Abstract：
:Peritoneal dialysis effluent (PDE) potentially carries an archive of peptides relevant to pathological processes in abdominal and surrounding tissues. Magnetic beads and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is one such approach that offers a unique tool for profiling of peptides, but this approach has not been used in the PDE analysis. In this study, we developed a strategy for screening PDE proteins <15 kDa and applied this technique to identify potential biomarkers for peritonitis. We examined four kinds of magnetic beads, including a carbon series (C3, C8), weak cation exchange (WCX) and immobilized metal-affinity chromatography (IMAC-Cu) beads. Samples processed with IMAC-Cu magnetic beads consistently showed more MS signals across all beads within the measured mass range. Moreover, there was no difference in the number and morphology of MS signals between concentrated and unconcentrated samples. The PDE peptidome pattern, based on a panel of 15 peaks, accurately recognized peritonitis PD patients from peritonitis-free patients with sensitivity of 90.5% and specificity of 94.7% respectively. Therefore, IMAC-Cu magnetic beads and unconcentrated samples can be used as a fast and cost-effective approach for sample preparation prior to more in-depth discovery of predictive biomarkers of disease in patients on dialysis.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Guo N,Wen Q,Li ZJ,Xu RC,Peng FF,Yu XQdoi
10.3390/ijms15011162subject
Has Abstractpub_date
2014-01-16 00:00:00pages
1162-75issue
1issn
1422-0067pii
ijms15011162journal_volume
15pub_type
杂志文章abstract：:Roots are vital to plant survival and crop yield, yet few efforts have been made to characterize the expressed genes in the roots of non-model plants (root transcriptomes). This study was conducted to sequence, assemble, annotate, and characterize the root transcriptomes of three caladium cultivars (Caladium × hortula...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18040712
更新日期：2017-03-27 00:00:00
abstract：:Renal proximal tubular epithelial cells are significantly damaged during acute kidney injury. Renal proximal tubular cell-specific autophagy-deficient mice show increased sensitivity against renal injury, while showing few pathological defects under normal fed conditions. Considering that autophagy protects the proxim...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010155
更新日期：2019-12-25 00:00:00
abstract：:A previous report showed that both Pyruvatedecarboxylase (PDC) genes were significantly upregulated in kiwifruit after waterlogging treatment using Illumina sequencing technology, and that the kiwifruit AdPDC1 gene was required during waterlogging, but might not be required during other environmental stresses. Here, t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18112377
更新日期：2017-11-09 00:00:00
abstract：:Chronic inflammation in adipose tissue may contribute to depot-specific adipose tissue expansion, leading to obesity and insulin resistance. Dietary supplementation with quercetin or botanical extracts containing quercetin attenuates high fat diet (HFD)-induced obesity and insulin resistance and decreases inflammation...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19030895
更新日期：2018-03-17 00:00:00
abstract：:Ovarian cancer is the seventh most commonly diagnosed cancer amongst women and has the highest mortality rate of all gynaecological malignancies. It is a heterogeneous disease attributed to one of three cell types found within the reproductive milieu: epithelial, stromal, and germ cell. Each histotype differs in etiol...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20061466
更新日期：2019-03-22 00:00:00
abstract：:Sudden sensorineural hearing loss (SSNHL) is a medical emergency, making detailed examination to determine possible causes and early treatment important. However, etiological examinations in SSNHL do not always reveal a cause, and several factors have been found to affect treatment outcomes. Various studies are being ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21197248
更新日期：2020-09-30 00:00:00
abstract：:The particle sizes of pharmaceutical substances are important for their bioavailability. Bioavailability can be improved by reducing the particle size of the drug. In this study, artemisinin was micronized by the rapid expansion of supercritical solutions (RESS). The particle size of the unprocessed white needle-like ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13045060
更新日期：2012-01-01 00:00:00
