Abstract：
:DNA alkylating drugs have been used in clinics for more than seventy years. The diversity of their mechanism of action (major/minor groove; mono-/bis-alkylation; intra-/inter-strand crosslinks; DNA stabilization/destabilization, etc.) has undoubtedly major consequences on the cellular response to treatment. The aim of this review is to highlight the variety of established protein recognition of DNA adducts to then particularly focus on glyceraldehyde-3-phosphate dehydrogenase (GAPDH) function in DNA adduct interaction with illustration using original experiments performed with S23906-1/DNA adduct. The introduction of this review is a state of the art of protein/DNA adducts recognition, depending on the major or minor groove orientation of the DNA bonding as well as on the molecular consequences in terms of double-stranded DNA maintenance. It reviews the implication of proteins from both DNA repair, transcription, replication and chromatin maintenance in selective DNA adduct recognition. The main section of the manuscript is focusing on the implication of the moonlighting protein GAPDH in DNA adduct recognition with the model of the peculiar DNA minor groove alkylating and destabilizing drug S23906-1. The mechanism of action of S23906-1 alkylating drug and the large variety of GAPDH cellular functions are presented prior to focus on GAPDH direct binding to S23906-1 adducts.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Savreux-Lenglet G,Depauw S,David-Cordonnier MHdoi
10.3390/ijms161125971subject
Has Abstractpub_date
2015-11-05 00:00:00pages
26555-81issue
11issn
1422-0067pii
ijms161125971journal_volume
16pub_type
杂志文章,评审abstract：:Prostate cancer (PCa), an epithelial malignant tumor, is the second common cause of cancer death among males in western countries. Thus, the development of new strategies is urgently needed. Tanshinones isolated from Salvia miltiorrhiza and its synthetic analogs show various biological activities including anticancer ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184459
更新日期：2019-09-10 00:00:00
abstract：:A three-dimensional (3D) representative volume element (RVE) model was developed for analyzing effective mechanical behavior of fiber-reinforced ceramic matrix composites with imperfect interfaces. In the model, the fiber is assumed to be perfectly elastic until its tensile strength, and the ceramic material is modele...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms151223389
更新日期：2014-12-16 00:00:00
abstract：:Point mutations in genes encoding isoforms of skeletal muscle tropomyosin may cause nemaline myopathy, cap myopathy (Cap), congenital fiber-type disproportion (CFTD), and distal arthrogryposis. The molecular mechanisms of muscle dysfunction in these diseases remain unclear. We studied the effect of the E173A, R90P, E1...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19123975
更新日期：2018-12-10 00:00:00
abstract：:We have extracted an extensive collection of recurrent structural motifs (RSMs), which consist of sequentially non-contiguous structural motifs (4-6 residues), each of which appears with very similar conformation in three or more mutually unrelated protein structures. We find that the proteins in our set are covered t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14047795
更新日期：2013-04-10 00:00:00
abstract：:The emergence of multidrug-resistant bacteria is a major global health concern. The search for new therapies has brought bacteriophages into the spotlight, and new phages are being described as possible therapeutic agents. Among the bacteria that are most extensively resistant to current antibiotics is Klebsiella pneu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21093160
更新日期：2020-04-30 00:00:00
abstract：:Thioflavin T (ThT) assay is extensively used for studying fibrillation kinetics in vitro. However, the differences in the time course of ThT fluorescence intensity and lifetime and other physical parameters of the system, such as particle size distribution, raise questions about the correct interpretation of the aggre...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21176169
更新日期：2020-08-26 00:00:00
abstract：:Amaranthus spinosus Linn. (Family: Amaranthaceae) has been shown to be useful in preventing and mitigating adverse pathophysiological conditions and complex diseases. However, only limited information is available on the anticancer potential of this plant. In this study, we examined the antiproliferative and pro-apopt...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17101604
更新日期：2016-09-22 00:00:00
