Abstract：
:Invasive species represent an increasing threat to native ecosystems, harming indigenous taxa through predation, habitat modification, cross-species hybridization and alteration of ecosystem processes. Additionally, high economic costs are associated with environmental damage, restoration and control measures. The Burmese python, Python molurus bivittatus, is one of the most notable invasive species in the US, due to the threat it poses to imperiled species and the Greater Everglades ecosystem. To address population structure and relatedness, next generation sequencing was used to rapidly produce species-specific microsatellite loci. The Roche 454 GS-FLX Titanium platform provided 6616 di-, tri- and tetra-nucleotide repeats in 117,516 sequences. Using stringent criteria, 24 of 26 selected tri- and tetra-nucleotide loci were polymerase chain reaction (PCR) amplified and 18 were polymorphic. An additional six cross-species loci were amplified, and the resulting 24 loci were incorporated into eight PCR multiplexes. Multi-locus genotypes yielded an average of 61% (39%-77%) heterozygosity and 3.7 (2-6) alleles per locus. Population-level studies using the developed microsatellites will track the invasion front and monitor population-suppression dynamics. Additionally, cross-species amplification was detected in the invasive Ball, P. regius, and Northern African python, P. sebae. These markers can be used to address the hybridization potential of Burmese pythons and the larger, more aggressive P. sebae.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Hunter ME,Hart KMdoi
10.3390/ijms14034793subject
Has Abstractpub_date
2013-02-28 00:00:00pages
4793-804issue
3issn
1422-0067pii
ijms14034793journal_volume
14pub_type
杂志文章abstract：:Microalgae are unicellular photosynthetic organisms considered as potential alternative sources for biomass, biofuels or high value products. However, limited biomass productivity is commonly experienced in their cultivating system despite their high potential. One of the reasons for this limitation is the high therma...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19010155
更新日期：2018-01-05 00:00:00
abstract：:Advanced preservation injury (PI) after intestinal transplantation has deleterious short- and long-term effects and constitutes a major research topic. Logistics and costs favor rodent studies, whereas clinical translation mandates studies in larger animals or using human material. Despite diverging reports, no direct...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20133135
更新日期：2019-06-27 00:00:00
abstract：:The presence of abnormal ovarian ratios of myo-inositol (MI) to D-chiro-inositol (DCI) is a recurrent feature in PCOS. Available evidence suggests that MI and DCI may modulate steroid biosynthesis, likely in an opposite manner. Specifically, MI seems to induce estrogen production, while DCI has a role in the synthesis...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21197157
更新日期：2020-09-28 00:00:00
abstract：:Mammalian spermatozoa are infertile immediately after ejaculation and need to undergo a functional maturation process to acquire the competence to fertilize the female egg. During this process, called capacitation, the actin cytoskeleton dramatically changes its organization. First, actin fibers polymerize, forming a ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20174236
更新日期：2019-08-29 00:00:00
abstract：:In the present work, we investigated the effect of non-steroidal anti-inflammatory drugs (NSAIDs) on the monophenolase and diphenolase activity of mushroom tyrosinase. The results showed that diflunisal and indomethacin inhibited both monophenolase and diphenolase activity. For monophenolase activity, the lag time was...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12063998
更新日期：2011-01-01 00:00:00
abstract：:The chloromethyl-functionalized polystyrene is the most commonly used ammonium cation precursor for making anion exchange resins (AER) and membranes (AEM). However, the chloromethylation of polystyrene or styrene involves highly toxic and carcinogenic raw materials (e.g., chloromethyl ether) and the resultant ammonium...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249404
更新日期：2020-12-10 00:00:00
abstract：:Phospholipidosis (PLD), the intracellular accumulation of phospholipids, is an adaptive response to toxic stimuli and serves as an important parameter in the biological assessment of compounds. Cationic amphiphilic drugs are the main inducers of PLD and may impair the function of alveolar macrophages. In vivo and in v...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21218391
更新日期：2020-11-09 00:00:00
