Abstract：
:Apoptosis is an important cofactor in the pathogenesis of a plethora of malignancies. However, little is known about modulation of the expression of bcl gene family in melanocytic tumors. To determine the role of bcl-2, bcl-x and bax in melanocytic tumors we investigated the differential expression of these genes via RT-PCR in tissue samples from human benign nevi, primary melanomas and melanoma metastases in comparison with normal skin. Bcl-2 was strongly expressed in 14/16 metastases (87.5%), whereas only 7/13 primary melanomas (53%), 7/15 nevi (46%) and 7/16 normal tissue samples (43%) showed expression of bcl-2 (P < 0.05). There was a strong indication of a correlation between tumor thickness and bcl-2 expression in nodular malignant melanomas. Expression of bcl-x was found in 16/16 melanoma metastases (100%), 11/13 primary melanomas (84%), 12/15 nevi (80%) and 10/16 normal tissue samples (62%) (P < 0.05). Bcl-xL expression increased from primary melanoma to melanoma metastases, whereas bcl-xS showed a decreasing expression level during melanoma progression. No differences in bax expression were seen between melanoma metastases, primary melanoma, nevi and normal tissue. Immunohistochemical investigations of another 53 tissue samples showed similar results. Our results strongly indicate that bcl-2 and bcl-xL gene expression increases with progression of malignant melanoma. Bcl-2 and bcl-xL expression could reflect an increased malignant potential caused by an inhibition of apoptosis and growth advantage for metastatic melanoma cells.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Leiter U,Schmid RM,Kaskel P,Peter RU,Krähn Gdoi
10.1007/s004030050479keywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
225-32issue
5eissn
0340-3696issn
1432-069Xjournal_volume
292pub_type
杂志文章abstract：:In many skin diseases such as Demodex folliculitis, rosacea- or steroid-induced rosacea Demodex mites are present in abundance and are at least partially held responsible for causing these disorders. Although it is known that these diseases respond well to tetracyclines, it is unclear if this is due to the antiinflamm...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-009-0984-0
更新日期：2009-10-01 00:00:00
abstract：:Centromere protein B (CENP-B), which is an alphoid DNA binding protein, is the target antigen in autoimmune disease patients (often those with scleroderma). In this study, we analysed activities of anti-CENP-B-DNA complex in anticentromere antibody (ACA)-positive patients using DNA immunoprecipitation with purified CE...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00372069
更新日期：1992-01-01 00:00:00
abstract：:Wound infections with multi-drug resistant bacteria increase morbidity and mortality and have considerable socioeconomic impact. They can lead to impaired wound healing, resulting in rising treatment costs. The aim of this study was to investigate an ex vivo human wound infection model. Human full-thickness skin from ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-009-1009-8
更新日期：2010-07-01 00:00:00
abstract：:Little attention has been given to the involvement of sweat glands/ducts in the pathogenesis of prurigo nodularis (PN). According to recent studies, PN is likely to develop under conditions characterized by dry skin, such as atopic dermatitis (AD), suggesting a strong impact of skin dryness on PN development. No thera...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-019-01937-6
更新日期：2019-09-01 00:00:00
abstract：:The beta-adrenergic receptors, previously shown to be present on the membranes of cultured human epidermal keratinocytes, were found to be functionally coupled to membrane-bound adenylate cyclase. Using membrane preparations, the enzyme could be activated by guanosine triphosphate (GTP), the stable GTP analog GPP(HN)p...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00407740
更新日期：1986-01-01 00:00:00
abstract：:Skin fibrosis, characterized by excessive fibroblast proliferation and extracellular matrix deposition in the dermis, is the histopathologic hallmark of dermatologic diseases such as systemic sclerosis, hypertrophic scars, and keloids. Effective anti-scarring therapeutics remain an unmet need, underscoring the complex...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/s00403-019-01972-3
更新日期：2020-03-01 00:00:00
abstract：:Reactive oxygen species (ROS) play important roles in the process of ultraviolet-induced skin damage or photoaging. Although many enzymatic and chemical methods have been developed for evaluating ROS, evaluation methods for ROS generation in living systems are quite limited. Here we propose a unique system to visualiz...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-007-0804-3
更新日期：2008-04-01 00:00:00
abstract：:Cutaneous adverse drug reactions (ADRs) are common. However, no prospective study assessing cutaneous ADRs is available for Chinese populations. This study aimed to assess the incidence, manifestations, causative drugs, and other factors related to cutaneous ADRs. A total of 22,866 inpatients were surveyed prospective...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-015-1589-4
更新日期：2015-11-01 00:00:00
abstract：:Eighteen patients with atopic dermatitis were randomly chosen for a clinical trial to compare the action of the H2 receptor antagonist cimetidine in combination with the H1 receptor antagonist chorpheniramine against the chlorpheniramine alone and against placebo. Each treatment period lasted for 4 weeks. Intensity of...
