Abstract：
:Laser-driven accelerators allow to generate ultrashort (from femto- to picoseconds) high peak dose-rate (up to tens of GGy/s) accelerated particle beams. However, the radiobiological effects of ultrashort pulsed irradiation are still poorly studied. The aim of this work was to compare the formation and elimination of γH2AX and 53BP1 foci (well known markers for DNA double-strand breaks (DSBs)) in Hela cells exposed to ultrashort pulsed electron beams generated by Advanced Research Electron Accelerator Laboratory (AREAL) accelerator (electron energy 3.6 MeV, pulse duration 450 fs, pulse repetition rates 2 or 20 Hz) and quasi-continuous radiation generated by Varian accelerator (electron energy 4 MeV) at doses of 250-1000 mGy. Additionally, a study on the dose-response relationships of changes in the number of residual γH2AX foci in HeLa and A549 cells 24 h after irradiation at doses of 500-10,000 mGy were performed. We found no statistically significant differences in γH2AX and 53BP1 foci yields at 1 h after exposure to 2 Hz ultrashort pulse vs. quasi-continuous radiations. In contrast, 20 Hz ultrashort pulse irradiation resulted in 1.27-fold higher foci yields as compared to the quasi-continuous one. After 24 h of pulse irradiation at doses of 500-10,000 mGy the number of residual γH2AX foci in Hela and A549 cells was 1.7-2.9 times higher compared to that of quasi-continuous irradiation. Overall, the obtained results suggest the slower repair rate for DSBs induced by ultrashort pulse irradiation in comparison to DSBs induced by quasi-continuous irradiation.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Babayan N,Vorobyeva N,Grigoryan B,Grekhova A,Pustovalova M,Rodneva S,Fedotov Y,Tsakanova G,Aroutiounian R,Osipov Adoi
10.3390/ijms21249488subject
Has Abstractpub_date
2020-12-14 00:00:00issue
24issn
1422-0067pii
ijms21249488journal_volume
21pub_type
杂志文章abstract：:The purpose of this study is to clinically validate a series of circulating miRNAs that distinguish between the 4 most prevalent tumor types (lung cancer (LC); breast cancer (BC); colorectal cancer (CRC); and prostate cancer (PCa)) and healthy donors (HDs). A total of 18 miRNAs and 3 housekeeping miRNA genes were eval...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21082783
更新日期：2020-04-16 00:00:00
abstract：:Esophageal squamous cell carcinoma (ESCC), a major histologic type of esophageal cancer, is one of the frequent causes of cancer-related death worldwide. Picropodophyllotoxin (PPT) is the main component of Podophyllum hexandrum root with antitumor activity via apoptosis-mediated mechanisms in several cancer cells. How...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21134640
更新日期：2020-06-30 00:00:00
abstract：:DNA alkylating drugs have been used in clinics for more than seventy years. The diversity of their mechanism of action (major/minor groove; mono-/bis-alkylation; intra-/inter-strand crosslinks; DNA stabilization/destabilization, etc.) has undoubtedly major consequences on the cellular response to treatment. The aim of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms161125971
更新日期：2015-11-05 00:00:00
abstract：:Autophagy, a self-defense mechanism, has been found to be associated with drug resistance in hepatocellular carcinoma (HCC). Our study was designed to investigate the role and related mechanisms of autophagy in matrine-induced apoptosis in hepatoma cells of HepG2 and Bel7402. Cell apoptosis was detected by flow cytome...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms141223212
更新日期：2013-11-25 00:00:00
abstract：:The natriuretic peptides (NPs) family includes a class of hormones and their receptors needed for the physiological control of cardiovascular functions. The discovery of NPs provided a fundamental contribution into our understanding of the physiological regulation of blood pressure, and of heart and kidney functions. ...
