Abstract:
:A recently emerged approach for tissue engineering is to biofabricate tissues using cellular spheroids as building blocks. Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), can be cultured to generate large numbers of cells and can presumably be differentiated into all the cell types of the human body in vitro, thus are an ideal cell source for biofabrication. We previously developed a hydrogel-based cell culture system that can economically produce large numbers of hPSC spheroids. With hPSCs and this culture system, there are two potential methods to biofabricate a desired tissue. In Method 1, hPSC spheroids are first utilized to biofabricate an hPSC tissue that is subsequently differentiated into the desired tissue. In Method 2, hPSC spheroids are first converted into tissue spheroids in the hydrogel-based culture system and the tissue spheroids are then utilized to biofabricate the desired tissue. In this paper, we systematically measured the fusion rates of hPSC spheroids without and with differentiation toward cortical and midbrain dopaminergic neurons and found spheroids' fusion rates dropped sharply as differentiation progressed. We found Method 1 was appropriate for biofabricating neural tissues.
journal_name
Biofabricationjournal_title
Biofabricationauthors
Lin H,Li Q,Lei Ydoi
10.1088/1758-5090/aa663bsubject
Has Abstractpub_date
2017-04-24 00:00:00pages
025007issue
2eissn
1758-5082issn
1758-5090journal_volume
9pub_type
杂志文章相关文献
Biofabrication文献大全abstract::Human induced pluripotent stem cells (hiPSCs) can be differentiated at high efficiency into cells of a targeting type but the resulting cell population has to be of high purity for clinical therapies to avoid teratomas. Herein, we report a microfluidic device with integrated and surface functionalised fishnet-like str...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/8/3/035017
更新日期:2016-09-08 00:00:00
abstract::Laser-assisted bioprinting (LaBP) allows the realization of computer-generated 3D tissue grafts consisting of cells embedded in a hydrogel environment. In this study, human adipose-derived stem cells (hASCs) were printed in a free-scalable 3D grid pattern by means of LaBP. We demonstrate that neither the proliferation...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/3/1/015005
更新日期:2011-03-01 00:00:00
abstract::Advances in three-dimensional cell cultures offer new opportunities in biomedical research and drug development. However, there are still challenges to overcome, including the lack of reliability, repeatability and complexity of tissues obtained by these techniques. In this study, we describe a new bioprinting system ...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aaf625
更新日期:2018-12-27 00:00:00
abstract::Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated. Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers whic...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab860d
更新日期:2020-05-12 00:00:00
abstract::Technical limitations of traditional electrospinning make it hard to produce three-dimensional (3D) scaffolds with hierarchical pore structures. Here, porous polycaprolactone (PCL) nanofiber meshes with different nano-hydroxyapatite (nHA) concentrations were prepared by electrospinning with stainless steel mesh as the...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa5c99
更新日期:2017-02-14 00:00:00
abstract::An ordered cellulose film scaffold, termed a nematic ordered cellulose (NOC) template, had unique surface properties and successfully induced the establishment of a three-dimensional (3D), hierarchical structure of epidermal cells by cell attachment and subsequent culture. Initially, the scaffold surface properties we...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/5/2/025010
更新日期:2013-06-01 00:00:00
abstract::Calcium alginate hydrogels are widely used as biocompatible materials in a substantial number of biomedical applications. This paper reports on a hybrid 3D printing and electrodeposition approach for forming 3D calcium alginate hydrogels in a controllable manner. Firstly, a specific 3D hydrogel printing system is deve...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa6ed8
更新日期:2017-06-07 00:00:00
abstract::Bone healing is regulated by multiple microenvironmental signals provided by the extracellular matrix (ECM). This study aimed to mimic the native osteoinductive microenvironment by developing an ECM using gene-transduced cells. The LIM mineralization protein-1 (LMP-1) gene was transferred to murine pre-osteoblast cell...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa8dd1
更新日期:2017-11-14 00:00:00
abstract::Tissue engineering aims to create implantable biomaterials for the repair and regeneration of damaged tissues. In vitro tissue engineering is generally based on static culture, which limits access to nutrients and lacks mechanical signaling. Using shear stress is controversial because in some cases it can lead to cell...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aba412
更新日期:2020-08-10 00:00:00
abstract::Four-dimensional (4D) bioprinting, encompassing a wide range of disciplines including bioengineering, materials science, chemistry, and computer sciences, is emerging as the next-generation biofabrication technology. By utilizing stimuli-responsive materials and advanced three-dimensional (3D) bioprinting strategies, ...
journal_title:Biofabrication
pub_type: 杂志文章,评审
doi:10.1088/1758-5090/9/1/012001
更新日期:2016-12-02 00:00:00
abstract::We demonstrate a simple, accurate and versatile method to manipulate Parylene C, a material widely known for its high biocompatibility, and transform it to a substrate that can effectively control the cellular microenvironment and consequently affect the morphology and function of the cells in vitro. The Parylene C sc...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/2/025004
更新日期:2014-06-01 00:00:00
abstract::Scaffold-free 3D cell cultures (e.g. pellet cultures) are widely used in medical science, including cartilage regeneration. Their drawbacks are high time/reagent consumption and lack of early readout parameters. While optimisation was achieved by automation or simplified spheroid generation, most culture systems remai...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/abe186
更新日期:2021-01-29 00:00:00
abstract::We have developed cell sheet technology as a modern method for the fabrication of functional tissue-like and organ-like structures. This technology allows for a sheet of interconnected cells and cells in full contact with their natural extracellular environment to be obtained. A cell sheet can be patterned and compose...
