Abstract:
:Hetero-dimeric cytokines often require equi-molar expression of both subunits to achieve biological activity. Previously, we expressed ovine IL-12 p40 and p35 linked using a self-cleaving 2A peptide from foot-and-mouth disease virus (FMDV). We now generated a new improved vector for the expression of hetero-dimeric cytokines and demonstrate the more general applicability of this strategy by cloning and expressing ovine IL-23 using the 2A peptide to link IL-12/IL-23 p40 and p19. The resulting protein was shown to be biologically active when expressed in mammalian COS cells. IL-23 plays a significant role in the differentiation of Th17 cells as well as autoimmunity and the regulation of inflammatory processes. As such this reagents will be invaluable in the unravelling of regulation of the ovine immune system for both veterinary and human animal model applications.
journal_name
Cytokinejournal_title
Cytokineauthors
Yen HH,Scheerlinck JPdoi
10.1016/j.cyto.2013.01.014subject
Has Abstractpub_date
2013-03-01 00:00:00pages
744-6issue
3eissn
1043-4666issn
1096-0023pii
S1043-4666(13)00046-Xjournal_volume
61pub_type
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