Abstract:
:Acute lung injury is characterized by the infiltration of neutrophils into lungs and the subsequent impairment of lung function. Here we explored the role of TDAG8 in lung injury induced by lipopolysaccharide (LPS) administrated intratracheally. In this model, cytokines and chemokines released from resident macrophages are shown to cause neutrophilic inflammation in the lungs. We found that LPS treatment increased TDAG8 expression in the lungs and confirmed its expression in resident macrophages in bronchoalveolar lavage (BAL) fluids. LPS administration remarkably increased neutrophil accumulation without appreciable change in the resident macrophages, which was associated with increased penetration of blood proteins into BAL fluids, interstitial accumulation of inflammatory cells, and damage of the alveolar architecture. The LPS-induced neutrophil accumulation and the associated lung damage were enhanced in TDAG8-deficient mice as compared with those in wild-type mice. LPS also increased several mRNA and protein expressions of inflammatory cytokines and chemokines in the lungs or BAL fluids. Among these inflammatory mediators, mRNA and protein expression of KC (also known as CXCL1), a chemokine of neutrophils, were significantly enhanced by TDAG8 deficiency. We conclude that TDAG8 is a negative regulator for lung neutrophilic inflammation and injury, in part, through the inhibition of chemokine production.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Tsurumaki H,Mogi C,Aoki-Saito H,Tobo M,Kamide Y,Yatomi M,Sato K,Dobashi K,Ishizuka T,Hisada T,Yamada M,Okajima Fdoi
10.3390/ijms161226145subject
Has Abstractpub_date
2015-12-04 00:00:00pages
28931-42issue
12issn
1422-0067pii
ijms161226145journal_volume
16pub_type
杂志文章abstract::Src is the representative member of the Src-family kinases (SFKs), a group of tyrosine kinases involved in several cellular processes. Its main function has been for long confined to the plasma membrane/cytoplasm compartment, being a myristoylated protein anchored to the cell membrane and functioning downstream to rec...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21082675
更新日期:2020-04-11 00:00:00
abstract::The application of combined nitrogen, especially nitrate, to soybean plants is known to strongly inhibit nodule formation, growth and nitrogen fixation. In the present study, we measured the effects of supplying 5 mM nitrate on the growth of nodules, primary root, and lateral roots under light at 28 °C or dark at 18 °...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15034464
更新日期:2014-03-13 00:00:00
abstract::A whole-bacterium-based SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure was adopted in this study for the selection of an ssDNA aptamer that binds to Bifidobacterium bifidum. After 12 rounds of selection targeted against B. bifidum, 30 sequences were obtained and divided into seven families...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18050883
更新日期:2017-04-25 00:00:00
abstract::Cultured human primary cells have a limited lifespan undergoing dedifferentiation or senescence. Anterior cruciate ligaments (ACL) are hypocellular but tissue engineering (TE) requires high cell numbers. Simian virus (SV) 40 tumor (T) antigen expression could extend the lifespan of cells. This study aimed to identify ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21020593
更新日期:2020-01-16 00:00:00
abstract::Gastrointestinal stromal tumors (GIST) carrying the D842V activating mutation in the platelet-derived growth factor receptor alpha (PDGFRA) gene are a very rare subgroup of GIST (about 10%) known to be resistant to conventional tyrosine kinase inhibitors (TKIs) and to show an indolent behavior. In this study, we perfo...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19030732
更新日期:2018-03-04 00:00:00
abstract::Protein folding, misfolding and aggregation, as well as the way misfolded and aggregated proteins affects cell viability are emerging as key themes in molecular and structural biology and in molecular medicine. Recent advances in the knowledge of the biophysical basis of protein folding have led to propose the energy ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms9122515
更新日期:2008-12-01 00:00:00
abstract::Kidney renal cell carcinoma (KIRC), which is the most common subtype of kidney cancer, has a poor prognosis and a high mortality rate. In this study, a multi-omics analysis is performed to build a multi-gene prognosis signature for KIRC. A combination of a DNA methylation analysis and a gene expression data analysis r...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20225720
更新日期:2019-11-14 00:00:00
