Abstract:
:One of the main mechanisms for multidrug resistance (MDR) involves multidrug resistance gene 1 (MDR1) which encodes P-glycoprotein (Pgp). Pgp acts as a drug efflux pump and exports chemotherapeutic agents from cancer cells. Specific inhibition of Pgp expression by gene therapy is considered a well-respective strategy having less innate toxicities. At present, the investigation of DRz in reversal MDR is scarce. In the study, phosphorothioate DRz that targets to the translation initiation codon AUG was synthesized and transfected into breast cancer cells and leukemia cells with MDR phenotype. ASODN (antisense oligonucleotide) and ribozyme targets to the same region were also synthesized for comparison analysis. Alterations in MDR1 mRNA and Pgp were determined by RT-PCR, Northern blot, flow cytometry and Rh123 retention tests. Chemosensitivity of the treated cells was determined by MTT assay. The results showed that DRz could significantly suppress expression of MDR1 mRNA and inhibit synthesis of Pgp. The efflux activity of Pgp was inhibited accordingly. Chemosensitivity assay showed that a 21-fold reduction in drug resistance for Adriamycin and a 45-fold reduction in drug resistance for Vinblastine were found in the treated cells 36h after transfection. These data suggest that DRz targeted to the translation initiation codon AUG can reverse MDR phenotype in cancer cells and restore their chemosensitivity. Moreover, the reversal efficiency of DRz is better than that of ribozyme and ASODN targets to the same region of MDR1 mRNA.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Xing AY,Shi DB,Liu W,Chen X,Sun YL,Wang X,Zhang JP,Gao Pdoi
10.1016/j.yexmp.2013.03.004subject
Has Abstractpub_date
2013-06-01 00:00:00pages
481-5issue
3eissn
0014-4800issn
1096-0945pii
S0014-4800(13)00042-7journal_volume
94pub_type
杂志文章abstract:OBJECTIVE:This study was undertaken to investigate the relationship between the Apolipoprotein B/Apolipoprotein A 1 (ApoB/ApoA 1) ratio and various characteristics of the metabolic syndrome (MetS) in a sample of the Tunisian population. METHODS:The study included 330 adults aged 35-74 (172 patients with MetS and 158 c...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2011.06.007
更新日期:2011-10-01 00:00:00
abstract::Transmissible spongiform encephalopathies (TSE) are fatal neurodegenerative diseases of man and animals and are transmitted by a filterable pathogen whose identity is currently unresolved. Our data indicates that Spiroplasma, a wall-less bacterium, is involved in the pathogenesis of TSE. We searched for Spiroplasma ri...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2004.02.002
更新日期:2004-08-01 00:00:00
abstract::It is known that spermidine and other naturally occurring polyamines accumulate in rat lung slices by an energy-dependent uptake process and that alveolar macrophages (AM) have a greater rate of uptake than has the total lung cell population. In the present study rat AMs were incubated with spermidine, which resulted ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1994.1005
更新日期:1994-02-01 00:00:00
abstract::Chemerin is a recently described adipokine whose adipose tissue and serum levels are increased in obesity. Chemerin is expressed in the liver, and here, expression of chemerin has been studied in liver cells and in non-alcoholic fatty liver disease (NAFLD) which is more often found in obesity. Chemerin is shown to be ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2013.07.009
更新日期:2013-10-01 00:00:00
abstract::In the present investigation, we observed that lead in vitro activates calmodulin at lower concentrations, and the maximum activation was observed at 30 microM concentration. In vivo lead exposure (50 mg/kg body weight, intragastrically) for a period of 8 weeks also stimulated the activity of calmodulin by 45%. The ad...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1994.1026
更新日期:1994-08-01 00:00:00
abstract::Retinoblastoma (RB), the most common intra-ocular malignancy in children under 5 years of age, has an estimated incidence of about 2000 a year in India, where most cases are in advanced stage at the time of diagnosis. Newer therapeutic approaches would reduce the morbidity of chemotherapy in children with RB. Fatty Ac...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2010.11.007
更新日期:2011-02-01 00:00:00
abstract::Previously, we described an orthotopic cholangiocarcinoma model based on bile duct inoculation of spontaneously-transformed low grade malignant rat BDE1 cholangiocytes (BDEsp cells) compared to high grade malignant erbB-2/neu- transformed BDE1 cholangiocytes (BDEneu cells) into the livers of syngeneic rats, which clos...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2010.08.007
更新日期:2010-12-01 00:00:00
abstract:INTRODUCTION AND AIMS:Oxytocin (OT) is a neuropeptide hormone secreted by the posterior pituitary gland. Deficits in OT action have been observed in patients with behavioral and mood disorders, some of which correlate with an increased risk of cardiovascular disease (CVD). Recent research has revealed a wider systemic ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2021.104604
更新日期:2021-01-09 00:00:00
abstract::As the fifth most common cancer and the second leading cause of cancer related deaths worldwide, hepatocellular carcinoma (HCC) causes up to one million deaths annually. Alcoholic steatohepatitis (ASH) and non-alcoholic steatohepatitis (NASH) are becoming the two major risk factors because both may develop liver fibro...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2019.04.002
更新日期:2019-06-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2004.01.003
更新日期:2004-06-01 00:00:00
abstract::Partial-thickness skin burns have been shown to induce neutrophil-dependent microvascular injury both locally (skin) and systemically (lung). In the present study, interventional measures to block inflammatory chemoattractants were employed to define the pathophysiologic role of these mediators in the development of s...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1999.2263
更新日期:1999-08-01 00:00:00
abstract::The thrombocyte is the avian equivalent of the mammalian blood platelet and is involved in hemostasis through a fibrinogen-mediated process. Although fibrinogen has been implicated as a molecular bridge between activated cells during aggregation, the location of this molecule and its receptor on thrombocytes has not b...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1994.1035
更新日期:1994-12-01 00:00:00
