Abstract:
:The antitumorigenic activities of polyphenols such as ellagitannins and anthocyanins in pomegranate (Punica granatum L.) have been previously studied where cytotoxic, anti-inflammatory and antioxidant effects were evident in various cancer models. The objective of this study was to investigate the role of miR-126/vascular cell adhesion molecule 1 (VCAM-1) and miR-126/phosphatidylinositol-3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) in pomegranate-mediated anti-inflammatory and anticarcinogenic effects in vivo and in vitro. Sprague-Dawley rats (n = 10 per group) received pomegranate juice (2504.74 mg gallic acid equivalents/l) or a polyphenol-free control beverage ad libitum for 10 weeks and were injected with azoxymethane (AOM) subcutaneously (15mg/kg) at weeks 2 and 3. Consumption of pomegranate juice suppressed the number of aberrant crypt foci (ACF) and dysplastic ACF by 29 and 53.5% (P = 0.05 and 0.04), respectively, and significantly lowered proliferation of mucosa cells. Pomegranate juice significantly downregulated proinflammatory enzymes nitric oxide synthase and cyclooxygenase-2 messenger RNA (mRNA) and protein expression. In addition, it suppressed nuclear factor-κB and VCAM-1 mRNA and protein expression in AOM-treated rats. Pomegranate also inhibited phosphorylation of PI3K/AKT and mTOR expression and increased the expression of miR-126. The specific target and functions of miR-126 were investigated in HT-29 colon cancer cell lines. In vitro, the involvement of miR-126 was confirmed using the antagomiR for miR-126, where pomegranate reversed the effects of the antagomiR on the expression of miR-126, VCAM-1 and PI3K p85β. In summary, therapeutic potentials of pomegranate in colon tumorigenesis were due in part to targeting miR-126-regulated pathways, which contributes in the underlying anti-inflammatory mechanisms.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Banerjee N,Kim H,Talcott S,Mertens-Talcott Sdoi
10.1093/carcin/bgt295subject
Has Abstractpub_date
2013-12-01 00:00:00pages
2814-22issue
12eissn
0143-3334issn
1460-2180pii
bgt295journal_volume
34pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::The 32P-postlabeling method has been adapted for the analysis of thymidine-cis-glycol-3'-phosphate (cis-dTGp,cis-5,6-dihydroxy-5,6-dihydrothymidine-3'-phosphat e). Cis-dTGp was isolated and purified from normal nucleotides by phenylboronate affinity chromatography and phosphorylated by T4 polynucleotide kinase in pres...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.1.43
更新日期:1989-01-01 00:00:00
abstract::The phylogenetically distant F344 and BN rat strains and their (BN x F344) F1 hybrids were compared for susceptibility to hepatocarcinogenesis using the 'resistant hepatocyte' model. Quantitative stereological analysis of frequency (number/liver) and size (mean volume and volume fraction) of placental form glutathione...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.8.1765
更新日期:1996-08-01 00:00:00
abstract::The Bowman-Birk protease inhibitor (BBI) is a legume-derived inhibitor of chymotrypsin and trypsin that has been shown to suppress cellular transformation and tumorigenesis. In the present investigation the effects of various BBI administration schedules were evaluated for suppression of 3-methylcholanthrene (3-MCA)-i...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.5.935
更新日期:1991-05-01 00:00:00
abstract::17beta-estradiol (E2), estrone and diethylstilbestrol (DES) had no effect on nuclear and nucleolar RNA synthesis in vitro. However, after reacting with dimethyldioxirane (DMDO), a versatile epoxide-forming oxidant, these estrogens were able to inhibit and in a dose-dependent manner nuclear and nucleolar RNA synthesis ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.9.1957
更新日期:1996-09-01 00:00:00
abstract::Co-exposure to cadmium, cobalt, lead and other heavy metals occurs in many occupational settings, such as pigment and batteries production, galvanization and recycling of electric tools. However, little is known about interactions between several heavy metals. In the present study we determined DNA single strand break...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/24.1.63
