Abstract：
BACKGROUND:The extracellular matrix (ECM) provides a supportive microenvironment for cells, which is suitable as a tissue engineering scaffold. Mechanical stimulus plays a significant role in the fate of osteoblast, suggesting that it regulates ECM formation. Therefore, we investigated the influence of mechanical stimulus on ECM formation and bioactivity. METHODS:Mouse osteoblastic MC3T3-E1 cells were cultured in cell culture dishes and stimulated with mechanical tensile strain. After removing the cells, the ECMs coated on dishes were prepared. The ECM protein and calcium were assayed and MC3T3-E1 cells were re-seeded on the ECM-coated dishes to assess osteoinductive potential of the ECM. RESULTS:The cyclic tensile strain increased collagen, bone morphogenetic protein 2 (BMP-2), BMP-4, and calcium levels in the ECM. Compared with the ECM produced by unstrained osteoblasts, those of mechanically stimulated osteoblasts promoted alkaline phosphatase activity, elevated BMP-2 and osteopontin levels and mRNA levels of runt-related transcriptional factor 2 (Runx2) and osteocalcin (OCN), and increased secreted calcium of the re-seeded MC3T3-E1 cells. CONCLUSION:Mechanical strain promoted ECM production of osteoblasts in vitro, increased BMP-2/4 levels, and improved osteoinductive potential of the ECM. This study provided a novel method to enhance bioactivity of bone ECM in vitro via mechanical strain to osteoblasts.
journal_name
Biomed Eng Onlinejournal_title
Biomedical engineering onlineauthors
Guo Y,Zhang CQ,Zeng QC,Li RX,Liu L,Hao QX,Shi CH,Zhang XZ,Yan YXdoi
10.1186/1475-925X-11-80subject
Has Abstractpub_date
2012-10-25 00:00:00pages
80issn
1475-925Xpii
1475-925X-11-80journal_volume
11pub_type
杂志文章abstract：BACKGROUND:Reach-grasp movements are motor components commonly affected after stroke and directly related to the independence of these individuals. Evaluations of these activities can be performed using clinical instruments and assessed by detailed and costly kinematic analyses. The aim of this study was to develop an ...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-020-0758-7
更新日期：2020-03-04 00:00:00
abstract：PURPOSE:Coronary artery bypass graft (CABG) surgery represents the standard treatment of advanced coronary artery disease. Two major types of anastomosis exist to connect the graft to the coronary artery, i.e., by using an end-to-side or a side-to-side anastomosis. There is still controversy because of the differences ...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-6-35
更新日期：2007-09-26 00:00:00
abstract：BACKGROUND:There is a high probability of accidental perforation of the vertebral pedicle wall in pedicle screw insertion surgery. A pedicle screw (PS) measurement device with an optical probe has been reported to send out a warning signal before the PS tip breaking the vertebral pedicle wall. METHODS:In this study, w...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-13-148
更新日期：2014-11-01 00:00:00
abstract：BACKGROUND:Image quality assessment method of photoacoustic tomography has not been completely standardized yet. Due to the combined nature of photonic signal generation and ultrasonic signal transmission in biological tissue, neither optical nor ultrasonic traditional methods can be used without modification. An optic...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-11-37
更新日期：2012-07-13 00:00:00
abstract：BACKGROUND:Use of standardized and scientifically sound outcome measures is encouraged in clinical practice and research. With the development of newer rehabilitation therapies, we need technology-supported upper extremity outcome measures that are easily accessible, reliable and valid. 3-Dimensional printing (3D-print...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-020-00839-3
更新日期：2021-01-05 00:00:00
abstract：BACKGROUND:Pre-operative imaging devices generate high-resolution images but intra-operative imaging devices generate low-resolution images. To use high-resolution pre-operative images during surgery, they must be deformed to reflect intra-operative geometry of brain. METHODS:We employ biomechanical models, guided by ...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-9-51
更新日期：2010-09-15 00:00:00
abstract：BACKGROUND:In the last years, several methods and devices have been proposed to record the human mandibular movements, since they provide quantitative parameters that support the diagnosis and treatment of temporomandibular disorders. The techniques currently employed suffer from a number of drawbacks including high pr...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-12-17
更新日期：2013-02-22 00:00:00
abstract：:Since falls are a major public health problem among older people, the number of systems aimed at detecting them has increased dramatically over recent years. This work presents an extensive literature review of fall detection systems, including comparisons among various kinds of studies. It aims to serve as a referenc...
