当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES期刊下所有文献 > Digital PCR Panel for Sensitive Hematopoietic Chimerism Quantification after Allogeneic Stem Cell Transplantation.

Digital PCR Panel for Sensitive Hematopoietic Chimerism Quantification after Allogeneic Stem Cell Transplantation.

Abstract：

:Accurate and sensitive determination of hematopoietic chimerism is a crucial diagnostic measure after allogeneic stem cell transplantation to monitor engraftment and potentially residual disease. Short tandem repeat (STR) amplification, the current "gold standard" for chimerism assessment facilitates reliable accuracy, but is hampered by its limited sensitivity (≥1%). Digital PCR (dPCR) has been shown to combine exact quantification and high reproducibility over a very wide measurement range with excellent sensitivity (routinely ≤0.1%) and thus represents a promising alternative to STR analysis. We here aimed at developing a whole panel of digital-PCR based assays for routine diagnostic. To this end, we tested suitability of 52 deletion/insertion polymorphisms (DIPs) for duplex analysis in combination with either a reference gene or a Y-chromosome specific PCR. Twenty-nine DIPs with high power of discrimination and good performance were identified, optimized and technically validated. We tested the newly established assays on retrospective patient samples that were in parallel also measured by STR amplification and found excellent correlation. Finally, a screening plate for initial genotyping with DIP-specific duplex dPCR assays was designed for convenient assay selection. In conclusion, we have established a comprehensive dPCR system for precise and high-sensitivity measurement of hematopoietic chimerism, which should be highly useful for clinical routine diagnostics.

journal_name

Int J Mol Sci

journal_title

International journal of molecular sciences

authors

Stahl T,Rothe C,Böhme MU,Kohl A,Kröger N,Fehse B

doi

10.3390/ijms17091515

subject

Has Abstract

pub_date

2016-09-09 00:00:00

issue

9

issn

1422-0067

pii

ijms17091515

journal_volume

17

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES文献大全
  • Develop a High-Throughput Screening Method to Identify C-P4H1 (Collagen Prolyl 4-Hydroxylase 1) Inhibitors from FDA-Approved Chemicals.

    abstract：:Collagen prolyl 4-hydroxylase 1 (C-P4H1) is an α-ketoglutarate (α-KG)-dependent dioxygenase that catalyzes 4-hydroxylation of proline on collagen. C-P4H1-induced prolyl hydroxylation is required for proper collagen deposition and cancer metastasis. Therefore, targeting C-P4H1 is considered a potential therapeutic stra...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21186613

    authors： Wang S,Lee KH,Araujo NV,Zhan CG,Rangnekar VM,Xu R

    更新日期：2020-09-10 00:00:00

  • Cell Death Conversion under Hypoxic Condition in Tumor Development and Therapy.

    abstract：:Hypoxia, which is common during tumor progression, plays important roles in tumor biology. Failure in cell death in response to hypoxia contributes to progression and metastasis of tumors. On the one hand, the metabolic and oxidative stress following hypoxia could lead to cell death by triggering signal cascades, like...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms161025536

    authors： Qiu Y,Li P,Ji C

    更新日期：2015-10-23 00:00:00

  • LARP6 Meets Collagen mRNA: Specific Regulation of Type I Collagen Expression.

    abstract：:Type I collagen is the most abundant structural protein in all vertebrates, but its constitutive rate of synthesis is low due to long half-life of the protein (60-70 days). However, several hundred fold increased production of type I collagen is often seen in reparative or reactive fibrosis. The mechanism which is res...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms17030419

    authors： Zhang Y,Stefanovic B

    更新日期：2016-03-22 00:00:00

  • Suppression of Pax3-MITF-M Axis Protects from UVB-Induced Skin Pigmentation by Tetrahydroquinoline Carboxamide.