journal_title：Archives of dermatological research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF00412560
更新日期：1984-01-01 00:00:00
abstract：:The expression and location of the Ki-67 antigen was investigated in 62 basal cell carcinomas (BCC) using immunostaining techniques (PAP and APAAP) on cryostat sections. The tumor samples were classified into three groups according to microarchitecture (nodular, superficial or fibrosing). The Ki-67 growth fraction dis...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00371599
更新日期：1993-01-01 00:00:00
abstract：:The stimulation or inhibition of adenylate cyclase by hormones and chemicals is mediated by the stimulatory or inhibitory guanine nucleotide-binding proteins, Gs and Gi respectively. Although the presence of these G-proteins in the epidermis has been suggested, no direct information regarding their nature has been ava...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00372090
更新日期：1990-01-01 00:00:00
abstract：:The aim of the present study was to investigate the influence of three different retinoids, isotretinoin, etretinate and acitretin, on the mitogenic response of peripheral blood mononuclear cells (PBMCs) to PHA and PMA in vitro. All three retinoids at high concentrations (10(-4)M) significantly inhibited the mitogenic...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050291
更新日期：1998-04-01 00:00:00
abstract：:In human skin, epidermal differentiation occurs in two ways: normal differentiation, characterized by keratin 10 expression, and alternative differentiation. Alternative differentiation can be hyperproliferation-associated differentiation (expression of keratin 16) or the reinduction of an embryonic type of differenti...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/pl00007455
更新日期：2000-01-01 00:00:00
abstract：:Six patients with delayed pressure urticaria (DPU) applied clobetasol propionate (0.05%) ointment or its base to predetermined test sites on the right and left thigh as part of a randomized, double-blind study. A pressure challenge was administered to each test site at the initial visit and repeated after 3 days and 6...
journal_title：Archives of dermatological research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF01105080
更新日期：1995-01-01 00:00:00
abstract：:Diabetic foot ulcers (DFUs) are chronic wounds with high matrix metalloproteinase (MMP) activity, and are a frequent complication on diabetics. This work studied the expression of selected MMP and tissue inhibitor of metalloproteinases (TIMP) gene family members in DFU and normal skin biopsies, and in vitamin D-treate...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-014-1494-2
更新日期：2014-11-01 00:00:00
abstract：:Tympanic (Tty), esophageal (Tes), forehead, and hand skin temperatures, as well as the forehead evaporation rate were recorded in six men (four suffering from rosacea and two healthy controls) before, during, and after 1 h of warm bath (38 degrees-39 degrees C). During the last 30 min of the bath, the subject's face w...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00424276
更新日期：1989-01-01 00:00:00
abstract：:Recently a new thin layer chromatography plate (Whatman LK 6D) became available which is extremely easy to handle and permits highly reproducible qualitative and quantitative analysis. This plate proved to be of great value for the investigation of skin surface lipids. The use of a fatty acid methyl ester as an intern...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00412383
更新日期：1979-07-30 00:00:00
abstract：:Vitiligo is an acquired depigmentary disorder of the skin, characterized by incomplete penetrance, multiple susceptibility loci and genetic heterogeneity. An immunologic hypothesis is currently advanced as a possible pathogenesis of vitiligo. The cytokines have an important role in pathogenesis of autoimmunity in whic...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-006-0664-2
更新日期：2006-06-01 00:00:00
abstract：:Paraneoplastic pemphigus patients (PNP) develop a group of autoantibodies, among which those against envoplakin and periplakin are almost always found. Epitope mapping has indicated that the linker subdomains of the proteins harbor the major antigenic sites recognized by PNP sera. In order to detect specific autoantib...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-008-0901-y
更新日期：2009-10-01 00:00:00
