Abstract:
:Despite advances in detection and treatment for breast cancer (BC), recurrence and death rates remain unacceptably high. Therefore, more convenient diagnostic and prognostic methods still required to optimize treatments among the patients. Here, we report the clinical significance of the serum cathepsin E (CatE) activity as a novel prognostic marker for BC. Correlation analysis between the serum levels of CatE expression and clinicopathological parameters revealed that the activity levels, but not the protein levels, were negatively associated with the stages and progression of BC. Univariate and multivariate analyses demonstrated that the serum CatE activity was significantly correlated with favorable prognostic outcomes of the patients. The functional link of CatE expression to BC progression was further corroborated by in vivo and in vitro studies with mice exhibiting different levels of CatE expression. Multiparous CatE (-) (/) (-) mice spontaneously developed mammary tumors concomitant with morphological transformation and altered growth characteristics of the mammary glands. These alterations were associated in part with the induction of epithelial-mesenchymal transition and the activation of β-catenin-dependent pathway in mammary cells. Loss of CatE strongly induced the translocation and accumulation of Wnt5a in the nuclei, thereby leading to the aberrant trafficking, maturation and secretion of Wnt5a and the impaired signaling. The interaction of CatE and Wnt5a was verified by proximity ligation assay and by knockdown or restoration of CatE expression in the mammary cells. Consequently, our data demonstrate that CatE contributes to normal growth and development of mammary glands through proper trafficking and secretion of Wnt5a.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Kawakubo T,Yasukochi A,Toyama T,Takahashi S,Okamoto K,Tsukuba T,Nakamura S,Ozaki Y,Nishigaki K,Yamashita H,Yamamoto Kdoi
10.1093/carcin/bgt373subject
Has Abstractpub_date
2014-03-01 00:00:00pages
714-26issue
3eissn
0143-3334issn
1460-2180pii
bgt373journal_volume
35pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Epithelial cells derived from 46 human breast tissue samples of patients suffering from breast cancer have been cultivated. Twenty-five of these cell cultures stemmed from normal and 21 from tumor tissues. Moderate to large variations of protein levels of three protein kinase C (PKC) isoenzymes (alpha, delta and epsil...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.2.359
更新日期:1994-02-01 00:00:00
abstract::Human papillomavirus (HPV) type 16 infection is an etiologic factor in a subset of head and neck squamous cell carcinomas (HNSCC). It is unknown if host genetic susceptibility modifies the HPV16-HNSCC association. DNA samples collected as part of a Boston area case-control study of HNSCC were genotyped for single-nucl...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp076
更新日期:2009-06-01 00:00:00
abstract::Long non-coding RNAs (lncRNAs) are regarded as important functional regulators of various biological processes and are also known to be involved in the occurrence and development of human cancers, including breast cancer (BC). In our present study, the RNA expression profiling data for a large cohort of human BC sampl...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgz197
更新日期:2020-09-24 00:00:00
abstract::The purpose of this study was to compare the metabolism and DNA binding of aflatoxicol (AFL) with that of aflatoxin B1 (AFB1) in vivo and in isolated hepatocytes from Mt Shasta strain rainbow trout (Salmo gairdneri). Maximum total binding of [3H]AFL to liver DNA from trout exposed by intraperitoneal injection was 38-4...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.8.1065
更新日期:1987-08-01 00:00:00
abstract::Women who carry the BRCA mutation are at high lifetime risk of breast cancer, but there is no consensus regarding an effective and safe chemoprevention strategy. A large body of evidence suggests that 3,3-diindolylmethane (DIM), a dimer of indole-3-carbinol found in cruciferous vegetables, can potentially prevent carc...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgaa050
更新日期:2020-10-15 00:00:00
abstract::Histone deacetylase (HDAC) inhibitors have the potential to derepress epigenetically silenced genes in cancer cells, leading to cell cycle arrest and apoptosis. In the present study, we screened several garlic-derived small organosulfur compounds for their ability to inhibit HDAC activity in vitro. Among the organosul...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn165
更新日期:2008-09-01 00:00:00
abstract::Purified DNA was modified in vitro by 3H-labelled O-acetyl or O,O'-diacetyl-4-hydroxyaminoquinoline-1-oxide (Ac4HAQO or di Ac-4HAQO). It was then subjected to the action of the single-stranded DNA specific nuclease S1 and the digested fractions were analysed. For both types of modified DNA, the release of non-modified...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.4.435
