Abstract：
:Non-coding RNAs are dominant in the genomic output of the higher organisms being not simply occasional transcripts with idiosyncratic functions, but constituting an extensive regulatory network. Among all the species of non-coding RNAs, small non-coding RNAs (miRNAs, siRNAs and piRNAs) have been shown to be in the core of the regulatory machinery of all the genomic output in eukaryotic cells. Small non-coding RNAs are produced by several pathways containing specialized enzymes that process RNA transcripts. The mechanism of action of these molecules is also ensured by a group of effector proteins that are commonly engaged within high molecular weight protein-RNA complexes. In the last decade, the contribution of structural biology has been essential to the dissection of the molecular mechanisms involved in the biosynthesis and function of small non-coding RNAs.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Costa MC,Leitão AL,Enguita FJdoi
10.3390/ijms130810268subject
Has Abstractpub_date
2012-01-01 00:00:00pages
10268-95issue
8issn
1422-0067pii
ijms-13-10268journal_volume
13pub_type
杂志文章,评审abstract：:For a long time, the nuclear lamina was thought to be the sole scaffold for the attachment of chromosomes to the nuclear envelope (NE) in metazoans. However, accumulating evidence indicates that nuclear pore complexes (NPCs) comprised of nucleoporins (Nups) participate in this process as well. One of the Nups, Elys, i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21249475
更新日期：2020-12-13 00:00:00
abstract：:Previously, we showed that lipocalin2 (LCN2) serum levels increased after liver irradiation and during acute-phase conditions. Here, we evaluate LCN2 expression and serum levels after single-dose lung irradiation with 25 Gy, percutaneously administered to the lung of randomly-paired male Wistar rats. Due to the concav...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17050637
更新日期：2016-04-28 00:00:00
abstract：:Breast cancer is regarded worldwide as a severe human disease. Various genetic variations, including hereditary and somatic mutations, contribute to the initiation and progression of this disease. The diagnostic parameters of breast cancer are not limited to the conventional protein content and can include newly disco...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20174269
更新日期：2019-08-31 00:00:00
abstract：:Recently, neuromediators such as substance P (SP) have been found to be important factors in tendon homeostasis. Some studies have found SP to be the cause of inflammation and tendinopathy, whereas others have determined it to be a critical component of tendon healing. As demonstrated by these conflicting findings, th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228633
更新日期：2020-11-16 00:00:00
abstract：:Wounding is a serious environmental stress in plants. Oxylipins such as jasmonic acid play an important role in defense against wounding. Mechanisms to adapt to wounding have been investigated in vascular plants; however, those mechanisms in nonvascular plants remain elusive. To examine the response to wounding in Phy...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041417
更新日期：2020-02-19 00:00:00
abstract：:Sensitive molecular assays are critical for coronavirus disease 2019 (COVID-19) diagnosis. Here, we designed and evaluated two single-tube nested (STN) real-time RT-PCR assays, targeting SARS-CoV-2 RdRp/Hel and N genes. Both STN assays had a low limit of detection and did not cross react with other human coronaviruses...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21165674
更新日期：2020-08-07 00:00:00
abstract：:Non-communicable diseases (NCDs) have become the largest contributor to worldwide morbidity and mortality. Among them, cancer and cardiovascular diseases (CVDs) are responsible for a 47% of worldwide mortality. In general, preventive approaches modifying lifestyle are more cost-effective than treatments after disease ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19082305
更新日期：2018-08-06 00:00:00
abstract：:Endoplasmic reticulum (ER) calcium homeostasis plays an essential role in cellular calcium signaling, intra-ER protein chaperoning and maturation, as well as in the interaction of the ER with other organelles. Calcium is accumulated in the ER by sarco/endoplasmic reticulum calcium ATPases (SERCA enzymes) that generate...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21093351
更新日期：2020-05-09 00:00:00
