Abstract:
:This study aimed to investigate the effects of cyclopamine, a specific inhibitor of Hedgehog signaling pathways, on the chondrogenic differentiation of mesenchymal stem cells (MSCs). During culture, the experimental groups were treated with cyclopamine and their cell proliferation status was assessed using the MTT test. The extra-bone cellular matrix (ECM) and Collagen II (Col II) was detected by toluidine blue staining and immunohistochemistry of cells. The concentrations of Col II and aggrecan in the culture solution and cytosol were detected using ELISA on the 7th, 14th, and 21st days of cyclopamine induction. Gene and protein expression of Col II and aggrecan were analyzed on the 14th day of cyclopamine induction using real-time PCR and western blot analyses. No significant differences in proliferation of mesenchymal stem cells were found between the control group and the group treated with cyclopamine. Compared to the blank control group, the ECM level was low and the protein and mRNA concentrations of Collagen II (Col II) and aggrecan in the culture solution and cytosol, respectively, were significantly reduced in the experimental group. The Smo acted as a key point in the regulations of Hedgehog signaling pathway on the chondrogenic differentiation of rabbit MSCs.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Wu X,Cai ZD,Lou LM,Chen ZRdoi
10.3390/ijms14035966subject
Has Abstractpub_date
2013-03-14 00:00:00pages
5966-77issue
3issn
1422-0067pii
ijms14035966journal_volume
14pub_type
杂志文章abstract::In the last decade, a wide range of avian influenza viruses (AIVs) have infected various mammalian hosts and continuously threaten both human and animal health. It is a result of overcoming the inter-species barrier which is mostly associated with gene reassortment and accumulation of mutations in their gene segments....
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18122706
更新日期:2017-12-13 00:00:00
abstract::The translation of metallothioneins (MTs) is one of the defense strategies by which organisms protect themselves from metal-induced toxicity. MTs belong to a family of proteins comprising MT-1, MT-2, MT-3, and MT-4 classes, with multiple isoforms within each class. The main aim of this study was to determine the behav...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18030610
更新日期:2017-03-11 00:00:00
abstract::Background: We previously reported that the adipokine chemerin, when added exogenously to the isolated rat mesenteric artery, amplified electrical field-stimulated (EFS) contraction. The Chemerin1 antagonist CCX832 alone inhibited EFS-induced contraction in tissues with but not without perivascular adipose tissue (PVA...
journal_title:International journal of molecular sciences
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doi:10.3390/ijms21176392
更新日期:2020-09-02 00:00:00
abstract::Jasmonic acid (JA) carboxyl methyltransferase (JMT), a key enzyme in jasmonate-regulated plant responses, may be involved in plant defense and development by methylating JA to MeJA, thus influencing the concentrations of MeJA in plant. In this study, we isolated the JMT gene from Salvia miltiorrhiza, an important medi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19123788
更新日期:2018-11-28 00:00:00
abstract::A common subsequence is a fragment of the amino acid chain that occurs in more than one protein. Common subsequences may be an object of interest for food scientists as biologically active peptides, epitopes, and/or protein markers that are used in comparative proteomics. An individual bioactive fragment, in particula...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms160920748
更新日期:2015-09-01 00:00:00
abstract::Sarcopenia is primarily characterized by skeletal muscle disturbances such as loss of muscle mass, quality, strength, and physical performance. It is commonly seen in elderly patients with chronic diseases. The prevalence of sarcopenia in chronic heart failure (HF) patients amounts to up to 20% and may progress into c...
