Abstract:
:Antimicrobial peptides (AMPs), such as β-defensins and cathelicidins, are essential components of innate and adaptive immunity owing to their extensive multifunctional activities. However, their role in fungal infection in vivo remains elusive. In this study, we investigated the protective effect of murine β-defensin 3 (mBD3), mBD4, and the cathelicidin cathelin-related antimicrobial peptide (CRAMP) in a murine model of Fusarium solani keratitis. C57BL/6 mice showed significant corneal disease 1 and 3 days after infection, which was accompanied by enhanced expression of β-defensins and CRAMP. Disease severity was significantly improved 7 days after infection, at which time AMP expression was returning to baseline. Mice deficient in mBD3 (genetic knockout), mBD4 (short interfering RNA knockdown), or CRAMP (genetic knockout) exhibited enhanced disease severity and progression, increased neutrophil recruitment, and delayed pathogen elimination compared to controls. Taken together, these data suggest a vital role for AMPs in defense against F. solani keratitis, a potentially blinding corneal disease.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Kolar SS,Baidouri H,Hanlon S,McDermott AMdoi
10.1128/IAI.00179-13subject
Has Abstractpub_date
2013-08-01 00:00:00pages
2669-77issue
8eissn
0019-9567issn
1098-5522pii
IAI.00179-13journal_volume
81pub_type
杂志文章abstract::Previous studies have noted that the prevalence of certain gonococcal colony types is influenced by the physiological state of the human host. Consequently eight different gonococcal strains were passed on clear typing medium, and opaque (Op) and transparent (Tr) variants of heavily pilated (P++) bacteria were selecte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.2.359-364.1978
更新日期:1978-11-01 00:00:00
abstract::Toll-like receptors 2 and 4 (TLR2 and TLR4) have been found to transduce signals of peptidoglycan (PGN) and lipopolysaccharide (LPS), respectively, for NF-kappa B activation. However, little is known about the expression and regulation of the TLR2 gene in monocytes/macrophages in response to the two typical bacterial ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.2788-2796.2001
更新日期:2001-05-01 00:00:00
abstract::Immunochemically identical components were isolated from water-soluble phases of five Staphylococcus aureus strains by affinity chromatography on fibrinogen-linked Sepharose 4B. The elution was performed with 1 M MgCl2. The component could be isolated from sonicated preparations of whole cells, cell walls, and extrace...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.700-708.1985
更新日期:1985-09-01 00:00:00
abstract::Resistance to cutaneous syphilitic reinfection in strain 2 and strain 13 guinea pigs developed gradually 3 to 7 months after primary infection and reached maximum levels at 6 to 7 months after the induction of primary cutaneous disease. Associated with this acquired resistance was the occurrence of Arthus reactions an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.1.66-72.1985
更新日期:1985-10-01 00:00:00
abstract::Endotoxin-treated mice exhibit a rapid rise in the level of the serum amyloid A (SAA) protein, but this effect is not observed in endotoxin-resistant C3H/HeJ mice. To evaluate the role of lymphoid cells in the production of SAA protein, C3H/HeJ mice were adoptively transfused with endotoxin-sensitive (C3H/HeN) bone ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.1.181-183.1979
更新日期:1979-01-01 00:00:00
abstract::Mycobacterial growth inhibitory factor (MycoIF), found in supernatant fluids of mouse spleen cell cultures that have been stimulated in vitro with homologous antigen, inhibited the intracellular multiplication of virulent tubercle bacilli within normal mouse peritoneal macrophages in vitro. Antigenically stimulated H...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.4.833-840.1975
更新日期:1975-10-01 00:00:00
abstract::The purified recombinant V antigen from Yersinia pestis, expressed in Escherichia coli and adsorbed to aluminum hydroxide, an adjuvant approved for human use, was used to immunize outbred Hsd:ND4 mice subcutaneously. Immunization protected mice from lethal bubonic and pneumonic plague caused by CO92, a wild-type F1+ s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.11.4580-4585.1996
更新日期:1996-11-01 00:00:00
abstract::Secreted and surface-exposed antigens of intracellular pathogens are thought to provide target structures for detection by the host immune system. The major secreted product of intracellular Leishmania mexicana amastigotes, a proteophosphoglycan (aPPG), is known to contribute to the establishment of the parasitophorou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5379-5385.1999