更新日期:2003-01-01 00:00:00
abstract::The role of parity before and after N-methyl-N-nitrosourea (MNU) treatment in protection against mammary carcinogenesis was investigated. The effect of lactation on reduction in the incidence of mammary carcinoma was also examined. Parous rats were compared with respective age-matched virgins (AMVs). Pregnancy and lac...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.4.623
更新日期:1999-04-01 00:00:00
abstract::MicroRNAs (miRNAs) are small non-coding RNAs that are involved in different biological processes by suppressing target gene expression. Altered expression of miR-30a-5p has been reported in colon carcinoma. To elucidate its potential biological role in colon cancer, miR-30a-5p was overexpressed via a lentiviral vector...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs020
更新日期:2012-04-01 00:00:00
abstract::1,3-Butadiene (BD) is an indirect alkylating agent that has greater cancer potency in the mouse than in the rat. The purpose of the present study was to compare the mutagenic potency of BD at the hprt locus of T-lymphocytes of exposed mice and rats and to determine whether mutations induced in this marker gene can be ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.6.1019
更新日期:1998-06-01 00:00:00
abstract::Abl interactor 1 (Abi1) is a key regulator of actin polymerization/depolymerization. The involvement of Abi1 in the development of abnormal cytoskeletal functions of cancer cells has recently been reported. It remains unclear, however, how Abi1 exerts its effects in tumor cells and whether it contributes to tumor prog...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp251
更新日期:2009-12-01 00:00:00
abstract::2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), the most abundant heterocyclic amine produced during the cooking of meats and fish, is suspected to be a human carcinogen. Metabolic activation of PhIP is primarily mediated by the enzyme cytochrome P450 (CYP) 1A2. Metabolism of PhIP by CYP1A2 differs considerabl...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq235
更新日期:2011-02-01 00:00:00
abstract::The human guanylate-binding protein 1 (GBP-1) is among the proteins the most highly induced by interferon-γ (IFN-γ) in every cell type investigated as yet. In vivo, GBP-1 expression is associated with the presence of inflammation and has been observed in autoimmune diseases, inflammatory bowel diseases (IBD) and cance...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs310
更新日期:2013-01-01 00:00:00
abstract::Observational studies have been largely consistent in showing an inverse association between vitamin D and an individual's risk of developing colorectal cancer. Vitamin D protection is further supported by a range of preclinical colon cancer models, including carcinogen, genetic and dietary models. A large number of m...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgr301
更新日期:2012-03-01 00:00:00
abstract::O6-Alkylguanine-DNA alkyltransferase (AGAT) activity was determined in macroscopically normal peripheral lung tissues from 122 patients undergoing lung surgery. AGAT activity of smokers was 1.4-fold higher than that of lifetime non-smokers (P = 0.030). Less than one year of abstinence from smoking did not cause a sign...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.8.1535
更新日期:1994-08-01 00:00:00
abstract::Supplementation by beta-carotene has unexpectedly appeared to increase lung cancer risk among smokers. In order to explain this it has been suggested that at high serum levels of beta-carotene, prooxidant characteristics of beta-carotene may become manifest, yielding reactive oxygen species (ROS) and inducing oxidativ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh125
更新日期:2004-07-01 00:00:00
abstract::Multidrug resistance 2 (Mdr2), also called adenosine triphosphate-binding cassette B4 (ABCB4), is the transporter of phosphatidylcholine (PC) at the canalicular membrane of mouse hepatocytes, which plays an essential role for bile formation. Mutations in human homologue MDR3 are associated with several liver diseases....