journal_title:International journal of molecular sciences
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更新日期:2020-09-08 00:00:00
abstract:BACKGROUND:The molecular signature of endometrial receptivity still remains barely understood, especially when focused on the possible benefit of therapeutical interventions and implantation-related pathologies. Therefore, the protein composition of tissue and isolated primary cells (endometrial stromal cells, ESCs) fr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20020425
更新日期:2019-01-19 00:00:00
abstract::The normal bone regeneration process is a complex and coordinated series of events involving different cell types and molecules. However, this process is impaired in critical-size/large bone defects, with non-unions or delayed unions remaining a major clinical problem. Novel strategies are needed to aid the current th...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
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更新日期:2019-07-12 00:00:00
abstract::B-Cell Lymphoma-extra-large (BCL-xL) is involved in longevity and successful aging, which indicates a role for BCL-xL in cell survival pathway regulation. Beyond its well described role as an inhibitor of apoptosis by preventing cytochrome c release, BCL-xL has also been related, indirectly, to autophagy and senescenc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
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更新日期:2020-01-09 00:00:00
abstract::Autophagy is an essential mechanism to maintain cellular homeostasis. Besides its role in controlling the quality of cytoplasmic components, it participates in nutrient obtaining and lipid mobilization under stressful conditions. Furthermore, autophagy is involved in the regulation of systemic metabolism as its blocka...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
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更新日期:2020-04-03 00:00:00
abstract::The objective of the present study is to determine whether methoxychlor (MXC) exposure in adulthood affects rat Leydig cell regeneration and to compare its effects with estradiol (E2). Adult 90-day-old male Sprague-Dawley rats received ethane dimethane sulfonate (EDS) to eliminate the adult Leydig cell population. Sub...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15057812
更新日期:2014-05-06 00:00:00
abstract::Marine environments harbor a wide range of microorganisms from the three domains of life. These microorganisms have great potential to enable discovery of new enzymes and bioactive compounds for industrial use. However, only ~1% of microorganisms from the environment can currently be identified through cultured isolat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms161226101
更新日期:2015-11-27 00:00:00
abstract:INTRODUCTION:Targeted agents such as bevacizumab (BEV) or poly (ADP-ribose) polymerase inhibitors (PARPi) which have been added as concomitant or maintenance therapies have been shown to improve progression-free survival (PFS) in patients with platinum-sensitive recurrent ovarian cancer (PS rOC). In the absence of dire...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21113805
更新日期:2020-05-27 00:00:00
abstract::Amyloidosis refers to aggregates of protein that accumulate and are deposited as amyloid fibrils into plaques. When these are detected in organs, they are the main hallmark of Alzheimer's disease, Parkinson's disease, and other related diseases. Recent medical advances have shown that many precursors and proteins can ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21061978
更新日期:2020-03-13 00:00:00
abstract::Complex neurodevelopmental syndromes frequently have an unknown etiology, in which genetic factors play a pathogenic role. This study utilizes whole-exome sequencing (WES) to examine four members of a family with a son presenting, since birth, with epileptic-like crises, combined with cerebral palsy, severe neuromotor...
