Two-photon fluorescence imaging and bimodal phototherapy of epidermal cancer cells with biocompatible self-assembled polymer nanoparticles.

Abstract:

:We have developed herein an engineered polymer-based nanoplatform showing the convergence of two-photon fluorescence imaging and bimodal phototherapeutic activity in a single nanostructure. It was achieved through the appropriate choice of three different components: a β-cyclodextrin-based polymer acting as a suitable carrier, a zinc phthalocyanine emitting red fluorescence simultaneously as being a singlet oxygen ((1)O2) photosensitizer, and a tailored nitroaniline derivative, functioning as a nitric oxide (NO) photodonor. The self-assembly of these components results in photoactivable nanoparticles, approximately 35 nm in diameter, coencapsulating a multifunctional cargo, which can be delivered to carcinoma cells. The combination of steady-state and time-resolved spectroscopic and photochemical techniques shows that the two photoresponsive guests do not interfere with each other while being enclosed in their supramolecular container and can thus be operated in parallel under control of light stimuli. Specifically, two-photon fluorescence microscopy allows mapping of the nanoassembly, here applied to epidermal cancer cells. By detecting the red emission from the phthalocyanine fluorophore it was also possible to investigate the tissue distribution after topical delivery onto human skin ex vivo. Irradiation of the nanoassembly with visible light triggers the simultaneous delivery of cytotoxic (1)O2 and NO, resulting in an amplified cell photomortality due to a combinatory effect of the two cytotoxic agents. The potential of dual therapeutic photodynamic action and two-photon fluorescence imaging capability in a single nanostructure make this system an appealing candidate for further studies in biomedical research.

journal_name

Biomacromolecules

journal_title

Biomacromolecules

authors

Kandoth N,Kirejev V,Monti S,Gref R,Ericson MB,Sortino S

doi

10.1021/bm500156z

subject

Has Abstract

pub_date

2014-05-12 00:00:00

pages

1768-76

issue

5

eissn

1525-7797

issn

1526-4602

journal_volume

15

pub_type

杂志文章
  • Horseradish peroxidase-catalyzed polymerization of cardanol in the presence of redox mediators.

    abstract::Horseradish peroxidase-catalyzed polymerization of cardanol in aqueous organic solvent was investigated in the presence of a redox mediator. Cardanol is a phenol derivative from a renewable resource mainly having a C15 unsaturated hydrocarbon chain with mostly 1-3 double bonds at a meta position. Unlike soybean peroxi...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm034325u

    authors: Won K,Kim YH,An ES,Lee YS,Song BK

    更新日期:2004-01-01 00:00:00

  • Synthesis and characterization of biodegradable cationic poly(propylene fumarate-co-ethylene glycol) copolymer hydrogels modified with agmatine for enhanced cell adhesion.

    abstract::We synthesized positively charged biodegradable hydrogels by cross-linking of agmatine-modified poly(ethylene glycol)-tethered fumarate (Agm-PEGF) and poly(propylene fumarate-co-ethylene glycol) (P(PF-co-EG)) to investigate the effect of the guanidino groups of the agmatine on hydrogel swelling behavior and smooth mus...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm0200433

    authors: Tanahashi K,Jo S,Mikos AG

    更新日期:2002-09-01 00:00:00

  • Self-association of mucin.

    abstract::Aggregation phenomena in aqueous solutions of purified human tracheobronchial mucin have been studied by rheological methods, steady-state fluorescence, quasielastic light scattering, and spin probe techniques. At temperatures below 30 degrees C and concentrations above 15 mg/mL and in the absence of chaotropic agents...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm005532m

    authors: Bromberg LE,Barr DP

    更新日期:2000-10-01 00:00:00

  • Biomimetically mineralized salmon collagen scaffolds for application in bone tissue engineering.

