Abstract:
:Diabetic foot ulcers (DFUs) are chronic wounds with high matrix metalloproteinase (MMP) activity, and are a frequent complication on diabetics. This work studied the expression of selected MMP and tissue inhibitor of metalloproteinases (TIMP) gene family members in DFU and normal skin biopsies, and in vitamin D-treated keratinocytes cultured from those biopsies. We report for the first time the expression of some of these genes in healthy skin. Our results suggest that vitamin D may modulate the expression of some MMP gene family members in keratinocytes. Gene expression in DFU and in non-diabetic healthy skin (control) biopsies was evaluated by RT-qPCR for MMP-1, MMP-3, MMP-8, MMP-9, MMP-10, MMP-19, TIMP-1 and TIMP-2, and also by immunohistochemistry for MMP-1 and MMP-9. Primary keratinocytes cultured from DFU and healthy skin biopsies were used for gene expression analyses of selected MMPs and TIMPs by RT-qPCR, both in the presence and absence of calcitriol. The expression of MMP-1, MMP-8, MMP-9, MMP-10, and TIMP-2 in healthy skin is reported here for the first time. DFUs showed increased MMP-1, MMP-9 and TIMP-1 expression, compared to healthy skin. Calcitriol down-regulated MMP-1 and MMP-10 expression in DFU-derived keratinocytes but not in those derived from healthy skin. Our data demonstrate the expression of certain MMPs that had not been previously described in healthy skin, and further support previous reports of MMP and TIMP up-regulation in DFUs. Our results point to calcitriol as a potential modulator for the expression of certain MMP members in DFUs.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
López-López N,González-Curiel I,Treviño-Santa Cruz MB,Rivas-Santiago B,Trujillo-Paez V,Enciso-Moreno JA,Serrano CJdoi
10.1007/s00403-014-1494-2subject
Has Abstractpub_date
2014-11-01 00:00:00pages
809-21issue
9eissn
0340-3696issn
1432-069Xjournal_volume
306pub_type
杂志文章abstract::Estrone (E1)-sulfatase and dehydroepiandrosterone (DHEA)-sulfatase activities were studied in human female epidermis. Skin specimens were obtained by abdominal or plantar biopsies. The apparent Michaelis-Menten constants for E1 and DHEA sulfatases were 35.2 microM and 8.7 microM, respectively. A substrate inhibition w...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00404316
更新日期:1985-01-01 00:00:00
abstract::In this study we investigated the influence of keratinocytes on the phenotype of fibroblasts in an in vitro human skin equivalent. Keratinocytes were seeded at the surface of fibroblast-populated mechanically restrained type I collagen gels (lattices). Lattices without keratinocytes were handled in parallel as control...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00371739
更新日期:1995-01-01 00:00:00
abstract::We previously showed that the homeodomain protein HOXC13 is involved in the expression control of the early human hair keratin genes hHa5 and hHa2, which contain specific HOXC13 binding sites in their proximal promoters. Hox specificity is generally thought to be enhanced by the interaction with members of the TALE su...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-005-0623-3
更新日期:2006-02-01 00:00:00
abstract::Exposure to a dry environment leads to depletion of water from the peripheral stratum corneum layers in a process dependent on the relative humidity (RH) and the intrinsic properties of the tissue. We hypothesized that by modulating the water content of the stratum corneum in the surface layers, RH effects the rate of...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030100257
更新日期:2001-09-01 00:00:00
abstract::Six patients with delayed pressure urticaria (DPU) applied clobetasol propionate (0.05%) ointment or its base to predetermined test sites on the right and left thigh as part of a randomized, double-blind study. A pressure challenge was administered to each test site at the initial visit and repeated after 3 days and 6...
