当前位置： SCI文献检索 > NATURE IMMUNOLOGY期刊下所有文献 > A comprehensive analysis of the effects of the deaminase AID on the transcriptome and methylome of activated B cells.

A comprehensive analysis of the effects of the deaminase AID on the transcriptome and methylome of activated B cells.

Abstract：

:Beyond its well-characterized functions in antibody diversification, the cytidine deaminase AID can catalyze off-target DNA damage and has been hypothesized to edit RNA and mediate DNA demethylation. To comprehensively examine the effects of AID on the transcriptome and the pattern of DNA methylation ('methylome'), we analyzed AID-deficient (Aicda(-/-)), wild-type and AID-overexpressing activated B cells by high-throughput RNA sequencing (RNA-Seq) and reduced-representation bisulfite sequencing (RRBS). These analyses confirmed the known role of AID in immunoglobulin isotype switching and also demonstrated few other effects of AID on gene expression. Additionally, we detected no evidence of AID-dependent editing of mRNA or microRNA. Finally, the RRBS data did not support the proposed role for AID in regulating DNA methylation. Thus, despite evidence of its additional activities in other systems, antibody diversification seems to be the sole physiological function of AID in activated B cells.

journal_name

Nat Immunol

journal_title

Nature immunology

authors

Fritz EL,Rosenberg BR,Lay K,Mihailović A,Tuschl T,Papavasiliou FN

doi

10.1038/ni.2616

subject

Has Abstract

pub_date

2013-07-01 00:00:00

pages

749-55

issue

7

eissn

1529-2908

issn

1529-2916

pii

ni.2616

journal_volume

14

pub_type

杂志文章

相关文献

NATURE IMMUNOLOGY文献大全
  • Quality of TCR signaling determined by differential affinities of enhancers for the composite BATF-IRF4 transcription factor complex.

    abstract：:Variable strengths of signaling via the T cell antigen receptor (TCR) can produce divergent outcomes, but the mechanism of this remains obscure. The abundance of the transcription factor IRF4 increases with TCR signal strength, but how this would induce distinct types of responses is unclear. We compared the expressio...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni.3714

    authors： Iwata A,Durai V,Tussiwand R,Briseño CG,Wu X,Grajales-Reyes GE,Egawa T,Murphy TL,Murphy KM

    更新日期：2017-05-01 00:00:00

  • Chemokines: immunology's high impact factors.

    abstract：:Chemokines facilitate leukocyte migration and positioning as well as other processes such as angiogenesis and leukocyte degranulation. The burgeoning knowledge on chemokines and their receptors has influenced many aspects of immunology, in part because cell migration is intimately related to leukocyte function. This o...

    journal_title：Nature immunology

    pub_type： 杂志文章,评审

    doi：10.1038/84298

    authors： Mackay CR

    更新日期：2001-02-01 00:00:00

  • Trans-presentation of IL-6 by dendritic cells is required for the priming of pathogenic TH17 cells.

    abstract：:The cellular sources of interleukin 6 (IL-6) that are relevant for differentiation of the TH17 subset of helper T cells remain unclear. Here we used a novel strategy for the conditional deletion of distinct IL-6-producing cell types to show that dendritic cells (DCs) positive for the signaling regulator Sirpα were ess...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni.3632

    authors： Heink S,Yogev N,Garbers C,Herwerth M,Aly L,Gasperi C,Husterer V,Croxford AL,Möller-Hackbarth K,Bartsch HS,Sotlar K,Krebs S,Regen T,Blum H,Hemmer B,Misgeld T,Wunderlich TF,Hidalgo J,Oukka M,Rose-John S,Schmidt-Supp

    更新日期：2017-01-01 00:00:00

  • Impaired dendritic cell maturation and increased T(H)2 responses in PIR-B(-/-) mice.

    abstract：:Mice deficient for paired immunoglobulin (Ig)-like receptor B (PIR-B) show defective regulation of receptor-mediated activation in antigen-presenting cells. Older PIR-B(-/-) mice had an increased number of peritoneal B1 cells. Splenic PIR-B(-/-) B2 cells were constitutively activated and proliferated much more than th...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni801

    authors： Ujike A,Takeda K,Nakamura A,Ebihara S,Akiyama K,Takai T

    更新日期：2002-06-01 00:00:00

  • Intrahepatic myeloid-cell aggregates enable local proliferation of CD8(+) T cells and successful immunotherapy against chronic viral liver infection.

