Abstract:
:Current approaches to monitor and quantify cell division in live cells, and reliably distinguish between acytokinesis and endoreduplication, are limited and complicate determination of stem cell pool identities. Here we overcome these limitations by generating an in vivo reporter system using the scaffolding protein anillin fused to enhanced green fluorescent protein, to provide high spatiotemporal resolution of mitotic phase. This approach visualizes cytokinesis and midbody formation as hallmarks of expansion of stem and somatic cells, and enables distinction from cell cycle variations. High-resolution microscopy in embryonic heart and brain tissues of enhanced green fluorescent protein-anillin transgenic mice allows live monitoring of cell division and quantitation of cell cycle kinetics. Analysis of cell division in hearts post injury shows that border zone cardiomyocytes in the infarct respond with increasing ploidy, but not cell division. Thus, the enhanced green fluorescent protein-anillin system enables monitoring and measurement of cell division in vivo and markedly simplifies in vitro analysis in fixed cells.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Hesse M,Raulf A,Pilz GA,Haberlandt C,Klein AM,Jabs R,Zaehres H,Fügemann CJ,Zimmermann K,Trebicka J,Welz A,Pfeifer A,Röll W,Kotlikoff MI,Steinhäuser C,Götz M,Schöler HR,Fleischmann BKdoi
10.1038/ncomms2089subject
Has Abstractpub_date
2012-01-01 00:00:00pages
1076issn
2041-1723pii
ncomms2089journal_volume
3pub_type
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