journal_title:Archives of dermatological research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF01105080
更新日期:1995-01-01 00:00:00
abstract::When determined in vitro, the SPF of certain commercial sunscreen products can be lower than the SPF indicated on the label. The study of the composition of this type of product enabled us to note that the majority contained substances reputed to have anti-inflammatory properties. This effect is shown by inhibiting th...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-012-1257-x
更新日期:2012-12-01 00:00:00
abstract::This study focused on the association between plaque psoriasis and polymorphisms of several inflammation genes. Included in the study were 142 Caucasian (Czech) patients with plaque psoriasis and 141 healthy subjects. The genotypes of the polymorphisms in angiotensinogen [M235T ATG, A(-6)G ATG], in transporters associ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030000176
更新日期:2000-11-01 00:00:00
abstract::In skin inflammation, vascular endothelial growth factor (VEGF) and CXCL-8/IL-8 play an important role and are produced by activated keratinocytes. Extracts from Ginkgo biloba leaves (GBE), widely used in phytotherapy, have been reported to exert antioxidant and anti-inflammatory properties in the skin. We therefore e...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-009-0979-x
更新日期:2010-04-01 00:00:00
abstract::We investigated the effects of glucocorticoids on IL-3-dependent proliferation and c-kit expression of cells of the mouse mast cell line, MC/9. Glucocorticoids (dexamethasone, prednisolone, and hydrocortisone) inhibited IL-3-dependent MC/9 cell proliferation, whereas sex steroids (progesterone, beta-estradiol, and tes...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050398
更新日期:1999-04-01 00:00:00
abstract::The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) and epidermal growth factor (EGF) on type-IV-procollagen (basement-membrane collagen) and laminin synthesis, turnover, and secretion was studied in human A431 squamous epidermoid carcinoma cells. Type-IV procollagen and laminin were biochemically and immunologic...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00509237
更新日期:1985-01-01 00:00:00
abstract::The incidence of cutaneous malignant melanoma continues to rise. Strategies to reduce mortality in this situation include improving therapy, encouraging earlier diagnosis, and promoting prevention. New developments such as epiluminescence, sentinel node biopsy, arterial limb perfusion and the use of vaccines are discu...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/BF00370712
更新日期:1994-01-01 00:00:00
abstract::Tympanic (Tty), esophageal (Tes), forehead, and hand skin temperatures, as well as the forehead evaporation rate were recorded in six men (four suffering from rosacea and two healthy controls) before, during, and after 1 h of warm bath (38 degrees-39 degrees C). During the last 30 min of the bath, the subject's face w...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00424276
更新日期:1989-01-01 00:00:00
abstract::It has been reported that artificial restoration of barrier function by a water vapor-impermeable membrane after tape stripping induces barrier abrogation in hairless mice, impeding rather than enhancing barrier recovery. To address this issue, we examined the morphologic changes in the epidermis after tape stripping ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050280
更新日期:1998-03-01 00:00:00
abstract::This study probes the extent of coupling between the reversal of epidermal hyperproliferation induced by essential fatty acid (EFA) deficiency in guinea pigs, the hydrolysis of epidermal phosphatidylinositol 4,5-bisphosphate (PtdIns4,5P2), and the rapid formation of inositol triphosphate (InsP3). Our data revealed tha...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00440602
更新日期:1988-01-01 00:00:00
abstract::Tumor-associated antigens were demonstrated in phenol water extracts of human malignant melanoma by migration tests with leukocytes from melanoma patients and controls: in 25 out of 60 melanoma patients weak reactivity was observed, usually stimulation of leukocyte migration, while leukocytes from 37 controls were neg...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417277
更新日期:1979-02-23 00:00:00
abstract::Dopamine causes reflex erythema, central blanching, and piloerection depending on the dose and the type of application wheal reaction. Intracutaneous application shows from 1.5 gamma/0.1 ml wheal formation, erythema, piloerection, and blanching combined with increased heat radiation from the skin surface (AGA thermovi...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00412863
更新日期:1979-08-01 00:00:00
abstract::In the case of viral acanthomas, the stratum spinosum and granulosum presents ballooned cells which contain all transitional stages from multivesicular bodies (MVB) to keratinosomes. A particularity in condylomata acuminata are the "wagon-wheel" bodies. These structures are typical for the non keratinazed squamous epi...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::Bone morphogenetic proteins (BMPs) and activins are phylogenetically conserved proteins, belonging to the transforming growth factor-β superfamily, that signal through the phosphorylation of receptor-regulated Smad proteins, activating different cell responses. They are involved in various steps of skin morphogenesis ...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/s00403-013-1381-2