abstract：:Head and neck squamous cell carcinoma (HNSCC) is a solid tumor composed by a genotypically and phenotypically heterogeneous population of neoplastic cells types. High recurrence rate and regional metastases lead to major morbidity and mortality. Recently, many studies have focused on cellular and molecular mechanisms ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18091849
更新日期：2017-08-24 00:00:00
abstract：:The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces cancer cell death with minimal damage to normal cells; however, some cancer cells are resistant to TRAIL. TRAIL resistance may be overcome by agonistic antibodies to TRAIL receptors. In this study, we report the toxic effects of a novel recomb...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20081967
更新日期：2019-04-22 00:00:00
abstract：:The terpolymerization of carbon dioxide (CO₂), propylene oxide (PO), and cyclohexene oxide (CHO) were performed by both random polymerization and block polymerization to synthesize the random poly (propylene cyclohexene carbonate) (PPCHC), di-block polymers of poly (propylene carbonate⁻cyclohexyl carbonate) (PPC-PCHC)...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19123723
更新日期：2018-11-23 00:00:00
abstract：:Cardiac diseases are the predominant cause of human mortality in the United States and around the world. MicroRNAs (miRNAs) are small non-coding RNAs that have been shown to modulate a wide range of biological functions under various pathophysiological conditions. miRNAs alter target expression by post-transcriptional...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms150915891
更新日期：2014-09-09 00:00:00
abstract：:Drought stress is one of the most serious threats to cucumber quality and yield. To gain a good understanding of the molecular mechanism upon water deficiency, we compared and analyzed the RNA sequencing-based transcriptomic responses of two contrasting cucumber genotypes, L-9 (drought-tolerant) and A-16 (drought-sens...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19072067
更新日期：2018-07-16 00:00:00
abstract：:Superoxide dismutase (SOD) has attracted considerable attention on treatment of reactive oxygen species (ROS)-related disorders. We previously conjugated Cu/Zn SOD to O-quaternary chitosan derivatives (O-HTCC) to yield a polymer-enzyme conjugate O-HTCC-SOD that demonstrated superior therapeutic effect to native SOD. T...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18061121
更新日期：2017-05-24 00:00:00
abstract：:Many nutrients are absorbed via Na+ cotransport systems, and therefore it is predicted that nutrient absorption mechanisms require a large amount of luminal Na+. It is thought that Na+ diffuses back into the lumen via paracellular pathways to support Na+ cotransport absorption. However, direct experimental evidence in...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21020376
更新日期：2020-01-07 00:00:00
abstract：:Aronia melanocarpa (Michx.) Ell. belongs to the Rosaceae family. The purpose of this study is to explore the gastroprotective effect of the Aronia melanocarpa hydro-alcoholic extract (AMHAE) against ethanol-induced gastric ulcer in a rat model. Different concentrations (50, 100, and 200 mg/kg) of AMHAE, or 30 mg/kg of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18061195
更新日期：2017-06-05 00:00:00
abstract：:Escherichia coli (E. coli) F18 is the main pathogen responsible for post-weaning diarrhea (PWD) in piglets. Resistance to E. coli F18 depends on the expression of the cognate receptors in the intestinal epithelial cells. However, the molecular mechanism of E. coli F18 resistance in weaned piglets remains unclear. Here...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113301
更新日期：2018-10-24 00:00:00
abstract：:S-equol is a major bacterial metabolite of the soy isoflavone daidzein. It is known to be a phytoestrogen that acts by binding to the nuclear estrogen receptors (ERs) that are expressed in various brain regions, including the cerebellum. However, the effects of S-equol on cerebellar development and function have not y...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205178
更新日期：2019-10-18 00:00:00
abstract：: Adenosine monophosphate-activated protein kinase (AMPK) is an important energy sensor which is activated by increases in adenosine monophosphate (AMP)/adenosine triphosphate (ATP) ratio and/or adenosine diphosphate (ADP)/ATP ratio, and increases different metabolic pathways such as fatty acid oxidation, glucose trans...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18020288
更新日期：2017-01-29 00:00:00