abstract::Due to the ubiquitous nature of airborne endotoxin, an understanding of pulmonary alterations which follow inhalation of environmentally realistic concentrations of purified bacteria derived lipopolysaccharide (LPS) is important. Using LPS derived from Enterobacter agglomerans, a bacterium found in cotton and cotton m...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(85)90068-1
更新日期:1985-12-01 00:00:00
abstract::Chronic heart failure (CHF) is a common disease in clinical practice, and its incidence has been increasing in recent years. Understanding the pathogenesis of CHF is the key to its future clinical diagnosis and treatment. Molecular research is a hot topic in modern hospitals, and long non-coding RNA (LncRNA) has been ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2020.104529
更新日期:2020-12-01 00:00:00
abstract::Modifications induced by estrogens on hormone-independent murine mammary tumor (MMT) and its main etiological agent, the MMT virus (MMTV), are reported. High doses of estrogens released continuously from silastic capsules delay significantly the development of transplanted tumors into syngeneic hosts. Neoplastic cells...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(89)90053-1
更新日期:1989-02-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2016.04.003
更新日期:2016-08-01 00:00:00
abstract::The development of cyclophosphamide-induced pulmonary lesions over a 1-year period was studied in mice. Male BALB/c mice received a single intraperitoneal injection of 100 mg/kg of cyclophosphamide. Within 3 weeks there were scattered foci of intraalveolar foamy macrophages. With time, these foci increased in size and...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(85)90031-0
更新日期:1985-04-01 00:00:00
abstract::Apolipoprotein E (apoE) is an important plasma protein in cholesterol homeostasis and plays a key role in the progression of glomerulosclerosis (GS). We conducted this investigation to explore whether all-trans retinoic acid (ATRA) could regulate the apoE expression in the pathological process of GS. 120 Wistar rats w...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2011.03.001
更新日期:2011-06-01 00:00:00
abstract:OBJECTIVE:The aim of the study is to determine the frequency and functionality of blood conventional dendritic cells (cDCs) in relation to disease activity in systemic lupus erythematosus. METHODS:Blood cDCs were enumerated for 34 SLE patients, defined as "active" (SLEDAI ≥ 4) or "inactive" (SLEDAI < 4), 26 RA subject...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2013.06.003
更新日期:2013-10-01 00:00:00
abstract::Lung Adenocarcinoma is one of the most leading causes of death worldwide. Early detection of this cancer could enhance the survival chance of patients and even lead to better and more effective treatment. One of the approaches to find out more about biological malfunctions is using "omics" data. Among diverse computat...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2019.104360
更新日期:2020-02-01 00:00:00
abstract::We tested the hypothesis that structural changes in the genome parallel age- and organ-specific phenotypes in conjunction with the differential transposition activities of retroelements. The genomes of the liver from C57BL/6J mice were larger than other organs, coinciding with an increase in genomic copies of certain ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2012.04.013
更新日期:2012-08-01 00:00:00
abstract::Currently there is critical need for the identification of reliable biomarkers to help guide clinical management of multiple sclerosis (MS) patients. We investigated the combined roles of Response Gene to Complement 32 (RGC-32), FasL, CDC2, AKT, and IL-21 as possible biomarkers of relapse and response to glatiramer ac...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2015.09.007
更新日期:2015-12-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(84)90003-0
更新日期:1984-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.yexmp.2019.104325
更新日期:2019-12-01 00:00:00
abstract::Meningiomas are among the most common tumors of the adult central nervous system (CNS). They are classified by the World Health Organization into three pathologic grades with increasing severity: grade I are benign with favorable treatment outcomes and low recurrence rates while grade III display malignant behavior an...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2018.10.007
更新日期:2018-12-01 00:00:00
abstract::The dialysate fluid of uremic patients exhibits, in vitro, an inhibitory effect on cell growth, owing to urea, guanidino compounds, and substances named middle molecules. The polyamines are compounds which exhibit high levels in biological fluids during either normal development or disease such as psoriasis, uremia, a...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1997.2215
更新日期:1997-01-01 00:00:00
abstract::A novel intrauterine transplantation (IUT) approach was developed to improve the efficiency of engraftment of hematopoietic stem cells (HSCs). HSCs with a green fluorescent protein (GFP) reporter gene were transplanted in utero on days 12.5, 13.5 and 14.5 post coitum (p.c.). The degree of chimerism of donor cells in r...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2009.07.009
更新日期:2009-12-01 00:00:00
abstract::Male Wistar rats were administered daily intraperitoneal injections of 125, 250, 500, and 1000 mg/kg/day of vitamin B6 for 2 and 6 weeks and the histogenesis of the testicular damage was investigated. A reduction of germ cells was not prominent in the 2-week groups, whereas a delay in spermiation, degeneration of elon...
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pub_type: 杂志文章
doi:10.1016/0014-4800(91)90019-t
更新日期:1991-08-01 00:00:00
abstract::In a previous study the authors demonstrated monotypic foci, all of timidus type, in the atherosclerotic lesions and normal aortic tissue of glucose-6-phosphate dehydrogenase (G-6-PD) mosaic hares fed 25-hydroxycholesterol or triol in addition to a high-cholesterol diet. Other hares fed the high-cholesterol diet witho...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(85)90019-x
更新日期:1985-02-01 00:00:00
abstract::Rheumatoid arthritis (RA) is an aggressive inflammatory disease in which chemokines are thought to recruit leukocytes and induce angiogenesis. The aim of this study was to investigate the effects of sulfasalazine (SASP) and its metabolites, sulfapyridine (SP), and 5-aminosalicylic acid (5ASA) on chemokine production b...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00