更新日期:2013-09-01 00:00:00
abstract::To examine the induction and repair of UV-induced DNA damage, indirect immunofluorescence was performed on UVB-irradiated organ-cultured normal human skin using monoclonal antibodies specific for either cyclobutane pyrimidine dimers or (6-4) photoproducts. Nuclear immunofluorescence of cyclobutane pyrimidine dimers an...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00375800
更新日期:1992-01-01 00:00:00
abstract::The hair cycling pattern differs between humans and mice: for the former it is mosaic, while the latter show a wave pattern. We focused on the presence or absence of synchronization of the hair cycle based on the distribution of Merkel cells. The three-dimensional distribution and number of Merkel cells in the skin at...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050474
更新日期:2000-04-01 00:00:00
abstract::Atopic dermatitis (AD) is a chronic inflammatory skin disease that affects the patients' quality of life greatly often from a very young age. Its worldwide incidence in children and adults varies, but it is usually among the first ten causes of dermatological consultation worldwide. There is a wide variety of treatmen...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-017-1791-7
更新日期:2018-01-01 00:00:00
abstract::An animal experiment is presented in which two groups of pigmented hairless mice were exposed daily to suberythemal doses of UVA to study tumourigenesis. The aim of the study was to estimate the carcinogenic risks of tanning by UVA. The pigmented hairless mice, Skh-hr2, were separated by selective breeding into two gr...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00375721
更新日期:1990-01-01 00:00:00
abstract::An ultrastructural study of the skin lesion of a young patient affected by pseudo-Kaposi's sarcoma of the Bluefarb-Stewart type (BSS) is reported. The neoplasm consisted of a proliferation of vascular structures mostly consisting of a solid bud of endothelial cells surrounded by a thinned and polystratified basement m...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00424270
更新日期:1989-01-01 00:00:00
abstract::Abnormal dermal deposition of elastic fibers is the earliest and most striking effect of prolonged sun exposure (solar elastosis). The hyperplastic fibers are usually ascribed to ultraviolet (UV) rays. Nonetheless, other portions of the solar spectrum may play contributing roles. Heat, for example, enhances experiment...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00509050
更新日期:1982-01-01 00:00:00
abstract::Little attention has been given to the involvement of sweat glands/ducts in the pathogenesis of prurigo nodularis (PN). According to recent studies, PN is likely to develop under conditions characterized by dry skin, such as atopic dermatitis (AD), suggesting a strong impact of skin dryness on PN development. No thera...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-019-01937-6
更新日期:2019-09-01 00:00:00
abstract::In order to improve the characterization of human malignant melanoma cells and their variant gene expression in vitro, a search for specifically regulated genes was performed. Four melanoma cell lines (M5, MEWO, IGR39, SKMEL13) and newly cultured normal human melanocytes were included in a comparative hybridization (d...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00373422
更新日期:1995-01-01 00:00:00
abstract::The established mouse epidermis-derived cell line HEL/30 was incubated in the presence of 3H arachidonic acid (AA) for 1 h. After medium removal, cells were reincubated with fresh medium in the presence or absence of the calcium ionophore A23187 and tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA). The AA me...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00429984
更新日期:1988-01-01 00:00:00
abstract::In the present study, attempts were made to clarify the role of an elastase-like enzyme in Arthus reaction by using rabbit-peritoneal polymorphonuclear leukocytes (PMNs). It was first confirmed that the injection of large amounts of PMNs with antibody can increase tissue damage in reversed Arthus reaction. The five va...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00413357
更新日期:1984-01-01 00:00:00
abstract::A novel point mutation in a patient with vascular type Ehlers-Danlos syndrome is herein reported. The patient experienced pneumothorax and multiple arterial troubles during the past 10 years. The patient's hand skin was atrophic, and a paper-like atrophic scar was seen. The collagen microfibrils from the patient's ski...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-008-0884-8
更新日期:2008-10-01 00:00:00
abstract::The dependence of epithelial spread on human serum in culture has been studied. Using measured pieces of mouse ear skin epithelial outgrowth about floating explants (epiboly) and from adherent explants was studied. Where compared directly the two systems show similar results. Because of its ease of quantitation, the a...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417274
更新日期:1979-02-23 00:00:00