Abstract:
:We have developed cell sheet technology as a modern method for the fabrication of functional tissue-like and organ-like structures. This technology allows for a sheet of interconnected cells and cells in full contact with their natural extracellular environment to be obtained. A cell sheet can be patterned and composed according to more than one cell type. The key technology of cell sheet engineering is that a fabricated cell sheet can be harvested and transplanted utilizing temperature-responsive surfaces. In this review, we summarize different aspects of cell sheet engineering and provide a survey of the application of cell sheets as a suitable material for biofabrication and clinics. Moreover, since cell micropatterning is a key tool for cell sheet engineering, in this review we focus on the introduction of our approaches to cell micropatterning and cell co-culture to the principles of automation and how they can be subjected to easy robotics programming. Finally, efforts towards making cell sheet technology suitable for biofabrication and robotic biofabrication are also summarized.
journal_name
Biofabricationjournal_title
Biofabricationauthors
Hannachi IE,Yamato M,Okano Tdoi
10.1088/1758-5082/1/2/022002subject
Has Abstractpub_date
2009-06-01 00:00:00pages
022002issue
2eissn
1758-5082issn
1758-5090pii
S1758-5082(09)03347-8journal_volume
1pub_type
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