Abstract：
:The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces cancer cell death with minimal damage to normal cells; however, some cancer cells are resistant to TRAIL. TRAIL resistance may be overcome by agonistic antibodies to TRAIL receptors. In this study, we report the toxic effects of a novel recombinant agonistic human anti-TRAIL receptor 1 (DR4) monoclonal antibody Fab fragment, DR4-4, on various TRAIL-resistant and -sensitive cancer cell lines. The mechanisms of DR4-4 Fab-induced cell death in a human T cell leukemia cell line (Jurkat) were investigated using cell viability testing, immunoblotting, immunoassays, flow cytometry, and morphological observation. DR4-4 Fab-induced caspase-independent necrosis was observed to occur in Jurkat cells in association with p38 mitogen-activated protein kinase activation, cellular FLICE (FADD-like IL-1β-converting enzyme)-inhibitory protein degradation, decreased mitochondrial membrane potential, and increased mitochondrial reactive oxygen species production. Increased cytotoxic effects of DR4-4 Fab were observed in combination with TRAIL or γ-irradiation. Our results indicate that the novel DR4-4 Fab might overcome TRAIL-resistance and induce death in leukemia cells via cellular mechanisms different from those activated by TRAIL. DR4-4 Fab may have application as a potential therapeutic antibody fragment in single or combination therapy for cancer.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Lee YR,Hwang E,Jang YJdoi
10.3390/ijms20081967subject
Has Abstractpub_date
2019-04-22 00:00:00issue
8issn
1422-0067pii
ijms20081967journal_volume
20pub_type
杂志文章abstract：:Nicotine addiction is a severe public health problem. The aim of this study was to investigate the alterations in key neurotransmissions after 60 days of withdrawal from seven weeks of intermittent cigarette smoke, e-cigarette vapours, or an e-cigarette vehicle. In the nicotine withdrawal groups, increased depressive ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020599
更新日期：2021-01-09 00:00:00
abstract：:Glutathione Transferases (GSTs) are phase II detoxification enzymes known to be involved in the molecular response against microcystins (MCs) induced toxicity. However, the individual role of the several GST isoforms in the MC detoxification process is still unknown. In this study, the time-dependent changes on gene e...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms16048397
更新日期：2015-04-15 00:00:00
abstract：:This paper presents the synthesis of structured phosphatidylcholine (PC) enriched with docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) by transesterification of DHA/EPA-rich ethyl esters with PC using immobilized phospholipsase A1 (PLA1) in solvent-free medium. Firstly, liquid PLA1 was immobilized on resin ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150915244
更新日期：2014-08-28 00:00:00
abstract：:During their lifespan, Red blood cells (RBC), due to their inability to self-replicate, undergo an ageing degradation phenomenon. This pathway, both in vitro and in vivo, consists of a series of chemical and morphological modifications, which include deviation from the biconcave cellular shape, oxidative stress, membr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19092582
更新日期：2018-08-30 00:00:00
abstract：:Stem/progenitor cells are promising candidates for the regeneration of parenchyma in acute and chronic renal failure. However, recent data exhibit that survival of stem/progenitor cells after implantation in diseased renal parenchyma is restricted. To elaborate basic parameters improving survival, cell seeding was sim...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms151223240
更新日期：2014-12-15 00:00:00
abstract：:Protein splicing catalyzed by inteins utilizes many different combinations of amino-acid types at active sites. Inteins have been classified into three classes based on their characteristic sequences. We investigated the structural basis of the protein splicing mechanism of class 3 inteins by determining crystal struc...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21218367
更新日期：2020-11-07 00:00:00
abstract：:In the central nervous system omega-3 fatty acids modulate cell signaling and affect dopaminergic and serotonergic pathways. On this basis, a new application for omega-3 fatty acids has been proposed, concerning the treatment of several psychiatric disorders. The present article is an update of a previous systematic r...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20215257
更新日期：2019-10-23 00:00:00
