Abstract：
INTRODUCTION:Nonequilibrium plasma has been reported to effectively kill Enterococcus faecalis in endodontic biofilm compared with chlorhexidine digluconate (CHX). The purpose of this study was to evaluate the antimicrobial in vitro activity of modified nonequilibrium plasma with CHX against E. faecalis and multispecies biofilms on bovine dentin discs. METHODS:Sterile bovine dentin discs were incubated with E. faecalis or a mixture of bacteria from human dental root canal infections to form 1- and 3-week-old biofilms. The specimens were subjected to nonequilibrium plasma, modified nonequilibrium plasma with CHX, and 2% CHX for 2- and 5-minute exposure. After treatment, the biofilms were stained with viability dyes and examined by confocal laser scanning microscopy and 3-dimensional reconstruction analysis. The proportions of bacterial cells killed by the treatments were calculated. RESULTS:The 3-dimensional reconstruction images showed that 1- and 3-week-old biofilms adhered to bovine dentin discs. The proportions of dead cells increased significantly with the longer exposure in each treatment group (P < .05). Modified nonequilibrium plasma was the most effective in killing bacteria in E. faecalis and multispecies biofilms at both 2 and 5 minutes (P < .05). No significant difference was detected between nonequilibrium plasma and CHX groups (P > .05). Significantly more cells were killed in 1-week-old biofilms than in 3-week-old biofilms in all groups (P < .05). CONCLUSIONS:The modified nonequilibrium plasma killed more bacteria than conventional nonequilibrium plasma and 2% CHX in E. faecalis and multispecies endodontic biofilms in vitro and thus shows promise as an additional tool in infection control during endodontic treatment.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Du T,Shi Q,Shen Y,Cao Y,Ma J,Lu X,Xiong Z,Haapasalo Mdoi
10.1016/j.joen.2013.06.027subject
Has Abstractpub_date
2013-11-01 00:00:00pages
1438-43issue
11eissn
0099-2399issn
1878-3554pii
S0099-2399(13)00590-6journal_volume
39pub_type
杂志文章abstract：:The shape of root canals cross-sectioned through their roots at 2 mm from their apices and its correlation with the D0 diameter of endodontic instruments was evaluated in 40 first upper molars. The molars were grouped according to age: under 13 yr (children), 18 to 20 yr (adolescents), 30 to 40 yr (adults), and over 5...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80357-4
更新日期：1999-10-01 00:00:00
abstract：:Primary intraosseous salivary gland tumors are rare, with mucopidermoid carcinoma being the most frequent histotype. The authors present a case of adenoid cystic carcinoma, located in the mandibular incisor region, associated with pain. Endodontic treatment resulted in increased pain and progressive mandibular expansi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200012000-00026
更新日期：2000-12-01 00:00:00
abstract：INTRODUCTION:Fractured endodontic files present a major problem. A novel method has been proposed to retrieve fractured nickel-titanium (NiTi) endodontic files by using electrochemical dissolution. However, the effect of file dissolution on adjacent soft tissues such as the periodontal ligament (PDL) has not been inves...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.12.013
更新日期：2013-05-01 00:00:00
abstract：:Taurodontism is a morphoanatomical change in the shape of a tooth, which usually occurs in multirooted teeth. An enlarged body and pulp chamber, as well as apical displacement of the pulpal floor, are characteristic features. Endodontic treatment of a taurodont tooth is challenging, because it requires special care in...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200305000-00009
更新日期：2003-05-01 00:00:00
abstract：:The purpose of this survey was to investigate the frequency and characteristics of surgery among active endodontists in the United States. The survey also inquired about residency and postresidency training in endodontic surgery. A Web-based questionnaire regarding endodontic surgery was distributed via e-mail to 2,53...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.10.008
更新日期：2009-01-01 00:00:00
abstract：:K-type files, Sureflex NiTi files, and FlexoFiles were evaluated morphometrically as they come from the manufacturer, and after being used to instrument root canals 1, 3, and 5 times in maxillary premolars. Evaluation was made using a stereomicroscope at x40 magnification and photomicrographs. The abnormalities found ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(98)80046-0