    abstract：:Paired box gene 3 (Pax3) and cAMP responsive element-binding protein (CREB) directly interact with the cis-acting elements on the promoter of microphthalmia-associated transcription factor isoform M (MITF-M) for transcriptional activation in the melanogenic process. Tyrosinase (Tyro) is a target gene of MITF-M, and fu...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21249631

    authors： Choi YP,Kim GH,Kim SH,Maeng J,Lee H,Han SB,Kim KH,Kim Y

    更新日期：2020-12-17 00:00:00

  • Nrf2, the Master Regulator of Anti-Oxidative Responses.

    abstract：:Tight regulation of inflammation is very important to guarantee a balanced immune response without developing chronic inflammation. One of the major mediators of the resolution of inflammation is the transcription factor: the nuclear factor erythroid 2-like 2 (Nrf2). Stabilized following oxidative stress, Nrf2 induces...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms18122772

    authors： Vomund S,Schäfer A,Parnham MJ,Brüne B,von Knethen A

    更新日期：2017-12-20 00:00:00

  • Role of Hypoxia and Metabolism in the Development of Neointimal Hyperplasia in Arteriovenous Fistulas.

    abstract：:For patients with end-stage renal disease requiring hemodialysis, their vascular access is both their lifeline and their Achilles heel. Despite being recommended as primary vascular access, the arteriovenous fistula (AVF) shows sub-optimal results, with about 50% of patients needing a revision during the year followin...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20215387

    authors： Sadaghianloo N,Contenti J,Dardik A,Mazure NM

    更新日期：2019-10-29 00:00:00

  • Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins.

    abstract：:Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration. Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols. In this study, ALP-loaded gellan gum (GG) h...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21072315

    authors： Douglas TEL,Keppler JK,Vandrovcová M,Plencner M,Beranová J,Feuereisen M,Parakhonskiy BV,Svenskaya Y,Atkin V,Ivanova A,Ricquier P,Balcaen L,Vanhaecke F,Schieber A,Bačáková L,Skirtach AG

    更新日期：2020-03-27 00:00:00

  • Downregulation of FOXP1 Inhibits Cell Proliferation in Hepatocellular Carcinoma by Inducing G1/S Phase Cell Cycle Arrest.

    abstract：:Forkhead box P1 (FOXP1) belongs to a family of winged-helix transcription factors that are involved in the processes of cellular proliferation, differentiation, metabolism, and longevity. FOXP1 can affect cell proliferation and migratory ability in hepatocellular carcinoma (HCC) in vitro. However, little is known abou...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms17091501

    authors： Wang X,Sun J,Cui M,Zhao F,Ge C,Chen T,Yao M,Li J

    更新日期：2016-09-08 00:00:00

  • Optimization and evaluation of magnetic bead separation combined with matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS) for proteins profiling of peritoneal dialysis effluent.

    abstract：:Peritoneal dialysis effluent (PDE) potentially carries an archive of peptides relevant to pathological processes in abdominal and surrounding tissues. Magnetic beads and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is one such approach that offers a unique tool for profiling of peptides...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms15011162

    authors： Guo N,Wen Q,Li ZJ,Xu RC,Peng FF,Yu XQ

    更新日期：2014-01-16 00:00:00

  • Microbiota-Associated Therapy for Non-Alcoholic Steatohepatitis-Induced Liver Cancer: A Review.

    abstract：:Even though advancement in medicine has contributed to the control of many diseases to date, cancer therapy continues to pose several challenges. Hepatocellular carcinoma (HCC) etiology is multifactorial. Recently, non-alcoholic fatty liver disease (NAFLD) has been considered as an important risk factor of HCC. NAFLD ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21175999

    authors： Chen YH,Wu WK,Wu MS

    更新日期：2020-08-20 00:00:00

  • Designer Biopolymers: Self-Assembling Proteins and Nucleic Acids.

    abstract：:Nature has evolved sequence-controlled polymers such as DNA and proteins over its long history [...]. ...

