Abstract：
:The aim of this investigation was to determine whether volumetric changes occurred in differentiating layers of stratified squamous epithelium. Samples of cheek pouch mucosa from 5 hamsters were obtained, prepared for electron microscopy using carefully controlled methods, and electron micrographs obtained from defined basal, spinous and granular layers of the epithelium. Stereological point counting procedures were used to determine the ratio of nucleus to cytoplasm for each of the defined cell layers. From direct measurement of nuclear profiles, major and minor axes were transformed to diameters of circles of equivalent area and it was thus possible to obtain an estimate of nuclear volume. Using the previously determined nuclear-cytoplasmic ratio, the volume of cytoplasm and hence cell volume, could be estimated for the cell layers. Between basal and granular layers, nuclear-cytoplasmic ratios decreased from 0.42 to 0.08, whereas cytoplasmic and cellular volumes increased progressively from 248 to 1052 microns 3 and from 352 to 1144 microns 3, respectively. Nuclear axial ratios were highest in the granular layers. These methods can be used in a variety of comparative ultrastructural studies of epithelia, and will also prove valuable in generating additional biological information from more conventionally presented stereological data.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
White FH,Gohari Kdoi
10.1007/BF00409260subject
Has Abstractpub_date
1982-01-01 00:00:00pages
307-18issue
3-4eissn
0340-3696issn
1432-069Xjournal_volume
273pub_type
杂志文章,评审abstract：:Psoriasis was initially considered to represent a disease of abnormal epidermal keratinocyte proliferation. Proliferation of keratinocytes is restricted by apoptotic cell death to maintain a constant thickness of epidermis. Nucleosomes are mainly released by apoptotic cells. Tumor necrosis factor-α (TNF-α) is an impor...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-013-1347-4
更新日期：2013-08-01 00:00:00
abstract：:Genetic differences are involved in the development of lupus erythematosus (LE). Skin lesions are influenced by environmental triggers such as ultraviolet light, temperature, and chemical stresses, and the patterns of skin lesion are variable in cutaneous LE such as systemic LE (SLE), chronic discoid LE (CDLE), subacu...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/s00403-008-0897-3
更新日期：2009-01-01 00:00:00
abstract：:Fast antigen is a cell surface protein that mediates apoptosis. Using immunohistological, flow cytometry and electron microscopic analyses, we investigated the expression of Fas antigen on various skin tissues, and on cultured SV40-transformed human epidermal keratinocyte cell line KJD and human skin squamous cell car...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00371800
更新日期：1994-01-01 00:00:00
abstract：BACKGROUND:Data on how patients with a history of skin cancer apply sunscreen are lacking. OBJECTIVE:To characterize (1) gender differences in sunscreen application technique (quantity used, anatomic site coverage, and time allocated) and (2) differences in sunscreen application to unaffected skin versus previous skin...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-020-02131-9
更新日期：2020-12-01 00:00:00
abstract：:Vitiliginous patients undergoing photochemotherapy with TMP were less susceptible to a sun burn. To investigate this point, the MED for UV-B was measured before, 2 h, and 24 h after oral intake of TMP in 10 healthy volunteers. There was an increase of MED when UV exposure had taken place 2 h after TMP intake (P < 0.01...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00403890
更新日期：1980-01-01 00:00:00
abstract：:Linoleic acid is required for the formation and maintenance of the epidermal barrier, but most of the current in vitro keratinocyte culture systems are linoleic acid-deficient. The aim of the present study was to examine the efficiency of linoleic acid uptake in human keratinocyte cultures grown under submerged and ai...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050430
更新日期：1999-07-01 00:00:00
abstract：:Painting of 3,3',4',5-tetrachlorosalicylanilide (TCSA) plus ultraviolet A (UVA) irradiation to the same site induces contact photosensitivity (CPS), but at the same time results in death of the photohapten-modified cells. Using an in vitro immune lymph node cell (LNC) proliferation system, we investigated the mechanis...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00372063
更新日期：1991-01-01 00:00:00
