Abstract:
:Autophagy is an important process by which pathogens and damaged or unused organelles are eliminated. The role of autophagy in development and the immune response to pathogens is well established. Autophagy-related protein 8 (Atg8) is involved in the formation of the autophagosome and, with the help of the serine protease Atg4, mediates the delivery of both vesicles and the autophagosome to the vacuole. Here, we cloned the Aedes albopictus autophagy-related protein 8 (AaAtg8) gene and characterized its role in the innate immunity of the mosquito against microbial infections. AaAtg8 is comprised of an open reading frame (ORF) region of 357 bp encoding a polypeptide of 118 amino acid residues. A domain analysis of AaAtg8 revealed an Atg8 ubiquitin-like domain, Atg7/Atg4 interaction sites, and peptide binding sites. The AaAtg8 mRNA expression was high in the Malpighian tubules and heads of both sugar-fed and blood-fed adult female mosquitoes. The expression level of AaAtg8 mRNA increased in the midgut and abdominal carcass following being challenged with Listeria monocytogenes. To investigate the role of AaAtg8 in the innate immune responses of Ae. albopictus, AaAtg8 gene-silenced adult mosquitoes were challenged by injection or by being fed microorganisms in blood. High mortality rates were observed in mosquitoes in which AaAtg8 was silenced after challenges of microorganisms to the host by blood feeding. This suggests that Atg8-autophagy plays a critical role in the gut immunity in Ae. albopictus.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Kim CE,Park KB,Ko HJ,Keshavarz M,Bae YM,Kim B,Patnaik BB,Jang HA,Lee YS,Han YS,Jo YHdoi
10.3390/ijms21082944subject
Has Abstractpub_date
2020-04-22 00:00:00issue
8issn
1422-0067pii
ijms21082944journal_volume
21pub_type
杂志文章abstract::Vulvar squamous cell carcinoma (VSCC) originates from the progression of either a high-grade squamous intraepithelial lesion (HSIL) or differentiated-type vulvar intraepithelial neoplasia (dVIN), often in a background of lichen sclerosus (LS). The mechanisms leading to the progression of these premalignant lesions to ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21144880
更新日期:2020-07-10 00:00:00
abstract::The WRKY transcription factor superfamily is known to participate in plant growth and stress response. However, the role of this family in wheat (Triticum aestivum L.) is largely unknown. Here, a salt-induced gene TaWRKY13 was identified in an RNA-Seq data set from salt-treated wheat. The results of RT-qPCR analysis s...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20225712
更新日期:2019-11-14 00:00:00
abstract::Glucose variability (GV) has been recognized recently as a promoter of complications and therapeutic targets in diabetes. The aim of this study was to reconstruct and analyze gene networks related to GV in diabetes and its complications. For network analysis, we used the ANDSystem that provides automatic network recon...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21228691
更新日期:2020-11-18 00:00:00
abstract::Interferons (IFNs) are very powerful cytokines, which play a key role in combatting pathogen infections by controlling inflammation and immune response by directly inducing anti-pathogen molecular countermeasures. There are three classes of IFNs: type I, type II and type III. While type II IFN is specific for immune c...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20061445
更新日期:2019-03-21 00:00:00
abstract::Renal proximal tubular epithelial cells are significantly damaged during acute kidney injury. Renal proximal tubular cell-specific autophagy-deficient mice show increased sensitivity against renal injury, while showing few pathological defects under normal fed conditions. Considering that autophagy protects the proxim...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21010155
更新日期:2019-12-25 00:00:00
abstract::Cynoglossus semilaevis is an important economic fish species and has long been cultivated in China. Since the completion of its genome and transcriptome sequencing, genes relating to C. semilaevis development have been extensively studied. R-spondin 3 (Rspo3) is a member of the R-spondin family. It plays an important ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19071915
更新日期:2018-06-29 00:00:00
abstract::The physiological function of Arabidopsis thaliana universal stress protein (AtUSP) in plant has remained unclear. Thus, we report here the functional role of the Arabidopsis universal stress protein, AtUSP (At3g53990). To determine how AtUSP affects physiological responses towards cold stress, AtUSP overexpression (A...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18122546
更新日期:2017-11-27 00:00:00