更新日期：1998-07-01 00:00:00
abstract：:The aim of this study was to determine the effects of patient's age on the prevalence and depth of bacterial penetration inside dentinal tubules. Fifty-six single-rooted teeth were divided in two groups based on the patient's age: young (ages 18-25) and old (age >or=60). Teeth were instrumented and inoculated with a s...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.10.004
更新日期：2009-01-01 00:00:00
abstract：INTRODUCTION:Alkaline phosphatase (ALP) plays an important role in inducing mineralization events in the dental pulp. This study investigated and compared the ALP levels in healthy and inflamed pulp in young and old human pulp. METHODS:Tissue samples were collected from young (<30 years) and old (>60 years) donors. In...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.10.003
更新日期：2016-01-01 00:00:00
abstract：INTRODUCTION:The present study aimed to evaluate the antibacterial effect of a new peptide, DJK-5, used alone or mixed together with EDTA on mono- and multispecies biofilms in dentin canals covered by a smear layer with or without preceding sodium hypochlorite (NaOCl) irrigation. METHODS:One hundred twelve dentin bloc...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.07.018
更新日期：2018-11-01 00:00:00
abstract：INTRODUCTION:The primary aim was to explore the criteria used in characterization of reparative cells and mineralized matrices formed after treatment of pulp exposures, and the sequence of relative events. The secondary aim was to evaluate whether the reparative events depend on the experimental model species, age, and...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.12.002
更新日期：2019-03-01 00:00:00
abstract：:This study evaluated the degree of conversion of one dual-cured resin cement when used to lute fiber posts with different translucencies. To measure the degree of conversion, polyvinylsiloxane molds were prepared to simulate root canals. The posts, Aestheti-Post or Light-Post, were cemented in these molds and, after p...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.11.015
更新日期：2007-03-01 00:00:00
abstract：:The purpose of this study was to determine the incidence of endodontic services provided to participants in a longitudinal cohort study. The "Florida Dental Care Study" was a prospective cohort study using a representative baseline sample of 873 dentate adults. An in-person interview and clinical dental exam were cond...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200312000-00005
更新日期：2003-12-01 00:00:00
abstract：:The purposes of this study were to determine quantitatively the amount of debris and irrigant forced in an apical direction, the frequency of apical plug development, and the time required to prepare canals when a step-back technique using K-files was compared with the .04 Taper system. Sixty-nine extracted teeth with...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80206-9
更新日期：1998-01-01 00:00:00
abstract：:Historically, pulp-necrotizing agents were commonly used in endodontic treatments. They act quickly and devitalize the pulp within a few days. However, they are cytotoxic to gingiva and bone. If such an agent diffuses out of the cavity, it can readily cause widespread necrosis of gingiva and bone, which can lead to os...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000125881.18872.ef
更新日期：2004-10-01 00:00:00
abstract：:This study compared the microshear bond strength of three resin-based sealers to root dentin and assessed whether sealer cements behave differently in thin and thick films. Extracted maxillary premolars were sectioned buccolingually, and 45 root halves were randomly allocated for microshear bond testing with the three...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.10.009
更新日期：2009-01-01 00:00:00
abstract：:The cross-sectional configuration of seven files at coronal and apical levels were compared with diagrams presented by manufacturers. The presence of cutting edges was also examined. Ground-twisted (K-file, K-Reamer, and K-flex) and machined files (Flex-R, Unifile, S-file, and Hedstrom) were included in #10, #25, and ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81111-8
更新日期：1995-12-01 00:00:00
abstract：INTRODUCTION:Candida in endodontic infections has been investigated in a large number of studies, but its role as an endodontic pathogen is still debatable. The aim of this study was to systematically review the literature on the prevalence of Candida species in root canal infections. METHODS:Extensive literature rese...