abstract::Patients with chronic hepatitis C virus (HCV) develop hepatocellular carcinoma (HCC) regardless of achieving a sustained viral response (SVR). Because advanced liver fibrosis is a powerful risk factor for HCC, we analyzed the association between autotaxin (ATX), a liver fibrosis marker, and post-SVR HCC development wi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21124517
更新日期:2020-06-25 00:00:00
abstract::We herein review available computational and experimental data pointing to the abundance of structural disorder within the nucleoprotein (N) and phosphoprotein (P) from three paramyxoviruses, namely the measles (MeV), Nipah (NiV) and Hendra (HeV) viruses. We provide a detailed molecular description of the mechanisms g...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160715688
更新日期:2015-07-10 00:00:00
abstract::Benign prostate hyperplasia (BPH) is one of the most common urological problems in mid-aged to elderly men. Risk factors of BPH include family history, obesity, type 2 diabetes, and high oxidative stress. The main medication classes for BPH management are alpha blockers and 5α-reductase inhibitors. However, these conv...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19041137
更新日期:2018-04-10 00:00:00
abstract::Both genetic and epigenetic alterations can control the progression of cancer. Genetic alterations are impossible to reverse, while epigenetic alterations are reversible. This advantage suggests that epigenetic modifications should be preferred in therapy applications. DNA methyltransferases and histone deacetylases h...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms12074465
更新日期:2011-01-01 00:00:00
abstract::Abscisic acid (ABA) plays important roles in positively or negatively regulating plant disease resistance to pathogens. Here, we reassess the role of endogenous and exogenous ABA by using: 35S::ABA2, a previously reported transgenic Arabidopsis line with increased endogenous ABA levels; aba2-1, a previously reported A...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20102544
更新日期:2019-05-23 00:00:00
abstract::Recently, extreme ultraviolet (EUV) radiation has been increasingly used to modify polymers. Properties such as the extremely short absorption lengths in polymers and the very strong interaction of EUV photons with materials may play a key role in achieving new biomaterials. The purpose of the study was to examine the...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249679
更新日期:2020-12-18 00:00:00
abstract::The market success of any rice cultivar is exceedingly dependent on its grain appearance, as well as its grain yield, which define its demand by consumers as well as growers. The present study was undertaken to explore the contribution of nine major genes, qPE9~1, GW2, SLG7, GW5, GS3, GS7, GW8, GS5, and GS2, in regula...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20194824
更新日期:2019-09-28 00:00:00
abstract::Circadian clock not only functions as a cellular time-keeping mechanism, but also acts as a master regulator to coordinate the tradeoff between plant growth and defense in higher plants by timing a few kinds of phytohormone biosynthesis and signaling, including jasmonic acid (JA). Notably, circadian clock and JA pathw...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20215254
更新日期:2019-10-23 00:00:00
abstract::We aimed to validate the effect of non-canonical splice site variants in the RPGR gene in five patients from four families diagnosed with retinitis pigmentosa. Four variants located in intron 2 (c.154 + 3_154 + 6del), intron 3 (c.247 + 5G>A), intron 7 (c.779-5T>G), and intron 13 (c.1573-12A>G), respectively, were anal...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22020850
更新日期:2021-01-16 00:00:00
abstract::Friedreich's ataxia (FA) is a trinucleotide repeats expansion neurodegenerative disorder, for which no cure or approved therapies are present. In most cases, GAA trinucleotide repetitions in the first intron of the FXN gene are the genetic trigger of FA, determining a strong reduction of frataxin, a mitochondrial prot...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21030916
更新日期:2020-01-30 00:00:00
abstract::Normal tissue toxicity is a dose-limiting factor in radiation therapy. Therefore, a detailed understanding of the normal tissue response to radiation is necessary to predict the risk of normal tissue toxicity and to development strategies for tissue protection. One component of normal tissue that is continuously expos...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21072336
更新日期:2020-03-27 00:00:00
abstract::A fundamental step for cell growth and differentiation is the cell adhesion. The purpose of this study was to determine the adhesion of different cell lineages, adipose derived stromal cells, osteoblasts, and gingival fibroblast to titanium and zirconia dental implants with different surface treatments. Primary cells ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21114110
