Abstract：
:Gene expression analysis has been established as a tool for the characterization of genotoxic mechanisms of chemical mutagens. It has been suggested that expression analysis is capable of distinguishing compounds that cause DNA damage from those that interfere with mitotic spindle function. Formaldehyde (FA) is known to be a DNA-reactive substance which mainly induces chromosomal damage in cultured mammalian cells. However, there has been concern that FA might also induce leukemia-specific aneuploidies, although recent cytogenetic studies excluded a relevant aneugenic potential of FA. We now investigated whether gene expression profiling can be used as a molecular tool to further characterize FA's genotoxic mode of action and to differentiate between clastogenic and aneugenic activity. TK6 cells were exposed to FA for 4 and 24 h, and changes in gene expression were analyzed using a whole-genome human microarray. Results were compared to the expression profiles of two DNA-damaging clastogens (methyl methanesulfonate and ethyl methanesulfonate) and two aneugens (colcemid and vincristine). The genotoxic activity of FA, MMS and EMS under these conditions was confirmed by comet assay experiments. The gene expression profiles indicated that clastogens and aneugens induce discriminable gene expression patterns. Exposure of TK6 cells to FA led to a discrete gene expression pattern, and all toxicogenomics analyses revealed a closer relationship of FA with clastogens than with aneugens.
journal_name
Arch Toxicoljournal_title
Archives of toxicologyauthors
Kuehner S,Holzmann K,Speit Gdoi
10.1007/s00204-013-1060-2subject
Has Abstractpub_date
2013-11-01 00:00:00pages
1999-2012issue
11eissn
0340-5761issn
1432-0738pii
10.1007/s00204-013-1060-2journal_volume
87pub_type
杂志文章abstract：:The metabolism of arsenic is generally accepted to proceed by repetitive reduction and oxidative methylation; the latter is mediated by arsenic methyltransferase (Cyt19). In human urine, the major metabolites of inorganic arsenicals such as arsenite (iAsIII) and arsenate (iAsV) are monomethylarsonic acid (MMA(V)) and ...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-004-0620-x
更新日期：2005-04-01 00:00:00
abstract：:Using male ICR mice, the LC50 and acute and subacute inhalation toxicity of dichlorosilane (SiH2Cl2, DCS) and the fate of DCS released into the air were investigated. DCS resolved and minute particles including silicon and chloride were observed, when DCS was released into the air. Most particles were under 1 micron i...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s002040050263
更新日期：1996-01-01 00:00:00
abstract：:A predictive test using mice for the identification of contact sensitizing chemicals was developed. Contact sensitizing activity is measured as a function of draining lymph node activation following application of test chemical. Experimental conditions for assessment of induced lymph node cell (LNC) responses have bee...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF01974070
更新日期：1993-01-01 00:00:00
abstract：:The trichothecene mycotoxin deoxynivalenol (DON) is produced in wheat, barley and corn following infestation by the fungus Fusarium in the field and during storage. Colloquially known as "vomitoxin" because of its emetic effects in pigs, DON has been associated with human gastroenteritis. Since DON is commonly detecte...
journal_title：Archives of toxicology
pub_type： 杂志文章,评审
doi：10.1007/s00204-010-0579-8
更新日期：2010-09-01 00:00:00
abstract：:DW-116, 1-(5-fluoro-2-pyridyl)-6-fluoro-7-(4-methyl-l-piperazinyl)-1, 4-dihydro-4-oxoquinolone-3-carboxylic acid hydrochloride, is a newly developed fluoroquinolone antibacterial. The potential of DW-116 to induce developmental toxicity was, investigated in Sprague-Dawley rats. DW-116 was administered by gavage to pre...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s002040050662
更新日期：2000-04-01 00:00:00
abstract：:The current management of rodent pest populations is based on second-generation anticoagulant rodenticides (SGAR). These molecules, of which difethialone is part, are much more efficient than the first generation. Nevertheless, this efficiency comes with a major drawback, SGARs are tissue persistent that increases the...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-020-02662-0
更新日期：2020-03-01 00:00:00
abstract：:pp'DDT (600 mg/kg, orally) produced an increase in the level of free ammonia and a decrease of acetylcholine in the corpus striatum of rats. These effects were maximal 5 h after the administration of pp'DDT. The animals showed tremors after 2 h, mild or moderate convulsions after 3.5 h and severe convulsions 5 h after...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00303292
更新日期：1980-05-01 00:00:00
abstract：:In female rats dosed orally with 14C-hexachlorobenzene the extent of intestinal absorption of carbon-14 has been found to be dependent on the form of application. When the substance was given as a solution in oil about 80% of the dose administered was absorbed, but when given as an aqueous suspension only 6%. In anima...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00353283