abstract：:Stratum corneum forms the UV barrier. The effect of ultraviolet B (UVB) on normal skin was extensively studied; however, its effect on barrier perturbed skin remains undefined. Both barrier perturbation and UVB irradiation induce endoplasmic reticulum (ER) stress and unfolded protein response (UPR) in keratinocytes. M...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-017-1753-0
更新日期：2017-09-01 00:00:00
abstract：:The deposition of immunoglobulin (Ig) at the dermo-epidermal junction (DEJ) of the skin, frequently observed in autoimmune mouse strains, is similar to that seen in patients with systemic lupus erythematosus (SLE). MRL/Mp-lpr/lpr (MRL/lpr) mice have an autosomal recessive mutant gene, lpr, which produces massive T-cel...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00370818
更新日期：1993-01-01 00:00:00
abstract：:One of the primary events in the pathogenesis of acne vulgaris is abnormal follicular keratinization. Since oral isotretinoin therapy reduces follicular hyperkeratinization in acne, our study has been designed to determine whether epidermal lipid composition of the epithelium of sebaceous follicles is affected by isot...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00417712
更新日期：1988-01-01 00:00:00
abstract：:Mycosis fungoides was observed in a 71-year-old male with Mediterranean lymphoma, a B-cell malignancy. It is proposed that this association is not incidental since hypergammaglobulinaemia and even monoclonal gammopathies have repeatedly been described in cutaneous T-cell lymphomas. Mediterranean lymphoma might have re...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00417145
更新日期：1981-01-01 00:00:00
abstract：:The expression and location of the Ki-67 antigen was investigated in 62 basal cell carcinomas (BCC) using immunostaining techniques (PAP and APAAP) on cryostat sections. The tumor samples were classified into three groups according to microarchitecture (nodular, superficial or fibrosing). The Ki-67 growth fraction dis...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00371599
更新日期：1993-01-01 00:00:00
abstract：:Although cell death by apoptosis has been recognized as an important control mechanism in the maintenance of tissue homeostasis and in the elimination of cells with damaged DNA, information on the induction and characteristics of apoptosis in keratinocytes is rather scarce. Apoptotic mechanisms may play an important r...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF02505277
更新日期：1996-10-01 00:00:00
abstract：:Exposure in utero has been suggested to influence health later in life. The aim of this study was to investigate, if the use of prenatal food supplements was associated with atopic dermatitis in the offspring. Mothers who gave birth in the hospital G. B. Grassi were invited to participate in the study (n = 395). Infor...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-019-01911-2
更新日期：2019-07-01 00:00:00
abstract：:Quantitative studies of the aerobic flora of seven skin sites in 16 volunteers were repeated after 3 months. The total aerobic densities of the sites differed, male carried more organisms than females and also more biotypes. Qualitative differences in the flora could be detected. ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00509062
更新日期：1982-01-01 00:00:00
abstract：:Adult female Wistar rats (n = 48) divided into four groups of 12 were treated orally with 3 mg/kg per day thalidomide, a 0.05% hexachlorobenzene (HCB)--containing diet, with both drugs together and with the vehicles (controls) over periods of 10 and 60 days. The protein and P450 contents and the activities of aminopyr...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00402227
更新日期：1994-01-01 00:00:00
abstract：:Programmed cell death protein-1 (PD-1) is primarily recognized as an inhibitory receptor involved in the regulation of immunological tolerance. However, recent studies have indicated that PD-1/PD-L1 signaling could also regulate the functions of nonimmune cells and may be involved in regulating hair biology. In this s...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-020-02169-9
更新日期：2021-01-05 00:00:00
abstract：:The authors report 2 cases of atypical vitiligo in which they observed 1) "cockade-like" lesions resembling those of "trichrome" vitiligo (from the centre to the periphery, achromic area, hypochromic ring, normal or hyperchromic border), 2) numerous linear achromic lesions corresponding to former excoriations (Koebner...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00455391
更新日期：1978-07-28 00:00:00
abstract：:Tropospheric ozone (O3) is a source of oxidative stress. This study examined the ability of a topical antioxidant (WEL-DS) to inhibit O3-mediated damage in a human epidermal skin model. Four groups of tissues (N = 24) were compared: Group 1 (control) were untreated and unexposed; Group 2 were untreated and exposed to ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-020-02083-0
