Abstract:
:Cell mechanical microenvironment (CMM) significantly affects cell behaviors such as spreading, migration, proliferation and differentiation. However, most studies on cell response to mechanical stimulation are based on two-dimensional (2D) planar substrates, which cannot mimic native three-dimensional (3D) CMM. Accumulating evidence has shown that there is a significant difference in cell behavior in 2D and 3D microenvironments. Among the materials used for engineering 3D CMM, hydrogels have gained increasing attention due to their tunable properties (e.g. chemical and mechanical properties). In this paper, we provide an overview of recent advances in engineering hydrogel-based 3D CMM. Effects of mechanical cues (e.g. hydrogel stiffness and externally induced stress/strain in hydrogels) on cell behaviors are described. A variety of approaches to load mechanical stimuli in 3D hydrogel-based constructs are also discussed.
journal_name
Biofabricationjournal_title
Biofabricationauthors
Huang G,Wang L,Wang S,Han Y,Wu J,Zhang Q,Xu F,Lu TJdoi
10.1088/1758-5082/4/4/042001subject
Has Abstractpub_date
2012-12-01 00:00:00pages
042001issue
4eissn
1758-5082issn
1758-5090journal_volume
4pub_type
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