Abstract:
:This moleculo-epidemiological and immunological study through cytokine response assessment was done to know the dynamics of cytokines in the initiation, persistence and association to physiological changes of a particular pathogen in water buffaloes. This is important to understand the magnitude and behavior of disease progression. Water buffalo blood samples gathered from different places in the Philippines revealed a 9.4%, 27.6%, 10.3% and 4.4% prevalence of bovine viral diarrhea virus (BVDV), bovine leukemia virus (BLV), Anaplasma marginale and Babesia bigemina infection, respectively. This was the first surveillance study of BVDV and BLV in the country. Furthermore, cytokine expression of these naturally infected animals was also quantified. BVDV-infected animals had up-regulated expressions of TNFalpha, IL-2 and IL-4; and down-regulated expressions of IFNgamma and IL-12p40 while BLV positive animals had an up-regulated IL-4 and IL-6, and highly expressed IL-10 and IL-12p40 with unchanged IFNgamma expression. Meanwhile, animals infected with A. marginale had all interleukins and IFNgamma up-regulated with significant expression of IL-10 and IL-12p40 similar to the BLV positive animals. Since it was also observed that swamp-type buffaloes were more disease tolerant than riverine-type buffaloes based on the gathered infection rate of each examined pathogen, further assessment was done focusing on the two vital cytokines, IFNgamma and TNFalpha. We quantified IFNgamma and TNFalpha expressions in ConA-stimulated PBMC from both swamp and riverine buffaloes by real-time PCR. Cytokine expression from ConA-stimulated PBMC revealed that both IFNgamma and TNFalpha were more highly expressed in swamp than in riverine buffalo. To further examine the probable cause of expression differences, the proximal promoter region of these two cytokines were sequenced for the presence of nucleotide polymorphism followed by luciferase assay to analyze the effect of these polymorphisms in gene transcription. A single nucleotide polymorphism was found in the IFNgamma (-299) while eight polymorphisms in the TNFalpha promoter (-541, -553, -562, -596, -609, -655, -659, -688). Luciferase assay showed that both IFNgamma promoter and TNFalpha promoter in swamp-type water buffalo had higher transcription activity compared to riverine-type water buffalo. These findings confirm that IFNgamma and TNFalpha transcriptions in these animals were highly affected by the disparity in the cytokine promoter region. This suggests that disease tolerance or susceptibility of these buffaloes could be due to the differences in their relative cytokine transcription and may relate to pathogen-host specific pathogenesis.
journal_name
Cytokinejournal_title
Cytokineauthors
Mingala CN,Konnai S,Cruz LC,Onuma M,Ohashi Kdoi
10.1016/j.cyto.2009.02.006subject
Has Abstractpub_date
2009-05-01 00:00:00pages
273-82issue
2eissn
1043-4666issn
1096-0023pii
S1043-4666(09)00060-Xjournal_volume
46pub_type
杂志文章相关文献
CYTOKINE文献大全abstract::The present study aims to characterize the role of the citrulline-nitric oxide cycle in the response of adult human and rat pancreatic islets to cytokines. Citrulline (0.1-1.0 mM) or arginine (0.1-1.0 mM) led to a similar dose dependent nitric oxide (NO) production by rat islets exposed to interleukin 1 beta (IL-1 bet...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1996.0086
更新日期:1996-08-01 00:00:00
abstract:BACKGROUND:The effectual immune response is crucial to defeat viral infections. However, exuberant immune response with features of macrophage activation syndrome (MAS) lead detrimental consequences in COVID-19 patients. Interleukin (IL)-18 is one of the leading cytokines in MAS which has not been studied in COVID-19. ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2020.155302
更新日期:2021-01-01 00:00:00
abstract:BACKGROUND:Vascular endothelial growth factor (VEGF) is the key regulator of angiogenesis in the development of various cancers. Previous studies have examined the relationship between VEGF gene promoter polymorphisms such as -2578C/A and -460C/T and bladder cancer risk; however, these results are inconclusive. Therefo...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2020.155112
更新日期:2020-07-01 00:00:00
abstract::Overweight and obesity are associated with chronic and subclinical inflammation due to an imbalance of inflammatory mediators. However, the association with gene polymorphism has been rarely studied in children. The aim of this study was to determine if serum concentrations of C-reactive protein (CRP) and interleukin-...