journal_title:Biofabrication
pub_type: 杂志文章,评审
doi:10.1088/1758-5082/1/2/022002
更新日期:2009-06-01 00:00:00
abstract::Critical understanding of the complex metastatic cascade of prostate cancer is necessary for the development of a therapeutic interventions for treating metastatic prostate cancer. Increasing evidence supports the synergistic role of biochemical and biophysical cues in cancer progression at metastases. The biochemical...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/abd9d6
更新日期:2021-01-08 00:00:00
abstract::In recent years, novel biofabrication technologies have enabled the rapid manufacture of hydrogel-cell suspensions into tissue-imitating constructs. The development of novel materials for biofabrication still remains a challenge due to a gap between contradicting requirements such as three-dimensional printability and...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/8/2/025011
更新日期:2016-05-20 00:00:00
abstract::We previously developed the Bio-Pick, Place, and Perfuse (Bio-P3) instrument to fabricate large perfusable tissue constructs by stacking and aligning scaffold-free living microtissues with integrated lumens. The Bio-P3 required an actuating mechanism to manipulate living microtissues of various sizes and shapes that a...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/8/2/025015
更新日期:2016-05-25 00:00:00
abstract::Cell cultures for tissue engineering are traditionally prepared on two-dimensional or three-dimensional scaffolds with simple pores; however, this limits mass transportation, which is necessary for cell viability and function. In this paper, an innovative method is proposed for fabricating porous scaffolds with design...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/4/1/015004
更新日期:2012-03-01 00:00:00
abstract::Cell-imprinting technology is a novel method for directing stem cell fate using substrates molded from target cells. Here, we fabricated and studied cell-imprinted substrates for neural priming in human adipose-derived stem cells in the absence of chemical cues. We molded polydimethylsiloxane (PDMS) silicone substrate...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/abc66f
更新日期:2020-10-30 00:00:00
abstract::The high attrition rate of neuro-pharmaceuticals as they proceed to market necessitates the development of clinically-relevant in vitro neural microphysiological systems that can be utilized during the preclinical screening phase to assess the safety and efficacy of potential compounds. Historically, proposed models h...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab41b4
更新日期:2019-10-21 00:00:00
abstract::Plasma treatment is a method to modify the physicochemical properties of biomaterials, which consequently may affect interactions with cells. Based on the rationale that physical cues on the surface of culture substrates and implants, such as surface roughness, have proven to alter cell behaviour, we used electrospinn...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/5/1/015006
更新日期:2013-03-01 00:00:00
abstract::Nowadays, 3D bioprinting technologies are rapidly emerging in the field of tissue engineering and regenerative medicine as effective tools enabling the fabrication of advanced tissue constructs that can recapitulate in vitro organ/tissue functions. Selecting the best strategy for bioink deposition is often challenging...
journal_title:Biofabrication
pub_type: 杂志文章,评审
doi:10.1088/1758-5090/aae605
更新日期:2018-11-09 00:00:00
abstract::Tissue engineering has been developed with the ultimate aim of manufacturing human organs, but success has been limited to only thin tissues and tissues with no significant structures. In order to construct more complicated tissues, we have developed a three-dimensional (3D) fabrication technology in which 3D structur...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/3/3/034113
更新日期:2011-09-01 00:00:00
abstract::Fabricating three-dimensional, biocompatible microenvironments to support functional tissue assembly remains a key challenge in cardiac tissue engineering. We hypothesized that since the omentum can be removed from patients by minimally invasive procedures, the obtained underlying matrices can be manipulated to serve ...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/2/024101
更新日期:2014-06-01 00:00:00
abstract::Cell therapy represents a promising option for revascularization of ischemic tissues. However, injection of dispersed cells is not optimal to ensure precise homing into the recipient's vasculature. Implantation of cell-engineered scaffolds around the occluded artery may obviate these limitations. Here, we employed the...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/8/1/015020
更新日期:2016-03-24 00:00:00
abstract::Bioprinting of living cells is rapidly developing as an advanced biofabrication approach to engineer tissues. Bioinks can be extruded in three-dimensions (3D) to fabricate complex and hierarchical constructs for implantation. However, a lack of functionality can often be attributed to poor bioink properties. Indeed, a...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab19fd
更新日期:2019-06-12 00:00:00
abstract::In conventional primary cultures, cardiac cells prepared from a newborn rat undergo spontaneous formation of cell clusters after several days. These cell clusters may be non-homogeneously distributed on a flat surface and show irregular beating which can be recorded by calcium ion imaging. In order to improve the cell...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/5/3/035013
更新日期:2013-09-01 00:00:00
abstract::Traditional drug discovery involves the screening of lead compounds from a chemical library by using cell-based high throughput screening (HTS) procedures. This has created a demand for the development of cell-based microarray chips for HTS of compounds. Although several cell-based microarray devices and procedures fo...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/9/1/011001
更新日期:2016-12-05 00:00:00
abstract::Electrospun fibers of natural polymers are desirable for biomedical applications such as tissue engineering. Crosslinking of electrospun fibers of natural polymers is needed to prevent dissolution in water and to enhance mechanical strength. In this study, an in situ UV-crosslinking method was developed for crosslinki...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/5/3/035008
更新日期:2013-09-01 00:00:00
abstract::In the pharmaceutical industry, new drugs are tested to find appropriate compounds for therapeutic purposes for contemporary diseases. Unfortunately, novel compounds emerge at expensive prices and current target evaluation processes have limited throughput, thus leading to an increase of cost and time for drug develop...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/4/3/035001
更新日期:2012-09-01 00:00:00
abstract::Cardiovascular diseases (CVDs) are considered the principal cause of worldwide death, being atherosclerosis the main etiology. Up to now, the predominant treatment for CVDs has been bypass surgery from autologous source. However, due to previous harvest or the type of disease, this is not always an option. For this re...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/abbd27
更新日期:2020-09-30 00:00:00