abstract：:Multifactorial metabolic diseases, such as non-alcoholic fatty liver disease, are a major burden to modern societies, and frequently present with no clearly defined molecular biomarkers. Herein we used system medicine approaches to decipher signatures of liver fibrosis in mouse models with malfunction in genes from un...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020832
更新日期：2021-01-15 00:00:00
abstract：:Endocrine therapy is a key treatment strategy to control or eradicate hormone-responsive breast cancer. However, resistance to endocrine therapy leads to breast cancer relapse. The recent extension of adjuvant tamoxifen treatment up to 10 years actualizes the need for identifying biological markers that may be used to...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms161024243
更新日期：2015-10-14 00:00:00
abstract：:Nanotechnology has rapidly promoted the development of a new generation of industrial and commercial products; however, it has also raised some concerns about human health and safety. To evaluate the toxicity of the great diversity of nanomaterials (NMs) in the traditional manner, a tremendous number of safety assessm...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21072387
更新日期：2020-03-30 00:00:00
abstract：:Radiolysis of biomolecules by fast ions has interest in medical applications and astrobiology. The radiolysis of solid D-valine (0.2-2 μm thick) was performed at room temperature by 1.5 MeV H+, He+, N+, and 230 MeV S15+ ion beams. The samples were prepared by spraying/dropping valine-water-ethanol solution on ZnSe sub...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051893
更新日期：2020-03-10 00:00:00
abstract：:Abscisic acid (ABA) plays important roles in positively or negatively regulating plant disease resistance to pathogens. Here, we reassess the role of endogenous and exogenous ABA by using: 35S::ABA2, a previously reported transgenic Arabidopsis line with increased endogenous ABA levels; aba2-1, a previously reported A...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20102544
更新日期：2019-05-23 00:00:00
abstract：:Hyperuricemia, or elevated serum urate, causes urate kidney stones and gout and also increases the incidence of many other conditions including renal disease, cardiovascular disease, and metabolic syndrome. As we gain mechanistic insight into how urate contributes to human disease, a clear sex difference has emerged i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21124269
更新日期：2020-06-16 00:00:00
abstract：:Photodynamic inactivation (PDI) is a non-antibiotic option for the treatment of infectious diseases. Although Gram-positive bacteria have been shown to be highly susceptible to PDI, the inactivation of Gram-negative bacteria has been more challenging due to the impermeability properties of the outer membrane. In the p...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20010134
更新日期：2019-01-01 00:00:00
abstract：:Idiopathic osteoporosis and nephrolithiasis are formidable health problems showing a progressive increase in their incidence and prevalence in the last decades. These temporal trends were observed in both pediatric and adult populations worldwide. Epidemiological and experimental studies indicate that both disorders s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21218183
更新日期：2020-10-31 00:00:00
abstract：:Platelet P2Y12 is an important adenosine diphosphate (ADP) receptor that is involved in agonist-induced platelet aggregation and is a valuable target for the development of anti-platelet drugs. Here we characterise the effects of thio analogues of uridine triphosphate (UTP) on ADP-induced platelet aggregation. Using h...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18020269
更新日期：2017-01-29 00:00:00
abstract：:Alpha thalassaemia is highly prevalent in the plural society of Malaysia and is a public health problem. Haematological and molecular data from 5016 unrelated patients referred from various hospitals to the Institute for Medical Research for α thalassaemia screening from 2007 to 2010 were retrieved. The aims of this r...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140918599
更新日期：2013-09-10 00:00:00
abstract：:Mucin1 (MUC1) is a highly glycosylated transmembrane protein that plays a crucial role in the lubrication and protection of normal epithelial cells. However, MUC1 has emerged as a potential target for cancer therapy because it is overexpressed and functions in several types of cancers. Recently, we produced a monoclon...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19072004
更新日期：2018-07-09 00:00:00
abstract：:Human keratinocytes were recently shown to respond to anti-EGFR (epidermal growth factor receptor) drugs with activation of an interferon-κ-driven autocrine loop, leading to enhanced expression of innate antiviral effectors and of the pro-inflammatory chemokines CXCL10 (C-X-C motif chemokine 10) and CCL2 (C-C motif li...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19092652
