Abstract：
:The dependence of epithelial spread on human serum in culture has been studied. Using measured pieces of mouse ear skin epithelial outgrowth about floating explants (epiboly) and from adherent explants was studied. Where compared directly the two systems show similar results. Because of its ease of quantitation, the adherent explant culture was studied in greater detail. In this system in the presence of serum appreciable spread was found only after 48 h but spread continued for at least the next 4 days. In the absence of serum only minimal epithelial spread occurred. Adding serum to deficient media enhanced spreading and removing serum from media depressed spreading. The extent of spread appeared independent of mouse age for the first 4-10 weeks though 2-week-old mouse skin showed quantitatively greater spreading. The activity in human serum responsible for epithelial spread acts under conditions of minimal DNA synthesis and is not reproduced by bovine serum albumin, fetuin, or serum that had been exposed to 100 degrees C for 5 min. The activity is not dialyzable and it is resistant to the protease inhibitors DFP and PMSF. These studies suggest that a specific serum component(s) serves to support epithelial spread in vitro.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Stenn KS,Dvoretzky Idoi
10.1007/BF00417274subject
Has Abstractpub_date
1979-02-23 00:00:00pages
3-15issue
1eissn
0340-3696issn
1432-069Xjournal_volume
264pub_type
杂志文章abstract：:The exposure of naked unprotected skin to solar radiation may result in numerous acute and chronic undesirable effects. Evidence suggests that silymarin, a standardized extract from Silybum marianum (L.) Gaertn. seeds, and its major component silybin suppress UVB-induced skin damage. Here, we aimed to investigate the ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-019-01928-7
更新日期：2019-08-01 00:00:00
abstract：:Paraneoplastic pemphigus patients (PNP) develop a group of autoantibodies, among which those against envoplakin and periplakin are almost always found. Epitope mapping has indicated that the linker subdomains of the proteins harbor the major antigenic sites recognized by PNP sera. In order to detect specific autoantib...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-008-0901-y
更新日期：2009-10-01 00:00:00
abstract：:The internal stratum corneum lipid composition was investigated in relation to depth in vivo in healthy human volunteers by extraction following one, three or five strippings. Automated multiple development high-performance thin-layer chromatography (AMD-HPTLC) and gas chromatography (GC) followed by normalized princi...
journal_title：Archives of dermatological research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/s004030050158
更新日期：1997-01-01 00:00:00
abstract：:For the better part of the past century, dermatologists have regarded the skin primarily as a large protective coat. Epidermal keratinocytes were highlighted for their production of keratins and lipids, which contributed to the structural integrity and barrier formation of skin. This "saran-wrap" perspective of skin m...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/BF00638233
更新日期：1992-01-01 00:00:00
abstract：:Mice homozygous for three different recessive mutations known to cause pleiotropic defects in the immune system and in the skin were used to evaluate the relationship between the classical immune system and dendritic epidermal cell populations. Numbers of Langerhans cells (LCs) and Thy-1+ dendritic cells (Thy-1+DEC) w...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00372621
更新日期：1990-01-01 00:00:00
abstract：:Interleukin-17 (IL-17) has been implicated in the pathogenesis of a large number of inflammatory and autoimmune conditions, including skin disorders such as psoriasis. Recently, much data have accumulated on the possible role of IL-17 in the pathogenesis of alopecia areata (AA). In this review, the available informati...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/s00403-018-1823-y
更新日期：2018-07-01 00:00:00
abstract：BACKGROUND:Anogenital warts are a common human papillomavirus infection. They cause emotional distress, especially when they are in the anogenital region. Cryotherapy is a first-line treatment. Previous clinical trials and case series have reported variable results with retinoids (isotretinoin) as adjuvant therapy. OB...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-020-02182-y
更新日期：2021-01-12 00:00:00
abstract：:The C1-inactivator plays an important role not only in the initial phases of the complement system, but also in those of the coagulation, fibrinolysis and kinin systems. The present study was concerned with the reciprocal influence of decreased C1-inactivator levels in patients with hereditary angioneurotic edema (HAN...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00413358