更新日期：2020-05-08 00:00:00
abstract：:Paraneoplastic pemphigus patients (PNP) develop a group of autoantibodies, among which those against envoplakin and periplakin are almost always found. Epitope mapping has indicated that the linker subdomains of the proteins harbor the major antigenic sites recognized by PNP sera. In order to detect specific autoantib...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-008-0901-y
更新日期：2009-10-01 00:00:00
abstract：:The hair cycling pattern differs between humans and mice: for the former it is mosaic, while the latter show a wave pattern. We focused on the presence or absence of synchronization of the hair cycle based on the distribution of Merkel cells. The three-dimensional distribution and number of Merkel cells in the skin at...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050474
更新日期：2000-04-01 00:00:00
abstract：:Keloid is a fibrotic disease characterized by abnormal accumulation of extracellular matrix (ECM) in the dermis. It is a late spreading skin overgrowth and may be considered a plastic surgeon's nightmare. In nature, curcuminoid is composed of curcumin, demethoxycurcumin (DMC) and bisdemethoxycurcumin (bDMC). Curcumino...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-010-1075-y
更新日期：2010-12-01 00:00:00
abstract：:Apoptosis is an important cofactor in the pathogenesis of a plethora of malignancies. However, little is known about modulation of the expression of bcl gene family in melanocytic tumors. To determine the role of bcl-2, bcl-x and bax in melanocytic tumors we investigated the differential expression of these genes via ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050479
更新日期：2000-05-01 00:00:00
abstract：:A novel point mutation in a patient with vascular type Ehlers-Danlos syndrome is herein reported. The patient experienced pneumothorax and multiple arterial troubles during the past 10 years. The patient's hand skin was atrophic, and a paper-like atrophic scar was seen. The collagen microfibrils from the patient's ski...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-008-0884-8
更新日期：2008-10-01 00:00:00
abstract：:In skin inflammation, vascular endothelial growth factor (VEGF) and CXCL-8/IL-8 play an important role and are produced by activated keratinocytes. Extracts from Ginkgo biloba leaves (GBE), widely used in phytotherapy, have been reported to exert antioxidant and anti-inflammatory properties in the skin. We therefore e...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-009-0979-x
更新日期：2010-04-01 00:00:00
abstract：:The penetration and permeation of the recombinant protein plasminogen activator inhibitor type 2 (PAI-2) in two formulations, one containing a penetration enhancer, into the psoriatic and uninvolved skin of eight patients with plaque-type psoriasis were investigated. Penetration and permeation of PAI-2 were measured b...
journal_title：Archives of dermatological research
pub_type： 临床试验,杂志文章
doi：10.1007/s00403-003-0425-4
更新日期：2003-11-01 00:00:00
abstract：:The incidence of cutaneous malignant melanoma continues to rise. Strategies to reduce mortality in this situation include improving therapy, encouraging earlier diagnosis, and promoting prevention. New developments such as epiluminescence, sentinel node biopsy, arterial limb perfusion and the use of vaccines are discu...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/BF00370712
更新日期：1994-01-01 00:00:00
abstract：:In human skin, epidermal differentiation occurs in two ways: normal differentiation, characterized by keratin 10 expression, and alternative differentiation. Alternative differentiation can be hyperproliferation-associated differentiation (expression of keratin 16) or the reinduction of an embryonic type of differenti...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/pl00007455
更新日期：2000-01-01 00:00:00
abstract：:Abnormal dermal deposition of elastic fibers is the earliest and most striking effect of prolonged sun exposure (solar elastosis). The hyperplastic fibers are usually ascribed to ultraviolet (UV) rays. Nonetheless, other portions of the solar spectrum may play contributing roles. Heat, for example, enhances experiment...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00509050
更新日期：1982-01-01 00:00:00
abstract：:Secretory component (SC) acts as a transmembrane polymeric immunoglobulin receptor of epithelial cells and is known to bind to polymeric IgA and to contribute to the secretion of secretory IgA (sIgA). We describe a new biological function for free SC (FSC) by which the expression of intercellular adhesion molecule-1 (...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00374074
更新日期：1995-01-01 00:00:00
