Abstract:
:The Wnt/β-catenin pathway plays an essential role in the tumorigenesis of colorectal cancer. T-cell factor-4 (TCF4) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors, and dysregulation of β-catenin is decisive for the initiation and progression of colorectal cancer. However, the role of TCF4 in the transcriptional regulation of its target gene remained poorly understood. Resveratrol is a dietary phytoalexin and present in many plants, including grape skin, nuts and fruits. Although resveratrol has been widely implicated in anti-tumorigenic and pro-apoptotic properties in several cancer models, the underlying cellular mechanisms are only partially understood. The current study was performed to elucidate the molecular mechanism of the anti-cancer activity of resveratrol in human colorectal cancer cells. The treatment of resveratrol and other phytochemicals decreased the expression of TCF4. Resveratrol decreases cellular accumulation of exogenously-introduced TCF4 protein, but did not change the TCF4 transcription. The inhibition of proteasomal degradation using MG132 (carbobenzoxy-Leu-Leu-leucinal) and lactacystin ameliorates resveratrol-stimulated down-regulation of TCF4. The half-life of TCF4 was decreased in the cells exposed to resveratrol. Resveratrol increased phosphorylation of TCF4 at serine/threonine residues through ERK (extracellular signal-regulated kinases) and p38-dependent pathways. The TCF4 knockdown decreased TCF/β-catenin-mediated transcriptional activity and sensitized resveratrol-induced apoptosis. The current study provides a new mechanistic link between resveratrol and TCF4 down-regulation and significant benefits for further preclinical and clinical practice.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Jeong JB,Lee J,Lee SHdoi
10.3390/ijms160510411subject
Has Abstractpub_date
2015-05-07 00:00:00pages
10411-25issue
5issn
1422-0067pii
ijms160510411journal_volume
16pub_type
杂志文章abstract::Roots are vital to plant survival and crop yield, yet few efforts have been made to characterize the expressed genes in the roots of non-model plants (root transcriptomes). This study was conducted to sequence, assemble, annotate, and characterize the root transcriptomes of three caladium cultivars (Caladium × hortula...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18040712
更新日期:2017-03-27 00:00:00
abstract::Dietary fats and sugars were identified as risk factors for overweight and neurodegeneration, especially in middle-age, an earlier stage of the aging process. Therefore, our aim was to study the metabolic response of both white adipose tissue and brain in middle aged rats fed a typical Western diet (high in saturated ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21030786
更新日期:2020-01-25 00:00:00
abstract::Quantitative understanding of the mechanical behavior of biological liquid crystals such as proteins is essential for gaining insight into their biological functions, since some proteins perform notable mechanical functions. Recently, single-molecule experiments have allowed not only the quantitative characterization ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms10094009
更新日期:2009-09-10 00:00:00
abstract::We investigated the prevalence of reported deep-intronic variants in a French cohort of 70 patients with Stargardt disease harboring a monoallelic pathogenic variant on the exonic regions of ABCA4. Direct Sanger sequencing of selected intronic regions of ABCA4 was conducted. Complete phenotypic analysis and correlatio...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20205053
更新日期:2019-10-11 00:00:00
abstract::Drought stress is one of the most serious threats to cucumber quality and yield. To gain a good understanding of the molecular mechanism upon water deficiency, we compared and analyzed the RNA sequencing-based transcriptomic responses of two contrasting cucumber genotypes, L-9 (drought-tolerant) and A-16 (drought-sens...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19072067
更新日期:2018-07-16 00:00:00
abstract::When leaves receive excess light energy, excess reductants accumulate in chloroplasts. It is suggested that some of the reductants are oxidized by the mitochondrial respiratory chain. Alternative oxidase (AOX), a non-energy conserving terminal oxidase, was upregulated in the photosynthetic mutant of Arabidopsis thalia...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20123067
更新日期:2019-06-23 00:00:00
abstract::The use of herbicides is an effective and economic way to control weeds, but their availability for rapeseed is limited due to the shortage of herbicide-resistant cultivars in China. The single-point mutation in the acetohydroxyacidsynthase (AHAS) gene can lead to AHAS-inhibiting herbicide resistance. In this study, t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041345
更新日期:2020-02-17 00:00:00