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgv156
更新日期:2016-01-01 00:00:00
abstract::The cyclooxygenase-2 (COX-2) inhibitor celecoxib and the ornithine decarboxylase (ODC) inhibitor difluoromethylornithine (DFMO) were each previously shown to prevent skin tumor development when administered throughout the course of UV irradiation. This raised the question of whether maintenance or continued growth of ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgg046
更新日期:2003-05-01 00:00:00
abstract::Lipid peroxidation aldehydes of the 4-hydroxy-alpha, beta-unsaturated type, as well as the tobacco-smoke related alpha, beta-unsaturated aldehyde, acrolein, were highly cytotoxic and decreased the intracellular thiol content in cultured human bronchial fibroblasts after treatment with micromolar concentrations. In com...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.12.1755
更新日期:1985-12-01 00:00:00
abstract::We previously demonstrated that fasting/refeeding enhances the initiation phase of liver and colorectal carcinogenesis in rats. The present study was undertaken to establish whether cycles of fasting/refeeding carried out during the promotion phase of carcinogenesis may also affect the formation of aberrant crypt foci...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.2.323
更新日期:2002-02-01 00:00:00
abstract::The effects of dietary Brussels sprouts and indole-3-carbinol (I3C) on xenobiotic-metabolizing enzyme activities and hepatic aflatoxin B1 (AFB1)-DNA binding were determined in rats. Animals were dosed intraperitoneally (i.p.) or intragastrically (i.g.) with [3H]AFB1 and killed 2 (i.p.) or 3 (i.g.) h later. Brussels sp...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.4.629
更新日期:1989-04-01 00:00:00
abstract::It is widely believed that abnormal production of polypeptide growth factors, together with other molecular alterations, play an important role in neoplastic development. Transforming growth factor alpha (TGFalpha), hepatocyte growth factor (HGF) and acidic fibroblast growth factor (aFGF) are the three major growth fa...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.5.931
更新日期:1996-05-01 00:00:00
abstract::Treatment of murine Swiss 3T3 fibroblasts and XB/2 keratinocytes with UV-B light (302 nm) resulted in a dose-dependent inhibition of [125I]epidermal growth factor (EGF) binding. The light dose required to achieve 50% inhibition of binding in both cell types was 80-85 J/m2. Decreased [125I]platelet-derived growth facto...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.7.1223
更新日期:1990-07-01 00:00:00
abstract::The landscape of HPV infection in racial/ethnic subgroups of head and neck cancer (HNC) patients has not been evaluated carefully. In this study, a meta-analysis examined the prevalence of HPV in HNC patients of African ancestry. Additionally, a pooled analysis of subject-level data was also performed to investigate H...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgw203
更新日期:2017-02-01 00:00:00
abstract::The pulmonary adenoma susceptibility 1 (Pas1) gene affects susceptibility to the development of lung adenomas in mice with a subset of the adenomas progressing to adenocarcinoma (ADC). In this study, genotype distributions for 10 polymorphisms in the human counterparts for three mouse candidate Pas1 genes, KRAS, CASC1...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn048
更新日期:2008-05-01 00:00:00
abstract::We have measured the levels of thymine glycol (TG) and thymidine glycol (dTG) in human urine, using an HPLC method. The results show that all 30 specimens examined (including 10 non-neoplastic and 20 neoplastic human urines) contained significant amounts of TG and dTG, average levels (mean +/- SEM) were 0.376 +/- 0.02...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.7.1359
更新日期:1993-07-01 00:00:00
abstract::An accurate biomarker or method for diagnosis of thyroid nodule with indeterminate fine-needle aspiration result is essential for clinical treatment. Micro RNAs (miRNAs) of exosomes are advantageous in the diagnosis of tumors because they are highly stable, and be protected by a bilayer membrane structure. Exosomes we...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgz160
更新日期:2020-03-13 00:00:00
abstract::Transgenic mice containing one copy of hepatitis B virus (HBV) genome without the core gene and expressing hepatitis B surface antigen (HBsAg) were crossed with C3H/He mice. The F1 hybrids (approximately 50% HBV positive and approximately 50% HBV negative) were treated with a single dose of diethylnitrosamine (NDEA) o...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.6.953
更新日期:1990-06-01 00:00:00
abstract::Colorectal cancer (CRC) is the fourth leading cause of cancer mortality worldwide. Genome-wide association studies (GWAS) identified more than 50 CRC loci. However, most of the previous studies were conducted in European population, and host genetic factors among Japanese population are largely remained to be identifi...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgy026
更新日期:2018-05-03 00:00:00
abstract:UNLABELLED:Neurotrophic factors possess an emerging role in the pathophysiology of several gastrointestinal disorders, regulating innervation, pain sensation and disease-associated neuroplasticity. Here, we aimed at characterizing the role of the neurotrophic factor neurturin (NRTN) and its receptor glial-cell-line-der...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt312
更新日期:2014-01-01 00:00:00
abstract::To indicate how readily nitrosamines (NAms) diffuse into the esophagus, we measured diffusion rate (flux) through rat esophagus of dialkyl-NAms using side-by-side diffusion apparatuses. Mucosal and serosal flux at 37 degrees C of two NAms, each at 50 microM, was followed for 90 min by gas chromatography-thermal energy...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.5.825
更新日期:1999-05-01 00:00:00
abstract::This study analyzes the mechanism by which the tumor promoter 12-0-tetradecanoyl-phorbol 13-acetate (TPA) inhibits binding of epidermal growth factor (EGF) to its membrane receptors in HeLa cells. Kinetic studies indicate that inhibition of EGF binding occurs within a few minutes after exposure of cells to TPA; delaye...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/1.8.669
更新日期:1980-08-01 00:00:00