Abstract：
BACKGROUND:Human embryonic stem cells (hESCs) are a potential source of cells for use in regenerative medicine. Automation of culturing, monitoring and analysis is crucial for fast and reliable optimization of hESC culturing methods. Continuous monitoring of living cell cultures can reveal more information and is faster than using laborious traditional methods such as microscopic evaluation, immunohistochemistry and flow cytometry. METHODS:We analyzed the growth dynamics of two hESC lines HS237 and HS293 in a conventional culture medium containing serum replacement and a xeno-free X-vivo 10 medium. We used a new automated culture platform utilizing machine vision technology, which enables automatic observation, recording and analysis of intact living cells. We validated the results using flow cytometry for cell counting and characterization. RESULTS:In our analyses, hESC colony growth could be continuously monitored and the proportion of undifferentiated cells automatically analyzed. No labeling was needed and we could, for the first time, perform detailed follow up of live, undisturbed cell colonies, and record all the events in the culture. The growth rate of the hESCs cultured in X-vivo 10 medium was significantly lower and a larger proportion of the cells were differentiated. CONCLUSION:The new automated system enables rapid and reliable analysis of undifferentiated growth dynamics of hESCs. We demonstrate the effectiveness of the system by comparing hESC growth in different culture conditions.
journal_name
Biomed Eng Onlinejournal_title
Biomedical engineering onlineauthors
Narkilahti S,Rajala K,Pihlajamäki H,Suuronen R,Hovatta O,Skottman Hdoi
10.1186/1475-925X-6-11subject
Has Abstractpub_date
2007-04-12 00:00:00pages
11issn
1475-925Xpii
1475-925X-6-11journal_volume
6pub_type
杂志文章abstract：BACKGROUND:Aging has been shown to slow reflexes and increase reaction time to varied stimuli. However, the effect of Type II diabetes on these same reaction times has not been reported. Diabetes affects peripheral nerves in the somatosensory and auditory system, slows psychomotor responses, and has cognitive effects o...
journal_title：Biomedical engineering online
pub_type： 临床试验,杂志文章
doi：10.1186/1475-925X-4-12
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journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-015-0116-3
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journal_title：Biomedical engineering online
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journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-11-81
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journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-11-80
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journal_title：Biomedical engineering online
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journal_title：Biomedical engineering online
pub_type： 杂志文章
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journal_title：Biomedical engineering online
pub_type： 杂志文章
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journal_title：Biomedical engineering online
pub_type： 杂志文章,撤回出版物
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journal_title：Biomedical engineering online
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journal_title：Biomedical engineering online
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journal_title：Biomedical engineering online
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journal_title：Biomedical engineering online
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journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-018-0603-4
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journal_title：Biomedical engineering online
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pub_type： 杂志文章
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journal_title：Biomedical engineering online
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journal_title：Biomedical engineering online
pub_type： 杂志文章,已发布勘误
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journal_title：Biomedical engineering online
pub_type： 杂志文章
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journal_title：Biomedical engineering online
pub_type： 杂志文章,评审
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更新日期：2013-03-09 00:00:00
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journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-6-32
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journal_title：Biomedical engineering online
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journal_title：Biomedical engineering online
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journal_title：Biomedical engineering online
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