Abstract:
:Many primary sensory cilia exhibit unique architectures that are critical for transduction of specific sensory stimuli. Although basic ciliogenic mechanisms are well described, how complex ciliary structures are generated remains unclear. Seminal work performed several decades ago provided an initial but incomplete description of diverse sensory cilia morphologies in C. elegans. To begin to explore the mechanisms that generate these remarkably complex structures, we have taken advantage of advances in electron microscopy and tomography, and reconstructed three-dimensional structures of fifty of sixty sensory cilia in the C. elegans adult hermaphrodite at high resolution. We characterize novel axonemal microtubule organization patterns, clarify structural features at the ciliary base, describe new aspects of cilia-glia interactions, and identify structures suggesting novel mechanisms of ciliary protein trafficking. This complete ultrastructural description of diverse cilia in C. elegans provides the foundation for investigations into underlying ciliogenic pathways, as well as contributions of defined ciliary structures to specific neuronal functions. DOI: http://dx.doi.org/10.7554/eLife.01948.001.
journal_name
Elifejournal_title
eLifeauthors
Doroquez DB,Berciu C,Anderson JR,Sengupta P,Nicastro Ddoi
10.7554/eLife.01948subject
Has Abstractpub_date
2014-03-25 00:00:00pages
e01948issn
2050-084Xjournal_volume
3pub_type
杂志文章相关文献
eLife文献大全abstract::Trimethylamine-oxide (TMAO) is present in seafood which is considered to be beneficial for health. Deep-water animals accumulate TMAO to protect proteins, such as lactate dehydrogenase (LDH), against hydrostatic pressure stress (HPS). We hypothesized that TMAO exerts beneficial effects on the circulatory system and pr...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57028
更新日期:2020-06-08 00:00:00
abstract::In order to assess the contribution of a central clock in the hypothalamic suprachiasmatic nucleus (SCN) to circadian behavior and the organization of peripheral clocks, we generated forebrain/SCN-specific Bmal1 knockout mice by using floxed Bmal1 and pan-neuronal Cre lines. The forebrain knockout mice showed >90% del...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04617
更新日期:2014-12-19 00:00:00
abstract::Mechanistic understanding of eukaryotic ribosome formation requires a detailed structural knowledge of the numerous assembly intermediates, generated along a complex pathway. Here, we present the structure of a late pre-40S particle at 3.6 Å resolution, revealing in molecular detail how assembly factors regulate the t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30189
更新日期:2017-11-20 00:00:00
abstract::Long noncoding RNAs (lncRNAs) have been shown to act as important cell biological regulators including cell fate decisions but are often ignored in human genetics. Combining differential lncRNA expression during neuronal lineage induction with copy number variation morbidity maps of a cohort of children with autism sp...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.41770
更新日期:2019-01-10 00:00:00
abstract::Striatal dysfunction plays an important role in dystonia, but the striatal cell types that contribute to abnormal movements are poorly defined. We demonstrate that conditional deletion of the DYT1 dystonia protein torsinA in embryonic progenitors of forebrain cholinergic and GABAergic neurons causes dystonic-like twis...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.08352
更新日期:2015-06-08 00:00:00
abstract::The basolateral amygdala (BLA) is critical for associating initially neutral cues with appetitive and aversive stimuli and receives dense neuromodulatory acetylcholine (ACh) projections. We measured BLA ACh signaling and activity of neurons expressing CaMKIIα (a marker for glutamatergic principal cells) in mice during...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57335
更新日期:2020-09-18 00:00:00
abstract::An analysis of worldwide human genetic variation reveals the footprints of ancient changes in genomic mutation processes. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.27605
更新日期:2017-05-17 00:00:00
abstract::Humans and other animals routinely identify and attend to sensory stimuli so as to rapidly acquire rewards or avoid aversive experiences. Emotional arousal, a process mediated by the amygdala, can enhance attention to stimuli in a non-spatial manner. However, amygdala neural activity was recently shown to encode spati...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04478
更新日期:2014-10-30 00:00:00
abstract::The medial subnucleus of the amygdala (MeA) plays a central role in processing sensory cues required for innate behaviors. However, whether there is a link between developmental programs and the emergence of inborn behaviors remains unknown. Our previous studies revealed that the telencephalic preoptic area (POA) embr...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21012
更新日期:2017-03-13 00:00:00
abstract::Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric compon...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.34058
更新日期:2018-05-30 00:00:00
abstract::The vertebral column or spine assembles around the notochord rod which contains a core made of large vacuolated cells. Each vacuolated cell possesses a single fluid-filled vacuole, and loss or fragmentation of these vacuoles in zebrafish leads to spine kinking. Here, we identified a mutation in the kinase gene dstyk tha...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51221
更新日期:2020-01-29 00:00:00
abstract::The gut microbiota of patients recently diagnosed with rheumatoid arthritis is enriched in microbes belonging to the Prevotella genus. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.01608
更新日期:2013-11-05 00:00:00
abstract::We recently reported that the C2AB portion of Synaptotagmin 1 (Syt1) could self-assemble into Ca(2+)-sensitive ring-like oligomers on membranes, which could potentially regulate neurotransmitter release. Here we report that analogous ring-like oligomers assemble from the C2AB domains of other Syt isoforms (Syt2, Syt7,...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17262
更新日期:2016-07-19 00:00:00