abstract：:The estrogen receptor (ER) signaling regulates numerous physiological processes mainly through activation of gene transcription (genomic pathways). Caveolin1 (CAV1) is a membrane-resident protein that behaves as platform to enable different signaling molecules and receptors for membrane-initiated pathways. CAV1 direct...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21175989
更新日期：2020-08-20 00:00:00
abstract：:Chlorpyrifos (CPF) is widely used in the prevention and control of crop pests and diseases in agriculture. However, the irrational utilization of pesticides not only causes environmental pollution but also threatens human health. Compared with the conventional techniques for the determination of pesticides in soil, su...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20112817
更新日期：2019-06-10 00:00:00
abstract：:Non-bullous congenital ichthyosiform erythroderma (NBCIE) is a hereditary disorder of keratinization caused by pathogenic variants in genes encoding enzymes important to lipid processing and terminal keratinocyte differentiation. Impaired function of these enzymes can cause pathologic epidermal scaling, significantly ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160921791
更新日期：2015-09-09 00:00:00
abstract：:The genetic diversity and population genetic structure of 252 accessions from 21 Prunus sibirica L. populations were investigated using 10 ISSR, SSR, and SRAP markers. The results suggest that the entire population has a relatively high level of genetic diversity, with populations HR and MY showing very high diversity...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15010377
更新日期：2013-12-31 00:00:00
abstract：:Ginsenoside Rd (GSRd), one of the main active monomer compounds from the medical plant Panax ginseng, has been shown to promote neuronal survival in models of ischemic cerebral damage. As an extending study, here we examined whether GSRd could exert a beneficial effect in an experimental Parkinson disease (PD) model i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160714395
更新日期：2015-06-24 00:00:00
abstract：:The tumor vasculature is essential for tumor growth and metastasis, and is a prime target of several anti-cancer agents. Increasing evidence indicates that tumor angiogenesis is stimulated by extracellular vesicles (EVs) that are secreted or shed by cancer cells. These EVs encapsulate a variety of biomolecules with an...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21155418
更新日期：2020-07-30 00:00:00
abstract：:We built new hybrid devices consisting of chemical vapor deposition (CVD) grown carbon nanotube (CNT) transistors, decorated with TbPc(2) (Pc = phthalocyanine) rare-earth based single-molecule magnets (SMMs). The drafting was achieved by tailoring supramolecular π-π interactions between CNTs and SMMs. The magnetoresis...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12106656
更新日期：2011-01-01 00:00:00
abstract：:Four-stranded DNA structures were structurally characterized in vitro by NMR, X-ray and Circular Dichroism spectroscopy in detail. Among the different types of quadruplexes (i-Motifs, minor groove quadruplexes, G-quadruplexes, etc.), the best described are G-quadruplexes which are featured by Hoogsteen base-paring. Se...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms151017493
更新日期：2014-09-29 00:00:00
abstract：:In the absence of visible mucosal damage, it is hypothesized that the esophageal mucosal barrier is functionally impaired in patients with non-erosive reflux disease (NERD). The aim of the present study was to perform an exploratory analysis of the mucosal barrier in NERD compared to erosive esophagitis (EE) and contr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18051091
更新日期：2017-05-19 00:00:00
abstract：:Erigeron breviscapus (Vant.) Hand.-Mazz. is a famous traditional Chinese medicine that has positive effects on the treatment of cardiovascular and cerebrovascular diseases. With the increase of market demand (RMB 500 million per year) and the sharp decrease of wild resources, it is an urgent task to cultivate high-qua...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041458
更新日期：2020-02-20 00:00:00
abstract：:This article presents an experimental analysis of the organic content of two serpentinized peridotite rocks of the terrestrial upper mantle. The samples have been dredged on the floor of the Ashadze and Logatchev hydrothermal sites on the Mid-Atlantic Ridge. In this preliminary analysis, amino acids and long chain n-a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms10072986
更新日期：2009-07-03 00:00:00
abstract：:Antioxidant capacity (AOC) against peroxyl radical and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical cation was measured for a series of p-hydroxybenzoic (HB) and p-hydroxycinnamic (HC) acids at different pH. Quantum-chemical computation was performed using Gaussian 3.0 softwa...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150916351
