Abstract：
:The translation of metallothioneins (MTs) is one of the defense strategies by which organisms protect themselves from metal-induced toxicity. MTs belong to a family of proteins comprising MT-1, MT-2, MT-3, and MT-4 classes, with multiple isoforms within each class. The main aim of this study was to determine the behavior of MT in dependence on various externally modelled environments, using electrochemistry. In our study, the mass distribution of MTs was characterized using MALDI-TOF. After that, adsorptive transfer stripping technique with differential pulse voltammetry was selected for optimization of electrochemical detection of MTs with regard to accumulation time and pH effects. Our results show that utilization of 0.5 M NaCl, pH 6.4, as the supporting electrolyte provides a highly complicated fingerprint, showing a number of non-resolved voltammograms. Hence, we further resolved the voltammograms exhibiting the broad and overlapping signals using curve fitting. The separated signals were assigned to the electrochemical responses of several MT complexes with zinc(II), cadmium(II), and copper(II), respectively. Our results show that electrochemistry could serve as a great tool for metalloproteomic applications to determine the ratio of metal ion bonds within the target protein structure, however, it provides highly complicated signals, which require further resolution using a proper statistical method, such as curve fitting.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Merlos Rodrigo MA,Molina-López J,Jimenez Jimenez AM,Planells Del Pozo E,Adam P,Eckschlager T,Zitka O,Richtera L,Adam Vdoi
10.3390/ijms18030610subject
Has Abstractpub_date
2017-03-11 00:00:00issue
3issn
1422-0067pii
ijms18030610journal_volume
18pub_type
杂志文章abstract：:Ganoderma lucidum polysaccharide (GLP) is a biologically active substance reported to possess anti-tumor ability. Nonetheless, the mechanisms of GLP-stimulated apoptosis are still unclear. This study aims to determine the inhibitory and apoptosis-inducing effects of GLP on HCT-116 cells. We found that GLP reduced cell...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15059103
更新日期：2014-05-22 00:00:00
abstract：:Despite the promising pharmacological properties of curcumin, the transport and effective release of curcumin is still a challenge. The advances in functionalized nanocarriers for curcumin have also been motivated by the anticancer activity of this natural compound, aiming at targeted therapies. Here, stealth (aqueous...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21103641
更新日期：2020-05-21 00:00:00
abstract：:The immune system and the central nervous system message each other to preserving central homeostasis. Both systems undergo changes during aging that determine central age-related defects. Ellagic acid (EA) is a natural product which is beneficial in both peripheral and central diseases, including aging. We analyzed t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21103631
更新日期：2020-05-21 00:00:00
abstract：:The maintenance of ordinal cell cycle phases is a critical biological process in cancer genesis, which is a crucial target for anti-cancer drugs. As an important natural isoquinoline alkaloid from Chinese herbal medicine, Berberine (BBR) has been reported to possess anti-cancer potentiality to induce cell cycle arrest...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19020327
更新日期：2018-01-23 00:00:00
abstract：:The adaptation of adenoviruses as gene delivery tools has resulted in the development of high-capacity adenoviral vectors (HC-AdVs), also known, helper-dependent or "gutless". Compared with earlier generations (E1/E3-deleted vectors), HC-AdVs retain relevant features such as genetic stability, remarkable efficacy of i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21103643
更新日期：2020-05-21 00:00:00
abstract：:Stem cells, a special subset of cells derived from embryo or adult tissues, are known to present the characteristics of self-renewal, multiple lineages of differentiation, high plastic capability, and long-term maintenance. Recent reports have further suggested that neural stem cells (NSCs) derived from the adult hipp...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms11052039
更新日期：2010-05-05 00:00:00
abstract：:NAC (NAM/ATAF/CUC) transcription factors comprise a large plant-specific gene family that contains more than 149 members in rice. Extensive studies have revealed that NAC transcription factors not only play important roles in plant growth and development, but also have functions in regulation of responses to biotic an...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms16024306
更新日期：2015-02-17 00:00:00