abstract：:The neuronal ceroid lipofuscinoses (NCLs) collectively constitute one of the most common forms of inherited childhood-onset neurodegenerative disorders. They form a heterogeneous group of incurable lysosomal storage diseases that lead to blindness, motor deterioration, epilepsy, and dementia. Traditionally the NCL dis...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21218055
更新日期：2020-10-29 00:00:00
abstract：:Leuconostoc mesenteroides DRP105 isolated from Chinese sauerkraut juice is an intensive producer of dextran. We report the complete genome sequence of Leu. mesenteroides DRP105. This strain contains a dextransucrase gene (dsr) involved in the production of dextran, possibly composed of glucose monomers. To explore the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21186596
更新日期：2020-09-09 00:00:00
abstract：:Sleep remains one of the least understood phenomena in biology, and sleep disturbances are one of the most common behavioral problems in childhood. The etiology of sleep disorders is complex and involves both genetic and environmental factors. Epilepsy is the most popular childhood neurological condition and is charac...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20215483
更新日期：2019-11-04 00:00:00
abstract：:Peptide/protein aggregation is implicated in many amyloid diseases. Some amyloidogenic peptides/proteins, such as those implicated in Alzheimer's and Parkinson's diseases, contain multiple amyloidogenic domains connected by "linker" sequences displaying high propensities to form turn structures. Recent studies have de...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms131012169
更新日期：2012-09-25 00:00:00
abstract：:Streptococcus pneumoniae remains the most common bacterial pathogen causing lower respiratory tract infections and is a leading cause of morbidity and mortality worldwide, especially in children and the elderly. Another important aspect related to pneumococcal infections is the persistent rate of penicillin and macrol...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20174220
更新日期：2019-08-28 00:00:00
abstract：:Pea (Pisum sativum L.), as a major source of plant protein, is becoming one of the major cultivated crop species worldwide. In pea, the pericarp is an important determinant of the morphological characteristics and seed yield. To investigate the molecular mechanism of pericarp elongation as well as sucrose and starch a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20246135
更新日期：2019-12-05 00:00:00
abstract：:The paper studies the combined effects of beta-emitting radionuclide tritium and Humic Substances (HS) on the marine unicellular microorganism-luminous bacteria-under conditions of low-dose radiation exposures (<0.04 Gy). Tritium was used as a component of tritiated water. Bacterial luminescence intensity was consider...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21186783
更新日期：2020-09-16 00:00:00
abstract：:Autophagy (particularly macroautophagy) is a bulk degradation process used by eukaryotic cells in order to maintain adequate energy levels and cellular homeostasis through the delivery of long-lived proteins and organelles to the lysosome, resulting in their degradation. It is becoming increasingly clear that many of ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20163916
更新日期：2019-08-12 00:00:00
abstract：:Hedgehog (Hh) proteins are prototypical morphogens known to regulate epithelial/mesenchymal interactions during embryonic development. In addition to its pivotal role in embryogenesis, the Hh signaling pathway may be recapitulated in post-natal life in a number of physiological and pathological conditions, including i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20215270
更新日期：2019-10-24 00:00:00
abstract：:Aminolevulinic acid (ALA) is the first metabolite in the heme biosynthesis pathway in humans. In addition to the end product heme, this pathway also produces other porphyrin metabolites. Protoporphyrin (PpIX) is one heme precursor porphyrin with good fluorescence and photosensitizing activity. Because tumors and other...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms161025865
更新日期：2015-10-28 00:00:00
abstract：:Fresh produce is often a source of enterohaemorrhagic Escherichia coli (EHEC) outbreaks. Fimbriae are extracellular structures involved in cell-to-cell attachment and surface colonisation. F9 (Fml) fimbriae have been shown to be expressed at temperatures lower than 37 °C, implying a function beyond the mammalian host....
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249720
更新日期：2020-12-19 00:00:00