更新日期：2014-09-16 00:00:00
abstract：:Sarcopenia, the degenerative and systemic loss of skeletal muscle mass, indicates patient frailty and impaired physical function. Sarcopenia can be caused by multiple factors, including advanced age, lack of exercise, poor nutritional status, inflammatory diseases, endocrine diseases, and malignancies. In patients wit...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19102999
更新日期：2018-10-01 00:00:00
abstract：:The Prostate Health Index (PHI) has been used increasingly in the context of prostate cancer (PCa) diagnostics since 2010. Previous studies have shown an association between PHI and a tumor volume of >0.5 cm³. The aim of this study was to investigate the correlation between PHI and tumor volume as well as the Gleason ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18030488
更新日期：2017-02-24 00:00:00
abstract：:Pseudomonas aeruginosa and Staphylococcus aureus are two major pathogens involved in a large variety of infections. Their co-occurrence in the same site of infection has been frequently reported and is linked to enhanced virulence and difficulty of treatment. Herein, the antimicrobial and antibiofilm activities of an ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20143604
更新日期：2019-07-23 00:00:00
abstract：:While drug resistant mutations in HIV-1 are largely credited to its error prone HIV-1 RT, the time point in the infection cycle that these mutations can arise and if they appear spontaneously without selection pressures both remained enigmatic. Many HIV-1 RT mutational in vitro studies utilized reporter genes (LacZ) a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22010370
更新日期：2020-12-31 00:00:00
abstract：:The use of mesenchymal stem cells (MSC) for the treatment of cutaneous wounds is currently of enormous interest. However, the broad translation of cell therapies into clinical use is hampered by their efficacy, safety, manufacturing and cost. MSCs release a broad repertoire of trophic factors and immunomodulatory cyto...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21197038
更新日期：2020-09-24 00:00:00
abstract：:The diversity and secondary metabolite potential of culturable actinomycetes associated with eight different marine sponges collected from the South China Sea and the Yellow sea were investigated. A total of 327 strains were isolated and 108 representative isolates were selected for phylogenetic analysis. Ten families...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13055917
更新日期：2012-01-01 00:00:00
abstract：:Platelets and platelet microparticles (PMPs) play a key role in the pathophysiology of vascular disorders such as coronary artery disease and stroke. In atherosclerosis, for example, the disruption of the plaque exposes endogenous agonists such as collagen, which activates platelets. Platelet hyper-activation and the ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21010146
更新日期：2019-12-24 00:00:00
abstract：:Argininosuccinate synthetase (ASS), a key enzyme to synthesize arginine is down regulated in many tumors including hepatocellular carcinoma (HCC). Similar to previous reports, we have found the decrease in ASS expression in poorly differentiated HCC. These ASS(-) tumors are auxotrophic for arginine. Pegylated arginine...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18061175
更新日期：2017-06-01 00:00:00
abstract：:Wound healing is a complex process with a linear development that involves many actors in a multistep timeline commonly divided into four stages: Hemostasis, inflammation, proliferation, and remodeling. Chronic non-healing wounds fail to progress beyond the inflammatory phase, thus precluding the next steps and, ultim...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20153721
更新日期：2019-07-30 00:00:00
abstract：:Interleukin (IL)-4 and -13 are structurally and functionally related cytokines sharing common receptor subunits. They regulate immune responses and, moreover, are involved in the pathogenesis of a variety of human neoplasms. Three different receptors have been described for IL-4, but only IL-4 receptor type II (IL-4Rα...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms22020727
更新日期：2021-01-13 00:00:00
abstract：:It is well known that cobalt chloride (CoCl2) can enhance the stability of hypoxia-inducible factor (HIF)-1α. The aim of this study is to detect the effect of CoCl2 on the hypoxia tolerance of mice which were repeatedly exposed to autoprogressive hypoxia. Balb/c mice were randomly divided into groups of chemical pretr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150610999
更新日期：2014-06-18 00:00:00