journal_title:Cytokine
pub_type: 临床试验,杂志文章
doi:10.1016/j.cyto.2016.09.007
更新日期:2016-12-01 00:00:00
abstract::Tumor necrosis factor-alpha (TNF) is expressed on the cell surface as a transmembrane form (tmTNF), that can be released as a soluble form (solTNF) via proteolytic cleavage. These two types of TNF exert their biological functions by binding to one of two TNF receptors, TNFR1 or TNFR2. However, the biological function ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2009.11.022
更新日期:2010-04-01 00:00:00
abstract::The transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) regulates a battery of antioxidant, detoxification, and cell stress genes. It is activated by oxidative stress and a number of exogenous compounds, one of which is tert-butylhydroquinone (tBHQ), a widely used food preservative. Nrf2 modulates ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2014.11.006
更新日期:2015-02-01 00:00:00
abstract:BACKGROUND:Alterations in β common (βC) and γ common (γC) chain cytokines have been described in pulmonary tuberculosis. However, their role in tuberculous lymphadenitis (TBL) disease has not been assessed. METHODS:Thus, in the present study, we have examined the systemic levels of βC and γC chain cytokines in TBL, la...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2020.155405
更新日期:2021-02-01 00:00:00
abstract::Dysregulation of cytokines synthesis is thought to play a role in the development of a number of age-related conditions, such as rheumatoid arthritis, osteoporosis, atherosclerosis, and others, but observational studies have led to contradictory results. We investigated potential familial influences on the plasma leve...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2002.1959
更新日期:2002-08-07 00:00:00
abstract::The production of nitric oxide (NO) is increased in experimental nephritis, with NO thought to be an important mediator of cell damage. The cytokines interleukin 1 beta (IL-1 beta), IL-6, IL-8, monocyte chemotactic protein-1 (MCP-1) and transforming growth factor-beta (TGF-beta) are released from mesangial cells in vi...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/1043-4666(94)90047-7
更新日期:1994-11-01 00:00:00
abstract:BACKGROUND:The genetic basis of complex diseases like ischemic stroke probably consists of several predisposing risk factors, such as genes involved in inflammation and thrombotic pathways. On this basis the aim of our study was to evaluate the role of SNPs (single nucleotide polymorphisms) of some pro-inflammatory/ant...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2012.02.012
更新日期:2012-06-01 00:00:00
abstract::The authors studied plasma cytokine activity using cross reactive polyclonal antibodies to human cytokines in young and old female monkeys to determine if age had any effect on cytokine secretion, and compared hormonal effects as well. While interleukin 1 (IL-1) was not higher in aged cycling monkeys, it was after men...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1997.0281
更新日期:1998-03-01 00:00:00
abstract::Emerging evidence indicates that chemokine receptor expression patterns are critical in determining the spectrum of action of the chemokines. We have analysed the expression patterns of 17 chemokine receptors and two orphan chemokine receptor-like genes in various freshly prepared human peripheral blood leucocyte popu...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2000.0851
更新日期:2001-04-07 00:00:00
abstract::Previous studies have suggested that heterotrimeric G(i) proteins, Src tyrosine kinase and phosphatidylinositol-3 kinase (PI3 Kinase) are involved in signaling events induced by lipopolysaccharide (LPS) leading to pro-inflammatory cytokines gene expression. To investigate the involvement of these mediators in Gram-pos...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/s1043-4666(03)00122-4
更新日期:2003-06-07 00:00:00
abstract::Transforming growth factor-beta1 (TGFbeta1) induces plasminogen activator inhibitor-1 (PAI-1) as a major target protein. PAI-1 is associated with fibrosis, thrombosis, and metabolic disorders. TGFbeta1 induces PAI-1 via phosphorylation and nuclear translocation of Smads. Oltipraz inhibits TGFbeta1 expression and also ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2006.10.001
更新日期:2006-09-01 00:00:00
abstract::IL-18 is a pleiotropic and multifunctional cytokine that belongs to the IL-1 family. It is produced as a biologically inactive precursor, which is cleaved into its active mature form mainly by caspase-1. The caspase becomes active from its inactive precursor (procaspase-1) upon assembly of an inflammasome. Because of ...