更新日期：2018-09-06 00:00:00
abstract：:Pancreatic Ductal Adenocarcinoma (PDAC) is one of the most lethal cancers due to a high chemoresistance and poor vascularization, which results in an ineffective systemic therapy. PDAC is characterized by a high intratumoral pressure, which is not captured by current 2D and 3D in vitro models. Here, we demonstrated a ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184647
更新日期：2019-09-19 00:00:00
abstract：:In the past 17 years, three novel coronaviruses have caused severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and the coronavirus disease 2019 (COVID-19). As emerging infectious diseases, they were characterized by their novel pathogens and transmissibility without available clinical d...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21113843
更新日期：2020-05-28 00:00:00
abstract：:Dual drug-loaded nanotherapeutics can play an important role against the drug resistance and side effects of the single drugs. Doxorubicin and sorafenib were efficiently co-encapsulated by tailor-made poly([R,S]-3-hydroxybutyrate) (PHB) using an emulsion-solvent evaporation method. Subsequent poly(ethylene glycol) (PE...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197312
更新日期：2020-10-03 00:00:00
abstract：:Scleroderma is a disorder involving oral and facial tissues, with skin hardening, thin lips, deep wrinkles, xerostomia, tongue rigidity, and microstomia. The aim of this study was to investigate the prevalence of oral manifestations and temporomandibular disorders (TMD) in Systemic Sclerosis (SSc) patients compared wi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,随机对照试验
doi：10.3390/ijms17071189
更新日期：2016-07-22 00:00:00
abstract：:Antibiotics without selectivity for acne treatment may destroy the beneficial microbes in the human microbiome that helps to fight Cutibacterium acnes (C. acnes), a bacterium associated with inflammatory acne vulgaris. Probiotic treatment by direct application of live Staphylococcus epidermidis (S. epidermidis) onto t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20010014
更新日期：2018-12-20 00:00:00
abstract：:Bacteria generate membrane vesicles, which are structures known as extracellular vesicles (EVs), reported to be involved in different pathogenic mechanisms, as it has been demonstrated that EVs participate in biofilm formation, cell-to-cell communication, bacteria-host interactions, and nutrients supply. EVs deliver n...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20215307
更新日期：2019-10-25 00:00:00
abstract：:Endocannabinoids including anandamide and 2-arachidonoylglycerol are involved in cancer pathophysiology in several ways, including tumor growth and progression, peritumoral inflammation, nausea and cancer pain. Recently we showed that the endocannabinoid profiles are deranged during cancer to an extent that this manif...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17020230
更新日期：2016-02-10 00:00:00
abstract：:The objective of our study was to evaluate whether feeding pseudopurpurin affects bone mineral density and bone geometry architecture in rats. Pseudopurpurin was extracted, analyzed and purified using an HLPC-ESI-MS. Rats were given 0% and 0.5% pseudopurpurin powder in their diet. Femurs of rats were examined at 0.5, ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13033431
更新日期：2012-01-01 00:00:00
abstract：:Ionic liquids, considered "green" chemicals, are widely used in many areas of analytical chemistry due to their unique properties. Recently, ionic liquids have been used as a kind of novel additive in separation and combined with silica to synthesize new stationary phase as separation media. This review will focus on ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms10062591
更新日期：2009-06-04 00:00:00
abstract：:The common carp can tolerate extremely low oxygen levels. These fish store zinc in a specific zinc-binding protein presented in digestive tract tissues, and under low oxygen, the stored zinc is released and used as a signal to stimulate erythropoiesis (red blood cell formation). To determine whether the environmental ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18010138
更新日期：2017-01-11 00:00:00
abstract：:A previous report showed that both Pyruvatedecarboxylase (PDC) genes were significantly upregulated in kiwifruit after waterlogging treatment using Illumina sequencing technology, and that the kiwifruit AdPDC1 gene was required during waterlogging, but might not be required during other environmental stresses. Here, t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18112377
更新日期：2017-11-09 00:00:00