journal_title：International journal of molecular sciences
pub_type： 社论
doi：10.3390/ijms20163991
更新日期：2019-08-16 00:00:00
abstract：:Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) is known to regulate mitochondrial biogenesis. Resveratrol is present in a variety of plants, including the skin of grapes, blueberries, raspberries, mulberries, and peanuts. It has been shown to offer protective effects against a number of ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20040998
更新日期：2019-02-25 00:00:00
abstract：:Cytokine-induced killer (CIK) cells are heterogeneous, major histocompatibility complex (MHC)-unrestricted T lymphocytes that have acquired the expression of several natural killer (NK) cell surface markers following the addition of interferon gamma (IFN-γ), OKT3 and interleukin-2 (IL-2). Treatment with CIK cells demo...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21093078
更新日期：2020-04-27 00:00:00
abstract：:The tumor microenvironment, which consists of fibroblasts, smooth muscle cells, endothelial cells, immune cells, epithelial cells, and extracellular matrices, plays a crucial role in tumor progression. Hepatic stellate cells (HSCs), a class of unique liver stromal cells, participate in immunomodulatory activities by i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205079
更新日期：2019-10-13 00:00:00
abstract：:The human gastrointestinal tract is inhabited by many types of microbiota, including bacteria, viruses, and fungi. Dysregulations of their microenvironment are associated with various health problems, not only limited to gastrointestinal disorders, such as inflammatory bowel disease, but to impacts beyond the intestin...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20020387
更新日期：2019-01-17 00:00:00
abstract：:Unlike orthodox species, seed recalcitrance is poorly understood, especially at the molecular level. In this regard, seed maturation and germination were studied in the non-orthodox Quercus ilex by using a proteomics strategy based on two-dimensional gel electrophoresis coupled to matrix-assisted laser desorption ioni...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21144870
更新日期：2020-07-09 00:00:00
abstract：:Accurate and sensitive determination of hematopoietic chimerism is a crucial diagnostic measure after allogeneic stem cell transplantation to monitor engraftment and potentially residual disease. Short tandem repeat (STR) amplification, the current "gold standard" for chimerism assessment facilitates reliable accuracy...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17091515
更新日期：2016-09-09 00:00:00
abstract：:Viruses are increasingly recognized as emerging infectious disease agents in both humans and animals.[...]. ...
journal_title：International journal of molecular sciences
pub_type： 社论
doi：10.3390/ijms19020398
更新日期：2018-01-30 00:00:00
abstract：:AMP-activated protein kinase (AMPK) is an important metabolic regulator that mediates cellular adaptation to diverse stresses. One of the AMPK substrates, tuberous sclerosis complex 2 (TSC2), was suggested to mediate AMPK-induced silencing of mTOR complex 1 (mTORC1) signaling that is critical for cell growth. However,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms16047015
更新日期：2015-03-27 00:00:00
abstract：:Tear stability decreases with increasing age and the same signs of instability are exacerbated with dry eye. Meibum lipid compositional changes with age provide insights into the biomolecules responsible for tear film instability. Meibum was collected from 69 normal donors ranging in age from 0.6 to 68 years of age. I...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18091862
更新日期：2017-08-28 00:00:00
abstract：:Hemorrhage is one of the most obvious pathological phenomena in grass carp reovirus (GCRV) infection. The etiology of GCRV-induced hemorrhage is unclear. We found inducible nitric oxide synthase (iNOS) may relate to viral hemorrhage according to the previous studies, which is expressed at high levels after GCRV infect...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20246335
更新日期：2019-12-16 00:00:00
abstract：:Avascular meniscus tears show poor intrinsic regenerative potential. Thus, lesions within this area predispose the patient to developing knee osteoarthritis. Current research focuses on regenerative approaches using growth factors or mesenchymal stem cells (MSCs) to enhance healing capacity within the avascular menisc...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051120
更新日期：2019-03-05 00:00:00
abstract：:Hypertrophic scars and keloids are fibroproliferative disorders that may arise after any deep cutaneous injury caused by trauma, burns, surgery, etc. Hypertrophic scars and keloids are cosmetically problematic, and in combination with functional problems such as contractures and subjective symptoms including pruritus,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,meta分析,评审
doi：10.3390/ijms19030711
更新日期：2018-03-02 00:00:00
abstract：:Sertoli cells are somatic supporting cells in spermatogenic niche and play critical roles in germ cell development, but it is yet to be understood how epigenetic modifiers regulate Sertoli cell development and contribution to spermatogenesis. BRG1 (Brahma related gene 1) is a catalytic subunit of the mammalian SWI/SNF...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124358