更新日期：1984-01-01 00:00:00
abstract：:A low-molecular-weight proteinase inhibitor was isolated from human epidermal extracts by means of ultrafiltration, gel filtration and ion-exchange chromatography. On polyacrylamide gel electrophoresis (PAGE), the isolated fraction exhibited a single band of activity at pH 9.4, and inhibitory activity against papain a...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00412923
更新日期：1986-01-01 00:00:00
abstract：:Topical vitamin D3 has relatively recently been introduced for the treatment of psoriasis. Synthetic vitamin D3 analogues with a high potential for inducing differentiation of cells, but with a low hypercalcemic effect have recently been developed. One such synthetic analogue of 1,25-dihydroxyvitamin D3 (calcitriol), ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050444
更新日期：1999-09-01 00:00:00
abstract：:The demonstration of cytokine mRNA expression in epidermal cells by the polymerase chain reaction technique preceded by reverse transcription (RT-PCR) requires linear test conditions (i.e. that the product obtained after amplification reflects the relative amounts of starting material) and high reproducibility, specif...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00370722
更新日期：1994-01-01 00:00:00
abstract：:An increase in phosphodiesterase 4 (PDE4) in blood mononuclear white cells of patients with atopic dermatitis (AD) has been described. This and other skin disorders worsen during stress, during which there are increased circulating levels of adrenaline and glucocorticoids. The aim of this study was to analyse the effe...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-002-0313-3
更新日期：2002-07-01 00:00:00
abstract：:Organ size regulation is a highly coordinated process involving complex mechanisms. Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif, also known as WWTR1 (TAZ) have recently been linked to organ size determination and cell proliferation. Pilomatrixoma (PM) is a benign tumor of the a...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-014-1530-2
更新日期：2015-05-01 00:00:00
abstract：:Bromodeoxyuridine (BrdU) has commonly been used for detecting of label-retaining cells (LRCs). To determine if there are LRCs and the distributions of LRCs in eccrine sweat glands, 20 newborn SD rats within 24 h after birth were injected intraperitoneally with 50 mg/kg/time BrdU four consecutive times at 2-h intervals...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-013-1397-7
更新日期：2014-03-01 00:00:00
abstract：:Epidermolytic hyperkeratosis (EH) is a genetic disorder of keratins associated with epidermal differentiation. Affected individuals carry gene mutations for conserved sequences of keratins K1 or K10. The structural alterations of tonofilaments in EH seem to be a direct consequence of the keratin gene mutations. EH epi...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF01105793
更新日期：1995-01-01 00:00:00
abstract：:Comparative measurements of collagen synthesis as a portion of total protein synthesis were performed with fragments of peripheral neurofibromas, of skin adjacent to the tumors, and of unaffected skin from patients with neurofibromatosis (NF). No significant difference was found among these various samples. Collagen s...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00412930
更新日期：1986-01-01 00:00:00
abstract：:The aim of this investigation was to determine whether volumetric changes occurred in differentiating layers of stratified squamous epithelium. Samples of cheek pouch mucosa from 5 hamsters were obtained, prepared for electron microscopy using carefully controlled methods, and electron micrographs obtained from define...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/BF00409260
更新日期：1982-01-01 00:00:00
abstract：:The aim of this study was to screen the antioxidant, anti-hyaluronidase, and anti-elastase activity of the lyophilized juice of Cucumis sativus fruit (CSLJ). The CSLJ was subjected to DPPH and superoxide radical scavenging assay in reference to butylated hydroxytoluene. The hyaluronidase and elastase inhibitory assay ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-010-1103-y
更新日期：2011-05-01 00:00:00
abstract：:The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) and epidermal growth factor (EGF) on type-IV-procollagen (basement-membrane collagen) and laminin synthesis, turnover, and secretion was studied in human A431 squamous epidermoid carcinoma cells. Type-IV procollagen and laminin were biochemically and immunologic...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00509237