abstract：:Curcumin diglutaric acid (CurDG), an ester prodrug of curcumin, has the potential to be developed as an anti-inflammatory agent due to its improved solubility and stability. In this study, the anti-inflammatory effects of CurDG were evaluated. The effects of CurDG on inflammatory mediators were evaluated in LPS-stimul...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21165700
更新日期：2020-08-09 00:00:00
abstract：:Glutathione synthetases catalyze the ATP-dependent synthesis of glutathione from l-γ-glutamyl- l-cysteine and glycine. Although these enzymes have been sequenced and characterized from a variety of biological sources, their exact catalytic mechanism is not fully understood and nothing is known about their adaptation a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12096312
更新日期：2011-01-01 00:00:00
abstract：:miRNAs are small noncoding RNAs capable of regulating gene expression at the post-transcriptional level. A growing body of evidence demonstrated that let-7 family of miRNAs, as one of the highly conserved miRNAs, plays an important role in cell differentiation and development, as well as tumor suppressor function depe...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms151019119
更新日期：2014-10-21 00:00:00
abstract：:Cystic fibrosis is an inherited multi-organ disorder caused by mutations in the CFTR gene. Patients with this disease exhibit characteristic abnormalities in the levels of unsaturated fatty acids in blood and tissue. Recent studies have uncovered an underlying biochemical mechanism for some of these changes, namely in...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms150916083
更新日期：2014-09-11 00:00:00
abstract：:Blood rheology is a key determinant of tissue perfusion at rest and during exercise. The present study investigated the effects of race distance on hematological, blood rheological, and red blood cell (RBC) senescence parameters. Eleven runners participated in the Martigny-Combes à Chamonix 40 km race (MCC, elevation ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228531
更新日期：2020-11-12 00:00:00
abstract：:Understanding the kinetics of peptide self-assembly is important because of the involvement of peptide amyloid fibrils in several neurodegenerative diseases. In this paper, we have studied the dissolution kinetics of self-assembled model peptide fibrils after a dilution quench. Due to the low concentrations involved, ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21207671
更新日期：2020-10-16 00:00:00
abstract：:A thrombotic occlusion of the vessel fed by ruptured coronary atherosclerotic plaque may result in unstable angina, myocardial infarction or death, whereas embolization from a plaque in carotid arteries may result in transient ischemic attack or stroke. The atherosclerotic plaque prone to such clinical events is terme...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms160510121
更新日期：2015-05-05 00:00:00
abstract：:Various sulfonamide derivatives are intensively studied as anticancer agents owing to their inhibitory activity against human tumor-associated carbonic anhydrase isoforms. In this work, different synthetic procedures for the series of 1,3,5-triazinyl-aminobenzenesulfonamide conjugates with amino acids, possessing pola...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21103661
更新日期：2020-05-22 00:00:00
abstract：:In this study, a surface chemical-modified rice husk biochar with abundant amino groups and disulfide bonds for the removal of cadmium was prepared using cystamine dihydrochloride as a modification ligand and glutaraldehyde as a crosslinker. The biochars were characterized by Fourier transform infrared spectrometry (F...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20071775
更新日期：2019-04-10 00:00:00
abstract：:Osteoporosis represents the most common bone disease worldwide and results in a significantly increased fracture risk. Extrinsic and intrinsic factors implicated in the development of osteoporosis are also associated with delayed fracture healing and impaired bone regeneration. Based on a steadily increasing life expe...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20010083
更新日期：2018-12-26 00:00:00
abstract：BACKGROUND:There are eighteen species within the Penicillium genus section chrysogena, including the original penicillin producers Penicillium notatum (Fleming strain) and Penicillium chrysogenum NRRL 1951. Other wild type isolates of the Penicillium genus are relevant for the production of useful proteins and primary ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21113936
更新日期：2020-05-30 00:00:00
abstract：:The last step in the biosynthesis of flavin adenine dinucleotide (FAD) is considered a target for the design of antimicrobial drugs because it is carried out by two non-homologous proteins in eukaryotic and prokaryotic organisms. Monofunctional FMN: adenylyltransferases (FMNAT) in Eukarya and FMNAT modules of bifuncti...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21103738