abstract：:Quantification of gene expression is crucial to connect genome sequences with phenotypic and physiological data. RNA-Sequencing (RNA-Seq) has taken a prominent role in the study of transcriptomic reactions of plants to various environmental and genetic perturbations. However, comparative tests of different tools for R...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051720
更新日期：2020-03-03 00:00:00
abstract：:Smart or stimuli-responsive materials are an emerging class of materials used for tissue engineering and drug delivery. A variety of stimuli (including temperature, pH, redox-state, light, and magnet fields) are being investigated for their potential to change a material's properties, interactions, structure, and/or d...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21134724
更新日期：2020-07-02 00:00:00
abstract：:In a previous phylogenetic study of the family of pyruvate kinase, we found one cluster with Glu117 and another with Lys117. Those sequences with Glu117 have Thr113 and are K+-dependent, whereas those with Lys117 have Leu113 and are K+-independent. The carbonyl oxygen of Thr113 is one of the residues that coordinate K...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms151222214
更新日期：2014-12-02 00:00:00
abstract：:Fumarylacetoacetate hydrolase (FAH) is the last enzyme in the degradation pathway of the amino acids tyrosine and phenylalanine in mammals that catalyzes the hydrolysis of 4-fumarylacetoacetate into acetoacetate and fumarate. Mutations of the FAH gene are associated with hereditary tyrosinemia type I (HT1), resulting ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228719
更新日期：2020-11-18 00:00:00
abstract：:A comprehensive proteome map of T-lymphoblastic leukemia cells and its alterations after daunorubicin, doxorubicin and mitoxantrone treatments was monitored and evaluated either by paired comparison with relevant untreated control and using multivariate classification of treated and untreated samples. With the main fo...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms131215536
更新日期：2012-11-22 00:00:00
abstract：:Low-grade inflammation is implicated in the pathogenesis of atherosclerosis, metabolic syndrome, and apathy as a form of vascular depression. We analyzed the brain magnetic resonance imaging findings in 259 community-dwelling older adults (122 men and 137 women, with a mean age of 68.4 years). The serum concentrations...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20081905
更新日期：2019-04-17 00:00:00
abstract：:Mitochondria have emerged as key players regarding cellular homeostasis not only due to their contribution regarding energy production through oxidative phosphorylation, but also due to their involvement in signaling, ion regulation, and programmed cell death. Indeed, current knowledge supports the notion that mitocho...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18081821
更新日期：2017-08-22 00:00:00
abstract：:The use of herbicides is an effective and economic way to control weeds, but their availability for rapeseed is limited due to the shortage of herbicide-resistant cultivars in China. The single-point mutation in the acetohydroxyacidsynthase (AHAS) gene can lead to AHAS-inhibiting herbicide resistance. In this study, t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041345
更新日期：2020-02-17 00:00:00
abstract：:Squamous cell carcinoma (SCC) is the second commonest type of skin cancer, and SCCs make up about 90% of head and neck cancers (HNSCCs). HNSCCs harbor two frequent molecular alterations, namely, gain-of-function alterations of phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) and loss-of-...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21186585
更新日期：2020-09-09 00:00:00
abstract：:Nicotine causes tobacco dependence, which may result in fatal respiratory diseases. The striatum is a key structure of forebrain basal nuclei associated with nicotine dependence. In the striatum, glutamate release is increased when α7 nicotinic acetylcholine receptors expressed in the glutamatergic terminals are expos...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20122943
更新日期：2019-06-16 00:00:00
abstract：:Autoimmunity and hematological malignancies are often concomitant in patients. A causal bidirectional relationship exists between them. Loss of immunological tolerance with inappropriate activation of the immune system, likely due to environmental and genetic factors, can represent a breeding ground for the appearance...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19103139
更新日期：2018-10-12 00:00:00
abstract：:Psychotropics (antipsychotics, mood stabilizers, antidepressants, anxiolytics, etc.) are commonly prescribed to treat Huntington's disease (HD). In HD preclinical models, while no psychotropic has convincingly affected huntingtin gene, HD modifying gene, or huntingtin protein expression, psychotropic neuroprotective e...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms141122558