    abstract：:Chronic infection is difficult to overcome because of exhaustion or depletion of cytotoxic effector CD8(+) T cells (cytotoxic T lymphoytes (CTLs)). Here we report that signaling via Toll-like receptors (TLRs) induced intrahepatic aggregates of myeloid cells that enabled the population expansion of CTLs (iMATEs: 'intra...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni.2573

    authors： Huang LR,Wohlleber D,Reisinger F,Jenne CN,Cheng RL,Abdullah Z,Schildberg FA,Odenthal M,Dienes HP,van Rooijen N,Schmitt E,Garbi N,Croft M,Kurts C,Kubes P,Protzer U,Heikenwalder M,Knolle PA

    更新日期：2013-06-01 00:00:00

  • Autophagy enforces functional integrity of regulatory T cells by coupling environmental cues and metabolic homeostasis.

    abstract：:Regulatory T (Treg) cells respond to immune and inflammatory signals to mediate immunosuppression, but how the functional integrity of Treg cells is maintained under activating environments is unclear. Here we show that autophagy is active in Treg cells and supports their lineage stability and survival fitness. Treg c...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni.3365

    authors： Wei J,Long L,Yang K,Guy C,Shrestha S,Chen Z,Wu C,Vogel P,Neale G,Green DR,Chi H

    更新日期：2016-03-01 00:00:00

  • Monocytes-macrophages that express α-smooth muscle actin preserve primitive hematopoietic cells in the bone marrow.

    abstract：:Hematopoietic stem and progenitor cells (HSPCs) are regulated by various bone marrow stromal cell types. Here we identified rare activated bone marrow monocytes and macrophages with high expression of α-smooth muscle actin (α-SMA) and the cyclooxygenase COX-2 that were adjacent to primitive HSPCs. These myeloid cells ...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni.2408

    authors： Ludin A,Itkin T,Gur-Cohen S,Mildner A,Shezen E,Golan K,Kollet O,Kalinkovich A,Porat Z,D'Uva G,Schajnovitz A,Voronov E,Brenner DA,Apte RN,Jung S,Lapidot T

    更新日期：2012-11-01 00:00:00

  • Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms.

    abstract：:Bacterial lipopolysaccharide triggers human caspase-4 (murine caspase-11) to cleave gasdermin-D and induce pyroptotic cell death. How lipopolysaccharide sequestered in the membranes of cytosol-invading bacteria activates caspases remains unknown. Here we show that in interferon-γ-stimulated cells guanylate-binding pro...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/s41590-020-0697-2

    authors： Wandel MP,Kim BH,Park ES,Boyle KB,Nayak K,Lagrange B,Herod A,Henry T,Zilbauer M,Rohde J,MacMicking JD,Randow F

    更新日期：2020-08-01 00:00:00

  • CSF-1R, DAP12 and beta-catenin: a ménage à trois.

    abstract：:DAP12-coupled receptors influence signals emanating from Toll-like receptors, integrins and receptors for cytokines and growth factors. New findings indicate that DAP12 also facilitates the ability of CSF-1R, the receptor for M-CSF, to induce the stabilization and nuclear translocation of beta-catenin. ...

    journal_title：Nature immunology

    pub_type： 新闻

    doi：10.1038/ni0709-681

    authors： McVicar DW,Trinchieri G

    更新日期：2009-07-01 00:00:00

  • TLR9 signals after translocating from the ER to CpG DNA in the lysosome.

    abstract：:Microbial DNA sequences containing unmethylated CpG dinucleotides activate Toll-like receptor 9 (TLR9). We have found that TLR9 is localized to the endoplasmic reticulum (ER) of dendritic cells (DCs) and macrophages. Because there is no precedent for immune receptor signaling in the ER, we investigated how TLR9 is act...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni1028

    authors： Latz E,Schoenemeyer A,Visintin A,Fitzgerald KA,Monks BG,Knetter CF,Lien E,Nilsen NJ,Espevik T,Golenbock DT

