Abstract:
AIMS:The ubiquitin-activating enzyme E1 (UBA1, E1), the apex of the ubiquitin proteasome pathway, plays a critical role in protein degradation and in pathological processes. Whether UBA1 participates the development of vascular restenosis remains unknown. This study aims to determine the role of UBA1 in the development of balloon injury induced neointimal formation. METHODS AND RESULTS:Immunostaining and western blots were used to examine the expression of the ubiquitinated protein in the injured carotid after angioplasty. Higher levels of ubiquitinated protein were observed in the neointima. Local delivery of potent chemical UBA1 inhibitor PYR-41 (100 μM) and UBA1 shRNA lentivirus both resulted in a substantial decrease in intimal hyperplasia at 2 weeks and 4 weeks after balloon injury. UBA1 inhibition also reduced Ki-67 positive cell percentage and inflammatory response in the carotid artery wall. We further determined that in vitro UBA1 inhibition was able to ameliorate TNF-α-induced nuclear factor-kappa B (NF-κB) activation by reducing IκB degradation in vascular smooth muscle cells (VSMCs). UBA1 inhibition also led to the accumulation of short-lived proteins such as p53, p21 and c-jun, which may account for the UBA1 inhibition-induced cell cycle delay. Thus, VSMCs proliferation was blocked. CONCLUSIONS:UBA1 inhibition effectively suppresses neointimal thickening through its anti-proliferative and anti-inflammatory effects. Our results provide further evidence that the ubiquitin-proteasome system is a potential new target for the prevention of vascular restenosis.
journal_name
Atherosclerosisjournal_title
Atherosclerosisauthors
Qin Z,Cui B,Jin J,Song M,Zhou B,Guo H,Qian D,He Y,Huang Ldoi
10.1016/j.atherosclerosis.2016.02.016subject
Has Abstractpub_date
2016-04-01 00:00:00pages
142-53eissn
0021-9150issn
1879-1484pii
S0021-9150(16)30059-4journal_volume
247pub_type
杂志文章相关文献
ATHEROSCLEROSIS文献大全abstract::The hypolipidemic response of rice bran oil (RBO) was investigated in nonhuman primates fed semi-purified diets containing blends of oils which included RBO at 0-35% Kcals as dietary fat. The studies demonstrated the following: (a) the degree of reduction of serum total cholesterol (TC) and low density lipoprotein cho...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(91)90075-e
更新日期:1991-06-01 00:00:00
abstract::The composition of very low density lipoproteins (VLDL: d less than 1.019) of New Zealand male rabbits reciving cholesterol (2 g/day) and metformin (135 mg/kg/day) is investigated. These rabbits, while showing only a slight reduction of plasma cholesterol levels, as compared to cholesterol-fed (h.c.) animals, show a m...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(77)90142-3
更新日期:1977-01-01 00:00:00
abstract::To determine whether a relationship between echographically assessed carotid artery and left ventricular (LV) structures existed in asymptomatic men at risk for cardiovascular disease, we evaluated carotid and LV parameters in 69 subjects (23-62 years) without LV hypertrophy. The right common carotid far wall intima-m...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(96)05940-0
更新日期:1996-11-15 00:00:00
abstract::The acidic glycosaminoglycans (AGAG) of the human coronary arterial tree (the main left and right branches and their distal portions) were analyzed by enzymatic methods employing chondroitinases, hyaluronidase and heparitinase. The AGAG content of human coronary arteries was highest in the left branch, intermediate in...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(82)90171-x
更新日期:1982-10-01 00:00:00
abstract:OBJECTIVE:The objective of this article is to review the current literature on Wnt5a and its signaling mechanism, along with its role in atherosclerosis. In addition, the significance of Wnt5a as a diagnostic marker and a potential therapeutic target is reviewed. Wnt5a, a secreted glycoprotein, belongs to a family of h...
