Abstract:
:Tranilast is used clinically as a drug for hypertrophic scars or keloids. Recently, the roles of keratinocytes in the pathogenesis of those conditions have been noted. Therefore, we first examined the effect of tranilast on the cell growth of normal human keratinocytes. A cell growth assay demonstrated that the cell number significantly decreased during 48 h cultures with the addition of tranilast (5-400 μM) compared with a control (tranilast 0) in a dose-dependent manner. Morphologically, cell spreading was decreased and the cell body was elongated with higher concentrations (200-400 μM) of tranilast, and the cell area decreased significantly. The effect was not due to cytotoxicity. The inhibition of cell growth and the changes in cell morphology by the treatment of 100 μM tranilast reversed after the removal of the tranilast. Immunohistochemical staining revealed that F-actin and vinculin expression with tranilast-treated keratinocytes decreased significantly in a dose-dependent manner (100-400 μM). In addition, cell cycle examination showed that 400 μM of tranilast caused G0/G1 arrest with the keratinocytes. From these data we concluded that tranilast inhibited the growth of normal human keratinocytes, and one of its mechanisms may involve decreasing cell spreading by inhibition of F-actin fiber and focal contact formation with the cells.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Kubo M,Zhao Y,Moriguchi Tdoi
10.1007/s00403-012-1291-8subject
Has Abstractpub_date
2012-11-01 00:00:00pages
745-53issue
9eissn
0340-3696issn
1432-069Xjournal_volume
304pub_type
杂志文章abstract::To examine the induction and repair of UV-induced DNA damage, indirect immunofluorescence was performed on UVB-irradiated organ-cultured normal human skin using monoclonal antibodies specific for either cyclobutane pyrimidine dimers or (6-4) photoproducts. Nuclear immunofluorescence of cyclobutane pyrimidine dimers an...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00375800
更新日期:1992-01-01 00:00:00
abstract::Bone morphogenetic proteins (BMPs) and activins are phylogenetically conserved proteins, belonging to the transforming growth factor-β superfamily, that signal through the phosphorylation of receptor-regulated Smad proteins, activating different cell responses. They are involved in various steps of skin morphogenesis ...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/s00403-013-1381-2
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abstract::The calcipotriol/betamethasone dipropionate fixed-combination gel is widely used for topical treatment of psoriasis vulgaris. It has been hypothesized that calcipotriol counteracts glucocorticoid-induced skin atrophy which is associated with changes in the extracellular matrix (ECM). To elucidate the combined effects ...
journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417712
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abstract::Patients with atopic dermatitis (AD) often present with dry skin, and the reduced secretion of sebum may be responsible for the impaired skin barrier function. A sebum check film enables the patient to self-evaluate the skin sebum content. This study compared the sebum check film with a sebumeter. The skin sebum conte...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-010-1076-x
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abstract::The dependence of epithelial spread on human serum in culture has been studied. Using measured pieces of mouse ear skin epithelial outgrowth about floating explants (epiboly) and from adherent explants was studied. Where compared directly the two systems show similar results. Because of its ease of quantitation, the a...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417274
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abstract::When determined in vitro, the SPF of certain commercial sunscreen products can be lower than the SPF indicated on the label. The study of the composition of this type of product enabled us to note that the majority contained substances reputed to have anti-inflammatory properties. This effect is shown by inhibiting th...
journal_title:Archives of dermatological research
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doi:10.1007/s00403-012-1257-x
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-015-1581-z
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
pub_type: 杂志文章
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-015-1582-y
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00412706
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abstract::A 50-year-old male patient had three episodes of acrally located purpura within 12 years without any other symptoms. The first two episodes which lasted several months coincided with the intake of beta-blockers; the third episode developed after a respiratory infection. A cryofibrinogenemia was found without any evide...
journal_title:Archives of dermatological research
pub_type: 杂志文章
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00509062
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:
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journal_title:Archives of dermatological research
pub_type: 杂志文章
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00493461
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journal_title:Archives of dermatological research
pub_type: 杂志文章
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journal_title:Archives of dermatological research
pub_type: 杂志文章
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journal_title:Archives of dermatological research
pub_type: 杂志文章
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journal_title:Archives of dermatological research
pub_type: 杂志文章
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00404292
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF02505240
更新日期:1996-07-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
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