Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture.


:Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the structural properties of the scaffolds (tissue matrix scaffold, TMS), and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between different scaffolding compartments, and in vivo tumor formation. This model system provides an additional option for studying human breast diseases such as breast cancer.


Int J Mol Sci


Rijal G,Wang J,Yu I,Gang DR,Chen RK,Li W




Has Abstract


2018-09-25 00:00:00










  • Molecular mechanisms of host cytoskeletal rearrangements by Shigella invasins.

    abstract::Pathogen-induced reorganization of the host cell cytoskeleton is a common strategy utilized in host cell invasion by many facultative intracellular bacteria, such as Shigella, Listeria, enteroinvasive E. coli and Salmonella. Shigella is an enteroinvasive intracellular pathogen that preferentially infects human epithel...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Lee JH,Park H,Park YH

    更新日期:2014-10-10 00:00:00

  • The Novel HDAC8 Inhibitor WK2-16 Attenuates Lipopolysaccharide-Activated Matrix Metalloproteinase-9 Expression in Human Monocytic Cells and Improves Hypercytokinemia In Vivo.

    abstract::Dysregulated human monocytes/macrophages can synthesize and secrete matrix metalloproteinases (MMPs), which play important roles in the progression of sepsis. In this study, we investigated the effects and mechanism of a novel histone deacetylase (HDAC8) inhibitor, (E)-N-hydroxy-4-methoxy-2-(biphenyl-4-yl)cinnamide (W...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Jan JS,Chou YC,Cheng YW,Chen CK,Huang WJ,Hsiao G

    更新日期:2017-06-29 00:00:00

  • Downregulation of FOXP1 Inhibits Cell Proliferation in Hepatocellular Carcinoma by Inducing G1/S Phase Cell Cycle Arrest.

    abstract::Forkhead box P1 (FOXP1) belongs to a family of winged-helix transcription factors that are involved in the processes of cellular proliferation, differentiation, metabolism, and longevity. FOXP1 can affect cell proliferation and migratory ability in hepatocellular carcinoma (HCC) in vitro. However, little is known abou...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Wang X,Sun J,Cui M,Zhao F,Ge C,Chen T,Yao M,Li J

    更新日期:2016-09-08 00:00:00

  • Genome-wide investigation of multifocal and unifocal prostate cancer-are they genetically different?

    abstract::Prostate cancer is widely observed to be biologically heterogeneous. Its heterogeneity is manifested histologically as multifocal prostate cancer, which is observed more frequently than unifocal prostate cancer. The clinical and prognostic significance of either focal cancer type is not fully established. To investiga...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ibeawuchi C,Schmidt H,Voss R,Titze U,Abbas M,Neumann J,Eltze E,Hoogland AM,Jenster G,Brandt B,Semjonow A

    更新日期:2013-06-03 00:00:00

  • Characterization of Micro-RNA Changes during the Progression of Type 2 Diabetes in Zucker Diabetic Fatty Rats.

    abstract::The aim of the present pilot study was the identification of micro-RNA changes over time during the development and progression of type 2 diabetes (T2D) in Zucker diabetic fatty rats (ZDF rats). T2D is a complex metabolic disorder that is characterized, inter alia, by progressive failure of pancreatic β cells to produ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Delic D,Eisele C,Schmid R,Luippold G,Mayoux E,Grempler R

    更新日期:2016-05-03 00:00:00

  • Restraint Stress in Mice Alters Set of 25 miRNAs Which Regulate Stress- and Depression-Related mRNAs.

    abstract::In the present study, we aim to identify the effect of restrain stress (RS) on the expression of miRNAs in mouse serum. We used three genotypes of animals (mice with knock-out of the gene-encoding norepinephrine transporter, NET-KO; C57BL/6J, and SWR/J) which had previously been shown to display different sensitivity ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Solich J,Kuśmider M,Faron-Górecka A,Pabian P,Dziedzicka-Wasylewska M

    更新日期:2020-12-12 00:00:00

  • An Integrated Approach of Proteomics and Computational Genetic Modification Effectiveness Analysis to Uncover the Mechanisms of Flood Tolerance in Soybeans.

