Abstract：
BACKGROUND:In this study, we have examined local non-viral gene delivery, transfection, and therapeutic efficacy of endothelial nitric oxide synthase (eNOS) encoding plasmid DNA administered using coated stents in a rabbit iliac artery restenosis model. METHODS:Lipopolyplexes (LPPs) with eNOS expressing plasmid DNA were immobilized on stainless steel stents using poly(D,L-lactide-co-glycolide) (PLGA) and type B gelatin coatings. The gene-eluting stents were implanted bilaterally in the denuded iliac arteries and eNOS transfection and therapeutic efficacy were examined 14 days after implantation. RESULTS:The results show that non-viral lipopolyplex-coated stents can efficiently tranfect eNOS locally in the arterial lumen assessed by PCR and ELISA. Human eNOS ELISA levels were significantly raised 24 hours after transfection compared to controls (125 pg eNOS compared to <50 pg for all controls including naked DNA). Local eNOS production suppressed smooth muscle cell proliferation and promoted re-endothelialization of the artery showing a significant reduction in restenosis of 1.75 neointima/media ratio for stents with lipoplexes encoding eNOS compared with 2.3 neointima/media ratio for stents with lipoplexes encosing an empty vector. CONCLUSIONS:These results support the hypothesis that a potent non-viral gene vector encoding for eNOS coated onto a stent can inhibit restenosis through inhibition of smooth muscle cell growth and promotion of a healthy endothelium.
journal_name
Biomed Eng Onlinejournal_title
Biomedical engineering onlineauthors
Brito LA,Chandrasekhar S,Little SR,Amiji MMdoi
10.1186/1475-925X-9-56subject
Has Abstractpub_date
2010-09-27 00:00:00pages
56issn
1475-925Xpii
1475-925X-9-56journal_volume
9pub_type
杂志文章abstract：OBJECTIVE:To evaluate the in vivo and in vitro performance of a China-made dialysis machine (SWS-4000). METHODS:This was a multi-center prospective controlled study consisting of both long-term in vitro evaluations and cross-over in vivo tests in 132 patients. The China-made SWS-4000 dialysis machine was compared with...
journal_title：Biomedical engineering online
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1186/s12938-017-0387-y
更新日期：2017-08-02 00:00:00
abstract：BACKGROUND:The emergence of skin substitutes provides a new approach for the treatment of wound repair and healing. The consistent and steady release of angiogenic factors is an important factor in the promotion of angiogenesis in skin substitutes, which usually lack, yet need, a vascular network. METHODS:In this stud...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-12-134
更新日期：2013-12-31 00:00:00
abstract：BACKGROUND:Retina degenerative disorders represent the primary cause of blindness in UK and in the developed world. In particular, Age Related Macular Degeneration (AMD) and Retina Pigmentosa (RP) diseases are of interest to this study. We have therefore created new image processing algorithms for enhancing the visual ...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-9-27
更新日期：2010-06-18 00:00:00
abstract：INTRODUCTION:This paper concerns the analysis of the features obtained from thyroid ultrasound images in left and right transverse and longitudinal sections. In the image analysis, the thyroid lobe is treated as a texture for healthy subjects and patients with Hashimoto's disease. The applied methods of analysis and im...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-11-48
更新日期：2012-08-16 00:00:00
abstract：:A large number of incommensurable metrics are currently used to report the performance of brain-computer interfaces (BCI) used for augmentative and alterative communication (AAC). The lack of standard metrics precludes the comparison of different BCI-based AAC systems, hindering rapid growth and development of this te...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-12-43
更新日期：2013-05-16 00:00:00
abstract：:Advances in biomaterials and the need for patient-specific bone scaffolds require modern manufacturing approaches in addition to a design strategy. Hybrid materials such as those with functionally graded properties are highly needed in tissue replacement and repair. However, their constituents, proportions, sizes, con...
