Abstract：
BACKGROUND:Human embryonic stem cell derived cardiomyocytes (hESC-CMs) hold high potential for basic and applied cardiovascular research. The development of a reliable simulation platform able to mimic the functional properties of hESC-CMs would be of considerable value to perform preliminary test complementing in vitro experimentations. METHODS:We developed the first computational model of hESC-CM action potential by integrating our original electrophysiological recordings of transient-outward, funny, and sodium-calcium exchanger currents and data derived from literature on sodium, calcium and potassium currents in hESC-CMs. RESULTS:The model is able to reproduce basal electrophysiological properties of hESC-CMs at 15 40 days of differentiation (Early stage). Moreover, the model reproduces the modifications occurring through the transition from Early to Late developmental stage (50-110, days of differentiation). After simulated blockade of ionic channels and pumps of the sarcoplasmic reticulum, Ca2+ transient amplitude was decreased by 12% and 33% in Early and Late stage, respectively, suggesting a growing contribution of a functional reticulum during maturation. Finally, as a proof of concept, we tested the effects induced by prototypical channel blockers, namely E4031 and nickel, and their qualitative reproduction by the model. CONCLUSIONS:This study provides a novel modelling tool that may serve useful to investigate physiological properties of hESC-CMs.
journal_name
Biomed Eng Onlinejournal_title
Biomedical engineering onlineauthors
Paci M,Sartiani L,Del Lungo M,Jaconi M,Mugelli A,Cerbai E,Severi Sdoi
10.1186/1475-925X-11-61subject
Has Abstractpub_date
2012-08-28 00:00:00pages
61issn
1475-925Xpii
1475-925X-11-61journal_volume
11pub_type
杂志文章abstract：BACKGROUND:Knowing the orientation of the head is important in many fields, including medicine. Many methods and measuring systems exist, but usually they use different markers or sensors attached to the subject's head for head orientation determination. In certain applications these attachments may represent a burden ...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-12-96
更新日期：2013-09-26 00:00:00
abstract：BACKGROUND:Gene expression analyses demand appropriate reference genes (RGs) for normalization, in order to obtain reliable assessments. Ideally, RG expression levels should remain constant in all cells, tissues or experimental conditions under study. Housekeeping genes traditionally fulfilled this requirement, but the...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-017-0356-5
更新日期：2017-08-18 00:00:00
abstract：BACKGROUND:STAR is a model-based, personalised, risk-based dosing approach for glycaemic control (GC) in critically ill patients. STAR provides safe, effective control to nearly all patients, using 1-3 hourly measurement and intervention intervals. However, the average 11-12 measurements per day required can be a clini...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-020-00771-6
更新日期：2020-04-29 00:00:00
abstract：BACKGROUND:Fiber-based optical spectroscopy has been widely used for biomedical applications. However, the effect of probe-sample distance on the collection efficiency has not been well investigated. METHOD:In this paper, we presented a theoretical model to maximize the illumination and collection efficiency in design...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-10-95
更新日期：2011-11-02 00:00:00
abstract：BACKGROUND AND AIMS:People with internet addiction (IA) suffer from mental, physical, social, and occupational problems. IA includes psychological and physiological syndromes, and among the syndromes, emotion was suggested important mental and physiological expressions of IA. However, few physiologically emotional char...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-016-0201-2
更新日期：2016-07-04 00:00:00
abstract：BACKGROUND:In literature, the effect of the inflow boundary condition was investigated by examining the impact of the waveform and the shape of the spatial profile of the inlet velocity on the cardiac hemodynamics. However, not much work has been reported on comparing the effect of the different combinations of the inl...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-14-S1-S6
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND:In magnetic resonance electrical impedance tomography (MREIT), we reconstruct conductivity images using magnetic flux density data induced by externally injected currents. Since we extract magnetic flux density data from acquired MR phase images, the amount of measurement noise increases in regions of weak M...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-13-87
更新日期：2014-06-26 00:00:00
abstract：BACKGROUND:We sought to compare uterine cervical electrical impedance spectroscopy measurements employing two probes of different sizes, and to employ a finite element model to predict and compare the fraction of electrical current derived from subepithelial stromal tissue. METHODS:Cervical impedance was measured in 1...