abstract：:In higher organisms, epithelia separate compartments in order to guarantee their proper function. Such structures are able to seal but also to allow substances to pass. Within the paracellular pathway, a supramolecular structure, the tight junction transport is largely controlled by the temporospatial regulation of it...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20215504
更新日期：2019-11-05 00:00:00
abstract：:Neuroblastoma is a common childhood cancer with almost a third of those affected still dying, thus new therapeutic strategies need to be explored. Current experimental therapies focus mostly on inhibiting oncogenic transcription factor signalling. Although LIN28B, DICER and other RNA-binding proteins (RBPs) have repor...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21145098
更新日期：2020-07-19 00:00:00
abstract：:The antifungal activity of polygodial, a secondary metabolite extracted from Canelo, on mycelial growth of different Botrytis cinerea isolates has been evaluated. The results show that polygodial affects growth of normal and resistant isolates of B. cinerea with EC50 values ranging between 117 and 175 ppm. In addition...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18112251
更新日期：2017-10-27 00:00:00
abstract：BACKGROUND:The determinants that mediate the interactions between microRNAs and the gut microbiome impacting on obesity are scarcely understood. Thus, the aim of this study was to investigate possible interactions between circulating microRNAs and gut microbiota composition in obesity. METHOD:The sample comprised 78 s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249509
更新日期：2020-12-14 00:00:00
abstract：:Type 2 diabetes mellitus (T2D) is a chronic metabolic disease resulting from insulin resistance and progressively reduced insulin secretion, which leads to impaired glucose utilization, dyslipidemia and hyperinsulinemia and progressive pancreatic beta cell dysfunction. The incidence of type 2 diabetes mellitus is incr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21186842
更新日期：2020-09-18 00:00:00
abstract：:Octamer-binding transcription factor 4 (Oct4) is a member of POU (Pit-Oct-Unc) transcription factor family Class V that plays a crucial role in maintaining the pluripotency and self-renewal of stem cells. Though it has been deeply investigated in mammals, its lower vertebrate homologue, especially in the marine fish, ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18010231
更新日期：2017-01-23 00:00:00
abstract：:The interaction between the pituitary hormone, adrenocorticotropin (ACTH), and melanocortin-2 receptor (MC2R) orthologs involves the H6 F7 R8 W9 and R/K15 K16 R17 R18 motifs in ACTH making contact with corresponding contact sites on MC2R. Earlier studies have localized the common HFRW binding site of all melanocortin ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20174166
更新日期：2019-08-26 00:00:00
abstract：:Autophagy is an important process by which pathogens and damaged or unused organelles are eliminated. The role of autophagy in development and the immune response to pathogens is well established. Autophagy-related protein 8 (Atg8) is involved in the formation of the autophagosome and, with the help of the serine prot...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21082944
更新日期：2020-04-22 00:00:00
abstract：:Metastatic spread represents the leading cause of disease-related mortality among cancer patients. Many cancer patients suffer from metastatic relapse years or even decades after radical surgery for the primary tumor. This clinical phenomenon is explained by the early dissemination of cancer cells followed by a long p...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21197304
更新日期：2020-10-03 00:00:00
abstract：:The success of antibody therapeutics is strongly influenced by their multifunctional nature that couples antigen recognition mediated by their variable regions with effector functions and half-life extension mediated by a subset of their constant regions. Nevertheless, the monospecific IgG format is not optimal for ma...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21207496
更新日期：2020-10-12 00:00:00
abstract：:The interactions between chiral molecules and cell membranes have attracted more and more attention in recent decades, due to their importance in molecular science and medical applications. It is observed that some peptides composed of different chiral amino acids may have distinct interactions with a membrane. How do...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20194760
更新日期：2019-09-25 00:00:00
abstract：:Osteosarcoma is the most common primary malignant bone tumour in children and adolescents. Due to micrometastatic spread, radical surgery alone rarely results in cure. Introduction of combination chemotherapy in the 1970s, however, dramatically increased overall survival rates from 20% to approximately 70%. Unfortunat...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21186885