journal_title:Atherosclerosis
pub_type: 杂志文章,评审
doi:10.1016/j.atherosclerosis.2014.08.027
更新日期:2014-11-01 00:00:00
abstract::Familial hypobetalipoproteinemia is an autosomal co-dominant disorder, which in a minority of cases is due to a truncation producing mutation in the apoB gene. We have identified an apoB mutation in a 40-year old hypobetalipoproteinemic man with Type II diabetes mellitus. Immunoblotting of plasma revealed a major band...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(97)00222-0
更新日期:1998-02-01 00:00:00
abstract::We studied the aortic histology of 28 Macaca nigra males and females, from 6 to more than 20 years old, normal and manifesting various degrees of spontaneous diabetes. Correlations of several metabolic and hormonal indicators of diabetes severity with gross and microscopic findings in the aortas demonstrated direct as...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(84)90158-8
更新日期:1984-07-01 00:00:00
abstract:OBJECTIVE:Antiphospholipid antibodies are associated with increased risk of thrombosis, particularly as in antiphospholipid syndrome. This study aims to determine the acute effects of anticardiolipin antibodies on nitric oxide production and vascular function. METHODS:Ex vivo aortic rings from male Sprague Dawley rats...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2005.06.044
更新日期:2006-04-01 00:00:00
abstract:OBJECTIVE:Associations of glycated hemoglobin A1c (HbA1c) levels to incident coronary and cardiovascular events among non-diabetic patients with coronary artery disease are unclear. We investigated relations of HbA1c to long-term prognosis in such patients. METHODS:A prospective cohort of 2519 patients undergoing elec...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2015.02.053
更新日期:2015-05-01 00:00:00
abstract::We previously demonstrated that hyperbaric oxygen (HBO) treatment inhibits diet-induced atherosclerosis in New Zealand White rabbits. In the present study we investigate the mechanisms that might be involved in the athero-protective effect of HBO treatment in a well-accepted model of atherosclerosis, the apoE knockout...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2006.08.018
更新日期:2007-07-01 00:00:00
abstract::Low-density lipoprotein cholesterol (LDL-C) and the small dense LDL (SdLDL) phenotype are both predictors for ischemic heart disease. We examined whether cholesterol of SdLDL (SdLDL-C) is more closely associated with carotid artery intima-media thickness (CA-IMT), a surrogate measure of atherosclerosis, than LDL-C and...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2008.04.042
更新日期:2009-02-01 00:00:00
abstract::Blood serum of most patients with coronary heart disease (CHD) caused a 2-5-fold increase in the total cholesterol content of smooth muscle cells cultured from unaffected human aortic intima, i.e. possessed an atherogenic potential manifested in culture. Removal of immunoglobulins G and M from an atherogenic serum bro...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(90)90065-q
更新日期:1990-04-01 00:00:00
abstract::Young pigs with hypertension of 10 weeks duration, resulting from cellophane perinephritis, were injected with 125I-labelled low-density lipoprotein ([125I]LDL) before being killed 24 h or 48 h later. Intimal thickening and increased acid mucopolysaccharide were demonstrated in the aortas and major arteries of the hyp...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(80)90180-x
更新日期:1980-04-01 00:00:00
abstract::Factor XIII is a transglutaminase that crosslinks fibrin in the last steps of the coagulation process. A few polymorphic sites have been identified in this gene, one of them being a point mutation (FXIII Val34Leu), leading to an amino acid change of valine to leucine. Recently, in British patients, FXIII 34Leu allele ...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(98)00241-x
更新日期:1999-02-01 00:00:00
abstract::We describe a rapid screening procedure to identify known DNA sequence changes in individuals diagnosed as having heterozygous familial hypercholesterolaemia (FH). The screening is made possible by combining a rapid DNA extraction protocol and small scale polymerase chain reaction DNA amplification, followed by oligon...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(91)90053-6
更新日期:1991-08-01 00:00:00
abstract:BACKGROUND:Smoking affects the arterial wall and increases the risk of cardiovascular disease. It also affects the extracellular matrix in skin, causing impaired wound healing. However, little is known about putative molecular changes in the arterial wall. Our aim was to investigate the possible correlation between ext...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2016.02.022
更新日期:2016-04-01 00:00:00
abstract::The effect of the apolipoprotein C-II/C-III1 ratio on the capacity of purified bovine milk lipoprotein lipase to hydrolyse triglycerides was measured in a controlled model of pyrene-labeled nonanoyltriglycerides (1-2 ditetradecyl 3-pyrene nonanoyl glyceride) monolayer vesicles. Monolayer was composed of triglycerides,...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(96)05955-2
更新日期:1996-12-20 00:00:00