journal_title:International journal of molecular sciences
pub_type:
doi:10.3390/ijms21124447
更新日期:2020-06-23 00:00:00
abstract::Reproductive dysfunction and malignancies related to the male gender represent a serious health concern, whose incidence has significantly risen over the past years. Prior to treatment, testicular or prostate cancer patients often display poor semen characteristics similar to subfertile or infertile patients. This fac...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms16047230
更新日期:2015-03-31 00:00:00
abstract::The coupling of alternative splicing with the nonsense-mediated decay (NMD) pathway maintains quality control of the transcriptome in eukaryotes by eliminating transcripts with premature termination codons (PTC) and fine-tunes gene expression. Long noncoding RNA (lncRNA) can regulate multiple cellular processes, inclu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165605
更新日期:2020-08-05 00:00:00
abstract::Hybrid peptides integrating different functional domains of peptides have many advantages, such as remarkable antimicrobial activity, lower hemolysis and ideal cell selectivity, compared with natural antimicrobial peptides. FV7 (FRIRVRV-NH₂), a consensus amphiphilic sequence was identified as being analogous to host d...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18020339
更新日期:2017-02-06 00:00:00
abstract::Expression of human granulocyte macrophage colony stimulating factor (hGMCSF), a cytokine of therapeutic importance, as a thioredoxin (TRX) fusion has been investigated in Escherichia coli BL21 (DE3) codon plus cells. The expression of this protein was low when cloned under the T7 promoter without any fusion tags. Hig...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12032064
更新日期:2011-01-01 00:00:00
abstract::Kidney renal cell carcinoma (KIRC), which is the most common subtype of kidney cancer, has a poor prognosis and a high mortality rate. In this study, a multi-omics analysis is performed to build a multi-gene prognosis signature for KIRC. A combination of a DNA methylation analysis and a gene expression data analysis r...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20225720
更新日期:2019-11-14 00:00:00
abstract::The role of dynamic processes on self-assembled structures of 4'-([2,2':6', 2''-terpyridin]-4'-yl)-[1,1'-biphenyl]-4-carboxylic acid (l) molecules on Au(III) has been studied by scanning tunneling microscopy. The as-deposited monolayer is closed-packed and periodic in a short-range due to dipole forces. A thermal anne...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms14035686
更新日期:2013-03-11 00:00:00
abstract::Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the struct...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19102912
更新日期:2018-09-25 00:00:00
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journal_title:International journal of molecular sciences
pub_type: 临床试验,杂志文章
doi:10.3390/ijms20194710
更新日期:2019-09-23 00:00:00
abstract::Cutaneous squamous cell carcinoma (CSCC) is the second most frequent cancer in humans and it can be locally invasive and metastatic to distant sites. MicroRNAs (miRNAs or miRs) are endogenous, small, non-coding RNAs of 19-25 nucleotides in length, that are involved in regulating gene expression at a post-transcription...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20092181
更新日期:2019-05-02 00:00:00
abstract::Epigenetics is defined as the heritable phenotypic changes which do not involve alterations in the DNA sequence, including histone modifications, non-coding RNAs, and DNA methylation. Recently, much attention has been paid to the role of hypoxia-mediated epigenetic regulation in cancer, pulmonary hypertension, adaptat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20122937
更新日期:2019-06-15 00:00:00
abstract::Land plants are anchored in one place for most of their life cycle and therefore must constantly adapt their growth and metabolism to abiotic stresses such as light intensity, temperature and the availability of water and essential minerals. Thus, plants' subsistence depends on their ability to regulate rapidly gene e...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms10073168
更新日期:2009-07-10 00:00:00
abstract::Pectin methylesterase inhibitor genes (PMEIs) are a large multigene family and play crucial roles in cell wall modifications in plant growth and development. Here, a comprehensive analysis of the PMEI gene family in Brassicacampestris, an important leaf vegetable, was performed. We identified 100 BrassicacampestrisPME...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19051338
更新日期:2018-05-02 00:00:00
abstract::Monoclonal antibody G250 (mAbG250) recognizes a determinant on carbonic anhydrase IX (CAIX). CAIX is expressed by virtually all renal cell carcinomas of the clear cell type (ccRCC), but expression in normal tissues is restricted. The homogeneous CAIX expression in ccRCC and excellent targeting capability of mAbG250 in...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms140611402
更新日期:2013-05-29 00:00:00
abstract::Melatonin has long been recognized as a positive signaling molecule and potent antioxidant in plants, which alleviates damage caused by adverse conditions such as salt, cold, and heat stress. In this study, we found a paradoxical role for melatonin in abiotic stress responses. Suppression of the serotonin N-acetyltran...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20205173
更新日期:2019-10-18 00:00:00
abstract::Parkinson's disease (PD) is a complex disorder underpinned by both environmental and genetic factors. The latter only began to be understood around two decades ago, but since then great inroads have rapidly been made into deconvoluting the genetic component of PD. In particular, recent large-scale projects such as gen...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21197332
更新日期:2020-10-04 00:00:00