abstract：:Three per cent boric acid incorporated in an anhydrous, water-emulsifying ointment causes no increase of boron levels in blood and urine during a period of 1-9 days after a single topical application. The same amount of boric acid incorporated in a water-based jelly does cause an increase in blood and urine levels, be...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00510389
更新日期：1982-01-01 00:00:00
abstract：:Up to now, bicomponent keratohyalin has only been described for rat epithelium and human intraepidermal sweat ducts and fetal nail organ cells. In normal human interductal epidermis, the keratohyalin appears homogeneous, osmiophilic and stellate in shape. Under pathological conditions, bicomponent keratohyalin has bee...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00493461
更新日期：1990-01-01 00:00:00
abstract：:Metalloproteinase-mediated proteolysis plays an important role during all stages of wound repair. In acute murine wounds, murine collagenase, matrix metalloproteinase (MMP)-9, stromelysin-1, stromelysin-2 and tissue inhibitor of metalloproteinase (TIMP)-1 are strongly induced within 24 h after wounding. At the time of...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/pl00007450
更新日期：1998-07-01 00:00:00
abstract：:Based on reports suggesting aberrant cell-mediated immunity and altered infiltration of immunocompetent cells into the skin in psoriasis, we studied the stimulation of T cells by autologous non-T mononuclear leukocytes (autologous mixed lymphocyte reaction, AMLR) and by epidermal cells isolated from lesional and clini...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00417528
更新日期：1986-01-01 00:00:00
abstract：:Propylene glycol (propane-1,2-diol) is the only diol widely used in dermatology. Pentane-1,5-diol is mainly used as a plasticizer in cellulose products and adhesives, in dental composites and in brake fluid compositions and as a preservative for grain. However, pentane-1,5-diol is also an effective solvent, water-bind...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-005-0610-8
更新日期：2005-12-01 00:00:00
abstract：:A low molecular mass cysteine proteinase inhibitor was found and isolated from bovine hoof. Purification of the inhibitor to homogeneity was performed by carboxymethyl-papain-sepharose affinity and DE-52 ion exchange chromatographies. From 15 g of bovine hoof, 0.25 mg of cysteine proteinase inhibitor was isolated. It ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF02505240
更新日期：1996-07-01 00:00:00
abstract：:The aim of this study was to characterize the swelling behaviour of the stratum corneum. Stratum corneum pieces isolated from the breast region of 20 different females were incubated in distilled water at two different temperatures (20 degrees C and 45 degrees C) for 90 min and 24 h, respectively. Half of the stratum ...
journal_title：Archives of dermatological research
pub_type： 临床试验,杂志文章
doi：10.1007/s004030050231
更新日期：1997-08-01 00:00:00
abstract：:The use of microalgae in the skin care market is already established although the scientific rationale for their benefit was not clearly defined. In this work, the biological activities of dermatologic interest of the water extract from the microalga Botryococcus braunii (BBWE) were evaluated by a battery of in vitro ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-012-1250-4
更新日期：2012-11-01 00:00:00
abstract：:Recently, we could show that the expression levels of the key regulators of the microRNA (miRNA) maturation and transport were dysregulated in inflamed hidradenitis suppurativa (HS) tissue (Heyam et al. in Wiley Interdiscip Rev RNA 6:271-289, 2015). The RNA-induced silencing complex (RISC) is the central element of th...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-017-1752-1
更新日期：2017-09-01 00:00:00
abstract：:The treatment of drug-induced toxic epidermal necrolysis (TEN) remains unsatisfactory. Intravenous immunoglobulins (IVIg) and intravenous cyclosporin A (CsA) have shown some efficacy in short series of patients. We assessed the effects of IVIg and CsA on TEN lesional and apparently uninvolved skin using standard histo...
journal_title：Archives of dermatological research
pub_type： 临床试验,杂志文章
doi：10.1007/s00403-005-0607-3
更新日期：2005-12-01 00:00:00
abstract：:Thrombin has recently been shown not only to exert procoagulant activities, but also to induce mitogenic responses of different cell types involved in wound healing via binding to and cleavage of the thrombin receptor. In order to further explore these aspects of thrombin function, human keratinocytes (HaCaT cell line...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030000168
更新日期：2000-10-01 00:00:00