journal_title：Biomedical engineering online
pub_type： 杂志文章,评审
doi：10.1186/1475-925X-12-66
更新日期：2013-07-06 00:00:00
abstract：BACKGROUND:Endovascular intervention using a stent is a mainstream treatment for cerebral aneurysms. To assess the effect of intervention strategies on aneurysm hemodynamics, we have developed a fast virtual stenting (FVS) technique to simulate stent deployment in patient-specific aneurysms. However, quantitative valid...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-016-0250-6
更新日期：2016-12-28 00:00:00
abstract：BACKGROUND:To determine how the mechanical property and micro structure affect tissue regeneration and angiogenesis, three types of scaffolds were studied. Acellular dermal matrices (ADM), produced from human skin by removing the epidermis and cells, has been widely used in wound healing because of its high mechanical ...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-12-125
更新日期：2013-12-04 00:00:00
abstract：BACKGROUND:This work deals with copyright protection of digital images, an issue that needs protection of intellectual property rights. It is an important issue with a large number of medical images interchanged on the Internet every day. So, it is a challenging task to ensure the integrity of received images as well a...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-015-0101-x
更新日期：2015-11-25 00:00:00
abstract：BACKGROUND:With the aging population and rising healthcare costs, wearable monitoring is gaining importance. The motion artifact affecting dry electrodes is one of the main challenges preventing the widespread use of wearable monitoring systems. In this paper we investigate the motion artifact and ways of making a text...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-12-26
更新日期：2013-04-08 00:00:00
abstract：BACKGROUND:Analysis of heart rate variation (HRV) has become a popular noninvasive tool for assessing the activities of the autonomic nervous system (ANS). HRV analysis is based on the concept that fast fluctuations may specifically reflect changes of sympathetic and vagal activity. It shows that the structure generati...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-3-24
更新日期：2004-07-20 00:00:00
abstract：BACKGROUND:The structure of the anatomical surfaces, e.g., CSF and gray and white matter, could severely influence the flow of volume currents in a head model. This, in turn, will also influence the scalp potentials and the inverse source localizations. This was examined in detail with four different human head models....
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-5-10
更新日期：2006-02-08 00:00:00
abstract：BACKGROUND:Effective fixation of fracture requires careful selection of a suitable implant to provide stability and durability. Implant with a feature of locking plate (LP) has been used widely for treating distal fractures in femur because of its favourable clinical outcome, but its potential in fixing proximal fractu...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-11-23
更新日期：2012-04-30 00:00:00
abstract：BACKGROUND:Irrigation is considered to be a critical part of root canal treatment. However, little is known about the effect of needle movement on the irrigation process. Therefore, this study aimed to investigate the influence of the syringe and needle movement on root canal irrigation using a three-dimensional comput...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-019-0679-5
更新日期：2019-05-06 00:00:00
abstract：BACKGROUND:Monitoring of vital parameters is an important topic in neonatal daily care. Progress in computational intelligence and medical sensors has facilitated the development of smart bedside monitors that can integrate multiple parameters into a single monitoring system. This paper describes non-contact monitoring...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-10-93
更新日期：2011-10-20 00:00:00
abstract：BACKGROUND:Xenogeneic bone has been widely used in a variety of clinical bone-related disease to promote bone healing and restore bone defects. However, the adverse effects of immune system limit its application in the clinic. The aim of this study was to evaluate xenogeneic bone safety of immunotoxicity and explore th...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-019-0729-z
更新日期：2019-11-14 00:00:00
abstract：BACKGROUND:The QRS complex is the most noticeable feature in the electrocardiogram (ECG) signal, therefore, its detection is critical for ECG signal analysis. The existing detection methods largely depend on hand-crafted manual features and parameters, which may introduce significant computational complexity, especiall...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-018-0441-4
更新日期：2018-01-29 00:00:00
abstract：BACKGROUND:In magnetic resonance electrical impedance tomography (MREIT), we reconstruct conductivity images using magnetic flux density data induced by externally injected currents. Since we extract magnetic flux density data from acquired MR phase images, the amount of measurement noise increases in regions of weak M...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-13-87
更新日期：2014-06-26 00:00:00
abstract：BACKGROUND:Newborn mammals suffering from moderate hypoxia during or after birth are able to compensate a transitory lack of oxygen by adapting their vital functions. Exposure to hypoxia leads to an increase in the sympathetic tone causing cardio-respiratory response, peripheral vasoconstriction and vasodilatation in p...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-10-88
更新日期：2011-10-03 00:00:00
abstract：BACKGROUND:Instrumented pendulum test is an objective and repeatable biomechanical method of assessment for spasticity. However, multitude of sensor technologies and plenty of suggested outcome measures, confuse those interested in implementing this method in practice. Lack of a standard agreement on the definition of ...