journal_title:Cytokine
pub_type: 杂志文章,评审
doi:10.1016/j.cyto.2016.01.006
更新日期:2016-06-01 00:00:00
abstract:UNLABELLED:Burn is associated with profound inflammation and activation of the innate immune system in multiple organ beds, including the lung. Similarly, toll-like receptors (TLR) are associated with innate immune activation. Nonetheless, it is unclear what impact burn has on TLR-induced inflammatory responses in the ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2011.05.004
更新日期:2011-09-01 00:00:00
abstract:BACKGROUND:Fibrosis is attributed to dysregulation of tissue-remodeling. In remodeling areas, fibroblasts and macrophages actively make contact with each other. Osteopontin (OPN) is a pro-fibrotic molecule, whose expression is upregulated by interleukin (IL)-1β via secretion of its downstream cytokines, such as IL-6. H...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2018.04.025
更新日期:2018-10-01 00:00:00
abstract::Preeclampsia is a complex disease of pregnancy with both feto-placental and maternal factors contributing to its pathogenesis. Although the cause of this disease is uncertain, imbalance between pro-and anti-inflammatory cytokines has been implicated in the pathogenesis of preeclampsia. Increased levels of the inflamma...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2012.07.039
更新日期:2012-12-01 00:00:00
abstract::The pathogenesis of septic shock is mainly due to unregulated tumour necrosis factor-alpha (TNF-alpha) production. Procalcitonin (PCT) and calcitonin gene-related peptide (CGRP) are alternative transcription products of the calcitonin gene. Since high PCT levels have been described in human sepsis, and since CGRP inhi...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1999.0607
更新日期:2000-06-01 00:00:00
abstract::This study investigated changes in the myokines IL-1β, IL-6, and TNF-α, as well as HSP72, after endurance training and after a session of downhill running. Twenty-eight rats were allocated to four different groups: 1. Eight weeks of endurance training at 65-70% VO2max (Trained); 2. Endurance training and a single sess...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2015.03.013
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVE:Functions of the Th9 cells and its signature cytokine IL-9 in human autoimmune diseases is currently under extensive research. Here we are reporting new functions of IL-9-receptor (IL-9R); its regulatory role on (i) FLS (fibroblast like synoviocyte) biology and (ii) pannus formation in rheumatoid arthritis (R...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2018.08.001
更新日期:2018-11-01 00:00:00
abstract::CCR2, and its principle ligand MCP-1/CCL2, have been well documented for their ability to induce monocyte infiltration and promote the pathogenesis of rheumatoid arthritis and atherosclerosis. In order to assess additional roles for CCR2, we inserted allogeneic implants into CCR2-/- and MCP-1-/- mice and characterized...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2002.1031
更新日期:2002-05-21 00:00:00
abstract::Thymic vulnerability, a leading cause of defective immunity, was discovered decades ago. To date, several strategies have been investigated to unveil any immunorestorative capacities they might confer. Studies exploiting castration, transplantation, adoptive cell therapies, hormones/growth factors, and cytokines have ...
journal_title:Cytokine
pub_type: 杂志文章,评审
doi:10.1016/j.cyto.2019.05.009
更新日期:2019-08-01 00:00:00
abstract::In hypertension studies, anti-inflammatory cytokine interleukin-10 (IL-10) has been shown to prevent angiotensin II (Ang II)-induced vasoconstriction and regulate vascular function by down-regulating pro-inflammatory cytokine and superoxide production in vascular cells. However, little is known about the mechanism beh...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2015.09.002
更新日期:2016-01-01 00:00:00
abstract::Granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates production of neutrophils in bone marrow and may decrease the incidence of infection during neutropenia. We evaluated the protective role of recombinant GM-CSF against Pseudomonas aeruginosa challenge in neutropenic mice. CD-1 mice treated with cyclo...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/1043-4666(90)90030-w
更新日期:1990-07-01 00:00:00
abstract::Cytotoxic action of leukocytes may be involved in the pathogenesis of inflammatory heart muscle disorders. We investigated the expression of rat leukocyte chemotactic cytokines--cytokine induced neutrophil chemoattractant (CINC) and JE--in cultured neonatal rat cardiac myocytes; CINC belongs to the interleukin 8 (IL-8...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1995.0037
更新日期:1995-04-01 00:00:00
abstract::The aim of the study was to characterize and to quantify peripheral and tissue. IL-35- and IL-37-producing cells in Inflammatory Bowel Disease (IBD) patients. We studied a total of 38 active UC, 31 inactive UC, 17 active CD, and 13 inactive CD and 50 non-inflamed tissues as control group. Gene expression was measured ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2015.04.009
更新日期:2015-10-01 00:00:00
abstract::Neutrophil-activating peptide 1/interleukin 8 (NAP-1/IL-8) is a recently described cytokine with potent chemotactic activity for human neutrophil granulocytes (PMN) and T cells. In psoriasis, a chronic hyperproliferative and inflammatory skin disorder, PMN and T cells are found as prominent cells in the inflammatory i...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/1043-4666(91)90500-d
更新日期:1991-07-01 00:00:00
abstract::Recently, a novel technique for "real time" quantitative Reverse Transcriptase-PCR which measures PCR-product accumulation during the exponential phase of the PCR reaction using a dual-labelled fluorogenic probe, has been developed. This method allows direct detection of PCR-product formation by measuring the increase...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1998.0426
更新日期:1999-04-01 00:00:00
abstract::Adiponectin, the most prevalent adipo-cytokine in plasma plays critical metabolic and anti-inflammatory roles is fast emerging as an important molecular target for the treatment of metabolic disorders. Adiponectin action is critical in multiple organs including cardio-vascular system, muscle, liver, adipose tissue, br...
journal_title:Cytokine
pub_type: 杂志文章,评审
doi:10.1016/j.cyto.2018.06.012
更新日期:2018-12-01 00:00:00