Abstract:
BACKGROUND:Intracranial pressure changes during head impact cause brain injuries such as vasogenic edema and cerebral contusion. However, the influence of impulsive pressure on endothelial function has not yet been fully studied in vitro. In this study, we developed a pressure loading device that produced positive and negative pressures by modifying an in vitro fluid percussion model and examined the effects of the amplitude and duration of the pressures on endothelial permeability. METHODS:Human umbilical vein endothelial cells were subjected to three types of positive pressure (average amplitude/average duration of 352 kPa/23 ms, 73 kPa/27 ms, and 70 kPa/44 ms) and three types of negative pressure (-72 kPa/41 ms, -67 kPa/104 ms, and -91 kPa/108 ms), and the transendothelial electrical resistance (TEER) was measured between 15 min and 24 h after pressure loading for quantifying the formation of an integral monolayer of endothelial cells. After loading, vascular endothelial- (VE-) cadherin, an endothelium-specific cell-cell adhesion molecule involved in endothelial barrier function, was stained and observed using fluorescence microscopy. RESULTS:The pressure loading device could produce positive pressure pulses with amplitudes of 53-1348 kPa and durations of 9-29.1 ms and negative pressure pulses with amplitudes of -52 - -93 kPa and durations of 42.9-179.5 ms. The impulsive pressure reduced the TEER associated with the change in VE-cadherin localization. Additionally, TEER decreased considerably at 15 min and 6 h post-loading, with these changes being significant in positive pressure with larger amplitude and shorter duration and in all types of negative pressures compared to pre-loading. CONCLUSIONS:The changes in intracranial pressure during head impact impair endothelial barrier function by the disruption of the integrity of endothelial cell-cell junctions, and the degree of increase in endothelial permeability depends on the amplitude, duration, and direction (compressive and tensile) of the impulsive pressure.
journal_name
Biomed Eng Onlinejournal_title
Biomedical engineering onlineauthors
Nakadate H,Inuzuka K,Akanuma S,Kakuta A,Aomura Sdoi
10.1186/1475-925X-13-44subject
Has Abstractpub_date
2014-04-16 00:00:00pages
44issn
1475-925Xpii
1475-925X-13-44journal_volume
13pub_type
杂志文章abstract:BACKGROUND:The emergence and development of robot assistant interventional vascular surgery technologies have benefited many patients with cardiovascular or cerebrovascular diseases. Due to the absence of effective training measures, these new advanced technologies have not been fully utilized and only few experienced ...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/s12938-016-0148-3
更新日期:2016-04-06 00:00:00
abstract:BACKGROUND:A valveless pump generates a unidirectional net flow of fluid around a closed loop of soft viscoelastic tubing that is rhythmically compressed at one point. The tubing must have at least two sections with two different stiffnesses. When a short segment of the tube is squeezed asymmetrically at certain freque...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-9-42
更新日期:2010-08-31 00:00:00
abstract:BACKGROUND:Nonnegative matrix factorization (NMF) has been successfully used for electroencephalography (EEG) spectral analysis. Since NMF was proposed in the 1990s, many adaptive algorithms have been developed. However, the performance of their use in EEG data analysis has not been fully compared. Here, we provide a c...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/s12938-020-00796-x
更新日期:2020-07-31 00:00:00
abstract:BACKGROUND:The myocardium exhibits heterogeneous nature due to scarring after Myocardial Infarction (MI). In Cardiac Magnetic Resonance (CMR) imaging, Late Gadolinium (LG) contrast agent enhances the intensity of scarred area in the myocardium. METHODS:In this paper, we propose a probability mapping technique using Te...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-12-91
更新日期:2013-09-22 00:00:00
abstract:BACKGROUND:In a typical electrophysiological experiment, especially one that includes studying animal behavior, the data collected normally contain spikes, local field potentials, behavioral responses and other associated data. In order to obtain informative results, the data must be analyzed simultaneously with the ex...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/s12938-017-0419-7
更新日期:2017-11-13 00:00:00
abstract:BACKGROUND:Clinical diagnosis and therapy for the lumbar disc herniation requires accurate vertebra segmentation. The complex anatomical structure and the degenerative deformations of the vertebrae makes its segmentation challenging. METHODS:An improved level set method, namely edge- and region-based level set method ...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-12-48
更新日期:2013-05-28 00:00:00