    更新日期：2004-02-01 00:00:00

  • Regulation of lymphocyte development by Notch signaling.

    abstract：:Notch molecules are well conserved from Drosophila melanogaster to mammals and regulate a broad spectrum of various cell lineage commitment processes. Recent studies using inhibitors, transgenic mice and conditional loss-of-function approaches have demonstrated essential roles for Notch signaling in the differentiatio...

    journal_title：Nature immunology

    pub_type： 杂志文章,评审

    doi：10.1038/ni1453

    authors： Tanigaki K,Honjo T

    更新日期：2007-05-01 00:00:00

  • The transcription factor ATF7 mediates lipopolysaccharide-induced epigenetic changes in macrophages involved in innate immunological memory.

    abstract：:Immunological memory is thought to be mediated exclusively by lymphocytes. However, enhanced innate immune responses caused by a previous infection increase protection against reinfection, which suggests the presence of innate immunological memory. Here we identified an important role for the stress-response transcrip...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni.3257

    authors： Yoshida K,Maekawa T,Zhu Y,Renard-Guillet C,Chatton B,Inoue K,Uchiyama T,Ishibashi K,Yamada T,Ohno N,Shirahige K,Okada-Hatakeyama M,Ishii S

    更新日期：2015-10-01 00:00:00

  • Defective survival of naive CD8+ T lymphocytes in the absence of the beta3 regulatory subunit of voltage-gated calcium channels.

    abstract：:The survival of T lymphocytes requires sustained, Ca(2+) influx-dependent gene expression. The molecular mechanism that governs sustained Ca(2+) influx in naive T lymphocytes is unknown. Here we report an essential role for the beta3 regulatory subunit of voltage-gated calcium (Ca(v)) channels in the maintenance of na...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni.1793

    authors： Jha MK,Badou A,Meissner M,McRory JE,Freichel M,Flockerzi V,Flavell RA

    更新日期：2009-12-01 00:00:00

  • Mechanosensing through immunoreceptors.

    abstract：:The immune response is orchestrated by a variety of immune cells. The function of each cell is determined by the collective signals from various immunoreceptors, whose expression and activity depend on the developmental stages of the cell and its environmental context. Recent studies have highlighted the presence of m...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/s41590-019-0491-1

    authors： Zhu C,Chen W,Lou J,Rittase W,Li K

    更新日期：2019-10-01 00:00:00

  • Mycobacterium tuberculosis suppresses innate immunity by coopting the host ubiquitin system.

    abstract：:Mycobacterium tuberculosis PtpA, a secreted tyrosine phosphatase essential for tuberculosis pathogenicity, could be an ideal target for a drug against tuberculosis, but its active-site inhibitors lack selectivity over human phosphatases. Here we found that PtpA suppressed innate immunity dependent on pathways of the k...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni.3096

    authors： Wang J,Li BX,Ge PP,Li J,Wang Q,Gao GF,Qiu XB,Liu CH

    更新日期：2015-03-01 00:00:00

  • ECM1 controls T(H)2 cell egress from lymph nodes through re-expression of S1P(1).

    abstract：:Type 2 helper T cells (T(H)2) are critically involved in allergies and asthma. Here we demonstrate that extracellular matrix protein-1 (ECM1) is highly and selectively expressed in T(H)2 cells. ECM1 deficiency caused impaired T(H)2 responses and reduced allergic airway inflammation in vivo. Functional analysis demonst...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni.1983

    authors： Li Z,Zhang Y,Liu Z,Wu X,Zheng Y,Tao Z,Mao K,Wang J,Lin G,Tian L,Ji Y,Qin M,Sun S,Zhu X,Sun B

    更新日期：2011-02-01 00:00:00

  • The transcription factor musculin promotes the unidirectional development of peripheral Treg cells by suppressing the TH2 transcriptional program.

    abstract：:Although master transcription factors (TFs) are key to the development of specific T cell subsets, whether additional transcriptional regulators are induced by the same stimuli that dominantly repress the development of other, non-specific T cell lineages has not been fully elucidated. Through the use of regulatory T ...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni.3667

    authors： Wu C,Chen Z,Dardalhon V,Xiao S,Thalhamer T,Liao M,Madi A,Franca RF,Han T,Oukka M,Kuchroo V