journal_title：Biomedical engineering online
pub_type： 杂志文章,评审
doi：10.1186/s12938-020-00826-8
更新日期：2020-11-09 00:00:00
abstract：BACKGROUND:It is a known fact that blood flow pattern and more specifically the pulsatile time variation of shear stress on the vascular wall play a key role in atherogenesis. The paper presents the conception, the building and the control of a new in vitro test bench that mimics the pulsatile flows behavior based on i...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-015-0065-x
更新日期：2015-08-07 00:00:00
abstract：BACKGROUND:The myocardium exhibits heterogeneous nature due to scarring after Myocardial Infarction (MI). In Cardiac Magnetic Resonance (CMR) imaging, Late Gadolinium (LG) contrast agent enhances the intensity of scarred area in the myocardium. METHODS:In this paper, we propose a probability mapping technique using Te...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-12-91
更新日期：2013-09-22 00:00:00
abstract：BACKGROUND:Robot-based joint-testing systems (RJTS) can be used to perform unconstrained laxity tests, measuring the stiffness of a degree of freedom (DOF) of the joint at a fixed flexion angle while allowing the other DOFs unconstrained movement. Previous studies using the force-position hybrid (FPH) control method pr...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-016-0195-9
更新日期：2016-06-07 00:00:00
abstract：BACKGROUND:Human embryonic stem cells (hESCs) are a potential source of cells for use in regenerative medicine. Automation of culturing, monitoring and analysis is crucial for fast and reliable optimization of hESC culturing methods. Continuous monitoring of living cell cultures can reveal more information and is faste...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-6-11
更新日期：2007-04-12 00:00:00
abstract：BACKGROUND:Biomedical named entity recognition (Bio-NER) is a fundamental task in handling biomedical text terms, such as RNA, protein, cell type, cell line, and DNA. Bio-NER is one of the most elementary and core tasks in biomedical knowledge discovery from texts. The system described here is developed by using the Bi...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-018-0573-6
更新日期：2018-11-06 00:00:00
abstract：BACKGROUND:STAR is a model-based, personalised, risk-based dosing approach for glycaemic control (GC) in critically ill patients. STAR provides safe, effective control to nearly all patients, using 1-3 hourly measurement and intervention intervals. However, the average 11-12 measurements per day required can be a clini...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-020-00771-6
更新日期：2020-04-29 00:00:00
abstract：BACKGROUND:Stroke is one of the major causes of morbidity and mortality. Its recovery and treatment depends on close clinical monitoring by a clinician especially during the first few hours after the onset of stroke. Patients who do not exhibit early motor recovery post thrombolysis may benefit from more aggressive tre...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-12-33
更新日期：2013-04-16 00:00:00
abstract：BACKGROUND:The main problem of tremor is the damage caused to the quality of the life of patients, especially those at more advanced ages. There is not a consensus yet about the origins of this disorder, but it can be examined in the correlations between the biological signs of aging and the tremor characteristics. ME...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-9-29
更新日期：2010-06-22 00:00:00