更新日期:1999-10-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) causes repeated respiratory infections in patients with chronic lung diseases. These infections are characterized by a brisk inflammatory response which results in the accumulation of polymorphonucleated cells in the lungs and is dependent on the expression and secretion of pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.8.4430-4440.2000
更新日期:2000-08-01 00:00:00
abstract::Helicobacter infections are present in approximately 50% of humans, causing severe illnesses such as gastritis and malignancies. Dendritic cells (DC) are critical antigen-presenting cells which link innate and adaptive immune responses. The mechanism of dendritic cell regulation in Helicobacter-induced gastritis is po...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00289-06
更新日期:2006-08-01 00:00:00
abstract::Corynebacterium renale type I (strain 115), 1.7 X 10(7) to 4.5 X 10(7) organisms, introduced intravenously into mice disappeared from the blood less than 24 h after inoculation and did not produce pyelonephritis. The same strain, 1 X 10(7) to 5 X 10(7) organisms, inoculated into the urinary bladder of mice was not rec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.263-267.1977
更新日期:1977-04-01 00:00:00
abstract::The DNA oligomer 5'-d(TGCGGCCTCTCAGTCCCGCACTTTCATCTTCC)-3' specifically recognizes Haemophilus influenzae 16S rRNA. We report here the use of this oligonucleotide, with a fluorescein label tagged on its 5' end, as a probe for the in situ detection of nonencapsulated nontypeable H. influenzae in sections of adenoid tis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.673-679.1994
更新日期:1994-02-01 00:00:00
abstract::Mice (BALB/cJ, C3H/HeN, and C3H/HeJ) primed with actinomycin D became highly susceptible to lethal intoxication with staphylococcal enterotoxin B (SEB). The mice underwent toxicosis and toxic shock and died. Actinomycin D-primed C3H/HeN and C3H/HeJ mice showed equal sensitivity to SEB, suggesting that bacterial lipopo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4626-4631.1994
更新日期:1994-10-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) initiates infection by colonizing the upper respiratory tract and is a common cause of localized respiratory tract disease. Previous work has established that the NTHi HMW1 and HMW2 proteins are potent adhesins that mediate efficient in vitro adherence to cultured human respir...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00153-16
更新日期:2016-09-19 00:00:00
abstract::Bacillus anthracis is surrounded by a polypeptide capsule composed of poly-gamma-d-glutamic acid (gammaDPGA). In a previous study, we reported that a monoclonal antibody (MAb F26G3) reactive with the capsular polypeptide is protective in a murine model of pulmonary anthrax. The present study examined a library of six ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01133-06
更新日期:2007-01-01 00:00:00
abstract::Active immunization of mice with high doses of heat-killed and autoclaved vaccine of unencapsulated strains of Staphylococcus aureus, which was grown in brain heart infusion media, protected against challenge with the Smith diffuse strain of Staphylococcus aureus. These organisms were capable of absorbing the protecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.5.939-942.1975
更新日期:1975-11-01 00:00:00
abstract::Saccharomyces boulardii is a nonpathogenic yeast used in the treatment of Clostridium difficile diarrhea and colitis. We have reported that S. boulardii inhibits C. difficile toxin A enteritis in rats by releasing a 54-kDa protease which digests the toxin A molecule and its brush border membrane (BBM) receptor (I. Cas...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.1.302-307.1999
更新日期:1999-01-01 00:00:00
abstract::Poly-N-acetyl-glucosamine (PNAG) is a staphylococcal surface polysaccharide influencing biofilm formation that is also under investigation for its vaccine potential. Antibodies that bind to PNAG with either low (<15%) or high (>90%) levels of acetate are superior at opsonic and protective activity compared with antibo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00078-07
更新日期:2007-07-01 00:00:00
abstract::The multiple antigen peptide (MAP) approach is an effective method to chemically synthesize and deliver multiple T-cell and B-cell epitopes as the constituents of a single immunogen. Here we report on the design, chemical synthesis, and immunogenicity of three Plasmodium falciparum MAP vaccines that incorporated antig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00533-10