    journal_title：International journal of molecular sciences

    pub_type： 社论

    doi：10.3390/ijms21093276

    authors： Sugawara-Narutaki A,Kamiya Y

    更新日期：2020-05-06 00:00:00

  • Biliverdin Reductase A (BVRA) Mediates Macrophage Expression of Interleukin-10 in Injured Kidney.

    abstract：:Biliverdin reductase A is an enzyme, with serine/threonine/tyrosine kinase activation, converting biliverdin (BV) to bilirubin (BR) in heme degradation pathway. It has been reported to have anti-inflammatory and antioxidant effect in monocytes and human glioblastoma. However, the function of BVRA in polarized macropha...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms160922621

    authors： Hu Z,Pei G,Wang P,Yang J,Zhu F,Guo Y,Wang M,Yao Y,Zeng R,Liao W,Xu G

    更新日期：2015-09-18 00:00:00

  • Structural Biology of STAT3 and Its Implications for Anticancer Therapies Development.

    abstract：:Transcription factors are proteins able to bind DNA and induce the transcription of specific genes. Consequently, they play a pivotal role in multiple cellular pathways and are frequently over-expressed or dysregulated in cancer. Here, we will focus on a specific "signal transducer and activator of transcription" (STA...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms19061591

    authors： Sgrignani J,Garofalo M,Matkovic M,Merulla J,Catapano CV,Cavalli A

    更新日期：2018-05-28 00:00:00

  • Zinc Homeostasis in Platelet-Related Diseases.

    abstract：:Zn2+ deficiency in the human population is frequent in underdeveloped countries. Worldwide, approximatively 2 billion people consume Zn2+-deficient diets, accounting for 1-4% of deaths each year, mainly in infants with a compromised immune system. Depending on the severity of Zn2+ deficiency, clinical symptoms are ass...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20215258

    authors： Mammadova-Bach E,Braun A

    更新日期：2019-10-23 00:00:00

  • Jasmonic Acid Methyl Ester Induces Xylogenesis and Modulates Auxin-Induced Xylary Cell Identity with NO Involvement.

    abstract：:In Arabidopsis basal hypocotyls of dark-grown seedlings, xylary cells may form from the pericycle as an alternative to adventitious roots. Several hormones may induce xylogenesis, as Jasmonic acid (JA), as well as indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) auxins, which also affect xylary identity. Stu...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20184469

    authors： Della Rovere F,Fattorini L,Ronzan M,Falasca G,Altamura MM,Betti C

    更新日期：2019-09-10 00:00:00

  • Supplemental 25-hydroxycholecalciferol Alleviates Inflammation and Cardiac Fibrosis in Hens.

    abstract：:Broiler breeder hens with efficient feed conversion rate under restricted feed intake (R-hens) or allowed unlimited access to feed (Ad-hens) progressed with cardiac functional failure and suffered early sudden death. A supplement of 69 μg 25-hydroxycholecalciferol (25-OH-D3)/kg feed improved heart health and rescued l...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21218379

    authors： Chou PC,Chen YH,Chung TK,Walzem RL,Lai LS,Chen SE

    更新日期：2020-11-08 00:00:00

  • New Insights into Mechanisms and Functions of Chemokine (C-X-C Motif) Receptor 4 Heteromerization in Vascular Smooth Muscle.

    abstract：:Recent evidence suggests that C-X-C chemokine receptor type 4 (CXCR4) heteromerizes with α1A/B-adrenoceptors (AR) and atypical chemokine receptor 3 (ACKR3) and that CXCR4:α1A/B-AR heteromers are important for α₁-AR function in vascular smooth muscle cells (VSMC). Structural determinants for CXCR4 heteromerization and ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms17060971

    authors： Evans AE,Tripathi A,LaPorte HM,Brueggemann LI,Singh AK,Albee LJ,Byron KL,Tarasova NI,Volkman BF,Cho TY,Gaponenko V,Majetschak M