更新日期：2013-11-15 00:00:00
abstract：:Patients with brain metastasis from renal cell carcinoma (RCC) or melanoma have historically had very poor prognoses of less than one year. Stereotactic radiosurgery (SRS) can be an effective treatment for patients with these tumors. This study analyzes the effect of pretreatment prognostic factors on overall survival...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17030400
更新日期：2016-03-18 00:00:00
abstract：:Sema3C protein, a member of the class 3 family of secreted semaphorins, play an important role in tumor development by regulating cell proliferation, migration, invasion, and angiogenesis processes. Depending on the type and malignancy grade of the tumor, Sema3C function remains controversial. In this study, we constr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20225672
更新日期：2019-11-12 00:00:00
abstract：:Radiation has a wide variety of effects on the liver. Fibrosis is a concern in medical fields as one of the acute effects of high-dose irradiation, such as with cancer radiotherapies. Cancer is also an important concern following exposure to radiation. The liver has an active metabolism and reacts to radiations. In ad...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19123855
更新日期：2018-12-03 00:00:00
abstract：:In biological cells, membrane proteins are the most crucial component for the maintenance of cell physiology and processes, including ion transportation, cell signaling, cell adhesion, and recognition of signal molecules. Therefore, researchers have proposed a number of membrane platforms to mimic the biological cell ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20061437
更新日期：2019-03-21 00:00:00
abstract：:Patients with chronic hepatitis C virus (HCV) develop hepatocellular carcinoma (HCC) regardless of achieving a sustained viral response (SVR). Because advanced liver fibrosis is a powerful risk factor for HCC, we analyzed the association between autotaxin (ATX), a liver fibrosis marker, and post-SVR HCC development wi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124517
更新日期：2020-06-25 00:00:00
abstract：:Redox active selenium (Se) compounds have gained substantial attention in the last decade as potential cancer therapeutic agents. Several Se compounds have shown high selectivity and sensitivity against malignant cells. The cytotoxic effects are exerted by their biologically active metabolites, with methylselenol (CH₃...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19102849
更新日期：2018-09-20 00:00:00
abstract：:Interleukin-18 (IL-18) is a multi-functional immuno-mediator in the development and progression of many types of infectious and inflammatory diseases. In this study, we evaluated the contribution of IL-18 genotypes to renal cell carcinoma (RCC) in Taiwan via the genotyping of IL-18 -656 (A/C), -607 (A/C), and -137 (G/...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20071563
更新日期：2019-03-28 00:00:00
abstract：:Pollination is a crucial stage in plant reproductive process. The self-compatibility (SC) and self-incompatibility (SI) mechanisms determined the plant genetic diversity and species survival. D. chrysanthum is a highly valued ornamental and traditional herbal orchid in Asia but has been declared endangered. The sexual...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18122496
更新日期：2017-11-23 00:00:00
abstract：:Previous studies in our lab revealed that chemical zinc chelation or zinc transporter 3 (ZnT3) gene deletion suppresses the clinical features and neuropathological changes associated with experimental autoimmune encephalomyelitis (EAE). In addition, although protective functions are well documented for AMP-activated p...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21093375
更新日期：2020-05-10 00:00:00
abstract：:During the last few years, diverse studies have shown that tumors can actively interact with the lymphatic system and promote metastases development. In order to examine the molecular mechanisms involved in this interaction, we co-cultured tumor and lymphatic endothelial cells (LEC) and subsequently analyzed the molec...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21020602
更新日期：2020-01-17 00:00:00
abstract：:Gibberellin (GA) is a key signal molecule inducing differentiation of tracheary elements, fibers, and xylogenesis. However the molecular mechanisms underlying the effect of GA on xylem elongation and secondary wall development in tree species remain to be determined. In this study, Betula platyphylla (birch) seeds wer...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160922960
更新日期：2015-09-23 00:00:00