    abstract::Flooding negatively affects the growth of soybeans. Recently, omic approaches have been used to study abiotic stress responses in plants. To explore flood-tolerant genes in soybeans, an integrated approach of proteomics and computational genetic modification effectiveness analysis was applied to the soybean (Glycine m...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Wang X,Sakata K,Komatsu S

    更新日期:2018-04-26 00:00:00

  • Constitutive Differential Features of Type 2 Transglutaminase in Cells Derived from Celiac Patients and from Healthy Subjects.

    abstract::Type 2 transglutaminase (TG2) is a ubiquitous enzyme able to modify gliadin peptides introduced into the organism through the diet. By means of its catalytic activity, TG2 seems to have an important pathogenetic role in celiac disease (CD), an inflammatory intestinal disease caused by the ingestion of gluten-containin...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Paolella G,Nanayakkara M,Sposito S,Lepretti M,Auricchio S,Esposito C,Barone MV,Martucciello S,Caputo I

    更新日期:2020-02-12 00:00:00

  • A Novel Mechanism of S-equol Action in Neurons and Astrocytes: The Possible Involvement of GPR30/GPER1.

    abstract::S-equol is a major bacterial metabolite of the soy isoflavone daidzein. It is known to be a phytoestrogen that acts by binding to the nuclear estrogen receptors (ERs) that are expressed in various brain regions, including the cerebellum. However, the effects of S-equol on cerebellar development and function have not y...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ariyani W,Miyazaki W,Koibuchi N

    更新日期:2019-10-18 00:00:00

  • Growth promotion of Yunnan Pine early seedlings in response to foliar application of IAA and IBA.

    abstract::A field experiment was conducted using a 3 × 3 orthogonal regression design to explore the growth promotion of one-year-old Yunnan pine seedlings (Pinus yunnanensis Franch.) in response to foliar application of IAA (indole-3-acetic acid) at rates of 0, 200 and 400 mg·L(-1) and IBA (indole-3-butyric acid) at rates of 0...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Xu Y,Zhang Y,Li Y,Li G,Liu D,Zhao M,Cai N

    更新日期:2012-01-01 00:00:00

  • Optimization and evaluation of magnetic bead separation combined with matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS) for proteins profiling of peritoneal dialysis effluent.

    abstract::Peritoneal dialysis effluent (PDE) potentially carries an archive of peptides relevant to pathological processes in abdominal and surrounding tissues. Magnetic beads and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is one such approach that offers a unique tool for profiling of peptides...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Guo N,Wen Q,Li ZJ,Xu RC,Peng FF,Yu XQ

    更新日期:2014-01-16 00:00:00

  • PD-1 and PD-L1 Expression on Circulating Lymphocytes as a Marker of Epstein-Barr Virus Reactivation-Associated Proliferative Glomerulonephritis.

    abstract::Alterations to the programmed cell death protein-1 (PD-1) pathway were previously shown to be involved in a poorer prognosis for patients with proliferative glomerulonephritis (PGN). Here, we investigated the association between several infectious agents and the expression of PD-1 and its ligand (PD-L1) on T and B lym...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Grywalska E,Smarz-Widelska I,Korona-Głowniak I,Mertowski S,Gosik K,Hymos A,Ludian J,Niedźwiedzka-Rystwej P,Roliński J,Załuska W

    更新日期:2020-10-27 00:00:00

  • The Molecular Mechanisms of Adaptive Response Related to Environmental Stress.

    abstract::The exposure of living organisms to environmental stress triggers defensive responses resulting in the activation of protective processes. Whenever the exposure occurs at low doses, defensive effects overwhelm the adverse effects of the exposure; this adaptive situation is referred to as "hormesis". Environmental, phy...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Rossnerova A,Izzotti A,Pulliero A,Bast A,Rattan SIS,Rossner P

    更新日期:2020-09-25 00:00:00

  • Preparation of Biodegradable and Elastic Poly(ε-caprolactone-co-lactide) Copolymers and Evaluation as a Localized and Sustained Drug Delivery Carrier.

    abstract::To develop a biodegradable polymer possessing elasticity and flexibility, we synthesized MPEG-b-(PCL-co-PLA) copolymers (PC x L y A), which display specific rates of flexibility and elasticity. We synthesize the PC ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Park JH,Lee BK,Park SH,Kim MG,Lee JW,Lee HY,Lee HB,Kim JH,Kim MS