    abstract::Biomimetic mineralization of collagen is an advantageous method to obtain resorbable collagen/hydroxy-apatite composites for application in bone regeneration. In this report, established procedures for mineralization of bovine collagen were adapted to a new promising source of collagen from salmon skin challenged by t...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm201776r

    authors: Hoyer B,Bernhardt A,Heinemann S,Stachel I,Meyer M,Gelinsky M

    更新日期:2012-04-09 00:00:00

  • SEC-MALS-QELS study on the molecular conformation of cellulose in LiCl/amide solutions.

    abstract::The SEC-MALS-QELS (size-exclusion chromatography equipped with multiangle light scattering and quasi-elastic light scattering detectors) method using lithium chloride/N,N-dimethylacetamide (LiCl/DMAc) and LiCl/1,3-dimethyl-2-imidazolidinone (LiCl/DMI) as mobile phases was applied to cellulose and cellulose tricarbanil...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm049386m

    authors: Yanagisawa M,Isogai A

    更新日期:2005-05-01 00:00:00

  • Triblock Copolymer Nanovesicles for pH-Responsive Targeted Delivery and Controlled Release of siRNA to Cancer Cells.

    abstract::New pH-responsive polymersomes for active anticancer oligonucleotide delivery were prepared from triblock copolymers. The delivery systems were formed by two terminal hydrophilic blocks, PEG and polyglycerolmethacrylate (poly-GMA), and a central weakly basic block, polyimidazole-hexyl methacrylate (poly-ImHeMA), which...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/acs.biomac.5b00286

    authors: Gallon E,Matini T,Sasso L,Mantovani G,Armiñan de Benito A,Sanchis J,Caliceti P,Alexander C,Vicent MJ,Salmaso S

    更新日期:2015-07-13 00:00:00

  • Conformational changes and molecular mobility in plasticized proteins.

    abstract::Most biopolymers exist in a plasticized state, whether it is naturally with water or unnaturally with glycerol or other suitable polyol, to make a flexible material. We have found that the extent to which a biopolymer can be plasticized is dependent on its molecular and higher order structures outside of simply molecu...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm800759g

    authors: Athamneh AI,Griffin M,Whaley M,Barone JR

    更新日期:2008-11-01 00:00:00

  • Thermodynamic Analysis of the Interaction of Heparin with Lysozyme.

    abstract::Glycosaminoglycan (GAG)-protein binding governs critically important signaling events in living matter. Aiming at a quantitative analysis of the involved processes, we herein present a thermodynamic study of the interaction of the model GAG heparin and lysozyme in aqueous solution. Heparin is a highly charged linear p...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/acs.biomac.0c00780

    authors: Walkowiak JJ,Ballauff M,Zimmermann R,Freudenberg U,Werner C

    更新日期:2020-11-09 00:00:00

  • Characteristics of novel dental composites containing 2,2-bis[4-(2-methoxy-3-methacryloyloxy propoxy) phenyl] propane as a base resin.

    abstract::Many dental restorative dental composites still utilize 2,2-bis[4-(2-hydroxy-3-methacryloyloxy propoxy) phenyl] propane (Bis-GMA) as base resin. The high viscosity of Bis-GMA necessitates dilution with dimethacrylate ethers of low viscosity such as triethylene glycol dimethacrylate (TEGDMA). However, increased amounts...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm050491l

    authors: Kim JW,Kim LU,Kim CK,Cho BH,Kim OY

    更新日期:2006-01-01 00:00:00

  • AC conductivity of selectively located carbon nanotubes in poly(epsilon-caprolactone)/polylactide blend nanocomposites.

    abstract::DC and AC electrical conductivity of bionanocomposites based on the immiscible polymer blend poly(epsilon-caprolactone)/polylactide (PCL/PLA, w/w 70/30), loaded with multiwall carbon nanotubes (CNT), were studied in a wide frequency range, 10(-3) < or = f < or = 10(7) Hz from 143 to 313 K. The nanofiller concentration...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm100135n

    authors: Laredo E,Grimau M,Bello A,Wu DF,Zhang YS,Lin DP

    更新日期:2010-05-10 00:00:00

  • Hepatocyte cocultures with endothelial cells and fibroblasts on micropatterned fibrous mats to promote liver-specific functions and capillary formation capabilities.