abstract:BACKGROUND:CD36 signal transductions have been reported by a variety of lipid moiety of oxidized low-density lipoprotein (oxLDL), however, CD36 signal induced by 7-ketocholesteryl-9-carboxynonanoate (oxLig-1), a lipid moiety of oxLDL has not been elucidated. METHODS AND RESULTS:OxLig-1 leads to activation and recruitm...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2012.10.038
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:In addition to being a risk marker for cardiovascular disease, recent data suggests that C-reactive protein (CRP) induces endothelial dysfunction and promotes oxidative stress. We evaluated the effects of two conformers of CRP (pentameric, or native [nCRP], versus monomeric, or modified [mCRP]) on vessel func...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2007.06.013
更新日期:2007-12-01 00:00:00
abstract:OBJECTIVE:The distribution of atherosclerotic lesions changes with age in human and rabbit aortas. We investigated if this can be explained by changes in patterns of blood flow and wall shear stress. METHODS:The luminal geometry of thoracic aortas from immature and mature rabbits was obtained by micro-CT of vascular c...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2012.04.020
更新日期:2012-07-01 00:00:00
abstract::Atherosclerosis is a complex disease that gene and environment interaction influences the progression of atherosclerotic lesion development. Our laboratory used mice lacking both the low density lipoprotein (LDL) receptor and Apobec1 genes (LDLR-/-Apobec1-/-, designated LDb) to investigate gene-gene interaction and th...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(03)00133-3
更新日期:2003-07-01 00:00:00
abstract::Rabbits were fed cholesterol for 14 weeks to study the effect of probucol on atheroma formation. Three groups of animals were investigated: group CHOL was fed 1% cholesterol and served as control for group P + CHOL. fed 1% cholesterol and 1% probucol from the onset till the end of the experiment: group CHOL + P receiv...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(89)90171-8
更新日期:1989-02-01 00:00:00
abstract::The metabolism of homocysteine requires contributions of several enzymes and vitamin cofactors. Earlier studies identified a common polymorphism of methylenetetrahydrofolate reductase that was associated with mild hyperhomocysteinemia. Common variants of two other enzymes involved in homocysteine metabolism, methionin...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(02)00204-6
更新日期:2003-01-01 00:00:00
abstract:AIMS:Transition metal ions have been implicated in atherosclerosis. The goal of this study was to investigate whether metal ion levels were higher in people with diabetes, in view of their increased risk of aggravated atherosclerosis. METHODS AND RESULTS:Absolute concentrations of iron, copper, zinc and calcium, and p...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2012.03.015
更新日期:2012-06-01 00:00:00
abstract::Increased atherosclerosis risk in hyperlipidemic patients may be a result of the enhanced oxidizability of their plasma lipoproteins. We have previously shown that hypocholesterolemic drug therapy, including the 3-hydroxy-3-methyl-glutaryl CoenzymeA (HMG-CoA) reductase inhibitors, and the hypotriglyceridemic drug beza...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(98)00032-x
更新日期:1998-06-01 00:00:00
abstract::It has been shown that triglyceride levels are one of the determinants of factor VII levels. In this study we have simultaneously evaluated, in a group of 102 healthy individuals, the different forms of factor VII, namely factor VII mass, factor VII coagulant activity, activated factor VII double-chain form and factor...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(93)90050-5
更新日期:1993-02-01 00:00:00
abstract::Endurance exercise training is known to produce favorable changes in the metabolic profile including reduced plasma triglyceride (TG) and increased high-density lipoprotein (HDL) cholesterol concentrations. These metabolic improvements are likely to contribute to the reduced coronary heart disease (CHD) risk often obs...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(01)00515-9
更新日期:2001-12-01 00:00:00
abstract:PURPOSE:Metabolic Syndrome as defined by ATP III criteria, a constellation of risk factors associated with insulin resistance, predisposes to premature atherosclerosis and early coronary events. Whether that negative risk profile is associated with endothelial dysfunction remains to be established. MATERIALS AND METHO...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2003.11.013
更新日期:2004-02-01 00:00:00
abstract::This experiment involved examination of the effects of gender and social status ('competitive dominance') on the coronary artery atherosclerosis of cynomolgus monkeys. Thirty-two adult Macaca fascicularis (16 males, 16 females) were fed a diet containing a moderate amount of cholesterol (0.56 mg/cal) for 16 months. Th...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(83)90040-0
更新日期:1983-09-01 00:00:00
abstract::Short-term exercise training has been associated with improved endothelial-dependent vasodilation, but the impact of long-term habitual physical activity on vascular reactivity is not established. We studied the correlation between self-reported, habitual physical activity and vasoreactivity in non-smoking, non-diabet...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(01)00529-9
更新日期:2001-12-01 00:00:00