    更新日期:2017-03-21 00:00:00

  • Cherry Extract from Prunus avium L. to Improve the Resistance of Endothelial Cells to Oxidative Stress: Mucoadhesive Chitosan vs. Poly(lactic-co-glycolic acid) Nanoparticles.

    abstract::Polyphenolic compounds contained in cherry extract (CE) are well known for their antioxidant and anti-inflammatory properties. Unfortunately, most of these natural compounds have low oral bioavailability, reducing their widespread use. Here, different concentrations of polyphenol-rich CE from Tuscany (Italy), encapsul...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Beconcini D,Fabiano A,Di Stefano R,Macedo MH,Felice F,Zambito Y,Sarmento B

    更新日期:2019-04-10 00:00:00

  • A Genome-Wide Methylation Approach Identifies a New Hypermethylated Gene Panel in Ulcerative Colitis.

    abstract::The cause of inflammatory bowel disease (IBD) is still unknown, but there is growing evidence that environmental factors such as epigenetic changes can contribute to the disease etiology. The aim of this study was to identify newly hypermethylated genes in ulcerative colitis (UC) using a genome-wide DNA methylation ap...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Kang K,Bae JH,Han K,Kim ES,Kim TO,Yi JM

    更新日期:2016-08-09 00:00:00

  • Correction: Marcuzzi, E., et al. Chemokines and Chemokine Receptors: Orchestrating Tumor Metastasization. Int. J. Mol. Sci. 2019, 20, 96.

    abstract::Due to a typesetting error during layout, several references were incorrectly listed in [...]. ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,已发布勘误


    authors: Marcuzzi E,Angioni R,Molon B,Calì B

    更新日期:2019-05-29 00:00:00

  • Histone Lysine Methylation and Neurodevelopmental Disorders.

    abstract::Methylation of several lysine residues of histones is a crucial mechanism for relatively long-term regulation of genomic activity. Recent molecular biological studies have demonstrated that the function of histone methylation is more diverse and complex than previously thought. Moreover, studies using newly available ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Kim JH,Lee JH,Lee IS,Lee SB,Cho KS

    更新日期:2017-06-30 00:00:00

  • Translocator Protein (TSPO) as a Potential Biomarker in Human Cancers.

    abstract::TSPO is a receptor involved in the regulation of cellular proliferation, apoptosis and mitochondrial functions. Previous studies showed that the expression of TSPO protein correlated positively with tumour malignancy and negatively with patient survival. The aim of this study was to determine the transcription of Tspo...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Bhoola NH,Mbita Z,Hull R,Dlamini Z

    更新日期:2018-07-25 00:00:00

  • De novo Transcriptome Generation and Annotation for Two Korean Endemic Land Snails, Aegista chejuensis and Aegista quelpartensis, Using Illumina Paired-End Sequencing Technology.

    abstract::Aegista chejuensis and Aegista quelpartensis (Family-Bradybaenidae) are endemic to Korea, and are considered vulnerable due to declines in their population. The limited genetic resources for these species restricts the ability to prioritize conservation efforts. We sequenced the transcriptomes of these species using I...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Kang SW,Patnaik BB,Hwang HJ,Park SY,Wang TH,Park EB,Chung JM,Song DK,Patnaik HH,Lee JB,Kim C,Kim S,Park HS,Lee JS,Han YS,Lee YS

    更新日期:2016-03-15 00:00:00

  • Advantages and Pitfalls of Mass Spectrometry Based Metabolome Profiling in Systems Biology.

    abstract::Mass spectrometry-based metabolome profiling became the method of choice in systems biology approaches and aims to enhance biological understanding of complex biological systems. Genomics, transcriptomics, and proteomics are well established technologies and are commonly used by many scientists. In comparison, metabol...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Aretz I,Meierhofer D

    更新日期:2016-04-27 00:00:00

  • Unravelling genes and pathways implicated in working memory of schizophrenia in Han Chinese.

    abstract::Working memory deficit is the core neurocognitive disorder in schizophrenia patients. To identify the factors underlying working memory deficit in schizophrenia patients and to explore the implication of possible genes in the working memory using genome-wide association study (GWAS) of schizophrenia, computerized dela...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ren H,Zhang C,Huang C,Li N,Li M,Li Y,Deng W,Ma X,Xiang B,Wang Q,Li T