    abstract::The maintenance of hepatocyte phenotype and functions remains as a great challenge in the generation of functional liver tissue and in vitro model for drug metabolism studies. The use of hepatocyte coculture systems plays essential roles in the establishment of cell-cell and cell-extracellular matrix communications si...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm401926k

    authors: Liu Y,Li H,Yan S,Wei J,Li X

    更新日期:2014-03-10 00:00:00

  • Dielectric relaxation spectroscopy of hydrated and dehydrated silk fibroin cast from aqueous solution.

    abstract::The dynamics of silk protein in the presence and absence of water has been investigated by dielectric relaxation spectroscopy (DRS). The silk fibroin film cast from its water solution contains 4-7 wt % bound water molecules, which can be removed by dehydration at 165 °C. Temperature and frequency scans were performed ...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm1008316

    authors: Yu L,Hu X,Kaplan D,Cebe P

    更新日期:2010-10-11 00:00:00

  • Short- and Long-Term Effects of X-ray Synchrotron Radiation on Cotton Paper.

    abstract::X-ray analytical techniques are increasingly being used to study manuscripts and works of art on paper, whether with laboratory equipment or synchrotron sources. However, it is difficult to anticipate the impact of X-ray photons on paper- and cellulose-based artifacts, particularly due to the large variety of their co...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/acs.biomac.0c00512

    authors: Gimat A,Schöder S,Thoury M,Missori M,Paris-Lacombe S,Dupont AL

    更新日期:2020-07-13 00:00:00

  • In depth study of a new highly efficient raw starch hydrolyzing α-amylase from Rhizomucor sp.

    abstract::A new α-amylase from Rhizomucor sp. (RA) was studied in detail due to its very efficient hydrolysis of raw starch granules at low temperature (32 °C). RA contains a starch binding domain (SBD) connected to the core amylase catalytic domain by a O-glycosylated linker. The mode of degradation of native maize starch gran...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm100913z

    authors: Tawil G,Viksø-Nielsen A,Rolland-Sabaté A,Colonna P,Buléon A

    更新日期:2011-01-10 00:00:00

  • Comprehensive multidetector HPSEC study on solution properties of cereal arabinoxylans in aqueous and DMSO solutions.

    abstract::The water-soluble arabinoxylans from wheat flour (high, medium, and low viscosity samples) and rye flour (high viscosity sample) were characterized by (1)H NMR spectroscopy and HPSEC with refractive index, light scattering, and viscometric detectors. These cereal arabinoxylans have recently been used as model arabinox...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm9003767

    authors: Pitkänen L,Virkki L,Tenkanen M,Tuomainen P

    更新日期:2009-07-13 00:00:00

  • Decomposable hollow biopolymer-based capsules.

    abstract::Decomposable hollow capsules based on deoxyribonucleic acid (DNA) and a low molecular weight organic molecule, a naturally occurring polyamine, spermidine (SP), were formed by applying the layer-by-layer adsorption strategy to colloid particles, viz., assembling DNA/SP multilayers on colloids and subsequently removing...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm010052w

    authors: Schüler C,Caruso F

    更新日期:2001-10-01 00:00:00

  • Specific Drug Formulation Additives: Revealing the Impact of Architecture and Block Length Ratio.

    abstract::Combining poly(ethylene glycol) (PEG) with sequence-defined peptides in PEG-peptide conjugates offers opportunities to realize next-generation drug formulation additives for overcoming undesired pharmacological profiles of difficult small molecule drugs. The tailored peptide segments provide sequence-specific, noncova...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/acs.biomac.5b00961

    authors: Wieczorek S,Schwaar T,Senge MO,Börner HG

    更新日期:2015-10-12 00:00:00

  • Hyperbranched polyester-based fluorescent probe for histone deacetylase via aggregation-induced emission.

