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Short-term mechanical stretch fails to differentiate human adipose-derived stem cells into cardiovascular cell phenotypes.

Abstract：

BACKGROUND:We and others have previously demonstrated that adipose-derived stem cells (ASCs) transplantation improve cardiac dysfunction post-myocardium infarction (MI) under hemodynamic stress in rats. The beneficial effects appear to be associated with pleiotropic factors due to a complex interplay between the transplanted ASCs and the microenvironment in the absence of cell transdifferentiation. In the present work, we tested the hypothesis that mechanical stretch per se could change human ASCs (hASCs) into cardiovascular cell phenotypes that might influence post-MI outcomes. METHODS:Human ASCs were obtained from patients undergoing liposuction procedures. These cells were stretched 12%, 1Hz up to 96 hours by using Flexercell 4000 system. Protein and gene expression were evaluated to identify cardiovascular cell markers. Culture medium was analyzed to determine cell releasing factors, and contraction potential was also evaluated. RESULTS:Mechanical stretch, which is associated with extracellular signal-regulated kinase (ERK) phosphorylation, failed to induce the expression of cardiovascular cell markers in human ASCs, and mesenchymal cell surface markers (CD29; CD90) remained unchanged. hASCs and smooth muscle cells (SMCs) displayed comparable contraction ability. In addition, these cells demonstrated a profound ability to secrete an array of cytokines. These two properties of human ASCs were not influenced by mechanical stretch. CONCLUSIONS:Altogether, our findings demonstrate that hASCs secrete an array of cytokines and display contraction ability even in the absence of induction of cardiovascular cell markers or the loss of mesenchymal surface markers when exposed to mechanical stretch. These properties may contribute to beneficial post-MI cardiovascular outcomes and deserve to be further explored under the controlled influence of other microenvironment components associated with myocardial infarction, such as tissue hypoxia.

journal_name

Biomed Eng Online

journal_title

Biomedical engineering online

authors

Girão-Silva T,Bassaneze V,Campos LC,Barauna VG,Dallan LA,Krieger JE,Miyakawa AA

doi

10.1186/1475-925X-13-54

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

issn

1475-925X

pii

1475-925X-13-54

journal_volume

pub_type

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Short-term mechanical stretch fails to differentiate human adipose-derived stem cells into cardiovascular cell phenotypes.