Abstract：
:The environment surrounding chondrocytes changes drastically in osteoarthritis (OA). For instance, the osmolarity in cartilage (ranging from 350 to 460 mOsm in healthy tissue) decreases during the progression of OA, reaching 270 mOsm. The objective of this study was to evaluate how osmolarity influences human OA chondrocytes. For this purpose, the osmolarity of the culture medium (340 mOsm) was increased to 380, 420 or 460 mOsm and its effect on the phenotype, matrix production, protease expression, cytokine release and growth and differentiation factor-5 (GDF-5) receptor expression in human OA chondrocytes was evaluated in a monolayer. Afterwards, the same parameters, as well as the responsiveness to GDF-5, were evaluated in 3D culture at 340 and 380 mOsm. Our results revealed that increasing the medium osmolarity increased matrix production but also reduced cytokine release, type I collagen and protease expression. It was also demonstrated that at 380 mOsm, the response to GDF-5 in 3D culture was more robust than at 340 mOsm. For the first time, it was established that a decreased osmolarity plays a role in sustaining inflammation and catabolic activities in OA chondrocytes and decreases their responsiveness to GDF-5. This indicates that osmolarity is a critical aspect of OA pathobiology.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Mang T,Lindemann S,Gigout Adoi
10.3390/ijms21020531subject
Has Abstractpub_date
2020-01-14 00:00:00issue
2issn
1422-0067pii
ijms21020531journal_volume
21pub_type
杂志文章abstract：:Obesity is a worldwide epidemic problem and correlates to varieties of acute or chronic lung diseases such as acute respiratory distress syndrome, chronic obstructive pulmonary disease, and pulmonary fibrosis. An increase of leptin, a kind of adipokine, in lean mice plasma has been found to impair immune responses and...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051078
更新日期：2019-03-02 00:00:00
abstract：:Understanding the kinetics of peptide self-assembly is important because of the involvement of peptide amyloid fibrils in several neurodegenerative diseases. In this paper, we have studied the dissolution kinetics of self-assembled model peptide fibrils after a dilution quench. Due to the low concentrations involved, ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21207671
更新日期：2020-10-16 00:00:00
abstract：:Aromatic isocyanides including isocyanonaphthalene derivatives have been proven to be very effective fluorescent sensors for the quantification of Hg(II) ions in water. Thus, the reaction of 1,5-isocyanoaminonaphthalene (1,5-ICAN), which is one of the most important members of this family, with water and HgCl2 as the ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21155588
更新日期：2020-08-04 00:00:00
abstract：:Currently, the majority of prebiotics in the market are derived from non-digestible oligosaccharides. Very few studies have focused on non-digestible long chain complex polysaccharides in relation to their potential as novel prebiotics. Cereals β-glucans have been investigated for immune-modulating properties and bene...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15023025
更新日期：2014-02-20 00:00:00
abstract：:Metal compounds such as arsenic, cadmium, chromium, cobalt, lead, mercury, and nickel are classified as carcinogens affecting human health through occupational and environmental exposure. However, the underlying mechanisms involved in tumor formation are not well clarified. Interference of metal homeostasis may result...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms12129576
更新日期：2011-01-01 00:00:00
abstract：:Methyl CpG binding protein-2 (MeCP2) isoforms (E1 and E2) are important epigenetic regulators in brain cells. Accordingly, MeCP2 loss- or gain-of-function mutation causes neurodevelopmental disorders, including Rett syndrome (RTT), MECP2 duplication syndrome (MDS), and autism spectrum disorders (ASD). Within different...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20081845
更新日期：2019-04-14 00:00:00
abstract：:Present day awareness of biofilm colonization on polymeric surfaces has prompted the scientific community to develop an ever-increasing number of new materials with anti-biofilm features. However, compared to the large amount of work put into discovering potent biofilm inhibitors, only a small number of papers deal wi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20153794
更新日期：2019-08-03 00:00:00
abstract：:: Mitochondrial respiratory chain supercomplexes (RCS), particularly, the respirasome, which contains complexes I, III, and IV, have been suggested to participate in facilitating electron transport, reducing the production of reactive oxygen species (ROS), and maintaining the structural integrity of individual electro...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051555
更新日期：2020-02-25 00:00:00
abstract：:Diabetes is associated with a two to three-fold increase in risk of cardiovascular disease. However, intensive glucose-lowering therapy aiming at reducing HbA1c to a near-normal level failed to suppress cardiovascular events in recent randomized controlled trials. HbA1c reflects average glucose level rather than glyce...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms151018381
更新日期：2014-10-13 00:00:00
abstract：:In our ongoing research to discover natural products with neuroprotective effects, hyperoside (quercetin 3-O-galactoside) was isolated from Acer tegmentosum, which has been used in Korean traditional medicine to treat liver-related disorders. Here, we demonstrated that hyperoside protects cultured dopaminergic neurons...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20235832
更新日期：2019-11-20 00:00:00
abstract：:In systemic sclerosis (SSc), the possible involvement of lymphatic microcirculation and lymphangiogenesis has traditionally been overshadowed by the greater emphasis placed on dysfunctional blood vascular system and angiogenesis. In the present in vitro study, we explore for the first time whether the SSc microenviron...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20246189
更新日期：2019-12-07 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is a deadly form of liver malignancy with limited treatment options. Amplification and/or overexpression of c-MYC is one of the most frequent genetic events in human HCC. The mammalian target of Rapamycin Complex 1 (mTORC1) is a major functional axis regulating various aspects of cellula...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228467
更新日期：2020-11-11 00:00:00
abstract：:Tissues in the body are hierarchically structured composite materials with tissue-specific properties. Urea self-assembles via hydrogen bonding interactions into crystalline supracolloidal assemblies that can be used to impart macroscopic pores to polymer-based tissue scaffolds. In this communication, we explain the s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160920511
更新日期：2015-08-28 00:00:00