    更新日期：2017-03-01 00:00:00

  • Production of Ins(1,3,4,5)P4 mediated by the kinase Itpkb inhibits store-operated calcium channels and regulates B cell selection and activation.

    abstract：:Antigen receptor-mediated production of inositol-1,4,5-trisphosphate (Ins(1,4,5)P3) in lymphocytes triggers the release of Ca2+ from intracellular stores; this release of Ca2+ results in the opening of store-operated Ca2+ channels in the plasma membrane. Here we report that mice lacking Ins(1,4,5)P3 3-kinase B (Itpkb)...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni1458

    authors： Miller AT,Sandberg M,Huang YH,Young M,Sutton S,Sauer K,Cooke MP

    更新日期：2007-05-01 00:00:00

  • The human cytomegalovirus microRNA miR-UL112 acts synergistically with a cellular microRNA to escape immune elimination.

    abstract：:Although approximately 200 viral microRNAs are known, only very few share similar targets with their host's microRNAs. A notable example of this is the stress-induced ligand MICB, which is targeted by several distinct viral and cellular microRNAs. Through the investigation of the microRNA-mediated immune-evasion strat...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni.1916

    authors： Nachmani D,Lankry D,Wolf DG,Mandelboim O

    更新日期：2010-09-01 00:00:00

  • Shared and distinct transcriptional programs underlie the hybrid nature of iNKT cells.

    abstract：:Invariant natural killer T cells (iNKT cells) are innate-like T lymphocytes that act as critical regulators of the immune response. To better characterize this population, we profiled gene expression in iNKT cells during ontogeny and in peripheral subsets as part of the Immunological Genome Project. High-resolution co...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni.2490

    authors： Cohen NR,Brennan PJ,Shay T,Watts GF,Brigl M,Kang J,Brenner MB,ImmGen Project Consortium.

    更新日期：2013-01-01 00:00:00

  • Scaffold protein Dlgh1 coordinates alternative p38 kinase activation, directing T cell receptor signals toward NFAT but not NF-kappaB transcription factors.

    abstract：:Tyrosine kinases couple the T cell receptor (TCR) to discrete signaling cascades, each of which is capable of inducing a distinct functional outcome. Precisely how TCR signals are channeled toward specific targets remains unclear. TCR stimulation triggers 'alternative' activation of the mitogen-activated protein kinas...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni1422

    authors： Round JL,Humphries LA,Tomassian T,Mittelstadt P,Zhang M,Miceli MC

    更新日期：2007-02-01 00:00:00

  • Lack of prominent peptide-major histocompatibility complex features limits repertoire diversity in virus-specific CD8+ T cell populations.

    abstract：:Using both 'reverse genetics' and structural analysis, we have examined the in vivo relationship between antigenicity and T cell receptor (TCR) repertoire diversity. Influenza A virus infection of C57BL/6 mice induces profoundly different TCR repertoires specific for the nucleoprotein peptide of amino acids 366-374 (N...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni1175

    authors： Turner SJ,Kedzierska K,Komodromou H,La Gruta NL,Dunstone MA,Webb AI,Webby R,Walden H,Xie W,McCluskey J,Purcell AW,Rossjohn J,Doherty PC

    更新日期：2005-04-01 00:00:00

  • The E3 ligase Itch negatively regulates inflammatory signaling pathways by controlling the function of the ubiquitin-editing enzyme A20.

    abstract：:The ubiquitin-editing enzyme A20 is a critical negative regulator of inflammation and cytokine-mediated activation of the transcription factor NF-kappaB; however, little is known about the mechanisms of A20-mediated inactivation of signaling intermediates such as RIP1. Here we demonstrate that the regulatory molecule ...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni1563

    authors： Shembade N,Harhaj NS,Parvatiyar K,Copeland NG,Jenkins NA,Matesic LE,Harhaj EW

    更新日期：2008-03-01 00:00:00

  • The nonimmunoglobulin portion of lambda5 mediates cell-autonomous pre-B cell receptor signaling.