abstract::Balanced osteoclast and osteoblast activity is necessary for skeletal health, whereas unbalanced osteoclast activity causes bone loss in many skeletal conditions. A better understanding of pathways that regulate osteoclast differentiation and activity is necessary for the development of new therapies to better manage ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21031056
更新日期:2020-02-05 00:00:00
abstract::Matrix metalloproteinase-1 (MMP-1) is one of the most widely studied enzymes involved in collagen degradation. Mutations of specific residues in the MMP-1 hemopexin-like (HPX) domain have been shown to modulate activity of the MMP-1 catalytic (CAT) domain. In order to reveal the structural and conformational effects o...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17101727
更新日期:2016-10-14 00:00:00
abstract::The mammalian target of rapamycin (mTOR) is the central regulator of mammalian cell growth, and is essential for the formation of two structurally and functionally distinct complexes: mTORC1 and mTORC2. mTORC1 can sense multiple cues such as nutrients, energy status, growth factors and hormones to control cell growth ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17101636
更新日期:2016-09-29 00:00:00
abstract::Paraoxonase 1 gene (PON1) polymorphisms and dietary vegetable and fruit intake are both established determinants of ischemic stroke (IS). However, little is known about whether these factors jointly influence the risk of IS. We analyzed the main effects of PON1, as well as the interactions between PON1 and dietary veg...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18122652
更新日期:2017-12-07 00:00:00
abstract::Transcription factor binding sites (TFBSs) play an important role in gene expression regulation. Many computational methods for TFBS prediction need sufficient labeled data. However, many transcription factors (TFs) lack labeled data in cell types. We propose a novel method, referred to as DANN_TF, for TFBS prediction...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20143425
更新日期:2019-07-12 00:00:00
abstract::β-lactam antibiotics are among the most important and widely used antimicrobials worldwide and are comprised of a large family of compounds, obtained by chemical modifications of the common scaffolds. Usually these modifications include the addition of active groups, but less frequently, molecules were synthesized in ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms22020617
更新日期:2021-01-09 00:00:00
abstract::As p300-mediated RelA/p65 hyperacetylation by signal transducers and activators of transcription 3 (STAT3) is critical for NF-κB activation, in the current study, the apoptotic mechanism of lambertianic acid (LA) was explored in relation to STAT3 phosphorylation and RelA/p65 acetylation in MCF-7, DU145, PC-3, and MDA-...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20122993
更新日期:2019-06-19 00:00:00
abstract::Oral biofilms attach onto both teeth surfaces and dental material surfaces in oral cavities. In the meantime, oral biofilms are not only the pathogenesis of dental caries and periodontitis, but also secondary caries and peri-implantitis, which would lead to the failure of clinical treatments. The material surfaces exp...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19103157
更新日期:2018-10-14 00:00:00
abstract::Microglia are the resident macrophages of the central nervous system (CNS) and play an important role in neuronal recovery by scavenging damaged neurons. However, overactivation of microglia leads to neuronal death that is associated with CNS disorders. Therefore, regulation of microglial activation has been suggested...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150915512
更新日期:2014-09-02 00:00:00
abstract::That the nervous system is the main target of lead (Pb) has long been considered an established fact until recent evidence has linked the Pb effect on the immune system to the toxic effects of Pb on the nervous system. In this paper, we present recent literature reports on the effect of Pb on the inflammatory processe...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17122140
更新日期:2016-12-19 00:00:00
abstract::The Duchenne muscular dystrophy (DMD) gene has a complex expression pattern regulated by multiple tissue-specific promoters and by alternative splicing (AS) of the resulting transcripts. Here, we used an RNAi-based approach coupled with DMD-targeted RNA-seq to identify RNA-binding proteins (RBPs) that regulate splicin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21207803
更新日期:2020-10-21 00:00:00
abstract::Growing interest and recent evidence have identified long non-coding RNA (lncRNA) as the potential regulatory elements for eukaryotes. LncRNAs can activate various transcriptional and post-transcriptional events that impact cellular functions though multiple regulatory functions. Recently, a large number of lncRNAs ha...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21082659
