Cryo-EM structure of a late pre-40S ribosomal subunit from Saccharomyces cerevisiae.

abstract：:KdpFABC is an ATP-dependent K+ pump that ensures bacterial survival in K+-deficient environments. Whereas transcriptional activation of kdpFABC expression is well studied, a mechanism for down-regulation when K+ levels are restored has not been described. Here, we show that KdpFABC is inhibited when cells return to a ...

journal_title：eLife

authors： Sweet ME,Zhang X,Erdjument-Bromage H,Dubey V,Khandelia H,Neubert TA,Pedersen BP,Stokes DL

更新日期：2020-09-21 00:00:00

abstract：:Asymmetric disassembly of the synaptonemal complex (SC) is crucial for proper meiotic chromosome segregation. However, the signaling mechanisms that directly regulate this process are poorly understood. Here we show that the mammalian Rho GEF homolog, ECT-2, functions through the conserved RAS/ERK MAP kinase signaling...

journal_title：eLife

authors： Nadarajan S,Mohideen F,Tzur YB,Ferrandiz N,Crawley O,Montoya A,Faull P,Snijders AP,Cutillas PR,Jambhekar A,Blower MD,Martinez-Perez E,Harper JW,Colaiacovo MP

更新日期：2016-02-27 00:00:00

abstract：:Clinical trials are currently underway to assess the efficacy of forniceal deep brain stimulation (DBS) for improvement of memory in Alzheimer's patients, and forniceal DBS has been shown to improve learning and memory in a mouse model of Rett syndrome (RTT), an intellectual disability disorder caused by loss-of-funct...

journal_title：eLife

authors： Pohodich AE,Yalamanchili H,Raman AT,Wan YW,Gundry M,Hao S,Jin H,Tang J,Liu Z,Zoghbi HY

更新日期：2018-03-23 00:00:00

abstract：:Myelin destruction is followed by resident glia activation and mobilization of endogenous progenitors (OPC) which participate in myelin repair. Here we show that in response to demyelination, mature oligodendrocytes (OLG) bordering the lesion express Ndst1, a key enzyme for heparan sulfates (HS) synthesis. Ndst1+ OLG ...

journal_title：eLife

authors： Macchi M,Magalon K,Zimmer C,Peeva E,El Waly B,Brousse B,Jaekel S,Grobe K,Kiefer F,Williams A,Cayre M,Durbec P

更新日期：2020-06-09 00:00:00

abstract：:From bacteria to mammals, different phospholipid species are segregated between the inner and outer leaflets of the plasma membrane by ATP-dependent lipid transporters. Disruption of this asymmetry by ATP-independent phospholipid scrambling is important in cellular signaling, but its mechanism remains incompletely und...

journal_title：eLife

authors： Jiang T,Yu K,Hartzell HC,Tajkhorshid E

更新日期：2017-09-16 00:00:00

abstract：:Antibiotics are widely used in the treatment of bacterial infections. Although known for their microbicidal activity, antibiotics may also interfere with the host's immune system. Here, we analyzed the effects of bedaquiline (BDQ), an inhibitor of the mycobacterial ATP synthase, on human macrophages. Genome-wide gene ...

journal_title：eLife

authors： Giraud-Gatineau A,Coya JM,Maure A,Biton A,Thomson M,Bernard EM,Marrec J,Gutierrez MG,Larrouy-Maumus G,Brosch R,Gicquel B,Tailleux L

更新日期：2020-05-04 00:00:00

abstract：:Phenazines are natural bacterial antibiotics that can protect crops from disease. However, for most crops it is unknown which producers and specific phenazines are ecologically relevant, and whether phenazine biodegradation can counter their effects. To better understand their ecology, we developed and environmentally...

journal_title：eLife

authors： Dar D,Thomashow LS,Weller DM,Newman DK

更新日期：2020-09-15 00:00:00

abstract：:Anterograde transport of late endosomes or lysosomes (LE/Lys) is crucial for proper axon growth. However, the role of energetic nutrients has been poorly explored. Malonyl-CoA is a precursor of fatty acids, and its intracellular levels highly fluctuate depending on glucose availability or the energy sensor AMP-activat...

journal_title：eLife

authors： Palomo-Guerrero M,Fadó R,Casas M,Pérez-Montero M,Baena M,Helmer PO,Domínguez JL,Roig A,Serra D,Hayen H,Stenmark H,Raiborg C,Casals N

更新日期：2019-12-23 00:00:00

abstract：:XMAP215/Stu2/Alp14 proteins accelerate microtubule plus-end polymerization by recruiting tubulins via arrays of tumor overexpressed gene (TOG) domains, yet their mechanism remains unknown. Here, we describe the biochemical and structural basis for TOG arrays in recruiting and polymerizing tubulins. Alp14 binds four tu...