abstract：:Multiple sclerosis (MS) is a devastating neurological disease that predominantly affects young adults resulting in severe personal and economic impact. The majority of therapies for this disease were developed in, or are beneficial in experimental autoimmune encephalomyelitis (EAE), the animal model of MS. While known...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms14010547
更新日期：2012-12-27 00:00:00
abstract：:A fundamental step for cell growth and differentiation is the cell adhesion. The purpose of this study was to determine the adhesion of different cell lineages, adipose derived stromal cells, osteoblasts, and gingival fibroblast to titanium and zirconia dental implants with different surface treatments. Primary cells ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21114110
更新日期：2020-06-09 00:00:00
abstract：:Herein, we propose newly designed and synthesized gold nanopeanuts (Au NPes) as supports for cisplatin (cPt) immobilization, dedicated to combined glioblastoma nano-chemo-radiotherapy. Au NPes offer a large active surface, which can be used for drugs immobilization. Transmission electron microscopy (TEM) revealed that...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21239082
更新日期：2020-11-29 00:00:00
abstract：:Studies conducted on Zebrafish embryos in our laboratory have allowed for the identification of precise moments of organogenesis in which a lot of genes are switched on and off, a sign that the genome is undergoing substantial changes in gene expression. Stem cell growth and differentiation stage-factors present in di...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21144914
更新日期：2020-07-12 00:00:00
abstract：:Development of stimuli-responsive supramolecular micelles that enable high levels of well-controlled drug release in cancer cells remains a grand challenge. Here, we encapsulated the antitumor drug doxorubicin (DOX) and pro-photosensitizer 5-aminolevulinic acid (5-ALA) within adenine-functionalized supramolecular mice...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22010154
更新日期：2020-12-25 00:00:00
abstract：:Metabolic stability is an important parameter to be optimized during the complex process of designing new active compounds. Tuning this parameter with the simultaneous maintenance of a desired compound's activity is not an easy task due to the extreme complexity of metabolic pathways in living organisms. In this study...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19041040
更新日期：2018-03-30 00:00:00
abstract：:Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells. Extracellular polysaccharides play an important role in matrix setup and maintenance. A water-insoluble polysaccharide was isolated and p...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051702
更新日期：2020-03-02 00:00:00
abstract：:Levodopa (LD) is used to increase dopamine level for treating Parkinson's disease. The major metabolism of LD to produce dopamine is decarboxylation. In order to understand the metabolism of LD; the electronic structure of levodopa was investigated at the Density Functional DFT/B3LYP level of theory using the 6-311+G*...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13044321
更新日期：2012-01-01 00:00:00
abstract：:Cannabinoids, the biologically active constituents of Cannabis, have potent neuronal and immunological effects. However, the basic and medical research dedicated to medical cannabis and cannabinoids is limited. The influence of these treatments on hematologic reconstitution and on the development of graft versus host ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20030668
更新日期：2019-02-04 00:00:00
abstract：:Nitric oxide (NO/cyclic guanosine monophosphate (cGMP)-regulated cellular mechanisms are involved in a variety of (patho-) physiological processes. One of the main effector molecules in this system, proteinkinase G (PKG), serves as a molecular switch by phosphorylating different target proteins and thereby turning the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19041180
更新日期：2018-04-12 00:00:00
abstract：:Depressive disorders and alcohol use disorders are widespread among the general population and are significant public health and economic burdens. Alcohol use disorders often co-occur with other psychiatric conditions and this dual diagnosis is called comorbidity. Depressive disorders invariably contribute to the deve...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21218123
更新日期：2020-10-30 00:00:00
abstract：:Normal tissue toxicity is a dose-limiting factor in radiation therapy. Therefore, a detailed understanding of the normal tissue response to radiation is necessary to predict the risk of normal tissue toxicity and to development strategies for tissue protection. One component of normal tissue that is continuously expos...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21072336
更新日期：2020-03-27 00:00:00
abstract：:Plants infected with DNA viruses produce massive quantities of virus-derived, 24-nucleotide short interfering RNAs (siRNAs), which can potentially direct viral DNA methylation and transcriptional silencing. However, growing evidence indicates that the circular double-stranded DNA accumulating in the nucleus for Pol II...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms140815233
更新日期：2013-07-24 00:00:00
abstract：:Whether or how neural stem cells (NSCs) respond to toll-like receptor 4 (TLR4) in an inflammatory environment caused by traumatic brain injury (TBI) has not been understood. In the present study, association between TLR4 expression and NSCs proliferation in the hippocampus was investigated in a mouse model of TBI usin...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150712651
更新日期：2014-07-17 00:00:00
abstract：:Heat shock proteins (Hsp) are a diverse group of constitutive and/or stress-induced molecules that are categorized into several classes on the basis of their molecular weight. Mammalian Hsp have been mostly regarded as intracellular chaperones that mediate a range of essential cellular functions, including proper fold...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21155298
更新日期：2020-07-26 00:00:00
abstract：:Invasive species represent an increasing threat to native ecosystems, harming indigenous taxa through predation, habitat modification, cross-species hybridization and alteration of ecosystem processes. Additionally, high economic costs are associated with environmental damage, restoration and control measures. The Bur...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14034793
更新日期：2013-02-28 00:00:00
abstract：:The bHLH-PAS (basic helix-loop-helix/ Period-ARNT-Single minded) proteins are a family of transcriptional regulators commonly occurring in living organisms. bHLH-PAS members act as intracellular and extracellular "signals" sensors, initiating response to endo- and exogenous signals, including toxins, redox potential, ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20194746
更新日期：2019-09-24 00:00:00
