Abstract:
OBJECTIVES:Published transmission electron microscopy analysis of in vitro resin-dentin bonds shows that, after 44 months, almost 70% of collagen fibrils from the hybrid layer disappear. Matrix metalloproteinases (MMPs) play an important role in that process and are thought to be the main factor responsible for the solubilization of dentin collagen. Therefore, this study aimed to evaluate the inactivation of matrix-bound MMPs by two different cross-linking agents, carbodiimide (EDC) or proanthocyanidin (PA), or the MMP-inhibitor, chlorhexidine (CHX), on acid-etched dentin using a simplified MMP assay method. MATERIALS AND METHODS:Dentin beams (2×1×6 mm) were obtained from mid-coronal dentin of sound third molars and randomly divided into six groups (G) according to the dentin treatment: G1: Deionized water (control); G2: 0.1 M EDC; G3: 0.5 M EDC; G4: 0.5 M EDC + 35% hydroxyethyl methacrylate (HEMA); G5: 5% PA; and G6: 2% CHX. The beams were etched for 15 seconds with 37% phosphoric acid, rinsed, and then immersed for 60 seconds in one of the treatment solutions. The data were expressed both in absorbance values at 412 nm and in MMP-9 activity equivalents. The total MMP activity of dentin was analyzed for one hour by colorimetric assay (Sensolyte). Data were submitted to Wilcoxon nonparametric test and Mann-Whitney tests (p>0.05). RESULTS:All experimental cross-linking solutions significantly reduced MMP activity from 79.8% to 95.2% when compared to the control group. No difference was observed among 0.1 M EDC (84.8%), 5% PA (87.6%), and 2% CHX (79.8%). Addition of 35% HEMA to 0.5 M EDC produced inactivation (95.2%) that was similar to that of 0.5 M EDC alone (92.7%). CONCLUSION:Dentin treatment with cross-linking agents is effective to significantly reduce MMP activity. Mixing 0.5 M EDC and 35% HEMA did not influence EDC inhibitor potential.
journal_name
Oper Dentjournal_title
Operative dentistryauthors
Scheffel DL,Hebling J,Scheffel RH,Agee K,Turco G,de Souza Costa CA,Pashley Ddoi
10.2341/12-425-Lsubject
Has Abstractpub_date
2014-03-01 00:00:00pages
152-8issue
2eissn
0361-7734issn
1559-2863journal_volume
39pub_type
杂志文章,随机对照试验abstract::The current study investigated the influence of carbamide peroxide on the flexural strength of tooth-colored restorative materials at two environmental temperatures. Seven restorative materials were used, including four resin composites (Filtek Z350, Filtek Z250, Synergy Flow and Filtek P60), a polyacid-modified compo...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/09-139-L
更新日期:2010-05-01 00:00:00
abstract:OBJECTIVES::The use of high-viscosity glass-ionomer cements (HVGICs) for atraumatic restorative treatment (ART) restorations is widely practiced with the advent of various HVGICs. However, the bonding of the latter to caries-affected dentin (CAD) should be validated, especially because it is the common substrate left a...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/17-154-L
更新日期:2018-11-01 00:00:00
abstract::This study compared the post-gel contraction of two "low-shrinkage" composites (InTen-S [IS], Ivoclar-Vivadent; Aelite LS [AL], BISCO Inc) and an ormocer (Admira [AM], Voco) to two conventional mini-filled composites (Renew [RN], BISCO; Z100 [ZO], 3M ESPE). A strain-monitoring device and test configuration were used t...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:
更新日期:2004-03-01 00:00:00
abstract:CLINICAL RELEVANCE:Using a material that optimizes marginal seal when using a margin elevation technique to manage deep class II cavities should enhance clinical outcomes. SUMMARY:Objectives: The purpose of this laboratory study was to perform a tridimensional interfacial gap evaluation of class II cavities with ename...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/19-161-L
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVES:To evaluate and compare the fracture toughness of dental CAD/CAM materials of different material classes intended for in-office milling (glass ceramics, hybrid, resin composites) and the influence of aging on this property. METHODS AND MATERIALS:The fracture toughness (critical intensity factor, KIc) values...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/18-161-L
更新日期:2019-07-01 00:00:00
abstract::This study determined the relationship between marginal adaptation to dentin cavity preparations, tensile bond strength of the restorations, and mechanical properties of the composites. Contraction gaps, tensile bond strengths, flexural strengths, and Young's modulus of eight commercial resin composites were determine...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract:PURPOSE:This prospective long-term clinical trial evaluated and compared the three-year clinical performance of an ormocer, a nanofilled, and a nanoceramic resin composite with that of a microhybrid composite placed in Class I and Class II cavities. METHODS:Forty patients, each with four Class I and II restorations un...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/12-313-C
更新日期:2014-01-01 00:00:00
abstract::A technique of restoring Class II posterior restorations with resin-based composite and a pre-polymerized composite insert was presented. This technique is intended to aid the practitioner in obtaining tight, broad, proximal contacts without having to purchase special instruments or materials. This method is not time-...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:
更新日期:2004-07-01 00:00:00