journal_title：eLife

authors： Nithianantham S,Cook BD,Beans M,Guo F,Chang F,Al-Bassam J

更新日期：2018-11-13 00:00:00

abstract：:The germ line provides the cellular link between generations of multicellular organisms, its cells entering the meiotic cell cycle only once each generation. However, the mechanisms governing this initiation of meiosis remain poorly understood. Here, we examined cells undergoing meiotic initiation in mice, and we foun...

journal_title：eLife

authors： Kojima ML,de Rooij DG,Page DC

更新日期：2019-02-27 00:00:00

abstract：:How does the skeleton detect and adapt to changes in the mechanical load it has to carry? ...

journal_title：eLife

authors： Haelterman N,Lim J

更新日期：2019-10-07 00:00:00

abstract：:The cellular architecture of the ventral tegmental area (VTA), the main hub of the brain reward system, remains only partially characterized. To extend the characterization to inhibitory neurons, we have identified three distinct subtypes of somatostatin (Sst)-expressing neurons in the mouse VTA. These neurons differ ...

journal_title：eLife

authors： Nagaeva E,Zubarev I,Bengtsson Gonzales C,Forss M,Nikouei K,de Miguel E,Elsilä L,Linden AM,Hjerling-Leffler J,Augustine GJ,Korpi ER

更新日期：2020-08-04 00:00:00

abstract：:Site-specific histone ubiquitylation plays a central role in orchestrating the response to DNA double-strand breaks (DSBs). DSBs elicit a cascade of events controlled by the ubiquitin ligase RNF168, which promotes the accumulation of repair factors such as 53BP1 and BRCA1 on the chromatin flanking the break site. RNF1...

journal_title：eLife

authors： Kitevski-LeBlanc J,Fradet-Turcotte A,Kukic P,Wilson MD,Portella G,Yuwen T,Panier S,Duan S,Canny MD,van Ingen H,Arrowsmith CH,Rubinstein JL,Vendruscolo M,Durocher D,Kay LE

更新日期：2017-04-13 00:00:00

abstract：:Ki-67 and RepoMan have key roles during mitotic exit. Previously, we showed that Ki-67 organizes the mitotic chromosome periphery and recruits protein phosphatase 1 (PP1) to chromatin at anaphase onset, in a similar manner as RepoMan (Booth et al., 2014). Here we show how Ki-67 and RepoMan form mitotic exit phosphatas...

journal_title：eLife

authors： Kumar GS,Gokhan E,De Munter S,Bollen M,Vagnarelli P,Peti W,Page R

更新日期：2016-08-30 00:00:00

abstract：:The numerous neurons and glia that form the brain originate from tightly controlled growth and division of neural stem cells, regulated systemically by important known stem cell-extrinsic signals. However, the cell-intrinsic mechanisms that control the distinctive proliferation rates of individual neural stem cells ar...

journal_title：eLife

authors： Samuels TJ,Järvelin AI,Ish-Horowicz D,Davis I

更新日期：2020-01-14 00:00:00

abstract：:Adult neurogenesis in the hippocampus may represent a form of plasticity in brain functions including mood, learning and memory. However, mechanisms underlying neural stem/progenitor cells (NSPCs) proliferation are not well understood. We found that Agrin, a factor critical for neuromuscular junction formation, is ele...

journal_title：eLife

authors： Zhang H,Sathyamurthy A,Liu F,Li L,Zhang L,Dong Z,Cui W,Sun X,Zhao K,Wang H,Ho HH,Xiong WC,Mei L

更新日期：2019-07-03 00:00:00

abstract：:A key hallmark of visual perceptual awareness is robustness to instabilities arising from unnoticeable eye and eyelid movements. In previous human intracranial (iEEG) work (Golan et al., 2016) we found that excitatory broadband high-frequency activity transients, driven by eye blinks, are suppressed in higher-level bu...

journal_title：eLife

authors： Golan T,Davidesco I,Meshulam M,Groppe DM,Mégevand P,Yeagle EM,Goldfinger MS,Harel M,Melloni L,Schroeder CE,Deouell LY,Mehta AD,Malach R

更新日期：2017-08-29 00:00:00

abstract：:The enteric nervous system controls a variety of gastrointestinal functions including intestinal motility. The minimal neuronal circuit necessary to direct peristalsis is well-characterized but several intestinal regions display also other motility patterns for which the underlying circuits and connectivity schemes th...

journal_title：eLife

authors： Li Z,Hao MM,Van den Haute C,Baekelandt V,Boesmans W,Vanden Berghe P

更新日期：2019-02-12 00:00:00

abstract：:A pair of neurons is required for nematodes to be able to navigate using the Earth's magnetic field. ...

journal_title：eLife

authors： Rankin CH,Lin CH

更新日期：2015-08-05 00:00:00

abstract：:How producers of public goods persist in microbial communities is a major question in evolutionary biology. Cooperation is evolutionarily unstable, since cheating strains can reproduce quicker and take over. Spatial structure has been shown to be a robust mechanism for the evolution of cooperation. Here we study how s...