更新日期：1975-11-20 00:00:00
abstract：:The organophosphorus compound trichlorfon, dimethyl (2,2,2-trichloro-1-hydroxyethyl) phosphonate, was administered by stomach tube (100 mg/kg) to pregnant guinea pigs at two different stages of gestation (days 36, 37, 38 and 51, 52, 53). The pups developed locomotory disturbances, and post mortem examination revealed ...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00263954
更新日期：1986-05-01 00:00:00
abstract：:The metabolic fate of 2-ethylhexyl diphenyl phosphate (EHDPP) was studied in male rats. Orally administered 14C-EHDPP was rapidly absorbed and about 80% of the radioactivity was excreted in the urine and feces in the first 24 h. By 7 days, 48% and 52% of the radioactivity was recovered in urine and feces, respectively...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00364847
更新日期：1988-01-01 00:00:00
abstract：:The present study was performed to investigate the potential toxic effects of prenatal exposure to 4-tert-octylphenol (OP) on liver, kidney, spleen, and hematologic parameters of male and female rats in adult life. The rats were treated with OP subcutaneously in utero at doses of control (vehicle, corn oil), 100 or 25...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-008-0354-2
更新日期：2009-04-01 00:00:00
abstract：:Certain populations of the mosquitofish (Gambusia affinis) are highly resistant to cyclodiene and cyclodiene-type insecticides that competitively interact with the picrotoxinin binding site of the gamma-aminobutyric acid (GABA) receptor-ionophore complex in the central nervous system. Resistance involves a reduction i...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00332493
更新日期：1988-01-01 00:00:00
abstract：:In order to clarify the possible mechanism of hepatocarcinogenesis induced by piperonyl butoxide, we attempted to identify the transcription factor activated by piperonyl butoxide in the male ICR mouse liver. Administration of 0.6% piperonyl butoxide for 24 h elevated the level of liver nuclear proteins that bind to a...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-008-0283-0
更新日期：2008-10-01 00:00:00
abstract：:The effects of equimolar doses of the triphenylethylene antiestrogens tamoxifen and toremifene on female Sprague-Dawley rat liver were studied in a 52-week toxicity study which included a 13-week recovery period. Liver tumors were found in four out of five rats at the highest dose level of tamoxifen (45 mg/kg per day)...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF02072035
更新日期：1993-01-01 00:00:00
abstract：:The organophosphorus (OP) pesticide malathion is a neurotoxic compound whose acute toxicity is primarily caused by the inhibition of acetylcholinesterase (AChE), leading to cholinergic syndrome-related symptoms. Some lines of evidence indicate that long-term exposure to low levels of OP may produce neuropsychiatric an...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-015-1466-0
更新日期：2016-03-01 00:00:00
abstract：:Pathological changes induced by cadmium aerosol had features common to the changes evoked by oxidants. Female rats were exposed to fumes of lead, antimony, zinc and cadmium (15-100 nmoles/m3). One hour after termination of exposure, superoxide dismutase (SOD) activity in erythrocytes of the exposed rats lowered by 15-...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00347869
更新日期：1982-03-01 00:00:00
abstract：:The in vitro DNA strand breaking activity of metallothionein (MT) containing Cd2+ and Zn2+ in a molar ratio of 5:2 is described. Studies with radical scavengers and electron paramagnetic resonance spectroscopy indicate that the DNA damage might be caused by a radical species formed by the native protein (i.e., MT) cha...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF01973498
更新日期：1991-01-01 00:00:00
abstract：:A recent intervention study demonstrated the occurrence of irilone as second most abundant isoflavone next to daidzein in human plasma after consumption of a red clover-based dietary supplement (RCDS) containing predominately formononetin ≫ biochanin A > irilone (12 % of these isoflavones). To elucidate the relevance ...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-013-1114-5
更新日期：2014-02-01 00:00:00
abstract：:The involvement of oxidative stress has been suggested as a mechanism for neurotoxicity caused by methylmercury (MeHg), but the mechanism for MeHg selective toxicity in the central nervous system is still unclear. In this research, to clarify the mechanism of selective neurotoxicity caused by MeHg, the oxygen consumpt...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-007-0209-2
更新日期：2007-11-01 00:00:00
abstract：:Knowledge about established breast carcinogens can support improved and modernized toxicological testing methods by identifying key mechanistic events. Ionizing radiation (IR) increases the risk of breast cancer, especially for women and for exposure at younger ages, and evidence overall supports a linear dose-respons...
