Abstract：
:During their lifespan, Red blood cells (RBC), due to their inability to self-replicate, undergo an ageing degradation phenomenon. This pathway, both in vitro and in vivo, consists of a series of chemical and morphological modifications, which include deviation from the biconcave cellular shape, oxidative stress, membrane peroxidation, lipid content decrease and uncoupling of the membrane-skeleton from the lipid bilayer. Here, we use the capabilities of atomic force microscopy based infrared nanospectroscopy (AFM-IR) to study and correlate, with nanoscale resolution, the morphological and chemical modifications that occur during the natural degradation of RBCs at the subcellular level. By using the tip of an AFM to detect the photothermal expansion of RBCs, it is possible to obtain nearly two orders of magnitude higher spatial resolution IR spectra, and absorbance images than can be obtained on diffraction-limited commercial Fourier-transform Infrared (FT-IR) microscopes. Using this approach, we demonstrate that we can identify localized sites of oxidative stress and membrane peroxidation on individual RBC, before the occurrence of neat morphological changes in the cellular shape.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Ruggeri FS,Marcott C,Dinarelli S,Longo G,Girasole M,Dietler G,Knowles TPJdoi
10.3390/ijms19092582subject
Has Abstractpub_date
2018-08-30 00:00:00issue
9issn
1422-0067pii
ijms19092582journal_volume
19pub_type
杂志文章abstract：BACKGROUND:Ras homolog gene family member A (RhoA) is involved in Wnt-5a-induced migration of gastric and breast cancer cells. We investigated the roles of RhoA and Wnt-5a in ovarian carcinoma. METHODS:RhoA and Wnt-5a mRNA and protein expression in normal fallopian tube epithelium, benign tumors, primary ovarian carci...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms141224187
更新日期：2013-12-12 00:00:00
abstract：:Chenopodium quinoa Wild is a "pseudocereal" grain which attracts a lot of attention in the scientific community as it has a positive effect on health. Here, we investigate the presence of biologically active O-prenylated phenylpropanoids in the ethanol extract of commercially available quinoa seeds. We claim that 4'-G...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20133262
更新日期：2019-07-02 00:00:00
abstract：:Small RNAs are essential for a variety of cellular functions. Argonaute (AGO) proteins are associated with all of the different classes of small RNAs, and are indispensable in small RNA-mediated regulatory pathways. AGO proteins have been identified in various types of stem cells in diverse species from plants and ani...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17020208
更新日期：2016-02-04 00:00:00
abstract：:Kinase drug discovery represents an active area of therapeutic research, with previous pharmaceutical success improving patient outcomes across a wide variety of human diseases. In pancreatic ductal adenocarcinoma (PDAC), innovative pharmaceutical strategies such as kinase targeting have been unable to appreciably inc...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21228823
更新日期：2020-11-21 00:00:00
abstract：:Anthocyanins are the largest class of water soluble plant pigments and a common part of the human diet. They may have many potential health benefits, including antioxidant, anti-inflammatory, anti-cancer, and cardioprotective activities. However, anthocyanin metabolism is not well understood. Studies suggest that anth...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18020446
更新日期：2017-02-18 00:00:00
abstract：:DNA topoisomerase II (TOP2) activity involves a normally transient double-strand break intermediate in which the enzyme is coupled to DNA via a 5'-phosphotyrosyl bond. However, etoposide and other topoisomerase drugs poison the enzyme by stabilising this enzyme-bridged break, resulting in the accumulation of TOP2-DNA ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19072056
更新日期：2018-07-14 00:00:00