journal_title：Journal of endodontics
pub_type： 杂志文章,meta分析
doi：10.1016/j.joen.2018.07.016
更新日期：2018-11-01 00:00:00
abstract：:The external cervical root resorption associated with intracoronal bleaching of pulpless teeth can be a devastating lesion. It often cannot be repaired. To prevent this problem, increasing attention has been focused on placing a barrier between the pulp chamber and the endodontic filling material. The objective of thi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80822-8
更新日期：1994-06-01 00:00:00
abstract：:This in vitro study compared the accuracy of file length measurements made on calibrated and uncalibrated direct digital images. Endodontic files of known lengths and ISO sizes were used in 10 single-rooted, relatively straight teeth within cadaver specimens. The crowns of the teeth were ground flat and an orthodontic...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200112000-00017
更新日期：2001-12-01 00:00:00
abstract：:Ninety extracted human teeth with a single canal and a large patent foramen were obturated by one of three methods after placement of a master gutta-percha cone and sealer. The apical seals were evaluated by ink penetration. Statistical analysis of the results indicated that the group obturated with the high-temperatu...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(89)80052-4
更新日期：1989-07-01 00:00:00
abstract：INTRODUCTION:Root resorption is a common complication after replantation following traumatic dental avulsion. Endodontic therapy combined with local and intracanal medications aims to avoid osteoclastic activity. In such cases, the application of alendronate (ALN), a bisphosphonate widely used for the treatment of bone...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.07.010
更新日期：2015-01-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to examine the effect of mineral trioxide aggregate (MTA) on pluripotent-mesenchymal cell differentiation. METHODS:The pluripotent-mesenchymal cell line C2C12 was cultured in a 5% serum medium to induce cell differentiation with or without MTA. The differentiation to myoblast...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.10.006
更新日期：2013-01-01 00:00:00
abstract：:This study compared the effectiveness of four different techniques used for obturation of single rooted-teeth: lateral condensation, low-temperature gutta-percha (Ultrafil), vertical condensation of gutta-percha softened by means of three different laser devices (argon, CO2, and Nd:YAG), or composite resin photopolyme...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81530-X
更新日期：1995-09-01 00:00:00
abstract：INTRODUCTION:The aim of this in vitro study was to compare the effectiveness of saline, 2.5% sodium hypochlorite, and 2% chlorhexidine, with or without passive ultrasonic irrigation (PUI), in debris removal from simulated canal irregularities within prepared root canals. METHODS:Ninety bovine lateral incisors were ran...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.08.006
更新日期：2014-12-01 00:00:00
abstract：INTRODUCTION:Phentolamine mesylate has been reported to be an effective local anesthetic reversal agent for soft tissue but has not been studied regarding reversal of pulpal anesthesia. The authors conducted a prospective randomized, single-blind study comparing the reversal of pulpal and soft tissue anesthesia when ph...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2012.12.024
更新日期：2013-04-01 00:00:00
abstract：:The aim of this study was to investigate the cytotoxic and nitric oxide (NO)-inducing effects of bismuth oxide (Bi(2)O(3))-containing Portland cement (BPC) on human dental pulp cells. We also assessed whether heme oxygenase-1 (HO-1) is involved in BPC-induced cytotoxicity in dental pulp cells. Cytotoxicity and NO prod...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.07.012
更新日期：2007-11-01 00:00:00
abstract：INTRODUCTION:Many studies have investigated the morphology of the mandibular molar, but the prevalence of the middle mesial (MM) canal in the mesial root of the mandibular molar is still the subject of controversy. In addition, in previous literature, a true MM canal has not been clearly distinguished from an isthmus b...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.02.008
更新日期：2017-07-01 00:00:00
abstract：INTRODUCTION:The aim was to present a novel surgical technique using virtually preplanned 3-dimensional (3D)-printed templates for guided osteotomies. These were to ensure atraumatic uncovering of a severely impacted donor tooth including guided drilling of the recipient alveolus followed by a secure autotransplantatio...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.07.024
更新日期：2020-07-29 00:00:00
abstract：:The authors reviewed the literature evaluating the antibacterial effectiveness of intracanal medicaments used in the management of apical periodontitis. A PICO (problem, intervention, comparison, outcome) strategy was developed to identify studies dealing with calcium hydroxide, phenolic derivatives, iodine-potassium ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000129959.20011.ee
更新日期：2004-10-01 00:00:00
abstract：:Interactions between Enterococcus faecalis and other species found in root canal infections might be important for the development and persistence of periapical disease. The aim of this study was to investigate the coaggregation interactions between E. faecalis clinical isolates and species previously shown to survive...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.03.023
更新日期：2006-10-01 00:00:00