journal_title：Biomedical engineering online
pub_type： 临床试验,杂志文章
doi：10.1186/1475-925X-5-62
更新日期：2006-11-24 00:00:00
abstract：BACKGROUND:The emergence and development of robot assistant interventional vascular surgery technologies have benefited many patients with cardiovascular or cerebrovascular diseases. Due to the absence of effective training measures, these new advanced technologies have not been fully utilized and only few experienced ...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-016-0148-3
更新日期：2016-04-06 00:00:00
abstract：BACKGROUND:Quantizing the Breast Imaging Reporting and Data System (BI-RADS) criteria into different categories with the single ultrasound modality has always been a challenge. To achieve this, we proposed a two-stage grading system to automatically evaluate breast tumors from ultrasound images into five categories bas...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-019-0626-5
更新日期：2019-01-24 00:00:00
abstract：BACKGROUND:In order to reduce the radiation dose of CT (computed tomography), compressed sensing theory has been a hot topic since it provides the possibility of a high quality recovery from the sparse sampling data. Recently, the algorithm based on DL (dictionary learning) was developed to deal with the sparse CT reco...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-016-0193-y
更新日期：2016-06-18 00:00:00
abstract：BACKGROUND:Aging has been shown to slow reflexes and increase reaction time to varied stimuli. However, the effect of Type II diabetes on these same reaction times has not been reported. Diabetes affects peripheral nerves in the somatosensory and auditory system, slows psychomotor responses, and has cognitive effects o...
journal_title：Biomedical engineering online
pub_type： 临床试验,杂志文章
doi：10.1186/1475-925X-4-12
更新日期：2005-02-21 00:00:00
abstract：BACKGROUND:Intracranial pressure changes during head impact cause brain injuries such as vasogenic edema and cerebral contusion. However, the influence of impulsive pressure on endothelial function has not yet been fully studied in vitro. In this study, we developed a pressure loading device that produced positive and ...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-13-44
更新日期：2014-04-16 00:00:00
abstract：:The Editors-in-Chief have retracted this article [1] because, after publication, concerns were raised regarding the study design and statistical analysis. Post-publication review has confirmed firstly that within-group changes were highlighted rather than between-group differences as appropriate for a randomized trial...
journal_title：Biomedical engineering online
pub_type： 杂志文章,撤回出版物
doi：10.1186/s12938-020-0749-8
更新日期：2020-01-24 00:00:00
abstract：BACKGROUND:One of the recommended treatments for disorders associated with the lower extremity venous insufficiency is the application of external mechanical compression. Compression stockings and elastic bandages are widely used for the purpose of compression therapy and are usually designed to exert a specified value...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-015-0088-3
更新日期：2015-11-05 00:00:00
abstract：BACKGROUND:Use of standardized and scientifically sound outcome measures is encouraged in clinical practice and research. With the development of newer rehabilitation therapies, we need technology-supported upper extremity outcome measures that are easily accessible, reliable and valid. 3-Dimensional printing (3D-print...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-020-00839-3
更新日期：2021-01-05 00:00:00
abstract：BACKGROUND:Studies found that treatment symptoms of concern to oncology/hematology patients were greatly under-identified in medical records. On average, 11.0 symptoms were reported of concern to patients compared to 1.5 symptoms identified in their medical records. A solution to this problem is use of an electronic sy...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-14-S2-S1
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND:Computer-assisted arrhythmia recognition is critical for the management of cardiac disorders. Various techniques have been utilized to classify arrhythmias. Generally, these techniques classify two or three arrhythmias or have significantly large processing times. A simpler autoregressive modeling (AR) techn...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925x-1-5
更新日期：2002-11-13 00:00:00
abstract：BACKGROUND:Speech production and speech phonetic features gradually improve in children by obtaining audio feedback after cochlear implantation or using hearing aids. The aim of this study was to develop and evaluate automated classification of voice disorder in children with cochlear implantation and hearing aids. ME...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-10-3