更新日期：2020-05-25 00:00:00
abstract：:Epigenetic modifications including DNA methylation, histone modifications, and chromatin remodeling are crucial regulators of chromatin architecture and gene expression in plants. Their dynamics are significantly influenced by oxidants, such as reactive oxygen species (ROS) and nitric oxide (NO), and antioxidants, lik...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21041419
更新日期：2020-02-19 00:00:00
abstract：:The overexpressing ABCB1 transporter is one of the key factors leading to multidrug resistance (MDR). Thus, many ABCB1 inhibitors have been found to be able to overcome ABCB1-mediated MDR. However, some inhibitors also work as a substrate of ABCB1, which indicates that in order to achieve an effective reversal dosage,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041387
更新日期：2020-02-19 00:00:00
abstract：:We report the characterization of 16 polymorphic microsatellite markers in the golden apple snail, Pomacea canaliculata, a pest registered in the list of "100 of the world's worst invasive alien species". The fast isolation by AFLP (Amplified Fragment Length Polymorphism) of sequences containing repeats (FIASCO) metho...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12095993
更新日期：2011-01-01 00:00:00
abstract：:A dodecadepsipeptide valinomycin (VLM) has been most recently reported to be a potential anti-coronavirus drug that could be efficiently produced on a large scale. It is thus of importance to study solid-phase forms of VLM in order to be able to ensure its polymorphic purity in drug formulations. The previously availa...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21144907
更新日期：2020-07-11 00:00:00
abstract：:The essential role of Hippo signaling pathway in cancer development has been elucidated by recent studies. In the gastrointestinal tissues, deregulation of the Hippo pathway is one of the most important driving events for tumorigenesis. It is widely known that Yes-associated protein 1 (YAP1) and WW domain that contain...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20071576
更新日期：2019-03-29 00:00:00
abstract：:Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates are known to tolerate superior quinolone antimicrobials compared with other antibacterial agents. Among the clones belonging to sequence type (ST) 131 by multilocus sequence typing, the involvement of the H30-Rx subclone has been reported worldwi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205162
更新日期：2019-10-18 00:00:00
abstract：:Due to the present scenario of climate change, plants have to evolve strategies to survive and perform under a plethora of biotic and abiotic stresses, which restrict plant productivity. Maintenance of plant protein functional conformation and preventing non-native proteins from aggregation, which leads to metabolic d...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20215321
更新日期：2019-10-25 00:00:00
abstract：:Increasing photosynthetic ability as a whole is essential for acquiring higher crop yields. Nonleaf green organs (NLGOs) make important contributions to photosynthate formation, especially under stress conditions. However, there is little information on the pod wall in legume forage related to seed development and yie...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124457
更新日期：2020-06-23 00:00:00
abstract：:Systemic sclerosis is an auto-immune disease characterized by skin involvement that often affects multiple organ systems. Pulmonary hypertension is a common finding that can significantly impact prognosis. Molecular pathophysiological mechanisms underlying pulmonary hypertension in systemic sclerosis can be extremely ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21124430
更新日期：2020-06-22 00:00:00
abstract：:Virus-like nanoparticles (VLNPs) have been studied extensively as nanocarriers for targeted drug delivery to cancer cells. However, VLNPs have intrinsic drawbacks, in particular, potential antigenicity and immunogenicity, which hamper their clinical applications. Thus, they can be eliminated easily and rapidly by host...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20194903
更新日期：2019-10-03 00:00:00
abstract：:Latent transforming growth factor-β-1 binding protein-2 (LTBP-2) belongs to the LTBP-fibrillin superfamily of extracellular proteins. Unlike other LTBPs, LTBP-2 does not covalently bind transforming growth factor-β1 (TGF-β1) but appears to be implicated in the regulation of TGF-β1 bioactivity, although the mechanisms ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18102114
更新日期：2017-10-09 00:00:00