更新日期:1982-01-01 00:00:00
abstract::The risk of squamous cell cancers of the oral cavity (OSCC) is strongly related to the use of tobacco and alcohol. N-Acetyl transferases 1 and 2 (NAT2) metabolize aryl- and heterocyclic amines that are present in tobacco smoke. NAT2 slow acetylator phenotype or genotype is related to reduced ability to detoxify these ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/22.12.1993
更新日期:2001-12-01 00:00:00
abstract::The effect of daily parenteral administration of a long-acting analogue of somatostatin (SMS 201-995) on the development of intestinal tumours and the rate of crypt cell proliferation in azoxymethane-treated rats has been studied. SMS 201-995 had no significant effect on the number of colonic tumours induced. In the d...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.4.561
更新日期:1987-04-01 00:00:00
abstract::The measurement of oxidative damage to cellular DNA is a challenging analytical problem requiring highly sensitive and specific methods. In addition, artefactual DNA oxidation during its extraction and subsequent work-up may give rise to overestimated levels of oxidized DNA bases. In the present study, we have used (1...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.11.1911
更新日期:2002-11-01 00:00:00
abstract::Reflux of gastroduodenal contents and consequent inflammatory responses are associated with the development of Barrett's oesophagus (BO) and the promotion of oesophageal adenocarcinoma (OAC). Deregulation of inflammatory processes is a hallmark of oesophageal cancer. In this study, we aimed to investigate (i) the tran...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq036
更新日期:2010-05-01 00:00:00
abstract::The effect of exposing mice to both a chemical carcinogen and leukemia virus with and without an inhibitor of DNA repair were compared. The data indicated that benzo[a]pyrene (BP) could exert a potentiating effect of Friend viral leukemogenesis in mice, which was dependent on the relative times of administration of th...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/2.1.1
更新日期:1981-01-01 00:00:00
abstract::Gene-environment interactions play an important role in folate metabolism, with a potential impact on human health. Deficiencies in the uptake of key micronutrients and variant genotypes can affect the folic acid cycle, modulating methyl group transfer in key processes and leading to increased cancer risk and Down syn...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgg064
更新日期:2003-06-01 00:00:00
abstract::TMPRSS4 is a novel type II transmembrane serine protease that is highly expressed on the cell surface in pancreatic, thyroid and other cancer tissues, although its oncogenic significance and molecular mechanisms are unknown. Previously, we have shown that TMPRSS4 promotes invasion, migration and metastasis of human tu...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq024
更新日期:2010-04-01 00:00:00
abstract::Inhalation of diesel exhaust (DE), which contains soot particles with adsorbed mutagenic organic compounds, and its virtually mutagen-free soot particle analog, carbon black (CB), produce similar types and prevalences of pulmonary neoplasms in chronically exposed F344 rats. This result suggests that DE-induced neoplas...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.5.1215
更新日期:1995-05-01 00:00:00
abstract::N2-ethylidene-2'-deoxyguanosine (N2-ethylidene-dG) is a major DNA adduct induced by acetaldehyde. Although it is unstable in the nucleoside form, it is relatively stable when present in DNA. In this study, we analyzed three acetaldehyde-derived DNA adducts, N2-ethylidene-dG, N2-ethyl-2'-deoxyguanosine (N2-Et-dG) and a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm057
更新日期:2007-11-01 00:00:00
abstract::Sequencing of p53 exons 5-8 was carried out on 51 initial superficial bladder tumors selected on the basis of high grade and/or p53 overexpression (immunohistochemistry without antigen retrieval). Fourteen point mutations in 13 tumors and one 21 bp deletion in another tumor were identified. In addition, a germ-line mu...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.1.101
更新日期:2000-01-01 00:00:00
abstract::We examined the effects of the aliphatic amides isopropyl-valeramide (IVA) and allylisopropylacetamide (AIA) on oncogenic transformation and sister chromatid exchanges (SCE) induced by cyclopenta[cd]pyrene (CPP) and benzo[a]pyrene (B[a]P) in C3H/10T1/2 cells and on B[a]Pdiol-epoxide (BPDE)-induced mutation at the HGPR...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.10.1647
更新日期:1986-10-01 00:00:00