更新日期:2020-04-11 00:00:00
abstract::Autoimmune diabetes is a complex multifactorial disease with genetic and environmental factors playing pivotal roles. While many genes associated with the risk of diabetes have been identified to date, the mechanisms by which external triggers contribute to the genetic predisposition remain unclear. Here, we derived e...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165743
更新日期:2020-08-11 00:00:00
abstract::Calcium carbonate, especially with nanostructure, has been considered as a good candidate material for bone regeneration due to its excellent biodegradability and osteoconductivity. In this study, rod-like calcium carbonate nanoparticles (Rod-CC NPs) with desired water dispersibility were achieved with the regulation ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17050639
更新日期:2016-05-06 00:00:00
abstract::Aquaporins (AQPs) play a pivotal role in gut homeostasis since their distribution and function is modulated both in physiological and in pathophysiological conditions. The transport of water and solutes through gut epithelia is essential for osmoregulation and digestive and absorptive functions. This passage is regula...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17081213
更新日期:2016-07-27 00:00:00
abstract::To investigate the potential for the utilization of cotton stalk, ammonia hydrothermal treatment was applied to fractionate the samples into aqueous ammonia-soluble and ammonia-insoluble portions. The ammonia-soluble portion was purified to yield lignin fractions. The lignin fractions obtained were characterized by we...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms131115209
更新日期:2012-11-16 00:00:00
abstract::Cholesteatoma is a destructive and expanding growth of keratinizing squamous epithelium in the middle ear or petrous apex. The molecular and cellular processes of the pathogenesis of acquired middle ear cholesteatoma have not been fully understood. In this study, comparative proteomic analysis was conducted to investi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms140714439
更新日期:2013-07-11 00:00:00
abstract::Catalytic decomposition of sucrose by acid invertases (AINVs) under acidic conditions plays an important role in the development of sink organs in plants. To reveal the function of AINVs in the development of pepper fruits, nine AINV genes of pepper were identified. Protein sequencing and phylogenetic analysis reveale...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20010015
更新日期:2018-12-20 00:00:00
abstract::Pancreatic cancer (PC) is a highly lethal malignancy due to the cancer routinely being diagnosed late and having a limited response to chemotherapy. Pancreatic ductal adenocarcinoma (PDAC) is the most common form of pancreatic malignant tumor, representing more than 85% of all pancreatic cancers. In the present study,...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21124473
更新日期:2020-06-23 00:00:00
abstract::The aim of this study was to explore the optimal therapeutic dose and time window of picroside II for treating cerebral ischemic injury in rats according to the orthogonal test. The middle cerebral artery occlusion (MCAO) models were established by intraluminally inserting a thread into middle cerebral artery (MCA) fr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13032551
更新日期:2012-01-01 00:00:00
abstract::In this study, caviar (sturgeon eggs) was used to elucidate its roles in adiponectin production and skin anti-aging. Recently, caviar has been largely used not only as a nutritional food, but also in cosmetic products. In particular, it has been reported that docosahexaenoic acid (DHA), as one of the main phospholipid...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21093383
更新日期:2020-05-11 00:00:00
abstract::Protein splicing catalyzed by inteins utilizes many different combinations of amino-acid types at active sites. Inteins have been classified into three classes based on their characteristic sequences. We investigated the structural basis of the protein splicing mechanism of class 3 inteins by determining crystal struc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21218367
更新日期:2020-11-07 00:00:00
abstract::Acute lung injury is characterized by the infiltration of neutrophils into lungs and the subsequent impairment of lung function. Here we explored the role of TDAG8 in lung injury induced by lipopolysaccharide (LPS) administrated intratracheally. In this model, cytokines and chemokines released from resident macrophage...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms161226145
更新日期:2015-12-04 00:00:00