Abstract：
BACKGROUND:Xenogeneic bone has been widely used in a variety of clinical bone-related disease to promote bone healing and restore bone defects. However, the adverse effects of immune system limit its application in the clinic. The aim of this study was to evaluate xenogeneic bone safety of immunotoxicity and explore the methods for immune risk supervision. RESULTS:Xenogeneic bone, which is freeze-dried bovine cancellous bone, was implanted into the muscle of mice. On day 7, 14 and 28, the effects of xenogeneic bone were examined on humoral immunity and cellular immunity, including the levels of IgG, IgM, C3, inflammatory factors (TNF-α, IL-6), alkaline phosphatase (ALP) and the lymphocyte phenotype. The data showed that xenogeneic bone implantation had no potential to induce immune responses not only in humoral immunity but also in cellular immunity. To reveal the risk of immunogenicity, the residual DNA and the clearance of α-gal epitope were analyzed in 2 different bones (bone 1 is deproteinized bone, bone 2 is acellular and defatted bone). It was suggested that DNA of xenogeneic bone can be limited to < 50 ng per mg dry weight for the repair or regeneration with the acceptable immune risk. And α-gal clearance of xenogeneic bone could be an effective risk factor for improving xenograft quality management. CONCLUSIONS:Through the detection of xenogeneic bone immunotoxicity, our findings indicated that the supervisions of risk factors could contribute to reduce the immune risk. And the risk factors under the acceptable limitation could decrease or replace animal experiment. However, it still needs to be studied on the limitation of α-gal epitope to predict rejection of xenogeneic bone more accurately.
journal_name
Biomed Eng Onlinejournal_title
Biomedical engineering onlineauthors
Sun X,Liu C,Shi Y,Li C,Sun L,Hou L,Wang Xdoi
10.1186/s12938-019-0729-zsubject
Has Abstractpub_date
2019-11-14 00:00:00pages
108issue
1issn
1475-925Xpii
10.1186/s12938-019-0729-zjournal_volume
18pub_type
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journal_title：Biomedical engineering online
pub_type： 杂志文章,评审
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journal_title：Biomedical engineering online
pub_type： 杂志文章
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journal_title：Biomedical engineering online
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journal_title：Biomedical engineering online
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journal_title：Biomedical engineering online
pub_type： 临床试验,杂志文章,多中心研究
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