    更新日期：2016-06-20 00:00:00

  • The melatonergic system in mood and anxiety disorders and the role of agomelatine: implications for clinical practice.

    abstract：:Melatonin exerts its actions through membrane MT1/MT2 melatonin receptors, which belong to the super family of G-protein-coupled receptors consisting of the typical seven transmembrane domains. MT1 and MT2 receptors are expressed in various tissues of the body either as single ones or together. A growing literature su...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms140612458

    authors： De Berardis D,Marini S,Fornaro M,Srinivasan V,Iasevoli F,Tomasetti C,Valchera A,Perna G,Quera-Salva MA,Martinotti G,di Giannantonio M

    更新日期：2013-06-13 00:00:00

  • Phlorotannins from Fucus vesiculosus: Modulation of Inflammatory Response by Blocking NF-κB Signaling Pathway.

    abstract：:Due to their large spectrum of bioactive properties, much attention has recently been drawn to phlorotannins-i.e., phenolic compounds characteristic from brown macroalgae. This study aimed to evaluate the antioxidant and anti-inflammatory properties of F. vesiculosus phlorotannin extracts and purified fractions. Overa...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21186897

    authors： Catarino MD,Silva A,Cruz MT,Mateus N,Silva AMS,Cardoso SM

    更新日期：2020-09-20 00:00:00

  • Salidroside Ameliorates Renal Interstitial Fibrosis by Inhibiting the TLR4/NF-κB and MAPK Signaling Pathways.

    abstract：:Salidroside (Sal) is an active ingredient that is isolated from Rhodiola rosea, which has been reported to have anti-inflammatory activities and a renal protective effect. However, the role of Sal on renal fibrosis has not yet been elucidated. Here, the purpose of the current study is to test the protective effects of...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20051103

    authors： Li R,Guo Y,Zhang Y,Zhang X,Zhu L,Yan T

    更新日期：2019-03-04 00:00:00

  • Human NANOS1 Represses Apoptosis by Downregulating Pro-Apoptotic Genes in the Male Germ Cell Line.

    abstract：:While two mouse NANOS paralogues, NANOS2 and NANOS3, are crucial for maintenance of germ cells by suppression of apoptosis, the mouse NANOS1 paralogue does not seem to regulate these processes. Previously, we described a human NANOS1 p.[(Pro34Thr);(Ser83del)] mutation associated with the absence of germ cells in semin...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21083009

    authors： Janecki DM,Ilaslan E,Smialek MJ,Sajek MP,Kotecki M,Ginter-Matuszewska B,Krainski P,Jaruzelska J,Kusz-Zamelczyk K

    更新日期：2020-04-24 00:00:00

  • Resveratrol Promotes Mitochondrial Biogenesis and Protects against Seizure-Induced Neuronal Cell Damage in the Hippocampus Following Status Epilepticus by Activation of the PGC-1α Signaling Pathway.

    abstract：:Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) is known to regulate mitochondrial biogenesis. Resveratrol is present in a variety of plants, including the skin of grapes, blueberries, raspberries, mulberries, and peanuts. It has been shown to offer protective effects against a number of ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20040998

    authors： Chuang YC,Chen SD,Hsu CY,Chen SF,Chen NC,Jou SB

    更新日期：2019-02-25 00:00:00

  • The Impact of O-Glycosylation on Cyanidin Interaction with RBCs and HMEC-1 Cells-Structure⁻Activity Relationships.

    abstract：:With the aim of contributing to the knowledge about their potential therapeutic activity, we determined the biological activities of cyanidin and its selected O-glycosides in relation to erythrocytes (RBCs) and human dermal vascular endothelial cells (HMEC-1). Furthermore, on the basis of changes in the physical/funct...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20081928

    authors： Cyboran-Mikołajczyk S,Solarska-Ściuk K,Mieszała K,Glatzel-Plucińska N,Matczak K,Kleszczyńska H