abstract:BACKGROUND:Concerns about the cost-effectiveness of invasive hemodynamic monitoring in critically ill patients using pulmonary artery catheters motivate a renewed search for effective noninvasive methods to measure stroke volume. This paper explores a new approach based on noninvasively measured pulse wave velocity, pu...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-13-137
更新日期:2014-09-19 00:00:00
abstract:BACKGROUND:Optical imaging is one of the most common, low-cost imaging tools used for investigating the tumor biological behavior in vivo. This study explores the feasibility and sensitivity of a near infrared fluorescent protein mKate2 for a long-term non-invasive tumor imaging in BALB/c nude mice, by using a low-powe...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/s12938-018-0615-0
更新日期:2018-12-29 00:00:00
abstract:BACKGROUND:Classification of breast ultrasound (BUS) images is an important step in the computer-aided diagnosis (CAD) system for breast cancer. In this paper, a novel phase-based texture descriptor is proposed for efficient and robust classifiers to discriminate benign and malignant tumors in BUS images. METHOD:The p...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/s12938-015-0022-8
更新日期:2015-03-24 00:00:00
abstract:BACKGROUND:Red blood cells (RBCs) deform significantly and repeatedly when passing through narrow capillaries and delivering dioxygen throughout the body. Deformability of RBCs is a key characteristic, largely governed by the mechanical properties of the cell membrane. This study investigated RBC mechanical properties ...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/s12938-017-0429-5
更新日期:2017-12-19 00:00:00
abstract:BACKGROUND:Dendritic cells are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. The dendritic cell-based vaccination against cancer has been clinically achieved promising successes. But there are still many challenges ...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-13-2
更新日期:2014-01-10 00:00:00
abstract:BACKGROUND:One of the recommended treatments for disorders associated with the lower extremity venous insufficiency is the application of external mechanical compression. Compression stockings and elastic bandages are widely used for the purpose of compression therapy and are usually designed to exert a specified value...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/s12938-015-0088-3
更新日期:2015-11-05 00:00:00
abstract:BACKGROUND:Clinical studies suggest that local wall shear stress (WSS) patterns modulate the site and the progression of atherosclerotic lesions. Computational fluid dynamics (CFD) methods based on in-vivo three-dimensional vessel reconstructions have recently been shown to provide prognostically relevant WSS data. Thi...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-5-39
更新日期:2006-06-14 00:00:00
abstract:BACKGROUND:There is a high probability of accidental perforation of the vertebral pedicle wall in pedicle screw insertion surgery. A pedicle screw (PS) measurement device with an optical probe has been reported to send out a warning signal before the PS tip breaking the vertebral pedicle wall. METHODS:In this study, w...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-13-148
更新日期:2014-11-01 00:00:00
abstract:BACKGROUND:The usability of dexterous hand prostheses is still hampered by the lack of natural and effective control strategies. A decoding strategy based on the processing of descending efferent neural signals recorded using peripheral neural interfaces could be a solution to such limitation. Unfortunately, this choic...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/s12938-019-0659-9
更新日期:2019-04-08 00:00:00
abstract:BACKGROUND:The loss of the notochordal cells from the nucleus pulposus is associated with ageing and disc degeneration. However, understanding the mechanisms responsible for the loss of these cells has been hampered in part due to the difficulty of culturing and maintaining their phenotype. Furthermore, little is known...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-6-33
更新日期:2007-09-06 00:00:00
abstract:BACKGROUND:The replacement of hard tissues demands biocompatible and sometimes bioactive materials with properties similar to those of bone. Nano-composites made of biocompatible polymers and bioactive inorganic nano particles such as HDPE/HA have attracted attention as permanent bone substitutes due to their excellent...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-12-95
更新日期:2013-09-24 00:00:00
abstract:BACKGROUND:Non-contact tonometers based on the method using air puff and Scheimpflug's fast camera are one of the latest devices allowing the measurement of intraocular pressure and additional biomechanical parameters of the cornea. Biomechanical features significantly affect changes in intraocular pressure values, as ...