abstract::Superparamagnetic iron oxide nanoparticles (SPIONs) have attracted increasing attention in many biomedical fields. In magnetic drug targeting SPIONs are injected into a tumour supplying artery and accumulated inside the tumour with a magnet. The effectiveness of this therapy is thus dependent on magnetic properties, s...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16059368
更新日期:2015-04-24 00:00:00
abstract::In salt-sensitive hypertension, reactive oxygen species (ROS) play a major role in the progression of renal disease partly through the activation of the mineralocorticoid receptor (MR). We have previously demonstrated that urinary vanin-1 is an early biomarker of oxidative renal tubular injury. However, it remains unk...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20184481
更新日期:2019-09-11 00:00:00
abstract::Preterm birth (PTB) can be defined as the endpoint of a complex process that could be influenced by maternal and environmental factors. Epigenetics recently emerged as an interesting field of investigation since it represents an important mechanism of regulation. This study evaluates epigenetic impact of preterm birth...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21145044
更新日期:2020-07-17 00:00:00
abstract::Non-melanomatous skin cancers (NMSCs), which include basal and squamous cell carcinoma (BCC and SCC respectively), represent a significant burden on the population, as well as an economic load to the health care system; yet treatments of these preventable cancers remain ineffective. Studies estimate that there has bee...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18071369
更新日期:2017-06-27 00:00:00
abstract::Resveratrol, the phenolic substance isolated initially from Veratrum grandiflorum and richly present in grapes, wine, peanuts, soy, and berries, has been attracting attention of scientists and medical doctors for many decades. Herein, we review its effects on the vascular system. Studies utilizing cell cultures and pr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20071523
更新日期:2019-03-27 00:00:00
abstract::Research on the protein folding problem differentiates the protein folding process with respect to the duration of this process. The current structure encoded in sequence dogma seems to be clearly justified, especially in the case of proteins referred to as fast-folding, ultra-fast-folding or downhill. In the present ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21207632
更新日期:2020-10-15 00:00:00
abstract::Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is the main therapeutic strategy for patients with both malignant and nonmalignant disorders. The therapeutic benefits of allo-HSCT in malignant disorders are primarily derived from the graft-versus-leukemia (GvL) effect, in which T cells in the donor graf...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21124281
更新日期:2020-06-16 00:00:00
abstract::Nonylphenol (NP) is a degradation product of nonylphenol polyethoxylates, which are widely used in the production of industrial and consumer surfactants. The aim of the present study was to evaluate the effect of NP on the antioxidant capacity and cognitive ability of mice. NP was given orally by gavages at doses of 0...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12010114
更新日期:2010-12-30 00:00:00
abstract::Polyphenol compositions and concentrations in skins and seeds of five muscadine grapes (cv. "Noble", "Alachua", "Carlos", "Fry", and "Granny Val") cultivated in the United States (Tallahassee-Florida, TA-FL) and South China (Nanning-Guangxi, NN-GX and Pu'er-Yunnan, PE-YN) were investigated, using ultra performance liq...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18030631
更新日期:2017-03-14 00:00:00
abstract::Reproductive dysfunction and malignancies related to the male gender represent a serious health concern, whose incidence has significantly risen over the past years. Prior to treatment, testicular or prostate cancer patients often display poor semen characteristics similar to subfertile or infertile patients. This fac...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms16047230
更新日期:2015-03-31 00:00:00
abstract::The recombinant baculovirus has been widely used as an efficient tool to mediate gene delivery into mammalian cells but has barely been used in fish cells. In the present study, we constructed a recombinant baculovirus containing the dual-promoter cytomegalovirus (CMV) and white spot syndrome virus (WSSV) immediate-ea...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19123767
更新日期:2018-11-27 00:00:00
abstract::In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen. Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are wel...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21176154
更新日期:2020-08-26 00:00:00
abstract::Busulfan is an alkylating agent routinely used in conditioning regimens prior to allogeneic hematopoietic cell transplantation (HCT) for various nonmalignant disorders, including inborn errors of metabolism. The combination of model-based dosing and therapeutic drug monitoring (TDM) of busulfan pharmacokinetics (PK) t...