abstract：:Small noncoding RNAs (sncRNAs) are key regulators of the majority of human reproduction events. Understanding their function in the context of gametogenesis and embryogenesis will allow insight into the possible causes of in vitro fertilization (IVF) implantation failure. The aim of this study was to analyze the sncRN...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20122912
更新日期：2019-06-14 00:00:00
abstract：:Targeting adenosine triphosphate (ATP) metabolism and adenosinergic signaling in cancer is gaining momentum, as increasing evidence is showing their relevance in tumor immunology and biology. Chronic lymphocytic leukemia (CLL) results from the expansion of a population of mature B cells that progressively occupies the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19041167
更新日期：2018-04-12 00:00:00
abstract：:Glioblastoma is the most common malignant primary brain tumor, and it is one of the causes of cancer fatality in both adult and pediatric populations. Patients with glioblastoma require chemotherapy after surgical resection and radiotherapy. Therefore, chemotherapy constitutes a viable approach for the eradication of ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18091945
更新日期：2017-09-11 00:00:00
abstract：:Fibrosis is presented in various physiologic and pathologic conditions of the salivary gland. Transforming growth factor beta (TGF-β) pathway has a pivotal role in the pathogenesis of fibrosis in several organs, including the salivary glands. Among the TGF-β superfamily members, TGF-β1 and 2 are pro-fibrotic ligands, ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21239138
更新日期：2020-11-30 00:00:00
abstract：:Low-grade inflammation is implicated in the pathogenesis of atherosclerosis, metabolic syndrome, and apathy as a form of vascular depression. We analyzed the brain magnetic resonance imaging findings in 259 community-dwelling older adults (122 men and 137 women, with a mean age of 68.4 years). The serum concentrations...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20081905
更新日期：2019-04-17 00:00:00
abstract：:Understanding molecular interactions and regulatory mechanisms in tumor initiation, progression, and treatment response are key requirements towards advanced cancer diagnosis and novel treatment procedures in personalized medicine. Beyond decoding the gene expression, malfunctioning and cancer-related epigenetic pathw...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18102066
更新日期：2017-09-28 00:00:00
abstract：:Poly-γ-glutamic acid (γ-PGA) is a biodegradable biopolymer produced by several bacteria, including Bacillus subtilis and other Bacillus species; it has good biocompatibility, is non-toxic, and has various potential biological applications in the food, pharmaceutical, cosmetic, and other industries. In this review, we ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18122644
更新日期：2017-12-07 00:00:00
abstract：:The relationship between antiphospholipid antibodies (aPL) and autoimmune haemolytic anaemia (AIHA) has never been systematically addressed. The aim of this study is to assess the link between aPL and AIHA in adult systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). This study performed an EMBASE/P...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21114120
更新日期：2020-06-09 00:00:00
abstract：:Maternal overweight in pregnancy alters the metabolic environment and generates chronic low-grade inflammation. This affects fetal development and programs the offspring's health for developing cardiovascular and metabolic disease later in life. MME (membrane-metalloendopeptidase, neprilysin) cleaves various peptides ...
journal_title：International journal of molecular sciences
pub_type： 临床试验,杂志文章
doi：10.3390/ijms21030834
更新日期：2020-01-28 00:00:00
abstract：:Histamine is a well-known mediator of inflammation that is released from mast cells and basophils. To date, many studies using histamine receptor antagonists have shown that histamine acts through four types of receptors: H1, H2, H3, and H4. Thus, histamine plays more roles in various diseases than had been predicted....