    abstract：:The pre-B cell receptor (preBCR), composed of mu immunoglobulin (Ig) and surrogate light chains, signals large 'preB-II' cells to proliferate in the apparent absence of ligands or cooperating cells. We deleted the N-terminal, nonimmunoglobulin (nonlg) portion of lambda5, or mutated seven arginine residues in it to ser...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni959

    authors： Ohnishi K,Melchers F

    更新日期：2003-09-01 00:00:00

  • The ER aminopeptidase ERAP1 enhances or limits antigen presentation by trimming epitopes to 8-9 residues.

    abstract：:Endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1) appears to be specialized to produce peptides presented on class I major histocompatibility complex molecules. We found that purified ERAP1 trimmed peptides that were ten residues or longer, but spared eight-residue peptides. In vivo, ERAP1 enhanced production of an ...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni860

    authors： York IA,Chang SC,Saric T,Keys JA,Favreau JM,Goldberg AL,Rock KL

    更新日期：2002-12-01 00:00:00

  • Transcriptomic and epigenetic mechanisms underlying myeloid diversity in the lung.

    abstract：:The lung is inhabited by resident alveolar and interstitial macrophages as well as monocytic cells that survey lung tissues. Each cell type plays distinct functional roles under homeostatic and inflammatory conditions, but mechanisms establishing their molecular identities and functional potential remain poorly unders...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/s41590-019-0582-z

    authors： Sajti E,Link VM,Ouyang Z,Spann NJ,Westin E,Romanoski CE,Fonseca GJ,Prince LS,Glass CK

    更新日期：2020-02-01 00:00:00

  • An essential function for beta-arrestin 2 in the inhibitory signaling of natural killer cells.

    abstract：:The inhibitory signaling of natural killer (NK) cells is crucial in the regulation of innate immune responses. Here we show that the association of KIR2DL1, an inhibitory receptor of NK cells, with beta-arrestin 2 mediated recruitment of the tyrosine phosphatases SHP-1 and SHP-2 to KIR2DL1 and facilitated 'downstream'...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni.1635

    authors： Yu MC,Su LL,Zou L,Liu Y,Wu N,Kong L,Zhuang ZH,Sun L,Liu HP,Hu JH,Li D,Strominger JL,Zang JW,Pei G,Ge BX

    更新日期：2008-08-01 00:00:00

  • Innate immune recognition of viral infection.

    abstract：:Induction of the antiviral innate immune response depends on recognition of viral components by host pattern-recognition receptors. Members of the Toll-like receptor family have emerged as key sensors that recognize viral components such as nucleic acids. Toll-like receptor signaling results in the production of type ...

    journal_title：Nature immunology

    pub_type： 杂志文章,评审

    doi：10.1038/ni1303

    authors： Kawai T,Akira S

    更新日期：2006-02-01 00:00:00

  • An intersection between the self-reactive regulatory and nonregulatory T cell receptor repertoires.

    abstract：:The relationship between the T cell receptor (TCR) repertoires used by self-reactive transcription factor Foxp3-positive (Foxp3(+)) CD4(+) regulatory T cells (T(reg) cells) and nonregulatory T cells with autoimmune potential is unclear. Here we found that the TCR repertoire of thymic T(reg) cells in TCRbeta-transgenic...

    journal_title：Nature immunology

    pub_type： 杂志文章

    doi：10.1038/ni1318

    authors： Hsieh CS,Zheng Y,Liang Y,Fontenot JD,Rudensky AY

    更新日期：2006-04-01 00:00:00

  • Author Correction: c-Kit-positive ILC2s exhibit an ILC3-like signature that may contribute to IL-17-mediated pathologies.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Nature immunology

    pub_type： 已发布勘误

    doi：10.1038/s41590-019-0554-3

    authors： Bernink JH,Ohne Y,Teunissen MBM,Wang J,Wu J,Krabbendam L,Guntermann C,Volckmann R,Koster J,van Tol S,Ramirez I,Shrestha Y,de Rie MA,Spits H,Ros XR,Humbles AA

    更新日期：2020-01-01 00:00:00