当前位置： SCI文献检索 > ATHEROSCLEROSIS期刊下所有文献 > A comparison of the accuracy of Illumina HumanHT-12 v3 Expression BeadChip and TaqMan qRT-PCR gene expression results in patient samples from the Tampere Vascular Study.

A comparison of the accuracy of Illumina HumanHT-12 v3 Expression BeadChip and TaqMan qRT-PCR gene expression results in patient samples from the Tampere Vascular Study.

Abstract：

AIMS:This study was set up to compare the accuracy, sensitivity and specificity of gene expression results between the Illumina HumanHT-12 v3 Expression BeadChip (GWE) and the TaqMan qRT-PCR low-density array (LDA) in atherosclerotic plaques. METHODS:Gene expression levels of 196 genes were determined from 22 atherosclerotic samples and 6 controls with both the GWE and the LDA. RESULTS:The accuracies of GWE in comparison to that of qRT-PCR for absolute fold changes (FC) 1.2, 1.6, 2.0 and 3.0 between cases and controls were 73.5%, 79.1%, 72.4% and 60.7%, respectively. The correlation of expression measurements between these methods was good (r = 0.87, y = 0.151 + 0.586x), and the Bland-Altman plot showed that for highly up- or down-regulated transcripts, GWE yields lower absolute FC values than LDA. CONCLUSION:Gene expression results obtained with the GWE are replicated with high accuracy in LDA, even for technically demanding atherosclerotic samples.

journal_name

Atherosclerosis

journal_title

Atherosclerosis

authors

Raitoharju E,Seppälä I,Lyytikäinen LP,Levula M,Oksala N,Klopp N,Illig T,Laaksonen R,Kähönen M,Lehtimäki T

doi

10.1016/j.atherosclerosis.2012.10.078

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

149-52

issue

1

eissn

0021-9150

issn

1879-1484

pii

S0021-9150(12)00795-2

journal_volume

226

pub_type

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