journal_title：Biomedical engineering online
pub_type： 杂志文章,评审
doi：10.1186/s12938-020-00810-2
更新日期：2020-09-03 00:00:00
abstract：BACKGROUND:The bioluminescent enzyme firefly luciferase (Luc) or variants of green fluorescent protein (GFP) in transformed cells can be effectively used to reveal molecular and cellular features of neoplasia in vivo. Tumor cell growth and regression in response to various therapies can be evaluated by using biolumines...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-3-12
更新日期：2004-05-04 00:00:00
abstract：BACKGROUND:The gait movement is an essential process of the human activity and the result of collaborative interactions between the neurological, articular and musculoskeletal systems, working efficiently together. This explains why gait analysis is important and increasingly used nowadays for the diagnosis of many dif...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-015-0097-2
更新日期：2015-10-29 00:00:00
abstract：BACKGROUND:Regional differences in action potential duration (APD) restitution in the heart favour arrhythmias, but the mechanism is not well understood. METHODS:We simulated a 150 x 150 mm 2D sheet of cardiac ventricular tissue using a simplified computational model. We investigated wavebreak and re-entry initiated b...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-4-54
更新日期：2005-09-20 00:00:00
abstract：BACKGROUND:The paper presents an approach and computer-aided method of numerical evaluation of the quality of porous membranes used as scaffolds for cultivation of chondrocytes in regeneration of biological tissues. MATERIALS:The scanning electron microscope (SEM) images of 300× and 1000× magnification presenting the ...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-018-0481-9
更新日期：2018-05-30 00:00:00
abstract：BACKGROUND:The main problem of tremor is the damage caused to the quality of the life of patients, especially those at more advanced ages. There is not a consensus yet about the origins of this disorder, but it can be examined in the correlations between the biological signs of aging and the tremor characteristics. ME...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-9-29
更新日期：2010-06-22 00:00:00
abstract：BACKGROUND:Nonnegative matrix factorization (NMF) has been successfully used for electroencephalography (EEG) spectral analysis. Since NMF was proposed in the 1990s, many adaptive algorithms have been developed. However, the performance of their use in EEG data analysis has not been fully compared. Here, we provide a c...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-020-00796-x
更新日期：2020-07-31 00:00:00
abstract：BACKGROUND:Advances in transcranial electrical stimulation (tES) are hampered by the conventional rubber electrodes manually attached to the head with rubber bands. This procedure limits montages to a few electrodes, is error prone with respect to electrode configurations and is burdensome for participants and operator...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-020-00792-1
更新日期：2020-06-17 00:00:00
abstract：BACKGROUND:One of the recommended treatments for disorders associated with the lower extremity venous insufficiency is the application of external mechanical compression. Compression stockings and elastic bandages are widely used for the purpose of compression therapy and are usually designed to exert a specified value...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-015-0088-3
更新日期：2015-11-05 00:00:00
abstract：BACKGROUND:Maximum Intensity Projections (MIP) of neuronal dendritic trees obtained from confocal microscopy are frequently used to study the relationship between tree morphology and mechanosensory function in the model organism C. elegans. Extracting dendritic trees from noisy images remains however a strenuous proces...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-13-74
更新日期：2014-06-12 00:00:00
abstract：BACKGROUND:Assessing hand injury is of great interest given the level of involvement of the hand with the environment. Knowing different assessment systems and their limitations generates new perspectives. The integration of digital systems (accelerometry and electromyography) as a tool to supplement functional assessm...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-13-161
更新日期：2014-12-11 00:00:00
abstract：BACKGROUND:Rotational osteotomy is frequently indicated to correct excessive femoral anteversion in cerebral palsy patients. Angled blade plate is the standard fixation device used when performed in the proximal femur, but extensile exposure is required for plate accommodation. The authors developed a short locked intr...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-10-57
更新日期：2011-06-28 00:00:00
abstract：PURPOSE:We propose a collaborative and secure transmission scheme in order to safely and efficiently transmit medical data and provide telemedicine services, lighten the load on wireless access networks, and improve the quality of medical treatment such as surgery. METHODS:First, the transmission technology based on o...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-019-0674-x