    更新日期：2019-04-19 00:00:00

  • A Novel Mutation of the NARROW LEAF 1 Gene Adversely Affects Plant Architecture in Rice (Oryza sativa L.).

    abstract：:Plant architecture is critical for enhancing the adaptability and productivity of crop plants. Mutants with an altered plant architecture allow researchers to elucidate the genetic network and the underlying mechanisms. In this study, we characterized a novel nal1 rice mutant with short height, small panicle, and narr...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21218106

    authors： Subudhi PK,Garcia RS,Coronejo S,De Leon TB

    更新日期：2020-10-30 00:00:00

  • The Metabolic Fate of ortho-Quinones Derived from Catecholamine Metabolites.

    abstract：:ortho-Quinones are produced in vivo through the oxidation of catecholic substrates by enzymes such as tyrosinase or by transition metal ions. Neuromelanin, a dark pigment present in the substantia nigra and locus coeruleus of the brain, is produced from dopamine (DA) and norepinephrine (NE) via an interaction with cys...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms17020164

    authors： Ito S,Yamanaka Y,Ojika M,Wakamatsu K

    更新日期：2016-01-27 00:00:00

  • Targeting IgG in Arthritis: Disease Pathways and Therapeutic Avenues.

    abstract：:Rheumatoid arthritis (RA) is a polygenic and multifactorial syndrome. Many complex immunological and genetic interactions are involved in the final outcome of the clinical disease. Autoantibodies (rheumatoid factors, anti-citrullinated peptide/protein antibodies) are present in RA patients' sera for a long time before...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms19030677

    authors： Nandakumar KS

    更新日期：2018-02-28 00:00:00

  • Antifungal activity of (KW)n or (RW)n peptide against Fusarium solani and Fusarium oxysporum.

    abstract：:The presence of lysine (Lys) or arginine (Arg) and tryptophan (Trp) are important for the antimicrobial effects of cationic peptides. Therefore, we designed and synthesized a series of antimicrobial peptides with various numbers of Lys (or Arg) and Trp repeats [(KW and RW)(n)-NH(2), where n equals 2, 3, 4, or 5]. Anti...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms131115042

    authors： Gopal R,Na H,Seo CH,Park Y

    更新日期：2012-11-15 00:00:00

  • Discovery of Akt kinase inhibitors through structure-based virtual screening and their evaluation as potential anticancer agents.

    abstract：:Akt acts as a pivotal regulator in the PI3K/Akt signaling pathway and represents a potential drug target for cancer therapy. To search for new inhibitors of Akt kinase, we performed a structure-based virtual screening using the DOCK 4.0 program and the X-ray crystal structure of human Akt kinase. From the virtual scre...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms16023202

    authors： Chuang CH,Cheng TC,Leu YL,Chuang KH,Tzou SC,Chen CS

    更新日期：2015-02-02 00:00:00

  • The Relationships Between Stress, Mental Disorders, and Epigenetic Regulation of BDNF.

    abstract：:Brain-derived neurotrophic factor (BDNF), a critical member of the neurotrophic family, plays an important role in multiple stress-related mental disorders. Although alterations in BDNF in multiple brain regions of individuals experiencing stress have been demonstrated in previous studies, it appears that a set of ele...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21041375

    authors： Miao Z,Wang Y,Sun Z

    更新日期：2020-02-18 00:00:00

  • Peptidyl Arginine Deiminase 2 (PADI2)-Mediated Arginine Citrullination Modulates Transcription in Cancer.

    abstract：:Protein arginine deimination leading to the non-coded amino acid citrulline remains a key question in the field of post-translational modifications ever since its discovery by Rogers and Simmonds in 1958. Citrullination is catalyzed by a family of enzymes called peptidyl arginine deiminases (PADIs). Initially, increas...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21041351

    authors： Beato M,Sharma P

    更新日期：2020-02-17 00:00:00