abstract::This in-vitro study compared the resistance of extensive amalgam restorations retained by either four Regular TMS Link Plus pins, four amalgapins, Amalgambond Plus, Amalgambond Plus with HPA, Scotchbond Multi-Purpose Plus, PQ Amalgam, Panavia F 2.0, All-Bond 2 or Resinomer. Ninety caries-free third molars were embedde...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/08-5
更新日期:2008-11-01 00:00:00
abstract::The objective of this paper is to review the literature concerning factors affecting adhesion to mineralized tissues. Factors related to the physicochemical structure of the adherents and to the inherent properties of composite restorative materials, along with the postulated bonding mechanisms of current adhesive sys...
journal_title:Operative dentistry
pub_type: 杂志文章,评审
doi:
更新日期:1992-01-01 00:00:00
abstract::Marginal adaptation to cylindrical dentin preparations of five commercially available dental composites--Charisma, Estelite, Herculite, Pertac, and Silux Plus--and seven experimental dental composites containing prepolymerized filler was evaluated by measurement of the wall-to-wall polymerization contraction gaps. The...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::The effect of microabrasion on human enamel has been well documented; however, no information is available on its effect on dentin or restorative materials. The purpose of this study was to evaluate the effect of the microabrasion technique on the surface roughness of restorative materials and enamel and dentin surfac...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract:OBJECTIVES:To evaluate the effect of minocycline and chlorhexidine pretreatment of acid-etched dentin on the longevity of resin-dentin bond strength (μTBS) and nanoleakage of two-step etch-and-rinse adhesives. METHODS:Before application of Prime & Bond NT and Adper Single Bond 2 in occlusal dentin, the dentin surfaces...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/15-023-L
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVES:This study evaluated the bond strength of luting systems for bonding glass fiber posts to root canal dentin. The hypothesis tested was that there are no differences in bond strength of glass fiber posts luted with different cement systems. METHODS:Forty bovine incisors were randomly assigned to five differe...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/11-035-L
更新日期:2012-01-01 00:00:00
abstract::This study evaluated the effect of cyclic loading on the bond strength of Class II restorations using different composite materials. Class II preparations with gingival margins located in dentin were performed on the mesial surface of 80 bovine incisors. The teeth were randomly allocated to eight groups (n=10) accordi...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/07-65
更新日期:2008-03-01 00:00:00
abstract::This article reports the case of a patient with bilateral hypodontia of the maxillary lateral incisors who was dissatisfied with the outcome of initial orthodontic treatment, highlighting the importance of a multidisciplinary interaction among Restorative Dentistry, Orthodontics, and Implantology to achieve satisfacto...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/11-167-T
更新日期:2012-09-01 00:00:00
abstract::This study evaluated in vitro the necessity of retention grooves in approximal boxes in class 2 preparations with faciolingually wide occlusal extensions (occlusal isthmus width 1.8 mm). Thirty-six class 2 mesio-occlusal cavities were prepared in sound human maxillary premolar teeth and divided into three groups of 12...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::Retained primary teeth in the smile zone can cause patient dissatisfaction, especially if associated with malposition of teeth. Orthodontic and prosthetic treatment options to treat the situation may not be accepted by some patients. Therefore, these patients tend to maintain their primary teeth and seek different est...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/15-363-S
更新日期:2017-03-01 00:00:00
abstract:INTRODUCTION:Limited access to interdental spaces complicates removal of excess material when placing class II composite resin restorations. Evidence-based recommendations on interproximal finishing are rare. We present novel microscalpels for this indication. The aim of the study was to test their fracture strength an...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/16-012-L
更新日期:2017-05-01 00:00:00
abstract::This study determined the degree of conversion of two LED (light-emitting diodes) (Elipar FreeLight [FL], 3M ESPE; GC e-Light [EL], GC), a high intensity (Elipar TriLight [TL], 3M ESPE) and a very high intensity (Astralis 10 [AS], Ivoclar Vivadent) halogen light. The degree of conversion of these lights was compared t...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
abstract:INTRODUCTION:Adding antibacterial agents to a dentin conditioner used for a glass ionomer cement (GIC) has been shown to be antibacterial; however, it is not known whether this antimicrobial conditioning agent affects the bond strength to dentin in situ. This study applied GIC to antibacterial conditioned dentin withou...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
abstract::For many years, operative dentistry has been using regenerative approaches to treat dental disease. The use of calcium hydroxide to stimulate reparative or reactionary dentin is clearly an example of such a therapeutic strategy. The advent of tissue engineering is allowing dentistry to move forward in the use of regen...