abstract::The role of rat O6-alkylguanine-DNA alkyltransferase (AGT) in modulating mutagenesis by O6-substituted guanines in the rat H-ras gene was examined. Rat4 cells were transfected with vectors carrying O6-methyl-, O6-ethyl- or O6-benzylguanine residues in place of the normal guanines at either the first, second, or both t...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.4.593
更新日期:1993-04-01 00:00:00
abstract::Recently, TWIST, a basic helix-loop-helix transcription factor, is suggested to be an oncogene because of its over-expression in many types of human cancer and its positive role in promoting cell survival. The aim of this study was to investigate the role of TWIST on the growth of human epithelial cells. Using two imm...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm185
更新日期:2007-12-01 00:00:00
abstract::In 2000, Douglas Hanahan and Robert Weinberg published a review detailing the six hallmarks of cancer. These are six phenotypes that a tumour requires in order to become a fully fledged malignancy: persistent growth signals, evasion of apoptosis, insensitivity to anti-growth signals, unlimited replicative potential, a...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgp070
更新日期:2009-08-01 00:00:00
abstract::The antitumorigenic activities of polyphenols such as ellagitannins and anthocyanins in pomegranate (Punica granatum L.) have been previously studied where cytotoxic, anti-inflammatory and antioxidant effects were evident in various cancer models. The objective of this study was to investigate the role of miR-126/vasc...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt295
更新日期:2013-12-01 00:00:00
abstract::Our previous report demonstrated that RSK2 plays an important role in cell proliferation and transformation induced by tumor promoters such as epidermal growth factor mediated through the N-terminal kinase domain of RSK2 in JB6 Cl41 mouse skin epidermal cells in vitro. However, no direct evidence has been reported reg...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs271
更新日期:2012-12-01 00:00:00
abstract::A plant polysaccharide, Aloe gel extract, was reported to have an inhibitory effect on benzo[a]pyrene (B[a]P)-DNA adduct formation in vitro and in vivo. Hence, chemopreventive effects of plant polysaccharides [Aloe barbadensis Miller (APS), Lentinus edodes (LPS), Ganoderma lucidum (GPS) and Coriolus versicolor (CPS)] ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.8.1637
更新日期:1999-08-01 00:00:00
abstract::Insulin-like growth factor 1 receptor (IGF-1R) activation is required for prostate cell proliferation. Prostate cancer is one of the most commonly diagnosed malignant tumors in Western countries. Overexpression of IGF-1R in prostate cancer is associated with tumor growth. These suggest that IGF-1R inhibitory agents ma...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgl189
更新日期:2007-03-01 00:00:00
abstract::Oncogenic pathogens can disturb tissue homeostasis and initiate immune responses for oncogenicity clearance and homeostasis restoration, while failed clearance and chronic inflammation may result in tumorigenesis. The primary tumor development will undergo a cancer immunoediting process, including three phases, termed...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgaa058
更新日期:2020-06-12 00:00:00
abstract::It has recently been reported that the XPD (ERCC2) gene is an integral component of the basal transcription factor TFIIH. We have studied the direct role of this repair gene on the fine structure of DNA repair in hamster cells. The gene and strand specific DNA repair of UV induced pyrimidine dimers was determined in w...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.4.681
更新日期:1997-04-01 00:00:00
abstract::Epithelial-mesenchymal transition (EMT) is an important process implicated in tumor invasion and metastasis. Twist1 is a transcription factor that induces EMT, including E-cadherin suppression and cancer cell migration and invasion; hence it promotes cancer metastasis. Twist1 directly or indirectly regulates the expre...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgv005
更新日期:2015-03-01 00:00:00
abstract::Glycidyl ethers are reactive epoxides used as components of a variety of epoxy materials. These compounds are known to cause allergic reactions, but since they are generally also genotoxic it would be of interest to evaluate the risk for induction of such effects. Reaction products of allyl glycidyl ether with nucleic...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.7.1465
更新日期:1996-07-01 00:00:00
abstract::2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a mammary gland carcinogen present in the human diet. Herein, the expression of estrogen receptor alpha (ERalpha), estrogen receptor beta (ER beta) and progesterone receptor (PR) was examined in mammary gland carcinomas induced by PhIP in female Sprague-Dawley ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi013
更新日期:2005-04-01 00:00:00