    authors： Spear GS,Caple MV,Sutherland LR

    更新日期：1984-06-01 00:00:00

  • Tubular cell senescence and expression of TGF-beta1 and p21(WAF1/CIP1) in tubulointerstitial fibrosis of aging rats.

    abstract：:Kidney aging has been recognized as a chronic process of compromised renal function and structural changes in the tubulointerstitium and glomerulus. Cell senescence is associated with alterations in cell structure and function, including expression of cytokines and structural and regulatory components of extracellular...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1006/exmp.2000.2346

    authors： Ding G,Franki N,Kapasi AA,Reddy K,Gibbons N,Singhal PC

    更新日期：2001-02-01 00:00:00

  • LncRNA KCNQ1OT1 contributes to cardiomyocyte apoptosis by targeting FUS in heart failure.

    abstract：:Long noncoding RNAs (lncRNAs) have recently been recognized as the important regulators in cardiac diseases. This study was aimed to investigate the role and molecular mechanism of lncRNA KCNQ1OT1 in regulating cardiomyocyte apoptosis in heart failure (HF). The mouse model of HF was induced by doxorubicin (ADR). Cell ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2020.104480

    authors： Lai L,Xu Y,Kang L,Yang J,Zhu G

    更新日期：2020-08-01 00:00:00

  • High flow drives vascular endothelial cell proliferation during flow-induced arterial remodeling associated with the expression of vascular endothelial growth factor.

    abstract：:Endothelial cell activation and proliferation are the essential steps in flow-induced arterial remodeling. We investigated endothelial cell turnover in the early stages of high-flow in the rabbit common carotid arteries using an arteriovenous fistula (AVF) model by kinetic investigation of cell proliferation and cell ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/s0014-4800(03)00032-7

    authors： Sho E,Komatsu M,Sho M,Nanjo H,Singh TM,Xu C,Masuda H,Zarins CK

    更新日期：2003-08-01 00:00:00

  • Evaluation of gene expression related to hepatic cell maturation and differentiation in a chemically induced mouse hepatoblastoma cell line.

    abstract：:The MHB-2 cell line, established from a mouse hepatoblastoma (HB), was subjected to the reverse transcriptase-polymerase chain reaction (RT-PCR) for evaluation of gene expression related to cell differentiation. RNAs for c-kit, CD34, thy-1, albumin, cytokeratin (CK) 8, 18 and 19 could be detected, but expression of al...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2007.05.004

    authors： Sakairi T,Okada M,Ikeda I,Utsumi H,Kohge S,Sugimoto J,Sano F,Takagi S

    更新日期：2007-12-01 00:00:00