    abstract::Aberrant expression of histone deacetylases (HDACs) is related to various types of cancer and is associated with increased proliferation of tumor cells. Hence, the detection of HDAC activities is of great significance for medical sciences as well as biological diagnostics. Herein, we report a hyperbranched polyester-b...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm401548u

    authors: Yu C,Wu Y,Zeng F,Li X,Shi J,Wu S

    更新日期:2013-12-09 00:00:00

  • Supramolecular hydrogels from cisplatin-loaded block copolymer nanoparticles and α-cyclodextrins with a stepwise delivery property.

    abstract::A stepwise anticancer drug delivery system based on an injectable supramolecular hydrogel was presented. In this system, poly(ethylene glycol)-b-poly(acrylic acid) (PEG-b-PAA) block copolymer nanoparticles containing cisplatin were released by erosion of the hydrogels and then the cisplatin was released from the nanop...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm100889j

    authors: Zhu W,Li Y,Liu L,Chen Y,Wang C,Xi F

    更新日期:2010-11-08 00:00:00

  • Patterning Three-Dimensional Hydrogel Microenvironments Using Hyperbranched Polyglycerols for Independent Control of Mesh Size and Stiffness.

    abstract::The extracellular matrix is an environment rich with structural, mechanical, and molecular signals that can impact cell biology. Traditional approaches in hydrogel biomaterial design often rely on modifying the concentration of cross-linking groups to adjust mechanical properties. However, this strategy provides limit...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/acs.biomac.7b00118

    authors: Pedron S,Pritchard AM,Vincil GA,Andrade B,Zimmerman SC,Harley BA

    更新日期:2017-04-10 00:00:00

  • 3D-Printing of Structure-Controlled Antigen Nanoparticles for Vaccine Delivery.

    abstract::Targeted delivery of antigens to immune cells using micro/nanocarriers may serve as a therapeutic application for vaccination. However, synthetic carriers have potential drawbacks including cytotoxicity, low encapsulation efficiency of antigen, and lack of a morphological design, which limit the translation of the del...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/acs.biomac.9b01775

    authors: Nishiguchi A,Shima F,Singh S,Akashi M,Moeller M

    更新日期:2020-06-08 00:00:00

  • Collagen-Poly(N-isopropylacrylamide) Hydrogels with Tunable Properties.

    abstract::There is a lack of hydrogel materials whose properties can be tuned at the point of use. Biological hydrogels, such as collagen, gelate at physiological temperatures; however, they are not always ideal as scaffolds because of their low mechanical strength. Their mechanics can be improved through cross-linking and chem...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/acs.biomac.5b01251

    authors: Barnes AL,Genever PG,Rimmer S,Coles MC

    更新日期:2016-03-14 00:00:00

  • Hydrogels for osteochondral repair based on photocrosslinkable carbamate dendrimers.

    abstract::First generation, photocrosslinkable dendrimers consisting of natural metabolites (i.e., succinic acid, glycerol, and beta-alanine) and nonimmunogenic poly(ethylene glycol) (PEG) were synthesized divergently in high yields using ester and carbamate forming reactions. Aqueous solutions of these dendrimers were photocro...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm800658x

    authors: Degoricija L,Bansal PN,Söntjens SH,Joshi NS,Takahashi M,Snyder B,Grinstaff MW

    更新日期:2008-10-01 00:00:00

  • Engineered Polymeric Micelles for Combinational Oxidation Anticancer Therapy through Concurrent HO-1 Inhibition and ROS Generation.

    abstract::Cancer cells have a large amount of ROS (reactive oxygen species) because of disturbed ROS homeostasis. Cancer cells therefore undertake redox adaptation to drive proliferation in tumor environments and even survive during anticancer treatment by upregulating endogenous antioxidants. As one of antioxidant defense syst...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/acs.biomac.8b01802

    authors: Noh J,Jung E,Lee J,Hyun H,Hong S,Lee D

    更新日期:2019-02-11 00:00:00

  • Solid-state NMR analysis of a peptide (Gly-Pro-Gly-Gly-Ala)6-Gly derived from a flagelliform silk sequence of Nephila clavipes.