更新日期：2020-06-19 00:00:00
abstract：:Future therapeutic intervention that could effectively decelerate the rate of degeneration within the substantia nigra pars compacta (SNc) could add years of mobility and reduce morbidity associated with Parkinson's disease (PD). Neurodegenerative decline associated with PD is distinguished by extensive damage to SNc ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms12010506
更新日期：2011-01-17 00:00:00
abstract：:The N-methyl-(2S,4R)-trans-4-hydroxy-l-proline-enriched fraction (NMP) from Sideroxylon obtusifolium was evaluated as a neuroprotective agent in the intracerebroventricular (icv) pilocarpine (Pilo) model. To this aim, male mice were subdivided into sham (SO, vehicle), Pilo (300 µg/1 µL icv, followed by the vehicle per...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21114188
更新日期：2020-06-11 00:00:00
abstract：:Frizzled receptors (FZDs) are a family of seven-span transmembrane receptors with hallmarks of G protein-coupled receptors (GPCRs) that serve as receptors for secreted Wingless-type (WNT) ligands in the WNT signaling pathway. Functionally, FZDs play crucial roles in regulating cell polarity, embryonic development, cel...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19051543
更新日期：2018-05-22 00:00:00
abstract：:Rho family GTPases belong to the Ras GTPase superfamily and transduce intracellular signals known to regulate a variety of cellular processes, including cell polarity, morphogenesis, migration, apoptosis, vesicle trafficking, viral transport and cellular transformation. The three best-characterized Rho family members ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms14047089
更新日期：2013-03-28 00:00:00
abstract：:The selective binding of six (S)-quinuclidine-triazoles and their (R)-enantiomers to nicotinic acetylcholine receptor (nAChR) subtypes α3β4 and α7, respectively, were analyzed by in silico docking to provide the insight into the molecular basis for the observed stereospecific subtype discrimination. Homology modeling ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21176189
更新日期：2020-08-27 00:00:00
abstract：:Apolipoprotein E (apoE) is mainly secreted by hepatocytes and incorporated into most plasma lipoproteins. Macrophages, which accumulate cholesterol and are critical for the development of the atherosclerotic plaque, are also an important, albeit smaller, apoE source. Distal regulatory elements control cell-specific ac...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20061471
更新日期：2019-03-23 00:00:00
abstract：:The facilitative glucose transporter type 4 (GLUT4) is expressed in adipose and muscle and plays a vital role in whole body glucose homeostasis. In the absence of insulin, only ~1% of cellular GLUT4 is present at the plasma membrane, with the vast majority localizing to intracellular organelles. GLUT4 is retained intr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms14059963
更新日期：2013-05-10 00:00:00
abstract：:The intestinal epithelium constitutes a dynamic physical barrier segregating the luminal content from the underlying mucosal tissue. Following injury, the epithelial integrity is restored by rapid migration of intestinal epithelial cells (IECs) across the denuded area in a process known as wound healing. Hence, throug...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms15069594
更新日期：2014-05-30 00:00:00
abstract：:Glucose variability (GV) has been recognized recently as a promoter of complications and therapeutic targets in diabetes. The aim of this study was to reconstruct and analyze gene networks related to GV in diabetes and its complications. For network analysis, we used the ANDSystem that provides automatic network recon...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228691
更新日期：2020-11-18 00:00:00
abstract：:Benzimidazole derivatives have a diverse range of biological activities, including antiulcer, antihypertensive, antiviral, antifungal, anti-inflammatory, and anticancer. Despite these activities, previous studies have revealed that some of the derivatives can induce mutations. This study aimed to screen for potential ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184324
更新日期：2019-09-04 00:00:00
abstract：:Low temperature stress has a severe impact on the distribution, physiology, and survival of plants in their natural habitats. While numerous studies have focused on the physiological and molecular adjustments to low temperatures, this study provides evidence that cold induced physiological responses coincide with dist...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228753
更新日期：2020-11-19 00:00:00
abstract：:Zinc finger protein 554 (ZNF554), a member of the Krüppel-associated box domain zinc finger protein subfamily, is predominantly expressed in the brain and placenta in humans. Recently, we unveiled that ZNF554 regulates trophoblast invasion during placentation and its decreased expression leads to the early pathogenesi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21165762
更新日期：2020-08-11 00:00:00
