Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture.

Abstract:

:Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the structural properties of the scaffolds (tissue matrix scaffold, TMS), and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between different scaffolding compartments, and in vivo tumor formation. This model system provides an additional option for studying human breast diseases such as breast cancer.

journal_name

Int J Mol Sci

authors

Rijal G,Wang J,Yu I,Gang DR,Chen RK,Li W

doi

10.3390/ijms19102912

subject

Has Abstract

pub_date

2018-09-25 00:00:00

issue

10

issn

1422-0067

pii

ijms19102912

journal_volume

19

pub_type

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