    abstract::Solid-state NMR is especially useful when the structures of peptides and proteins should be analyzed by taking into account the structural distribution, that is, the distribution of the torsion angle of the individual residue. In this study, two-dimensional spin-diffusion solid-state NMR spectra of 13C-double-labeled ...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm0600522

    authors: Ohgo K,Kawase T,Ashida J,Asakura T

    更新日期:2006-04-01 00:00:00

  • Construction, molecular modeling, and simulation of Mycobacterium tuberculosis cell walls.

    abstract::The mycobacterial cell wall is extraordinarily thick and tight consisting mainly of (1). long chain fatty acids, the mycolic acids, and (2). a unique polysaccharide, arabinogalactan (AG). These two chemical constituents are covalently linked through ester bonds. Minnikin (The Biology of the Mycobacteria; Academic: Lon...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm034514c

    authors: Hong X,Hopfinger AJ

    更新日期:2004-05-01 00:00:00

  • Novel therapeutic agents for bone resorption. Part 1. Synthesis and protonation thermodynamics of poly(amido-amine)s containing bis-phosphonate residues.

    abstract::Two poly(amido-amine)s (oligoPAM and oligoNER) containing bis-phosphonate residues were obtained by a Michael-type polyaddition of pamidronate and neridronate to 1,4-bis(acryloyl)piperazine. The SEC (size-exclusion chromatography) and the MALDI-TOF (matrix assisted laser desorption ionization) analyses were consistent...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm0607630

    authors: Casolaro M,Casolaro I,Spreafico A,Capperucci C,Frediani B,Marcolongo R,Margiotta N,Ostuni R,Mendichi R,Samperi F,Ishii T,Ito Y

    更新日期:2006-12-01 00:00:00

  • Development of a sialic acid-containing hydrogel of poly[N-(2-hydroxypropyl) methacrylamide]: characterization and implantation study.

    abstract::This study describes the preparation and the characterization of poly[ N-(2-hydroxypropyl methacrylamide)] hydrogel with bulk-modified saccharidic portion of ganglioside GM 3 (Neu5Ac-alpha2,3-Gal-beta1,4-Glc). The 3'-sialyllactose is a bioactive epitope recognized by many cell surface receptors on viruses, bacteria, a...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm800234r

    authors: Woerly S,Fort S,Pignot-Paintrand I,Cottet C,Carcenac C,Savasta M

    更新日期:2008-09-01 00:00:00

  • New aspects of the formation of physical hydrogels of chitosan in a hydroalcoholic medium.

    abstract::New aspects concerning the mechanism of formation of chitosan physical hydrogels without any cross-linking agent were studied. The gelation took place during the evaporation of a hydroalcoholic solution of chitosan. We first demonstrated that it was possible to form a physical hydrogel from a hydrochloride form of chi...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/bm050653d

    authors: Boucard N,Viton C,Domard A

    更新日期:2005-11-01 00:00:00

  • Visible Light-Induced Hydrogelation of an Alginate Derivative and Application to Stereolithographic Bioprinting Using a Visible Light Projector and Acid Red.

    abstract::Visible light-induced hydrogelation is attractive for various biomedical applications. In this study, hydrogels of alginate with phenolic hydroxyl groups (Alg-Ph) were obtained by irradiating a solution containing the polymer, ruthenium II trisbipyridyl chloride ([Ru(bpy)3]2+) and sodium persulfate (SPS), with visible...

    journal_title:Biomacromolecules

    pub_type: 杂志文章

    doi:10.1021/acs.biomac.7b01827

    authors: Sakai S,Kamei H,Mori T,Hotta T,Ohi H,Nakahata M,Taya M

    更新日期:2018-02-12 00:00:00