    更新日期:2015-01-20 00:00:00

  • Perillyl Alcohol and Its Drug-Conjugated Derivatives as Potential Novel Methods of Treating Brain Metastases.

    abstract::Metastasis to the central nervous system remains difficult to treat, and such patients are faced with a dismal prognosis. The blood-brain barrier (BBB), despite being partially compromised within malignant lesions in the brain, still retains much of its barrier function and prevents most chemotherapeutic agents from e...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Chen TC,Da Fonseca CO,Schönthal AH

    更新日期:2016-09-02 00:00:00

  • Ca2+/Calmodulin-Dependent AtSR1/CAMTA3 Plays Critical Roles in Balancing Plant Growth and Immunity.

    abstract::During plant-pathogen interactions, plants have to relocate their resources including energy to defend invading organisms; as a result, plant growth and development are usually reduced. Arabidopsis signal responsive1 (AtSR1) has been documented as a negative regulator of plant immune responses and could serve as a pos...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Yuan P,Du L,Poovaiah BW

    更新日期:2018-06-14 00:00:00

  • Translational Regenerative Therapies for Chronic Spinal Cord Injury.

    abstract::Spinal cord injury is a chronic and debilitating neurological condition that is currently being managed symptomatically with no real therapeutic strategies available. Even though there is no consensus on the best time to start interventions, the chronic phase is definitely the most stable target in order to determine ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Dalamagkas K,Tsintou M,Seifalian A,Seifalian AM

    更新日期:2018-06-15 00:00:00

  • Controlling J-Aggregates Formation and Chirality Induction through Demetallation of a Zinc(II) Water Soluble Porphyrin.

    abstract::Under acidic conditions and at high ionic strength, the zinc cation is removed from its metal complex with 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin (TPPS4) thus leading to the diacid free porphyrin, that subsequently self-organize into J-aggregates. The kinetics of the demetallation step and the successive supr...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Occhiuto IG,Castriciano MA,Trapani M,Zagami R,Romeo A,Pasternack RF,Monsù Scolaro L

    更新日期:2020-06-03 00:00:00

  • The photodynamic antibacterial effects of silicon phthalocyanine (Pc) 4.

    abstract::The emergence of antibiotic-resistant strains in facultative anaerobic Gram-positive coccal bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), is a global health issue. Typically, MRSA strains are found associated with institutions like hospitals but recent data suggest that they are becoming more p...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Dimaano ML,Rozario C,Nerandzic MM,Donskey CJ,Lam M,Baron ED

    更新日期:2015-04-08 00:00:00

  • Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus Bordetella Analyzed Exclusively by NMR Spectroscopy.

    abstract::Bacterial pathogens expose on the cell surface a variety of complex carbohydrate molecules. Gram-negative bacteria produce lipopolysaccharides, which are the main components of the outer membrane of bacterial envelopes and play a major role in host-pathogen interactions. B. pertussis, B. parapertussis, B. bronchisepti...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ucieklak K,Koj S,Niedziela T

    更新日期:2021-01-21 00:00:00

  • Impact and Intricacies of Bone Marrow Microenvironment in B-cell Lymphomas: From Biology to Therapy.

    abstract::Lymphoma, a group of widely prevalent hematological malignancies of lymphocyte origin, has become the focus of significant clinical research due to their high propensity for refractory/relapsed (R/R) disease, leading to poor prognostic outcomes. The complex molecular circuitry in lymphomas, especially in the aggressiv...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Sircar A,Chowdhury SM,Hart A,Bell WC,Singh S,Sehgal L,Epperla N

    更新日期:2020-01-30 00:00:00

  • Urinary Metabolic Phenotyping Reveals Differences in the Metabolic Status of Healthy and Inflammatory Bowel Disease (IBD) Children in Relation to Growth and Disease Activity.

    abstract:BACKGROUND:Growth failure and delayed puberty are well known features of children and adolescents with inflammatory bowel disease (IBD), in addition to the chronic course of the disease. Urinary metabonomics was applied in order to better understand metabolic changes between healthy and IBD children. METHODS:21 Pediat...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Martin FP,Ezri J,Cominetti O,Da Silva L,Kussmann M,Godin JP,Nydegger A

    更新日期:2016-08-11 00:00:00