更新日期：1985-01-01 00:00:00
abstract：:Skin topography and microvasculature undergo characteristic changes with age. Although several non-invasive bioengineering methods are currently available to measure them quantitatively, few publications have referred to their relationship with age in different anatomical sites. This study was carried out to observe t...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-005-0628-y
更新日期：2006-03-01 00:00:00
abstract：:This study was designed to investigate the effects of griseofulvin, 5-fluorocytosine (5-FC), and amphotericin B on pancreatic and gastric secretion after the administration of a single large dose, or repeated small doses for 3 weeks, in male albino rats. The exocrine functions of the main digestive glands were only sl...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00694617
更新日期：1979-10-01 00:00:00
abstract：:The prolongation of the S-phase, characteristic of psoriatic keratinocytes, can be observed in cells of the eccrine sweat glands as well as in those of the infundibular part of hair follicles. In healthy individuals the average duration of DNA-synthesis time (ts) in the cells of the hair follicle is 6.8 h, in unaffect...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00404286
更新日期：1980-01-01 00:00:00
abstract：:Mast cell chymase is a chymotrypsin-like serine proteinase primarily stored in secretory mast cell granules. Mast cell chymase has various effects on angiotensin, metalloproteases, lipoproteins, procollagen, neuropeptides and cytokines. Recent studies have demonstrated that chymase inhibitors inhibit skin inflammation...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-005-0542-3
更新日期：2005-04-01 00:00:00
abstract：:The effects of acidic and basic fibroblast growth factors (aFGF and bFGF, respectively) on proliferation and differentiation of cultured hair apparatus cells were examined using bovine pituitary extract (BPE)-free media. The importance of FGFs as growth factors in BPE was also examined. Both FGFs and BPE stimulated ce...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00371577
更新日期：1994-01-01 00:00:00
abstract：:We investigated the effect of interferon-gamma (IFN-gamma) on skin equivalents. Keratinocytes from involved and uninvolved skin from psoriatic subjects and from healthy subjects were grown on preproduced dermal equivalents (DE) containing fibroblasts from healthy skin or psoriatic lesions. Healthy keratinocytes were a...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050146
更新日期：1996-12-01 00:00:00
abstract：:Autoimmune diseases can be preceded by a symptom-free phase which is defined by the presence of autoantibodies, and may last for many years. These autoantibodies may have a high positive predictive value for disease onset, severity and organ-specific complications, especially in genetically prone individuals. Characte...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/s00403-008-0889-3
更新日期：2009-01-01 00:00:00
abstract：:Survivin, a member of the inhibitors of apoptosis protein family, regulates both cellular proliferation and apoptotic cell death. While the human survivin gene is highly expressed in the developing fetus, in adults its expression is restricted to highly proliferating normal tissues and neoplastic tumors tissues. In th...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-005-0572-x
更新日期：2005-07-01 00:00:00
abstract：:Bullous pemphigoid (BP) is a chronic debilitating autoimmune blistering disease that frequently occurs in the elderly population. Previous studies have suggested a high morbidity and mortality associated with BP. However, relatively few studies have investigated prognostic factors of BP mortality, and they showed cons...
journal_title：Archives of dermatological research
pub_type： 杂志文章,meta分析
doi：10.1007/s00403-017-1736-1
更新日期：2017-07-01 00:00:00
abstract：:It is known that eosinophils are actively involved in allergy and inflammation. The granular components of eosinophils, eosinophil cationic protein (ECP) and eosinophil-derived neurotoxin/eosinophil protein X (EDN/EPX), play an important role in such allergic and inflammatory processes. Prurigo nodularis is a chronic ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030000142
更新日期：2000-08-01 00:00:00
abstract：:Skin aging is a natural process of the human body that may be accelerated due to extrinsic causes. Libidibia ferrea, popularly known as jucá, is a small tree, which possesses an abundant phenolic composition with potential antioxidant and enzymatic inhibition activities. Thus, this work aimed to investigate the anti-w...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-016-1685-0
更新日期：2016-11-01 00:00:00