journal_title:Operative dentistry
pub_type: 杂志文章,评审
doi:10.2341/06-000
更新日期:2006-11-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of mechanical loading on the microtensile bond strength (MTBS) of five adhesive systems to dentin. METHODS:Flat dentin surfaces from human molars were divided into five groups and bonded with total-etch self-priming adhesives (Single Bond, Prime&Bond NT and Prime&Bond XP), two-step sel...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/04-161
更新日期:2006-01-01 00:00:00
abstract::Various core materials with different shades affect the final color of high-translucency monolithic zirconia restorations. The blue core shows the greatest color difference in final zirconia restorations followed by metal, A3 dentin-shade resin core, and white core. ...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/19-238-L
更新日期:2020-12-21 00:00:00
abstract::This in vitro study evaluated microleakage in Class II cavities restored with dental composite and varying light-curing units and the temperature of the composite when subjected to a thermocycling test. Ninety cavities were prepared on the proximal surfaces of bovine teeth and randomly divided according to the light-c...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/10-226-LR1
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the influence of chlorhexidine with different application methods on the microtensile bond strength (microTBS) to dentin in Class I cavities. METHODS:Flat dentinal surfaces with Class I cavities (4 mm long, 4 mm wide, 2 mm deep) in 50 molars were bonded with Adper Single Bond 2 etch-and-rinse adh...
journal_title:Operative dentistry
pub_type: 杂志文章,随机对照试验
doi:10.2341/09-360-L
更新日期:2010-11-01 00:00:00
abstract::This study compared microleakage of composite bonded to etched flattened enamel in seven groups of five extracted maxillary molars. The facial enamel of the molars was ground flat and etched for 20 seconds with 37% phosphoric acid gel. In one group, etching gel was not rinsed but dried only. In four groups, gel was ri...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::This study evaluated the biocompatibility of contemporary bulk-fill resin-based composites (RBCs) including PRG (pre-reacted glass ionomer) materials based on the International Organization for Standardization 10993. In addition, the effect of composite thickness on cytotoxicity was also assessed. Two standard composi...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:10.2341/15-059-L
更新日期:2015-11-01 00:00:00
abstract::This study evaluated the effect of ascorbic acid, 500 mg every eight hours, on bleaching-induced tooth sensitivity. A triple-blind, parallel design, and placebo-controlled randomized clinical trial was conducted on 39 adults. The pills (placebo or ascorbic acid) were administered three times per day for 48 hours; the ...
journal_title:Operative dentistry
pub_type: 杂志文章,随机对照试验
doi:10.2341/12-483-C
更新日期:2014-03-01 00:00:00
abstract::This study investigated the effectiveness of cure and post-gel shrinkage of three visible light-cured composite resins (In Ten-S [IT], Ivoclar Vivadent; Z100 [ZO], 3M-ESPE; Tetric Ceram [TC], Ivoclar Vivadent) when polymerized with a very high intensity (1296 +/- 2 mW/cm2) halogen light (Astralis 10, Ivoclar Vivadent)...
journal_title:Operative dentistry
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00