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20020376
更新日期：2019-01-16 00:00:00
abstract：:Platelet-released growth factor (PRGF) is a thrombocyte concentrate lysate which, like its clinically equivalent variations (e.g., Vivostat PRF® (platelet-rich fibrin)), is known to support the healing of chronic and hard-to-heal wounds. However, studies on the effect of PRGF on keratinocytes remain scarce. This study...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124404
更新日期：2020-06-20 00:00:00
abstract：:Peanut samples were irradiated (0.0, 5.2, 7.2 or 10.0 kGy), stored for a year (room temperature) and examined every three months. Mycotoxic fungi (MF) were detected in non-irradiated blanched peanuts. A dose of 5.2 kGy was found suitable to prevent MF growth in blanched samples. No MF was detected in in-shell peanuts,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms130910935
更新日期：2012-01-01 00:00:00
abstract：:Recessively-inherited NR2E3 gene mutations cause an unusual retinopathy with abnormally-increased short-wavelength sensitive cone (S-cone) function, in addition to reduced rod and long/middle-wavelength sensitive cone (L/M-cone) function. Progress toward clinical trials to treat patients with this otherwise incurable ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20102497
更新日期：2019-05-21 00:00:00
abstract：:The eukaryotic and archaeal translation factor IF5A requires a post-translational hypusine modification, which is catalyzed by deoxyhypusine synthase (DHS) at a single lysine residue of IF5A with NAD+ and spermidine as cofactors, followed by hydroxylation to form hypusine. While human DHS catalyzed reactions have been...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21155509
更新日期：2020-07-31 00:00:00
abstract：:Although most cancer types have been viewed as immunologically silent until recently, it has become increasingly clear that the immune system plays key roles in the course of tumor development. Remarkable progress towards understanding cancer immunogenicity and tumor-immune system interactions has revealed important i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17071030
更新日期：2016-06-29 00:00:00
abstract：:Previously, we demonstrated that the immediate administration of multitarget anxiolytic afobazole slows down the progression of neuronal damage in a 6-hydroxidodamine (6-OHDA) model of Parkinson's disease due to the activation of chaperone Sigma1R. The aim of the present study is to evaluate the therapeutic potential ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21207620
更新日期：2020-10-15 00:00:00
abstract：:Muscle contraction is initiated by the interaction between actin and myosin filaments. The sliding of actin filaments relative to myosin filaments is produced by cross-bridge cycling, which is governed by the theoretical framework of the cross-bridge theory. The cross-bridge theory explains well a number of mechanical...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20215479
更新日期：2019-11-04 00:00:00
abstract：:Calcium (Ca2+) is a universal and vital intracellular messenger involved in a diverse range of cellular and biological processes. Changes in the concentration of extracellular Ca2+ can disrupt the normal cellular activities and the physiological function of these systems. The calcium sensing receptor (CaSR) is a uniqu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20215341
更新日期：2019-10-27 00:00:00
abstract：:A series of diketopyrrolopyrrole-based small molecules have been designed to explore their optical, electronic, and charge transport properties as organic solar cell(OSCs) materials. The calculation results showed that the designed molecules can lower the band gap and extend the absorption spectrum towards longer wave...
journal_title：International journal of molecular sciences
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更新日期：2015-08-27 00:00:00
abstract：:Being master regulators of gene expression, transcription factors (TFs) play important roles in determining plant growth, development and reproduction. To date, many TFs have been shown to positively mediate plant responses to environmental stresses. In the current study, the biological functions of a stress-responsiv...
journal_title：International journal of molecular sciences
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doi：10.3390/ijms21010286
更新日期：2019-12-31 00:00:00
abstract：:The authors are sorry to report that some of the HPLC data reported in their recently published paper [...]. ...
journal_title：International journal of molecular sciences
pub_type： 已发布勘误
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更新日期：2019-08-26 00:00:00
abstract：:Liver X receptors (LXRs) play a pivotal role in fatty acid (FA) metabolism. So far, the lipogenic consequences of in vivo LXR activation, as characterized by a major hepatic steatosis, has constituted a limitation to the clinical development of pharmacological LXR agonists. However, recent studies provided a different...
journal_title：International journal of molecular sciences
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更新日期：2019-08-02 00:00:00
abstract：:A multifunctional transgenic Lactobacillus with probiotic characteristics and an ability to degrade β-glucan and phytic acid (phytate) was engineered to improve nutrient utilization, increase production performance and decrease digestive diseases in broiler chickens. The Bacillus subtilis WL001 endoglucanase gene (cel...
journal_title：International journal of molecular sciences
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更新日期：2014-07-21 00:00:00
abstract：:Hepatic ischemia/reperfusion (I/R) injury is a leading cause of organ dysfunction and failure in numerous pathological and surgical settings. At the core of this issue lies mitochondrial dysfunction. Hence, strategies that prime mitochondria towards damage resilience might prove applicable in a clinical setting. A pro...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21144896
更新日期：2020-07-11 00:00:00