journal_title:International journal of molecular sciences
pub_type:
doi:10.3390/ijms21165634
更新日期:2020-08-06 00:00:00
abstract::Hypoxia is a common hallmark of solid tumors and is associated with aggressiveness, metastasis and poor outcome. Cancer cells under hypoxia undergo changes in metabolism and there is an intense crosstalk between cancer cells and cells from the tumor microenvironment. This crosstalk is facilitated by small extracellula...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21145071
更新日期:2020-07-17 00:00:00
abstract::Melatonin is produced by the pineal gland. It can be regarded as an anticancer agent and used for combined therapy, owing to its oncostatic, antioxidant, and immunoregulatory activities. Retinoic acid is widely used for the treatment of acute promyelocytic leukemia; however, it has adverse effects on the human organis...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19102873
更新日期:2018-09-21 00:00:00
abstract::The imbalance between cell proliferation and apoptosis can lead to tumor progression, causing oncogenic transformation, abnormal cell proliferation and cell apoptosis suppression. Tea polysaccharide (TPS) is the major bioactive component in green tea, it has showed antioxidant, antitumor and anti-inflammatory bioactiv...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19020506
更新日期:2018-02-08 00:00:00
abstract::Protein hydroxylation is one type of post-translational modifications (PTMs) playing critical roles in human diseases. It is known that protein sequence contains many uncharacterized residues of proline and lysine. The question that needs to be answered is: which residue can be hydroxylated, and which one cannot. The ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19092817
更新日期:2018-09-18 00:00:00
abstract::Decades of study on the role of mitochondria in living cells have evidenced the importance of the 18 kDa mitochondrial translocator protein (TSPO), first discovered in the 1977 as an alternative binding site for the benzodiazepine diazepam in the kidneys. This protein participates in a variety of cellular functions, i...
journal_title:International journal of molecular sciences
pub_type: 社论
doi:10.3390/ijms18122640
更新日期:2017-12-06 00:00:00
abstract::The juvenile hormone (JH) signalling and ecdysone signalling pathways are crucial endocrine signalling pathways that orchestrate the metamorphosis of insects. The metamorphic process, the morphological change from the immature to adult forms, is orchestrated by the dramatic reduction of JH and downstream transcription...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20102431
更新日期:2019-05-16 00:00:00
abstract::Adult Renal Stem/Progenitor Cells (ARPCs) have been recently identified in the human kidney and several studies show their active role in kidney repair processes during acute or chronic injury. However, little is known about their immunomodulatory properties and their capacity to regulate specific T cell subpopulation...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010274
更新日期:2020-12-29 00:00:00
abstract::Tomato cell wall-associated kinase 1 (SlWAK1) has only been studied in biotic stress response and hence its function in abiotic stress remains unknown. In a screening under salinity of an insertional mutant collection of tomato (Solanum lycopersicum L.), a mutant exhibiting lower degree of leaf chlorosis than wild typ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21176308
更新日期:2020-08-31 00:00:00
abstract::Claudins regulate paracellular permeability in different tissues. The claudin-binding domain of Clostridium perfringens enterotoxin (cCPE) is a known modulator of a claudin subset. However, it does not efficiently bind to claudin-1 (Cldn1). Cldn1 is a pharmacological target since it is (i) an essential co-receptor for...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20194774
更新日期:2019-09-26 00:00:00
abstract::Intraluminal vesicles (ILVs) are released into the extracellular space as exosomes after the fusion of multivesicular bodies (MVBs) with the plasma membrane. miRNAs are delivered to the raft-like region of MVB by RNA-binding proteins (RBPs). RNA loading into exosomes can be either through direct interaction between RN...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21228503
更新日期:2020-11-12 00:00:00