更新日期：2011-01-14 00:00:00
abstract：BACKGROUND:The lumbar range of motion has traditionally been used to assess disability in patients with low back disorders. Controversy exists about how movement ranges in static positions or in a single straight plane is related to the functional status of the patients. The trunk circumduction, as the result of neurom...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-12-4
更新日期：2013-01-11 00:00:00
abstract：BACKGROUND:We and others have previously demonstrated that adipose-derived stem cells (ASCs) transplantation improve cardiac dysfunction post-myocardium infarction (MI) under hemodynamic stress in rats. The beneficial effects appear to be associated with pleiotropic factors due to a complex interplay between the transp...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-13-54
更新日期：2014-05-01 00:00:00
abstract：BACKGROUND:In this study, we have examined local non-viral gene delivery, transfection, and therapeutic efficacy of endothelial nitric oxide synthase (eNOS) encoding plasmid DNA administered using coated stents in a rabbit iliac artery restenosis model. METHODS:Lipopolyplexes (LPPs) with eNOS expressing plasmid DNA we...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-9-56
更新日期：2010-09-27 00:00:00
abstract：BACKGROUND:A computational model of myocardial energy metabolism was used to assess the metabolic responses to normal and reduced myocardial blood flow. The goal was to examine to what extent glycolysis and lactate formation are controlled by the supply of glycolytic substrate and/or the cellular redox (NADH/NAD+) and ...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-3-15
更新日期：2004-06-02 00:00:00
abstract：BACKGROUND:To determine how the mechanical property and micro structure affect tissue regeneration and angiogenesis, three types of scaffolds were studied. Acellular dermal matrices (ADM), produced from human skin by removing the epidermis and cells, has been widely used in wound healing because of its high mechanical ...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-12-125
更新日期：2013-12-04 00:00:00
abstract：:Since falls are a major public health problem among older people, the number of systems aimed at detecting them has increased dramatically over recent years. This work presents an extensive literature review of fall detection systems, including comparisons among various kinds of studies. It aims to serve as a referenc...
journal_title：Biomedical engineering online
pub_type： 杂志文章,评审
doi：10.1186/1475-925X-12-66
更新日期：2013-07-06 00:00:00
abstract：BACKGROUND:Approximately one-third of the human lifespan is spent sleeping. To diagnose sleep problems, all-night polysomnographic (PSG) recordings including electroencephalograms (EEGs), electrooculograms (EOGs) and electromyograms (EMGs), are usually acquired from the patient and scored by a well-trained expert accor...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-11-52
更新日期：2012-08-21 00:00:00
abstract：BACKGROUND:Advances in transcranial electrical stimulation (tES) are hampered by the conventional rubber electrodes manually attached to the head with rubber bands. This procedure limits montages to a few electrodes, is error prone with respect to electrode configurations and is burdensome for participants and operator...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-020-00792-1
更新日期：2020-06-17 00:00:00
abstract：BACKGROUND:Phase contrast magnetic resonance imaging (PC-MRI) is used clinically for quantitative assessment of cardiovascular flow and function, as it is capable of providing directly-measured 3D velocity maps. Alternatively, vascular flow can be estimated from model-based computation fluid dynamics (CFD) calculations...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-015-0104-7
更新日期：2015-11-26 00:00:00
abstract：INTRODUCTION:The method for measuring intraocular pressure using the Corvis tonometer provides a sequence of images of corneal deformation. Deformations of the cornea are recorded using the ultra-high-speed Scheimpflug camera. This paper presents a new and reproducible method of analysis of corneal deformation images t...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-13-150
更新日期：2014-11-19 00:00:00
abstract：BACKGROUND:Clinical studies suggest that local wall shear stress (WSS) patterns modulate the site and the progression of atherosclerotic lesions. Computational fluid dynamics (CFD) methods based on in-vivo three-dimensional vessel reconstructions have recently been shown to provide prognostically relevant WSS data. Thi...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-5-39
更新日期：2006-06-14 00:00:00