journal_title：Archives of toxicology
pub_type： 杂志文章,评审
doi：10.1007/s00204-020-02752-z
更新日期：2020-05-01 00:00:00
abstract：:Exposure to bisphenolic chemicals during pregnancy occurs in > 90% of pregnancies. Bisphenolic compounds can cross the placental barrier reaching fetal circulation. However, the effects of emerging bisphenolic compounds, such as bisphenol S (BPS), on placental function remain untested. The aim was to determine if bisp...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-018-2191-2
更新日期：2018-05-01 00:00:00
abstract：:Second order rate constants for the initial reaction of 12 mammalian oxyhemoglobins (Hb) with equimolar phenylhydrazine (PHZ), a compound inducing Heinz body hemolytic anemia, were determined by recording continuous changes in absorbance with time at 577 nm. The rate constants were varied in a range from 43 m-1.s-1 wi...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s002040050161
更新日期：1995-01-01 00:00:00
abstract：:Chondrotoxicity is a rare toxicological finding which is observed in dogs after administration of quinolone antibacterials. To study this effect chondrocytes from articular cartilage of dogs were isolated, and incubated with quinolone derivatives. The effects on cell viability, mitochondrial dehydrogenase, and proteog...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF01977402
更新日期：1993-01-01 00:00:00
abstract：:The reduction of 20 microM hexavalent chromium [chromium(VI)] by L-ascorbic acid (AsA) (0.06-2 mM) and/or glutathione (GSH) (2-15 mM) in buffer solutions, cell-free bronchoalveolar lavage fluids or soluble fractions of rat lungs was investigated at physiological pH (37 degrees C). The reduction in AsA solution was pse...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF02010721
更新日期：1990-01-01 00:00:00
abstract：:The effect of acute and chronic cadmium administration on hepatic drug metabolism was investigated in the male rat. 3 days after the acute administration of cadmium by either the intraperitoneal (0.84 mg Cd/kg) or the oral (greater than 80 mg Cd/kg) route, there was a significant potentiation in duration of hexobarbit...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00310332
更新日期：1978-08-09 00:00:00
abstract：:N-methyl-D-aspartate (NMDA) and non-NMDA receptors were found to be involved in development of functional disorders caused by hexachlorophene. In order to specify the role of glutamate receptors we studied the protective effects of the selective antagonist of the kainate/(+/-)-alpha-amino-3-hydroxy-5-methylisoxazole-4...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s002040050011
更新日期：2000-01-01 00:00:00
abstract：:Chronic arsenite exposure induces immunosuppression, but the precise mechanisms remain elusive. Our previous studies demonstrated that arsenite exposure for 24 h induces G0/G1 arrest in mouse B lymphoma A20 cells and the arrest is caused through induction of cyclin-dependent kinase inhibitor p16(INK4a) followed by acc...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-015-1500-2
更新日期：2016-04-01 00:00:00
abstract：:Metabolism of carbaryl by rat liver and skin post-mitochondrial fraction has been measured in the presence and absence of cofactors to promote different metabolic pathways. The metabolic capacity was compared with the metabolism of carbaryl during percutaneous absorption in a static skin diffusion system using a varie...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF01973722
更新日期：1991-01-01 00:00:00
abstract：:Phenobarbital pretreatment (50 mg/kg/day for 3 days orally) of male Wistar rats increased Vmax of benzene in vitro hepatic microsomal biotransformation about 6-fold without changing Km. However, benzene blood levels after oral, intraperitoneal, or subcutaneous benzene administration (3-3.5 mmoles/kg) were not influenc...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00293567
更新日期：1976-06-08 00:00:00
abstract：:Unsaturated compounds are known to cause false-positive reactions in the local lymph node assay (LLNA) but not in the guinea pig maximization test. We have tested a panel of substances (succinic acid, undecylenic acid, 1-octyn-3-ol, fumaric acid, maleic acid, linoleic acid, oleic acid, alpha-linolenic acid, squalene, ...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-015-1527-4
更新日期：2016-04-01 00:00:00