journal_title：eLife

authors： Uppal G,Vural DC

更新日期：2018-05-22 00:00:00

abstract：:Plasma Membrane is the primary structure for adjusting to ever changing conditions. PM sub-compartmentalization in domains is thought to orchestrate signaling. Yet, mechanisms governing membrane organization are mostly uncharacterized. The plant-specific REMORINs are proteins regulating hormonal crosstalk and host inv...

journal_title：eLife

authors： Gronnier J,Crowet JM,Habenstein B,Nasir MN,Bayle V,Hosy E,Platre MP,Gouguet P,Raffaele S,Martinez D,Grelard A,Loquet A,Simon-Plas F,Gerbeau-Pissot P,Der C,Bayer EM,Jaillais Y,Deleu M,Germain V,Lins L,Mongrand S

更新日期：2017-07-31 00:00:00

abstract：:Transport of biologically active molecules across tight epithelial barriers is a major challenge preventing therapeutic peptides from oral drug delivery. Here, we identify a set of synthetic glycosphingolipids that harness the endogenous process of intracellular lipid-sorting to enable mucosal absorption of the incret...

journal_title：eLife

authors： Garcia-Castillo MD,Chinnapen DJ,Te Welscher YM,Gonzalez RJ,Softic S,Pacheco M,Mrsny RJ,Kahn CR,von Andrian UH,Lau J,Pentelute BL,Lencer WI

更新日期：2018-05-31 00:00:00

abstract：:Heterochromatin formed by the SUV39 histone methyltransferases represses transcription from repetitive DNA sequences and ensures genomic stability. How SUV39 enzymes localize to their target genomic loci remains unclear. Here, we demonstrate that chromatin-associated RNA contributes to the stable association of SUV39H...

journal_title：eLife

authors： Johnson WL,Yewdell WT,Bell JC,McNulty SM,Duda Z,O'Neill RJ,Sullivan BA,Straight AF

更新日期：2017-08-01 00:00:00

abstract：:Neuroligins (NLGNs) are postsynaptic cell adhesion molecules that interact trans-synaptically with neurexins to mediate synapse development and function. NLGN2 is only at inhibitory synapses while NLGN3 is at both excitatory and inhibitory synapses. We found that NLGN3 function at inhibitory synapses in rat CA1 depend...

journal_title：eLife

authors： Nguyen QA,Horn ME,Nicoll RA

更新日期：2016-11-02 00:00:00

abstract：:Dorsal/ventral (DV) patterning of the sea urchin embryo relies on a ventrally-localized organizer expressing Nodal, a pivotal regulator of the DV gene regulatory network. However, the inceptive mechanisms imposing the symmetry-breaking are incompletely understood. In Paracentrotus lividus, the Hbox12 homeodomain-conta...

journal_title：eLife

authors： Cavalieri V,Spinelli G

更新日期：2014-12-02 00:00:00

abstract：:Antagonistic receptive field surrounds are a near-universal property of early sensory processing. A key assumption in many models for retinal ganglion cell encoding is that receptive field surrounds are added only to the fully formed center signal. But anatomical and functional observations indicate that surrounds are...

journal_title：eLife

authors： Turner MH,Schwartz GW,Rieke F

更新日期：2018-09-06 00:00:00

abstract：:Chronic endoplasmic reticulum (ER) stress results in toxicity that contributes to multiple human disorders. We report a stress resolution pathway initiated by the nuclear receptor LRH-1 that is independent of known unfolded protein response (UPR) pathways. Like mice lacking primary UPR components, hepatic Lrh-1-null m...

journal_title：eLife

authors： Mamrosh JL,Lee JM,Wagner M,Stambrook PJ,Whitby RJ,Sifers RN,Wu SP,Tsai MJ,Demayo FJ,Moore DD

更新日期：2014-04-15 00:00:00

abstract：:As the staff of the MRC Laboratory of Molecular Biology settle into their new building in Cambridge, its director Hugh Pelham explains the challenges of living up to its prestigious past. ...

journal_title：eLife

authors： Pelham H

更新日期：2013-05-28 00:00:00

abstract：:The new open-access journal eLife has launched, making its first content available in PubMed Central. In addition to publishing science of the highest quality, the journal aims to improve both the peer-review process and the presentation of new research results. ...

journal_title：eLife

authors： Schekman R,Patterson M,Watt F,Weigel D

更新日期：2012-10-15 00:00:00

abstract：:Cell division is essential to expand, shape, and replenish epithelia. In the adult small intestine, cells from a common progenitor intermix with other lineages, whereas cell progeny in many other epithelia form contiguous patches. The mechanisms that generate these distinct patterns of progeny are poorly understood. U...

journal_title：eLife

authors： McKinley KL,Stuurman N,Royer LA,Schartner C,Castillo-Azofeifa D,Delling M,Klein OD,Vale RD

更新日期：2018-06-13 00:00:00