Abstract:
:Dihydropyrimidinase-related protein 4 (Dpysl4) is a known regulator of hippocampal neuron development. Here, we report that Dpysl4 is involved in growth regulation, polarization and differentiation of dental epithelial cells during tooth germ morphogenesis. A reduction in Dpysl4 gene expression in the tooth germ produced a loss of ameloblasts, resulting in the decrease of synthesis and secretion of enamel. The inhibition of Dpysl4 gene expression led to promotion of cell proliferation of inner enamel epithelial cells and inhibition of the differentiation of these cells into pre-ameloblasts, which was confirmed by analyzing cell polarization, columnar cell structure formation and the expression of ameloblast marker genes. By contrast, overexpression of Dpysl4 in dental epithelial cells induces inhibition of growth and increases the expression of the inner enamel epithelial cell marker gene, Msx2. These findings suggest that Dpysl4 plays essential roles in tooth germ morphogenesis through the regulation of dental epithelial cell proliferation, cell polarization and differentiation.
journal_name
Int J Biol Scijournal_title
International journal of biological sciencesauthors
Yasukawa M,Ishida K,Yuge Y,Hanaoka M,Minami Y,Ogawa M,Sasaki T,Saito M,Tsuji Tdoi
10.7150/ijbs.5510subject
Has Abstractpub_date
2013-04-26 00:00:00pages
382-90issue
4issn
1449-2288pii
ijbsv09p0382journal_volume
9pub_type
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