更新日期:2020-06-09 00:00:00
abstract::Cyclosporin A (CsA), a calcineurin inhibitor, remain the cornerstone of immunosuppressive regimens, regardless of nephrotoxicity, which depends on the duration of drug exposure. The mechanisms and biomarkers underlying the transition from CsA-induced renal dysfunction to nephrotoxicity deserve better elucidation, and ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15058979
更新日期:2014-05-20 00:00:00
abstract::Insulin-resistance is one of the main factors responsible for the onset and progression of Metabolic Syndrome (MetS). Among all polyphenols, the effects of flavonoids and their main food sources on insulin sensitivity have been widely evaluated in molecular and clinical studies. The aim of this review is to analyse th...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20092061
更新日期:2019-04-26 00:00:00
abstract::The mosquito-borne viral disease caused by the Dengue virus is an expanding global threat. Diagnosis in low-resource-settings and epidemiological surveillance urgently requires new immunoprobes for serological tests. Structure-based epitope prediction is an efficient method to design diagnostic peptidic probes able to...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20081921
更新日期:2019-04-18 00:00:00
abstract::Stable five-coordinate Pt(II) complexes have been highlighted as a promising and original platform for the development of new cytotoxic drugs. Their interaction with proteins has been scarcely studied. Here, the reactivity of the five-coordinate Pt(II) compound [Pt(I)(Me) (dmphen)(olefin)] (Me = methyl, dmphen = 2,9-d...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20030520
更新日期:2019-01-26 00:00:00
abstract::Macrophages can polarize into two different states (M1 and M2), which play contrasting roles during pathogenesis or tissue damage. M1 polarized macrophages produce pro-inflammatory cytokines and mediators resulting in inflammation, while M2 macrophages have an anti-inflammatory effect. Secretion of appropriate cytokin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19082208
更新日期:2018-07-28 00:00:00
abstract::Pneumonia is a cause of high morbidity and mortality in humans. Animal models are indispensable to investigate the complex cellular interactions during lung injury and repair in vivo. The time sequence of lesion development and regeneration is described after endobronchial inoculation of calves with Chlamydia psittaci...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21082817
更新日期:2020-04-17 00:00:00
abstract::The immune system and the central nervous system message each other to preserving central homeostasis. Both systems undergo changes during aging that determine central age-related defects. Ellagic acid (EA) is a natural product which is beneficial in both peripheral and central diseases, including aging. We analyzed t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21103631
更新日期:2020-05-21 00:00:00
abstract::Timely and reliable distinction of sepsis from non-infectious systemic inflammatory response syndrome (SIRS) supports adequate antimicrobial therapy and saves lives but is clinically challenging. Blood transcriptional profiling promises to deliver insights into the pathomechanisms of SIRS and sepsis and to accelerate ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20092290
更新日期:2019-05-09 00:00:00
abstract::Due to the addressability and programmability, DNA has been applied not merely in constructing static elegant nanostructures such as two dimensional and three dimensional DNA nanostructures but also in designing dynamic nanodevices. Moreover, DNA could combine with hydrophobic organic molecules to be a new amphiphilic...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19082283
更新日期:2018-08-03 00:00:00
abstract::Hyperpolarization-activated cationic HCN channels comprise four members (HCN1-4) that control dendritic integration, synaptic transmission and action potential firing. In the kidney, HCN1, HCN2 and HCN3 are differentially expressed and contribute to the transport of sodium, potassium (K+) and ammonium into the nephron...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20204995
更新日期:2019-10-09 00:00:00
abstract::Fibroblast growth factor 21 (FGF-21) is known to be a biomarker for mitochondrial disorders. An upregulation of FGF-21 in serum and muscle of carnitine palmitoyltransferase I (CPT I) and carnitine palmitoyltransferase II (CPT II) knock-out mice has been reported. In human CPT II deficiency, enzyme activity and protein...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20061400
更新日期:2019-03-20 00:00:00