Abstract:
:Span 80 (sorbitan monooleate) vesicles behaved differently from conventional phospholipid vesicles (liposomes) because the former had a more fluid interface. After doxorubicin hydrochloride (DOX) was encapsulated into the Span 80 vesicle (loading efficiency: 63 %), DOX-loaded Span 80 vesicles (DVs) were thereafter added to Colon 26 cells. It was suggested, from the flow cytometric analysis and confocal laser microscopic observation, that DVs directly deliver DOX into the cytoplasm of Colon 26 cells. DVs showed the different delivery manner from the DOX-loaded liposomes (DLs). It is considered that the difference of delivery manner between DVs and DLs resulted in the difference of cytotoxicity (IC(50)); i.e. IC(50) values for DVs and DLs were 5 and > 30 μM, respectively. The results obtained herein would give the fundamental findings which can contribute to the improvement of formulation of conventional liposome-based carrier and its cytotoxicity.
journal_name
Int J Biol Scijournal_title
International journal of biological sciencesauthors
Hayashi K,Tatsui T,Shimanouchi T,Umakoshi Hdoi
10.7150/ijbs.5453subject
Has Abstractpub_date
2013-01-01 00:00:00pages
142-8issue
2issn
1449-2288pii
ijbsv09p0142journal_volume
9pub_type
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