journal_title:Biomedical engineering online
pub_type: 杂志文章,评审
doi:10.1186/s12938-019-0636-3
更新日期:2019-02-13 00:00:00
abstract:BACKGROUND:Kinematics measures from inertial sensors have a value in the clinical assessment of pathological gait, to track quantitatively the outcome of interventions and rehabilitation programs. To become a standard tool for clinicians, it is necessary to evaluate their capability to provide reliable and comprehensib...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-13-146
更新日期:2014-10-21 00:00:00
abstract:BACKGROUND:Pre-operative imaging devices generate high-resolution images but intra-operative imaging devices generate low-resolution images. To use high-resolution pre-operative images during surgery, they must be deformed to reflect intra-operative geometry of brain. METHODS:We employ biomechanical models, guided by ...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-9-51
更新日期:2010-09-15 00:00:00
abstract:BACKGROUND:Computer-assisted arrhythmia recognition is critical for the management of cardiac disorders. Various techniques have been utilized to classify arrhythmias. Generally, these techniques classify two or three arrhythmias or have significantly large processing times. A simpler autoregressive modeling (AR) techn...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925x-1-5
更新日期:2002-11-13 00:00:00
abstract:BACKGROUND:Gait analysis and research have been developed to obtain characteristics of movement patterns of people while walking. However, traditional measuring systems present different drawbacks that reduce their use and application. Among those drawbacks one can find: high price, low sampling frequency and limiting ...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-13-34
更新日期:2014-03-31 00:00:00
abstract:INTRODUCTION:The method for measuring intraocular pressure using the Corvis tonometer provides a sequence of images of corneal deformation. Deformations of the cornea are recorded using the ultra-high-speed Scheimpflug camera. This paper presents a new and reproducible method of analysis of corneal deformation images t...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-13-150
更新日期:2014-11-19 00:00:00
abstract:BACKGROUND:The biomechanical simulation of the human respiratory system is expected to be a useful tool for the diagnosis and treatment of respiratory diseases. Because the deformation of the thorax significantly influences airflow in the lungs, we focused on simulating the thorax deformation by introducing contraction...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/s12938-016-0132-y
更新日期:2016-02-06 00:00:00
abstract:BACKGROUND:Endovascular intervention using a stent is a mainstream treatment for cerebral aneurysms. To assess the effect of intervention strategies on aneurysm hemodynamics, we have developed a fast virtual stenting (FVS) technique to simulate stent deployment in patient-specific aneurysms. However, quantitative valid...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/s12938-016-0250-6
更新日期:2016-12-28 00:00:00
abstract:BACKGROUND:Analysis of heart rate variation (HRV) has become a popular noninvasive tool for assessing the activities of the autonomic nervous system (ANS). HRV analysis is based on the concept that fast fluctuations may specifically reflect changes of sympathetic and vagal activity. It shows that the structure generati...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-3-24
更新日期:2004-07-20 00:00:00
abstract:BACKGROUND:Respiratory effort belt measurement is a widely used method to monitor respiration. Signal waveforms of respiratory volume and flow may indicate pathological signs of several diseases and, thus, it would be highly desirable to predict them accurately. Calibrated effort belts are sufficiently accurate for est...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-12-97
更新日期:2013-09-28 00:00:00
abstract:BACKGROUND:Stroke is one of the major causes of morbidity and mortality. Its recovery and treatment depends on close clinical monitoring by a clinician especially during the first few hours after the onset of stroke. Patients who do not exhibit early motor recovery post thrombolysis may benefit from more aggressive tre...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-12-33
更新日期:2013-04-16 00:00:00
abstract:BACKGROUND:In magnetic resonance electrical impedance tomography (MREIT), we reconstruct conductivity images using magnetic flux density data induced by externally injected currents. Since we extract magnetic flux density data from acquired MR phase images, the amount of measurement noise increases in regions of weak M...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-13-87
更新日期:2014-06-26 00:00:00
abstract:BACKGROUND:Human embryonic stem cell derived cardiomyocytes (hESC-CMs) hold high potential for basic and applied cardiovascular research. The development of a reliable simulation platform able to mimic the functional properties of hESC-CMs would be of considerable value to perform preliminary test complementing in vitr...
journal_title:Biomedical engineering online
pub_type: 杂志文章
doi:10.1186/1475-925X-11-61
更新日期:2012-08-28 00:00:00