abstract::Most evolutionary thinking is based on the notion of fitness and related ideas such as fitness landscapes and evolutionary optima. Nevertheless, it is often unclear what fitness actually is, and its meaning often depends on the context. Here we argue that fitness should not be a basal ingredient in verbal or mathemati...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23804
更新日期:2017-02-15 00:00:00
abstract::Smoothened (Smo) inhibition by Patched (Ptch) is central to Hedgehog (Hh) signaling. Ptch, a proton driven antiporter, is required for Smo inhibition via an unknown mechanism. Hh ligand binding to Ptch reverses this inhibition and activated Smo initiates the Hh response. To determine whether Ptch inhibits Smo strictly...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17634
更新日期:2016-08-23 00:00:00
abstract::Humans employ different strategies when making decisions. Previous research has reported reduced reliance on model-based strategies with aging, but it remains unclear whether this is due to cognitive or motivational factors. Moreover, it is not clear how aging affects the metacontrol of decision making, that is the dy...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49154
更新日期:2019-08-09 00:00:00
abstract::Plasma Membrane is the primary structure for adjusting to ever changing conditions. PM sub-compartmentalization in domains is thought to orchestrate signaling. Yet, mechanisms governing membrane organization are mostly uncharacterized. The plant-specific REMORINs are proteins regulating hormonal crosstalk and host inv...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.26404
更新日期:2017-07-31 00:00:00
abstract::The membrane-bound transcription factor ATF6α plays a cytoprotective role in the unfolded protein response (UPR), required for cells to survive ER stress. Activation of ATF6α promotes cell survival in cancer models. We used cell-based screens to discover and develop Ceapins, a class of pyrazole amides, that block ATF6...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11878
更新日期:2016-07-20 00:00:00
abstract::The twin-arginine protein translocation system (Tat) transports folded proteins across the bacterial cytoplasmic membrane and the thylakoid membranes of plant chloroplasts. The Tat transporter is assembled from multiple copies of the membrane proteins TatA, TatB, and TatC. We combine sequence co-evolution analysis, mo...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20718
更新日期:2016-12-03 00:00:00
abstract::Group B Streptococcus (GBS) is the leading cause of invasive bacterial neonatal infections. Late-onset diseases (LOD) occur between 7 and 89 days of life and are largely due to the CC17 GBS hypervirulent clone. We studied the impact of estradiol (E2) and progesterone (P4), which impregnate the fetus during pregnancy, ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.48772
更新日期:2019-11-11 00:00:00
abstract::The factors that govern assembly of the gut microbiota are insufficiently understood. Here, we test the hypothesis that inter-individual microbiota variation can arise solely from differences in the order and timing by which the gut is colonized early in life. Experiments in which mice were inoculated in sequence eith...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.36521
更新日期:2018-09-18 00:00:00
abstract::To initiate DNA replication, cells first load an MCM helicase double hexamer at origins in a reaction requiring ORC, Cdc6, and Cdt1, also called pre-replicative complex (pre-RC) assembly. The essential mechanistic role of Cdc6 ATP hydrolysis in this reaction is still incompletely understood. Here, we show that althoug...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.05795
更新日期:2015-08-25 00:00:00
abstract::SynGAP is a postsynaptic density (PSD) protein that binds to PDZ domains of the scaffold protein PSD-95. We previously reported that heterozygous deletion of Syngap1 in mice is correlated with increased steady-state levels of other key PSD proteins that bind PSD-95, although the level of PSD-95 remains constant (Walku...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52656
更新日期:2020-01-15 00:00:00
abstract::While host immune receptors detect pathogen-associated molecular patterns to activate immunity, pathogens attempt to deregulate host immunity through secreted effectors. Fungi employ LysM effectors to prevent recognition of cell wall-derived chitin by host immune receptors, although the mechanism to compete for chitin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00790
更新日期:2013-07-02 00:00:00
abstract::Although individuals of many species inexorably age, a number of observations established that the rate of aging is modulated in response to a variety of mild stresses. Here, we investigated how heat stress promotes longevity in yeast. We show that upon growth at higher temperature, yeast cells relax the retention of ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.28329
更新日期:2017-12-28 00:00:00
abstract::The transcription factor TCF7L1 is an embryonic stem cell signature gene that is upregulated in multiple aggressive cancer types, but its role in skin tumorigenesis has not yet been defined. Here we document TCF7L1 upregulation in skin squamous cell carcinoma (SCC) and demonstrate that TCF7L1 overexpression increases ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23242
更新日期:2017-05-03 00:00:00
abstract::Viral replicase recruitment and long-range RNA interactions are essential for RNA virus replication, yet the mechanism of their interplay remains elusive. Flaviviruses include numerous important human pathogens, e.g., dengue virus (DENV) and Zika virus (ZIKV). Here, we revealed a highly conserved, conformation-tunable...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17636
更新日期:2016-10-01 00:00:00
abstract::Yes. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.40018
更新日期:2018-07-23 00:00:00
abstract::The representation of position in the mammalian brain is distributed across multiple neural populations. Grid cell modules in the medial entorhinal cortex (MEC) express activity patterns that span a low-dimensional manifold which remains stable across different environments. In contrast, the activity patterns of hippo...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56894
更新日期:2020-08-11 00:00:00
abstract::Neural circuits that control copulation in male flies have been identified. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.22215
更新日期:2016-11-15 00:00:00