更新日期：2019-05-20 00:00:00
abstract：BACKGROUND:Xenogeneic bone has been widely used in a variety of clinical bone-related disease to promote bone healing and restore bone defects. However, the adverse effects of immune system limit its application in the clinic. The aim of this study was to evaluate xenogeneic bone safety of immunotoxicity and explore th...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-019-0729-z
更新日期：2019-11-14 00:00:00
abstract：BACKGROUND:Good anatomical compatibility is an important aspect in the development of cardiovascular implants. This work analyzes the interaction of the pump unit of an electrically driven pulsatile Total Artificial Heart (TAH) and the mediastinum. For an adequate compliance, both overall dimensions and alignment of in...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-12-81
更新日期：2013-08-14 00:00:00
abstract：BACKGROUND:This study investigates CAD/CAM ceramic cusp-replacing restoration resistance with and without buccal cusp replacement under static and dynamic cyclic loads, monitored using the acoustic emission (AE) technique. METHOD:The cavity was designed in a typical MODP (mesial-occlusal-distal-palatal) restoration fa...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-13-165
更新日期：2014-12-13 00:00:00
abstract：BACKGROUND:The subjects in EEG-Brain computer interface (BCI) system experience difficulties when attempting to obtain the consistent performance of the actual movement by motor imagery alone. It is necessary to find the optimal conditions and stimuli combinations that affect the performance factors of the EEG-BCI syst...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-10-91
更新日期：2011-10-12 00:00:00
abstract：BACKGROUND:Current devices for measuring muscle contraction in vivo have limited accuracy in establishing and re-establishing the optimum muscle length. They are variable in the reproducibility to determine the muscle contraction at this length, and often do not maintain precise conditions during the examination. Conse...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-6-1
更新日期：2007-01-02 00:00:00
abstract：BACKGROUND:Muscle fiber orientation (MFO) is an important parameter related to musculoskeletal functions. The traditional manual method for MFO estimation in sonograms was labor-intensive. The automatic methods proposed in recent years also involved voting procedures which were computationally expensive. METHODS:In th...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-12-98
更新日期：2013-09-30 00:00:00
abstract：BACKGROUND:Respiratory effort belt measurement is a widely used method to monitor respiration. Signal waveforms of respiratory volume and flow may indicate pathological signs of several diseases and, thus, it would be highly desirable to predict them accurately. Calibrated effort belts are sufficiently accurate for est...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-12-97
更新日期：2013-09-28 00:00:00
abstract：BACKGROUND:In stent design optimization, the functional relationship between design parameters and design goals is nonlinear, complex, and implicit and the multi-objective design of stents involves a number of potentially conflicting performance criteria. Therefore it is hard and time-consuming to find the optimal desi...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-016-0268-9
更新日期：2016-12-28 00:00:00
abstract：BACKGROUND:It is generally accepted that cartilage adaptation and degeneration are mechanically mediated. Investigating the swelling behaviour of cartilage is important because the stress and strain state of cartilage is associated with the swelling and deformation behaviour. It is well accepted that the swelling of so...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925x-1-8
更新日期：2002-12-19 00:00:00
abstract：BACKGROUND:The anterior cruciate ligament (ACL) plays an important role in stabilizing translation and rotation of the tibia relative to the femur. ACL injury alters knee kinematics and usually links to the alternation of gait patterns. The aim of this study is to develop a new method to distinguish between gait patter...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-018-0594-1
更新日期：2018-11-01 00:00:00
abstract：BACKGROUND:Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal tumors and usually presented with locally advanced and distant metastasis disease, which prevent curative resection or treatments. In this regard, we considered identifying molecular subtypes associated with clinicopathological factor as progn...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/1475-925X-13-S2-S5
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Inhaled nitric oxide (NO) is a selective pulmonary vasodilator used primarily in the critical care setting for patients concurrently supported by invasive or noninvasive positive pressure ventilation. NO delivery devices interface with ventilator breathing circuits to inject NO in proportion with the flow of...
journal_title：Biomedical engineering online
pub_type： 杂志文章
doi：10.1186/s12938-016-0227-5
更新日期：2016-08-30 00:00:00