更新日期：2020-09-19 00:00:00
abstract：:Apolipoprotein M (apoM) is a plasma apolipoprotein that mainly associates with high-density lipoproteins. Hence, most studies on apoM so far have investigated its effect on and association with lipid metabolism and atherosclerosis. The insight into apoM biology recently took a major turn. ApoM was identified as a carr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14034419
更新日期：2013-02-25 00:00:00
abstract：:MTH1 (MutT homolog 1) or NUDT1 (Nudix Hydrolase 1), also known as oxidized purine nucleoside triphosphatase, has potential as a biomarker for monitoring cancer progression and quantifying target engagement for relevant therapies. In this study, we validate one MTH1 inhibitor TH287 as a PET MTH1 radiotracer. TH287 was ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228860
更新日期：2020-11-23 00:00:00
abstract：:One of the hallmarks of the acute toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a drastically reduced feed intake by an unknown mechanism. To further elucidate this wasting syndrome, we followed the effects of a single large dose (100 μg/kg) of TCDD on the serum levels of several energy balance-influencing...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150813938
更新日期：2014-08-12 00:00:00
abstract：:In this paper, we show a strategy to modify salphen-type Schiff base ligands with naphtol (SYML1) and pyrocathecol (2,3-dihydroxyphenyl) groups (SYML2), or a combination of both (ASYML). Each of these ligands can be used to obtain polynuclear metal complexes following two different strategies. One relies on using meta...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21103574
更新日期：2020-05-18 00:00:00
abstract：:The progression of cancer is associated with increases in amino acid uptake by cancer cells. Upon their entry into cells through specific transporters, exogenous amino acids are used to synthesize proteins, nucleic acids and lipids and to generate ATP. The essential amino acid leucine is also important for maintaining...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19082373
更新日期：2018-08-12 00:00:00
abstract：:The objective of the present study is to determine whether methoxychlor (MXC) exposure in adulthood affects rat Leydig cell regeneration and to compare its effects with estradiol (E2). Adult 90-day-old male Sprague-Dawley rats received ethane dimethane sulfonate (EDS) to eliminate the adult Leydig cell population. Sub...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15057812
更新日期：2014-05-06 00:00:00
abstract：:Current outcome prediction markers for localized prostate cancer (PCa) are insufficient. The impact of the lipid-modifying Sphingomyelin Phosphodiesterase Acid Like 3B (SMPDL3B) in PCa is unknown. Two cohorts of patients with PCa who underwent radical prostatectomy (n = 40, n = 56) and benign prostate hyperplasia (BPH...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124373
更新日期：2020-06-19 00:00:00
abstract：:HD-Zip proteins are unique to plants, and contain a homeodomain closely linked to a leucine zipper motif, which are involved in dimerization and DNA binding. Based on homology in the HD-Zip domain, gene structure and the presence of additional motifs, HD-Zips are divided into four families, HD-Zip I⁻IV. Phylogenetic a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19124047
更新日期：2018-12-14 00:00:00
abstract：:Overexpression of Sonic Hedgehog signaling (Shh) pathway molecules is associated with invasiveness and recurrence in breast carcinoma. Therefore, inhibition of the Shh pathway downstream molecule Glioma-associated Oncogene Homolog (Gli) was investigated for its ability to reduce progression and invasiveness of patient...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19051375
更新日期：2018-05-05 00:00:00
abstract：:Trichoderma reesei is a biotechnologically important filamentous fungus with the remarkable ability to secrete large amounts of enzymes, whose production is strongly affected by both the carbon and nitrogen sources. While the carbon metabolism regulators are extensively studied, the regulation of enzyme production by ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20174100
更新日期：2019-08-22 00:00:00
abstract：:Reduced low-density lipoprotein (LDL) cholesterol level is a characteristic feature of dyslipidemia in chronic hepatitis C virus (HCV) infection. However, abnormality in serum triglyceride (TG) has not been fully investigated. To clarify the impact of HCV genotype 1b (G1b) infection and advanced fibrosis on serum TG p...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160920576
更新日期：2015-08-31 00:00:00