更新日期:2010-11-01 00:00:00
abstract::This study examined apoptotic cell death associated with Shiga-like toxin (Stx)-producing Escherichia coli. Renal cortices from three children with postenteropathic hemolytic-uremic syndrome (HUS) and from mice infected with E. coli O157:H7 and pediatric renal tubular epithelial cells stimulated with Stx and E. coli O...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.636-644.1998
更新日期:1998-02-01 00:00:00
abstract::Plasmodium falciparum apical membrane antigen 1 (AMA1) is a leading malaria vaccine candidate whose function has not been unequivocally defined. Partial complementation of function can be achieved by exchanging the AMA1 of P. falciparum (PfAMA1) with that of P. chabaudi (PcAMA1). In this study, parasites expressing ch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2444-2451.2005
更新日期:2005-04-01 00:00:00
abstract::Borrelia burgdorferi, the bacterium that causes Lyme disease, has a unique segmented genome consisting of numerous linear and circular plasmids and a linear chromosome. Many of these genetic elements have been found to encode factors critical for B. burgdorferi to complete the infectious cycle. However, several plasmi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00219-13
更新日期:2013-08-01 00:00:00
abstract::Endogenous interleukin-12 (IL-12) mediates protection against Yersinia enterocolitica in C57BL/6 mice by triggering gamma interferon (IFN-gamma) production in NK and CD4+ T cells. Administration of exogenous IL-12 confers protection against yersiniae in Yersinia-susceptible BALB/c mice but exacerbates yersiniosis in r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.2213-2220.1998
更新日期:1998-05-01 00:00:00
abstract::One- to three-day-old guinea pigs were inoculated intranasally with the chlamydial agent of guinea pig inclusion conjunctivitis. Physical signs of infection included a marked increase in respiration rate on days 5 to 10 of infection and radiographic evidence of pneumonia on day 6. When animals were killed at various t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.1.153-158.1985
更新日期:1985-04-01 00:00:00
abstract::Relapsing fever (RF) is a spirochetal infection characterized by periods of sickness with fever at time of high bacteremia that alternate with afebrile periods of relative well being during low bacteremia. Patients with epidemic RF who are doing relatively well have extraordinarily high levels of interleukin-10 (IL-10...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00587-08
更新日期:2008-12-01 00:00:00
abstract::Science has always been a competitive undertaking. Despite recognition of the benefits of cooperation and team science, reduced availability of funding and jobs has made science more competitive than ever. Here we consider the benefits of competition in providing incentives to scientists and the adverse effects of com...
journal_title:Infection and immunity
pub_type: 社论
doi:10.1128/IAI.02939-14
更新日期:2015-04-01 00:00:00
abstract::A purified A-antigen preparation of Blastomyces dermatitidis was determined to be composed of five major glycoprotein bands, visible with Coomassie blue and periodic acid-Schiff staining of polyacrylamide gels. At least 20 additional protein bands were detected by using a silver stain, which was 100 times more sensiti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.1.171-177.1981
更新日期:1981-07-01 00:00:00
abstract::EspG is a conserved protein encoded by the locus of enterocyte effacement (LEE) of attaching and effacing (A/E) pathogens, including enteropathogenic and enterohemorrhagic Escherichia coli and Citrobacter rodentium. EspG is delivered into infected host cells by a type III secretion system. The role of EspG in virulenc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.2586-2594.2005
更新日期:2005-05-01 00:00:00
abstract::The Th1/Th2 cytokines involved in human periodontitis remain unclear; therefore, we established a humanized mouse model to investigate this issue in Actinobacillus actinomycetemcomitans-mediated periodontal infection. Quantitative-PCR analysis clearly demonstrates a predominantly mixed Th1 and Th2 expression profile a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.9.5269-5273.2002
更新日期:2002-09-01 00:00:00
abstract::Patent filarial infection is associated with the downregulation of parasite-specific immune reactivity. In the present study, the relationship between in vitro parasite antigen-specific humoral and cellular immune responsiveness was investigated in Brugia pahangi-infected jirds and in jirds immunized with soluble anti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3052-3057.1988
更新日期:1988-12-01 00:00:00